User Manuals: Invitrogen iBlot 2 Gel Transfer Device

User Manuals: Invitrogen iBlot 2 Gel Transfer Device

Manuals and User Guides for Invitrogen iBlot 2 Gel Transfer Device. We have 1 Invitrogen iBlot 2 Gel Transfer Device manual available for free PDF download: User Manual

Invitrogen iBlot 2 User Manual

Invitrogen iBlot 2 User Manual (68 pages)

Dry Blotting System.

Brand: Invitrogen | Category: Laboratory Equipment | Size: 9 MB

Table of Contents

Table of Contents
3
CHAPTER 1 Product Information

6
- Product Contents
  6
- Unpacking Instructions
  8
  - Upon Receiving the Instrument
    8
  - Unpacking the Iblot ™ 2 Gel Transfer Device
    8
- Gel Transfer Device
  8
  - Front View
    8
  - Rear View
    9
  - Side View
    9
- About the System
  9
  - Dry Blotting System
    9
  - Features
    10
  - System Components
    10
  - System Overview
    10
- Description of Parts
  12
  - Gel Transfer Device
    12
  - Blotting Roller
    13
  - Stylus
    14
  - Power Cord
    14
  - Power Adapters
    14
  - Transfer Stacks
    15
  - Guidelines for Iblot
    15
  - Iblot ™ 2 Transfer Stacks
    15
  - Top Stack
    16
  - Bottom Stack
    16
  - Transfer Membrane
    16
  - Filter Paper
    17
  - Absorbent Pad
    17
- Gel Transfer Device
  18
- Description of Methods
  23
  - Methods
    23
  - Recommended Running Parameters
    23
CHAPTER 2 Protein Transfer Protocol

25
- Experimental Overview
  25
  - Experimental Outline
    25
- General Guidelines
  25
  - Recommended Gel Types
    26
- Using the Iblot ™ 2 Gel Transfer Device
  26
  - Introduction
    26
  - Materials Needed
    26
  - Selecting a Method
    27
  - Removing the Gel
    28
  - General Guidelines
    28
  - Assembling the Iblot ™ 2 Transfer Stack
    29
  - Performing Blotting
    33
- Disassembling the Iblot ™ 2 Transfer Stack
  34
  - Introduction
    34
  - Procedure
    34
CHAPTER 3 Custom Methods

36
- Creating Custom Methods
  36
  - Introduction
    36
  - Programming a Custom Method
    36
  - Save a Custom Method
    38
- Creating Custom Methods from a Template
  39
  - Introduction
    39
  - Select a Template
    39
  - Change Voltage/Time
    40
  - Add/Remove Steps
    41
  - Save a Custom Method
    43
APPENDIX A Troubleshooting

45
- Introduction
  45
APPENDIX B Post Transfer Analysis

48
- Post Transfer Analysis
  48
APPENDIX C Optimizing Blotting

49
- Optimizing Blotting
  49
APPENDIX D Maintenance

50
- Cleaning
  50
- General Maintenance
  50
- Replacing the Fuse
  50
- Replacing Electrical Contacts
  51
APPENDIX E Product Specifications

55
APPENDIX F Accessory Products

57
- Transfer Stacks
  57
- Additional Products
  57
- Iblot ™ 2 Transfer Stacks
  57
- Precast Gels and Premade Buffers
  58
APPENDIX G Safety

59
- Instrument Safety
  59
  - General
    59
  - Physical Injury
    59
  - Electrical Safety
    60
  - Cleaning and Decontamination
    61
  - Instrument Component and Accessory Disposal
    61
- Safety and Electromagnetic Compatibility (EMC) Standards
  61
  - Symbols on Instrument
    62
  - Safety Standards
    62
  - Emc
    62
  - Environmental Design Standards
    63
- Chemical Safety
  64
- Biological Hazard Safety
  65
APPENDIX H Documentation and Support

66
- Customer and Technical Support
  66
- Limited Product Warranty
  66

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