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10906
ibidi 10906 Manuals
Manuals and User Guides for ibidi 10906. We have
1
ibidi 10906 manual available for free PDF download: Instruction Manual
ibidi 10906 Instruction Manual (80 pages)
Pump System
Brand:
ibidi
| Category:
Water Pump
| Size: 8 MB
Table of Contents
Table of Contents
3
Preamble
7
Introduction
7
Safety Symbols
7
Nomenclature
8
Specifications
8
Disclaimer
10
Safety Considerations
10
Limited Warranty
12
Transporting the Ibidi Pump System
12
Repairing the Ibidi Pump System
12
Waste Disposal - Weee/Rohs Compliance Statement
13
EU Directive WEEE
13
EU Directive Rohs
13
Regulatory Statement
14
Intended Use of the Ibidi Pump System
15
Equipment
16
Components of the Ibidi Pump System
16
Ibidi Pump
17
Fluidic Unit
18
The Valve Block
20
The Pinch Valve
20
Perfusion Sets
21
Ibidi Μ-Slides
22
Slide and Perfusion Set Selection Guide
25
Drying Bottle
30
Computer with Pumpcontrol Software
31
Basic Setup
33
General Setup of the Components
33
Connecting the Fluidic Unit to the Pump
34
Connecting the Drying Bottle
34
Installing the Pumpcontrol Software
35
Connecting the Ibidi Pump to the Computer
36
Setting up an Experiment with Cells
37
Degassing of Slides, Tubing, and Medium
37
Mounting a Perfusion Set on the Fluidic Unit
38
Filling the Perfusion Set with Medium
41
Remove Air Bubbles from the Perfusion Set
42
Pinch Test
43
Calibration of the Flow Rate
43
Connect the Calibration Slide to the Perfusion Set
44
Flow Rate Measurement
45
Flow Calibration in the Software
47
Connect the Cell-Seeded Slide to the Perfusion Set
48
Start the Flow
48
Observing Cells on the Microscope
48
Medium Exchange
49
Medium Exchange with Left-Over Conditioned Medium (Option 1)
49
Complete Medium Exchange (Option 2)
49
Installation of Special Setups
51
Working with Two or more Fluidic Units
51
Installation of Two or more Fluidic Units
51
Flow Calibration of Two or more Fluidic Units
52
Calibration of the Flow Rate with Several Slides in Serial Connection
52
Oscillatory Flow Experiments
53
Setting up Oscillatory Flow with Two Fluidic Units
53
Settings Within the Pumpcontrol Software
56
Oscillatory Experiment with Four Fluidic Units
56
Pulsatile Flow Experiments
58
Technical Details
60
Working Principle of the Ibidi Pump
60
Positive Versus Negative Air Pressure
62
Flow Characteristics
64
Viscosity
65
Shear Stress Calculations in Ibidi Channel Slides
65
Working with Non-Implemented Flow Channels
68
Maintenance
71
Disinfection and Cleaning
71
Silica Beads from the Drying Bottle
71
Replacement Filters for Perfusion Sets
71
Fluidic Unit Filters
71
Fluidic Unit Pinch Valves
71
Troubleshooting
72
Air Bubbles
72
Air Bubbles Emerging When Connecting the Slide
72
Air Bubbles Emerging after a few Hours
72
Cells Are Detaching
73
Cells Detach before Starting the Flow
73
Cells Detach When Connecting the Slide to the Perfusion Set
73
Cells Detach under Flow Conditions
74
Imbalanced Medium
74
Medium Runs in One Direction Only
75
No Connection between Ibidi Pump and Pumpcontrol Software
76
Pressure Kickback after Pressure Switch off
76
Flow Rate Is too Low or Absent
77
Flow Rate Is too High
77
Total Medium Volume in the Perfusion Set Is Decreasing
78
Flow Direction in the Channel Is Changing
78
Pressure Lost Error
78
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