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ibidi Pump System Instruction Manual

Pump system

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Table Of Contents

Table of Contents

Table of Contents
Preamble
Introduction
Safety Symbols
Nomenclature
Specifications
Disclaimer
Safety Considerations
Limited Warranty
Transporting the Ibidi Pump System
Repairing the Ibidi Pump System
Waste Disposal - Weee/Rohs Compliance Statement
EU Directive WEEE
EU Directive Rohs
Regulatory Statement
Intended Use of the Ibidi Pump System
Equipment
Components of the Ibidi Pump System
Ibidi Pump
Fluidic Unit
The Valve Block
The Pinch Valve
Perfusion Sets
Ibidi Μ-Slides
Slide and Perfusion Set Selection Guide
Drying Bottle
Computer with Pumpcontrol Software
Basic Setup
General Setup of the Components
Connecting the Fluidic Unit to the Pump
Connecting the Drying Bottle
Installing the Pumpcontrol Software
Connecting the Ibidi Pump to the Computer
Setting up an Experiment with Cells
Degassing of Slides, Tubing, and Medium
Mounting a Perfusion Set on the Fluidic Unit
Filling the Perfusion Set with Medium
Remove Air Bubbles from the Perfusion Set
Pinch Test
Calibration of the Flow Rate
Connect the Calibration Slide to the Perfusion Set
Flow Rate Measurement
Flow Calibration in the Software
Connect the Cell-Seeded Slide to the Perfusion Set
Start the Flow
Observing Cells on the Microscope
Medium Exchange
Medium Exchange with Left-Over Conditioned Medium (Option 1)
Complete Medium Exchange (Option 2)
Installation of Special Setups
Working with Two or more Fluidic Units
Installation of Two or more Fluidic Units
Flow Calibration of Two or more Fluidic Units
Calibration of the Flow Rate with Several Slides in Serial Connection
Oscillatory Flow Experiments
Setting up Oscillatory Flow with Two Fluidic Units
Settings Within the Pumpcontrol Software
Oscillatory Experiment with Four Fluidic Units
Pulsatile Flow Experiments
Technical Details
Working Principle of the Ibidi Pump
Positive Versus Negative Air Pressure
Flow Characteristics
Viscosity
Shear Stress Calculations in Ibidi Channel Slides
Working with Non-Implemented Flow Channels
Maintenance
Disinfection and Cleaning
Silica Beads from the Drying Bottle
Replacement Filters for Perfusion Sets
Fluidic Unit Filters
Fluidic Unit Pinch Valves
Troubleshooting
Air Bubbles
Air Bubbles Emerging When Connecting the Slide
Air Bubbles Emerging after a few Hours
Cells Are Detaching
Cells Detach before Starting the Flow
Cells Detach When Connecting the Slide to the Perfusion Set
Cells Detach under Flow Conditions
Imbalanced Medium
Medium Runs in One Direction Only
No Connection between Ibidi Pump and Pumpcontrol Software
Pressure Kickback after Pressure Switch off
Flow Rate Is too Low or Absent
Flow Rate Is too High
Total Medium Volume in the Perfusion Set Is Decreasing
Flow Direction in the Channel Is Changing
Pressure Lost Error

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Instruction Manual

ibidi Pump System

Version 2.6

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Ibidi pump system quad1090210906