HEKA Elektronik EPC 10 USB Manuals

HEKA Elektronik EPC 10 USB Manuals

Manuals and User Guides for HEKA Elektronik EPC 10 USB. We have 1 HEKA Elektronik EPC 10 USB manual available for free PDF download: Hardware Manual

HEKA Elektronik EPC 10 USB Hardware Manual

HEKA Elektronik EPC 10 USB Hardware Manual (81 pages)

Computer-controlled Patch Clamp Amplifier

Brand: HEKA Elektronik | Category: Amplifier | Size: 11 MB

Table of Contents

Table of Contents
3
1 Introduction

7
- Introducing the EPC 10 USB
  7
- References
  8
- Naming Conventions
  9
2 Description of the Hardware

11
- Headstage
  11
- Red Star Headstage
  11
- Main Unit
  12
  - Front-Panel
    12
  - Rear-Panel
    14
- EPC 10 USB DOUBLE, TRIPLE, and QUADRO
  14
- Special Features of the EPC 10 USB
  15
  - Timing of the AD-/DA-Channels
    15
3 Installation

17
- Installing Software Only
  17
- Installing Hardware and Software
  17
- Conventions
  17
- Connecting the Hardware
  18
- Protection Key ('Dongle') Installation
  18
  - USB Dongle Installation
    18
    
    MS Windows
    19
    
    Macos
    19
- Software Installation
  19
- Calibrating the Hardware
  20
- Support Hotline
  21
4 Verifying and Testing the EPC 10 USB

23
- Testing the EPC 10 USB with the Model Circuit
  23
- The Model Circuit
  23
5 The Control Software

33
- EPC 10 USB Window
  33
  - Main Controls
    33
  - Hidden Controls
    40
- EPC 10 USB Menu
  43
  - Test and Calibrate Menu
    43
6 Operating Modes

45
- Voltage Clamp Mode
  45
- Current Clamp Mode
  45
- Low Frequency Voltage Clamp Mode
  46
- Search Mode
  46
7 Compensation Procedures

49
8 Patch Clamp Setup

55
- Mounting the Probe
  55
- Ground Wires
  55
- Grounding the Microscope
  55
- External Shielding
  55
- Connections to Other Instruments
  56
- Pipette Holder and Electrode
  56
- Bath Electrode
  56
9 Patch Pipettes

59
- Glass Capillaries
  59
- Pulling
  59
- Coating
  60
- Heat Polishing
  60
- Use of Pipettes
  60
10 Using the Patch Clamp

61
- Forming a Seal
  61
- Initial Setup
  61
- Entering the Bath
  61
  - Forming a Gigaseal
    61
  - Cell-Attached Recording
    61
  - Whole-Cell Recording
    62
    
    Breaking the Patch
    62
    
    Capacitive Transient Cancellation
    62
    
    Series Resistance Compensation
    63
  - Current Clamp Recording
    63
11 Low Noise Recording

65
12 Appendix I: Digital Inputs/Outputs

67
- Digital I/O Connector
  67
- Digital in Connector
  68
- Digital out Connector
  68
- Breakout Cable
  69
- Connecting Cables
  69
  - DG 4/5 / Lambda 10-2 / Lambda 10-3 to EPC 10 USB
    69
  - Valvelink 8 to EPC 10 USB
    71
13 Appendix II: Probe Adapters

73
- Standard Mounting Plate
  73
- Dovetail Mounting Plate
  73
14 Appendix III: S-Probe

75
15 Appendix IV: Pipette Holder

77
- BNC-Type
  77
- SMA-Type
  77

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