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Cedex HiRes Cell Analyzer Manuals
Manuals and User Guides for Cedex HiRes Cell Analyzer. We have
1
Cedex HiRes Cell Analyzer manual available for free PDF download: Operator's Manual
Cedex HiRes Operator's Manual (218 pages)
Brand:
Cedex
| Category:
Measuring Instruments
| Size: 8 MB
Table of Contents
Prologue
5
Table of Contents
5
Copyright
7
Document Information
7
Edition Notice
7
Prologue
7
Revision History
7
Trademarks
7
Contact Addresses
8
Declaration of Conformity
8
Intended Use
8
Preamble
8
Conventions Used in this Guide
9
Warnings and Precautions
10
A Startup
12
Turning on the Cedex Hires Analyzer, Control Unit, and Software
13
Preparing the Cedex Hires Analyzer for Measurements
14
Working with the Multi Sampler
14
Installing a Reagent Kit
15
Perform a Prime
16
B Main Application: Counting Cells and Determining Viability
17
Check the Analyzer before Starting Measurements
17
Prepare the Samples
17
Setting up a Single Measurement in the Measurement Dialog Box
18
Open the Measurement Dialog Box and Define the Sample Parameters
18
Define the Measurement and Analysis Parameters
19
Starting the Measurement
20
End of Measurement
21
Viewing the Results in the Measurement Dialog Box
22
Viewing the Results in the Histograms
24
Viewing the Images
24
Setting up Multiple Measurements Using the Multi Run Dialog Box
25
Open the Multi Run Dialog Box and Prepare Samples for Analysis
25
Define the Sample Parameters and the Measurement and Analysis Parameters in the Multi Run Dialog Box
26
Schedule Samples and Start the Measurement
27
Interrupting a Multi Run
28
Results
28
The Multi Run Result List
29
Measurement List
30
C Additional Applications
31
Monitoring Growth of Cells over Time
31
Performing Measurements for Growth Curve Analysis
31
Result Analysis
31
Create a CTC View
32
Add One or more Series of Measurements to the CTC View
33
Create a CTC Chart Associated with the View to Analyze Pattern of Growth over Time
36
Exponential Regression Function to Determine the Specific Growth Rate and Doubling Time of the Cell Culture
38
Checking Regression Curve Fitting
41
Editing Measurements in a View
42
Analyzing Unusual Cells
43
Overview
43
Example of Cells Not Recognized by the Cell Type Std. Size
44
Create a New Cell Type
45
Modify the Parameters for the New Cell Type Via the Live Operator Dialog Box
47
Reprocess the Measurement Using the New Cell Type
50
D Shutting down the Cedex Hires Analyzer
52
Log out
52
Perform an LM Shutdown
52
Shut down the Cedex Control Center
53
Prologue 7
67
Document Information
71
Revision History
71
Edition Notice
71
Copyright
71
Trademarks
71
Prologue
71
Contact Addresses
72
Declaration of Conformity
72
Intended Use
72
Warranty
72
Software Disclaimer
73
Software License Agreement
73
VIII. Preamble
76
Contents of this Operator's Guide
76
Conventions Used in this Guide
77
Warnings and Precautions
78
Disposal of the Analyzer
82
A Overview
83
Description of the Cedex Hires Analyzer
84
The Measurement Procedure
85
Data Analysis
86
Technical Specifications of the Analyzer
87
B System Description
89
Installing the Cedex Hires Analyzer
89
Choosing a Suitable Location
90
Connecting Hardware Components
90
Connecting or Disconnecting the Device
91
Starting the Cedex Hires Analyzer
92
Accessing the Control Unit and Cedex Hires Software
93
Access Control at the Windows Operating System Level
93
Access Control at the Cedex Hires Software Level
94
C Software
95
Overview of the Cedex Control Center
95
Menu Bar
95
System Menu
95
Measurements Menu
96
Functions Menu
96
Reports Menu
97
Master Data Menu
97
Setup Menu
97
Help Menu
98
Shortcut Area
98
Tracking of Status of Events Carried out by the Analyzer
98
The Measurement Dialog Box
99
Preparing Samples for Measurement
99
Setting up a Single Measurement in the Measurement Dialog Box
99
Interrupting a Measurement
100
End of Measurement
101
The Multi Run Dialog Box
102
Setting up Multiple Measurements Using the Multi Run Dialog Box
102
The Status Column
103
Disruption of Measurements
104
Nonlinear Measurements in a Sample Series
104
Using the Load Data from XML Option
105
Results Displayed in the Measurement Dialog Box
107
Result Data Area
108
Sample Parameters and Processing Parameters Areas
109
Image Area
110
Viewing Individual Cell Images
110
Viewing an Image Using the Image View Dialog Box
111
Excluding Images from the Evaluation Process/Including Excluded Images
112
Viewing the Prescan Image
113
Using the Measurement List
114
Viewing Analysis Results
115
Searching for Measurements
115
Editing Existing Analysis Results/Data Sets
116
Reprocessing Measurements
116
Effective Data Sets
117
Graphical Display of the Analysis Results
118
Enlarging the Histograms
118
Aggregate Histogram
119
Cell Diameter Histogram
121
Object Diameter Histogram
122
Defining Diameter Intervals in the Diameter Histograms
122
Compactness Histogram
123
Measurement Statistics Histogram
124
Cultivation Time Chart
125
The CTC View Dialog Box
125
The View Area
126
The Series of Measurements Belonging to Selected View Area
126
Editing Measurements in a View
127
Viewing List of Measurements in a Series
127
Deleting Views and Series
127
Exporting a View
127
Software
127
The Cultivation Time Chart Dialog Box
128
The Data Selection Area
128
Fitting to the Lowest Common Value
129
Measurement Series Offset
129
Variable Axis Calibration
130
The Cultivation Time Chart - Exponential Regression Dialog Box
130
General Functions of the System
131
Data Management
131
Printing Reports
131
Export to File
132
Data Exchange
133
Export
133
Import
135
Viewing and Evaluating the Audit Trail
136
User Preferences
137
Adjusting Result Display in the Measurement List and Multi Run Dialog Boxes
137
Specifying Default Measurement Parameters
138
Specifying Other Default Parameters
138
Specifying the Workarea for the Analysis Data
138
Changing Own Password
139
Administrator Functions
140
Administration of User Accounts
140
Creating User Accounts
140
Creating and Editing User Roles with Specific Access Rights
141
Assigning a User Role to a User
144
Creating User Groups
145
Editing and Deleting User Accounts
145
Password Management
146
Specifying Password Characteristics
146
Assigning New Passwords
146
Assigning Workareas
147
The System Options
148
Modifying the Standard Setting for the Multi Sampler Defaultcup
148
Turning off Automatic Incrementation of Sample Cup Position
149
Turning off the Flow Chamber Prescan
149
Error Level Displayed to Users
150
Reagent Container Configuration
150
Disabling the Reagent Control
150
Changing the Exponent
150
Default Settings
150
Adjustment of the Image Analysis Using the Live Operator
151
Accessing the Cell Type List and Live Operator
152
The Cell Type List Dialog Box
152
Creating a New Cell Type
153
Editing an Existing Cell Type
154
Viewing an Existing Cell Type
155
Deleting an Existing Cell Type
155
Working with the Live Operator
155
Description of the Individual Operator Parameters
157
Archiving Data
159
Archive
160
Restore
162
Deleting Archived Data
163
Database Management
164
Database Backup
164
Database Restore
165
Instrument Configuration
166
Instrument Configuration - Remote Control Area
167
Instrument Configuration - Liquid Management System Area
168
D Maintenance and Care
169
Cleaning and Maintenance Checklist
170
The Multi Sampler Defaultcup
171
The Hardware Management Dialog Box
172
Installing or Changing the Reagent Kit
174
Installing a Reagent Kit
174
Refilling Containers Individually
175
Scheduling Automatic Cleaning Routines
177
Daily Maintenance and Cleaning Routines
178
Cleaning Options
178
Performing a Cleaning Routine
180
Interrupting/Terminating a Cleaning Routine
180
The Prescan Function
180
Scanning the Chamber to Verify the Effectiveness of Cleaning Routines
181
Automatic Adjustment and Alignment Verification
182
Syringe Maintenance
184
Removing the Syringe
184
Assembling and Installing the Syringe
185
Maintenance of the Syringe Guide and Spindle
186
Exchanging the Fuse in Cedex Hires Analyzers from Serial Number G037DA101
187
Maintenance of the Flow Chamber, 8-Way Valve and the Remaining Hardware Components
188
Operational Data
188
The System Suitability Test (SST)
189
Creating SST Checkpoints
189
Creating SST Checkpoints with and Without Measurements
190
Running a Water Blank
191
Setting up the Sstfailurereaction in the System Options Menu
191
Performing an SST
192
Performing an SST with Measurements
192
Performing an SST Without Measurements
193
Tracking Trends in SST Results over Time
194
Tracking Trends in SST Checkpoints with Measurements
194
Tracking SST Checkpoints Without Measurements
196
Locking - Unlocking the System
197
Diagnosis
199
E Troubleshooting
199
The System Audit Trail
200
Troubleshooting
201
F Appendix
203
System Options and User Preferences
203
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