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Isolera Four
Biotage Isolera Four Manuals
Manuals and User Guides for Biotage Isolera Four. We have
1
Biotage Isolera Four manual available for free PDF download: User Manual
Biotage Isolera Four User Manual (116 pages)
Brand:
Biotage
| Category:
Laboratory Equipment
| Size: 5 MB
Table of Contents
Table of Contents
3
1 : Description and Specifications
7
General System Description
7
Basic System Components
8
System Configurations
8
Programmed Functions
9
User Documentation
9
Accessories
9
Software Description
13
Main Menu
13
Data Administration Mode
13
System Mode
14
Chemistry Mode
15
Method Tab (Chemistry Mode)
17
Status Tab (Chemistry Mode)
17
Results Tab (Chemistry Mode)
20
Solvents Tab (Chemistry Mode)
21
Isolera Remote Viewer
22
Specifications
23
General System Specifications
23
Operational Performance Specifications
24
Solvent Specifications
25
Chemical Resistance of the Peristaltic Pump Tube (Isolera LS)
26
2 : Data Administration
27
Log into the Data Administration Mode
27
Administrate the Cartridge List
28
Add a Cartridge Type
28
Delete an Unused Cartridge Type
28
Edit a Cartridge Type
29
Select Cartridge Types to List
29
Administrate the Method List
29
Delete Methods
29
Export Methods
30
Import Methods
30
Set Default Methods
30
Administrate the Rack List
31
Add a Rack Type
31
Delete an Unused Rack Type
32
Edit a Rack Type
32
Select Rack Types to List
32
Administrate the Result List
32
Export and Delete Result Records
33
Clean up the System's Database
33
Administrate the Solvent List
34
Add a Solvent
34
Delete an Unused Solvent
34
Edit a Solvent
35
Select Solvents to List
35
Administrate the User List
35
Add a User Account
35
Change the Password or Privilege
36
Delete a User Account
36
Reset the Number of Performed Runs
36
3 : System Settings
37
Log into the System Mode
37
Change Detector Settings
38
Enable or Disable the Internal Detector
38
Connect and Enable an External Detector
38
Disable an External Detector
39
Enable or Disable the Leak Detector
39
Change General Settings
40
Set the Date and Time
40
Enable or Disable Mouse Pointer
40
Set the Pressure Unit
40
Set the Language Used in the Chemistry Mode
40
Enable the Λ-All Detection Mode and TLC to Step Gradient Editor
41
Configure a Network Connection
41
Change Report Settings
42
Set up a Printer and Auto Print of Reports
42
Set up File Sharing and Auto Save of Reports
43
Set up Auto Send of Reports
44
Change Runtime Settings
44
Enable or Disable Audible Alarm
44
Enable or Disable Automatic Rack Allocation
44
Enable or Disable Peak Mode
45
Enable or Disable Auto Extend
45
Enable or Disable Monitoring of Reservoirs
45
Enable or Disable Run Requirement Estimation
46
Specify How Flushes Are Performed
46
Configure Collection and Fractionation
47
Maintenance
47
Export the System Configuration and Logs
47
Restore the System Configuration
47
Back up and Restore the System's Database
48
Calibrate the Touch Screen
48
Calibrate the Fraction Collector
49
Calibrate the Internal Detector
49
Perform an Intensity Scan of the Internal Detector
49
Clean the Collect Valve
49
Release Stuck Check Valves
50
View and Reset the Usage Statistics
50
4 : Operation
51
Start up the System
51
Shut down the System
51
Control the UV Lamp
52
Create, Open, and Edit Methods
52
Create or Open a Method
52
Calculate Gradient from TLC Data
60
Create a Method Using the Method Wizard
63
Gradient Optimization
63
Create a Method Using a Web Browser
64
Prepare and Run a Purification
65
Prepare the System for a Run
65
Run a Purification
69
Change the Processing Order
72
Monitor and Control a Purification
73
Monitor the Purification in Progress
73
Bypass the Automatic UV Lamp Warm-Up Period
73
End Initial Waste and Start Collecting Fractions
73
Collect through the Waste Channel
74
Start and End an Isocratic Segment
74
Control a Manual Collection
74
Edit and Manually Extend a Purification
74
Pause, End, or Abort a Purification
75
Unload a Purification
76
Flush a Cartridge with Air
76
Purge a Cartridge
77
Access Result Records
78
Search for Records
78
View, Print, and Save Reports on a USB Memory Device or the Network
79
Save Records on a USB Memory Device or the Network
81
View System Status and Results from Your Office
81
Solvent and Waste Handling
82
Assign Solvents to the Solvent Inlets
82
Set the Reservoir Volumes
82
Prime the System
83
Access the Isolera Remote Viewer
85
5 : Maintenance
86
Contact Biotage 1-Point Support
86
Accessories
86
All Isolera Systems
86
Isolera Prime, Isolera One, and Isolera Four
87
Isolera LS
87
Spare Parts
88
Clean the Exterior of the System
88
Implement a Mobile Phase Change
88
Clean the Detector Flow Cell
88
Clean or Release Valves
89
Clean the Pump Check Valves
89
Release Stuck Check Valves
90
Clean the Collect Valve
90
Clean or Replace the Sample Loading Pump Tubing
90
Clean the Sample Loading Pump Tubing
90
Replace the Sample Loading Pump Tubing
91
Sonicate Solvent Inlet Filters
91
Leaks
92
Shut down the System
92
Internal Leakage
92
External Leakage
93
Assembling Tubes Correctly
94
Replace the Fuses
95
Replace the Needle
95
Replace the Detector Lamp(S)
96
6 : Troubleshooting
98
Contact Biotage 1-Point Support
98
Accessories and Spare Parts
98
Fraction Collector-Related Problems
98
Pump-Related Problems
99
Internal Detector-Related Problems
100
Gradient Problems
101
Leak Detected
102
Overpressure Detected
102
7 Manufacturer
103
Biotage Sweden AB
103
Sales Offices and Distributors
103
Technical Support
103
A: Collection and Fractionation
104
Collection and Fractionation Methods
104
Collection Methods
104
Fractionation Methods
104
Detector Signals
104
Possible Combinations
105
Collection and Fractionation Parameters
106
Shoulder Slope and Valley Fractionation
108
Fractionation Using the Shoulder Slope Parameter
108
Fractionation Using the Valley Slope Parameter
110
Collection and Fractionation Examples
111
Threshold Collection
111
Slope Collection
111
Slope Collection and Threshold Fractionation
112
Valley Fractionation
112
Shoulder Slope Fractionation
113
Auto Extend of Gradient
113
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