Axon Axopatch 200B Manuals

Manuals and User Guides for Axon Axopatch 200B. We have 1 Axon Axopatch 200B manual available for free PDF download: Theory Of Operation

Axon Axopatch 200B Theory Of Operation

Axon Axopatch 200B Theory Of Operation (153 pages)

Patch Clamp

Brand: Axon | Category: Industrial Equipment | Size: 0 MB

Table of Contents

Table of Contents
11
List of Figures and Tables
15
Chapter 1: Introduction

17
Chapter 2: Functional Checkout

19
Chapter 3: Use of the Patch Clamp - a Tutorial

27
Chapter 4: Interfacing a Computer to the Axopatch 200B

57
Chapter 5: Low Noise Recording Techniques

59
- Glass Type and Coating
  60
- Seal
  63
- Signal Generator
  64
Chapter 6: Reference Section: Instrument Operation

65
- Bridge Mode
  65
- Capacitance Measurements
  65
- Command Potentials
  66
- Holding Command
  66
- Seal Test
  67
- Current Clamp
  67
- Membrane Potential
  67
- Whole-Cell Current Clamp
  67
- Whole-Cell Parameters
  68
- Electrode Capacitance Compensation
  68
- Limitations
  69
- Electrochemistry
  70
- Headstage
  70
- Offset Adjustment for Headstage
  70
- Frequency Boosting
  71
- Case Ground Connector
  71
- Mounting the Headstage
  71
- Cleaning
  71
- Static Precautions
  72
- Optical Pick-Up
  72
- Acoustic Pick-Up
  72
- Tuning the Headstage
  72
- Holders
  73
- Features
  73
- Parts
  74
- Use
  75
- Glass Dimensions
  77
- Adapters
  77
- Input Bncs
  77
- Output Section
  79
- Filter
  79
- Output Gain (Α Α Α Α )
  79
- Output Bncs
  80
- Panel Meter
  83
- Power-Supply Voltage Selection and Fuse Changing
  84
- Supply Voltage
  84
- Changing the Fuse
  84
- Probe Temperature
  84
- Zap
  85
Chapter 7: Reference Section: General Information

87
- Grounding and Hum
  87
- Model Cell
  88
- Model Bilayer
  89
- Power-Supply Glitches
  90
- Ten-Turn Potentiometers
  90
Chapter 8: Reference Section: Principles of Operation

91
- Headstages
  91
- Principals of Operation
  91
- Resistor Feedback
  91
- Capacitor Feedback
  92
- If the Probe Output Is Slow, How Can Voltage Clamping be Fast
  94
- What Is Clamped During Voltage Clamping
  95
- Capacitance Compensation
  95
- Pipette Capacitance Compensation
  95
- Whole-Cell Capacitance Compensation
  97
- Absolute Value
  99
- Limitations
  99
- Series Resistance
  99
- Correction, Prediction, and Lag
  99
- Saturation Effects
  103
- Oscillations
  106
- Using Lag to Prevent Oscillations
  106
- Limitations
  106
- Pipette Offset
  107
- Leak Subtraction
  108
- Zap
  109
- Lipid Bilayers
  109
- Experimental Techniques
  109
- Noise Vs. Access Resistance
  111
- Current and Voltage Conventions
  112
- Troubleshooting
  117
Chapter 9: SPECIFICATIONS

119
Chapter 10: REFERENCES

135
Chapter 11: HEADSTAGE TUNING PROCEDURE

137
- Index
  143
- Warranty
  145
- Warning
  147
- Circuit Diagrams Request
  149

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