Sign In
Upload
Manuals
Brands
Axon Manuals
Industrial Equipment
Axopatch 200B
Axon Axopatch 200B Manuals
Manuals and User Guides for Axon Axopatch 200B. We have
1
Axon Axopatch 200B manual available for free PDF download: Theory Of Operation
Axon Axopatch 200B Theory Of Operation (153 pages)
Patch Clamp
Brand:
Axon
| Category:
Industrial Equipment
| Size: 0 MB
Table of Contents
Table of Contents
11
List of Figures and Tables
15
Chapter 1: Introduction
17
Chapter 2: Functional Checkout
19
Chapter 3: Use of the Patch Clamp - a Tutorial
27
Single Channel Recording (Model Cell)
28
Pipette Offset Adjustment
28
Using the TRACK Mode
28
Using the V-CLAMP Mode
29
Adjustment of Pipette Capacitance Compensation
30
Adjustment of Leak Subtraction
30
Whole-Cell Recording (Model Cell)
31
Pipette Offset Adjustment
32
Whole-Cell Capacitance Compensation
32
Series Resistance Compensation
35
Brief Method for Setting Series Resistance Compensation
35
Detailed Method for Setting Series Resistance Compensation
38
Current Clamp (Model Cell)
44
Single-Channel Recording (Real Cell)
46
Whole-Cell Recording (Real Cell)
51
Chapter 4: Interfacing a Computer to the Axopatch 200B
57
Chapter 5: Low Noise Recording Techniques
59
Glass Type and Coating
60
Seal
63
Signal Generator
64
Chapter 6: Reference Section: Instrument Operation
65
Bridge Mode
65
Capacitance Measurements
65
Command Potentials
66
Holding Command
66
Seal Test
67
Current Clamp
67
Membrane Potential
67
Whole-Cell Current Clamp
67
Whole-Cell Parameters
68
Electrode Capacitance Compensation
68
Limitations
69
Electrochemistry
70
Headstage
70
Offset Adjustment for Headstage
70
Frequency Boosting
71
Case Ground Connector
71
Mounting the Headstage
71
Cleaning
71
Static Precautions
72
Optical Pick-Up
72
Acoustic Pick-Up
72
Tuning the Headstage
72
Holders
73
Features
73
Parts
74
Use
75
Glass Dimensions
77
Adapters
77
Input Bncs
77
Output Section
79
Filter
79
Output Gain (Α Α Α Α )
79
Output Bncs
80
Panel Meter
83
Power-Supply Voltage Selection and Fuse Changing
84
Supply Voltage
84
Changing the Fuse
84
Probe Temperature
84
Zap
85
Chapter 7: Reference Section: General Information
87
Grounding and Hum
87
Model Cell
88
Model Bilayer
89
Power-Supply Glitches
90
Ten-Turn Potentiometers
90
Chapter 8: Reference Section: Principles of Operation
91
Headstages
91
Principals of Operation
91
Resistor Feedback
91
Capacitor Feedback
92
If the Probe Output Is Slow, How Can Voltage Clamping be Fast
94
What Is Clamped During Voltage Clamping
95
Capacitance Compensation
95
Pipette Capacitance Compensation
95
Whole-Cell Capacitance Compensation
97
Absolute Value
99
Limitations
99
Series Resistance
99
Correction, Prediction, and Lag
99
Saturation Effects
103
Oscillations
106
Using Lag to Prevent Oscillations
106
Limitations
106
Pipette Offset
107
Leak Subtraction
108
Zap
109
Lipid Bilayers
109
Experimental Techniques
109
Noise Vs. Access Resistance
111
Current and Voltage Conventions
112
Troubleshooting
117
Chapter 9: SPECIFICATIONS
119
Chapter 10: REFERENCES
135
Chapter 11: HEADSTAGE TUNING PROCEDURE
137
Index
143
Warranty
145
Warning
147
Circuit Diagrams Request
149
Advertisement
Advertisement
Related Products
Axon Synapse 2AS12
Axon Synapse 2GM100
Axon Synapse 2HM100
Axon Synapse 2IX09
Axon Synapse TWINS 2AS11
Axon TASER StrikeLight 2 Stun
Axon Body 2 AX1001
Axon Body 2 AX1019
Axon Axopatch-1D
Axon EZ-24 BR
Axon Categories
Digital Camera
Amplifier
Media Converter
Docking Station
Dash Cameras
More Axon Manuals
Login
Sign In
OR
Sign in with Facebook
Sign in with Google
Upload manual
Upload from disk
Upload from URL