LI-COR Odyssey Operator's Manual
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LI-COR Odyssey Operator's Manual

Clx infrared imaging system
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Notes on Safety
LI-COR products have been designed to be safe when operated in the manner described in this manual.
The safety of this product can not be guaranteed if the product is used in any other way than is specified
in this manual. The Odyssey Fc Imager is intended to be used by qualified personnel. Read this entire
manual before using the Odyssey Fc Imager.
Equipment Markings:
The product is marked with this symbol when it is necessary for you to
refer to the manual or accompanying documents in order to protect
against damage to the product.
The product is marked with this symbol when a hazardous voltage
may be present.
Manual Markings:
WARNING
Warnings must be followed carefully to avoid bodily injury.
CAUTION
Cautions must be observed to avoid damaging your equipment.
NOTE
Notes contain additional information and useful tips.
IMPORTANT
equipment or related software. Failure to comply may result in a poor experimental
outcome but will not cause bodily injury or equipment damage.
Operator's
Manual
®

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Summary of Contents for LI-COR Odyssey

  • Page 1 The safety of this product can not be guaranteed if the product is used in any other way than is specified in this manual. The Odyssey Fc Imager is intended to be used by qualified personnel. Read this entire manual before using the Odyssey Fc Imager.
  • Page 2 CE Marking: This product (model number 2800) is a CE-marked product. For conformity information, contact LI-COR Support at biosupport.licor.com. Outside of the U.S., contact your local sales office or distributor.
  • Page 3 LI-COR products have been designed to be safe when operated in the manner described in this manual. The safety of this product can not be guaranteed if the product is used in any other way than is specified in this manual. The Odyssey ®...
  • Page 4 LI-COR is an ISO 9001 registered company. © 2011 LI-COR, Inc. All rights reserved. Specifications subject to change. LI-COR, Odyssey, MPX, FieldBrite, and IRDye are trademarks or registered trademarks of LI-COR Inc. in the United States and other countries. The Odyssey Fc Imaging System, FieldBrite XT technology,...
  • Page 5 PRODUCT, you agree to be bound by the terms of this EULA. If you do not agree to the terms of this EULA, do not install or use the SOFTWARE PRODUCT; you may, however, return it to LI-COR, Inc. for a full refund.
  • Page 6 Support Services shall be considered part of the SOFTWARE PRODUCT and is subject to the terms and conditions of this Agreement. With respect to technical information you provide to LI-COR as part of the Support Services, LI-COR may use such information for its business purposes, including for product support and development.
  • Page 7 3. UPGRADES/ENHANCEMENTS. If the SOFTWARE PRODUCT is labeled as an upgrade, you must be properly licensed to use a product identified by LI-COR as being eligible for the upgrade in order to use the SOFTWARE PRODUCT. A SOFTWARE PRODUCT labeled as an upgrade replaces and/or supplements the product that formed the basis for your eligibility for the upgrade.
  • Page 8 AGREEMENT, LI-COR'S ENTIRE LIABILITY REGARDING SUPPORT SERVICES SHALL BE GOVERNED BY THE TERMS OF THAT AGREEMENT. 6.4. Exclusive Remedy: TO THE EXTENT THAT THE LICENSOR IS LIABLE, THE EXCLUSIVE REMEDY, AT LI-COR'S OPTION SHALL BE EITHER (A) RETURN OF THE PRICE PAID, IF ANY, OR (B) REPAIR OR REPLACEMENT OF THE LICENSED PROGRAM THAT DOES NOT MEET LI-COR'S LIMITED WARRANTY AND WHICH IS RETURNED TO LI-COR WITH A COPY OF PROOF OF PURCHASE.
  • Page 9 7.8. All communications made in connection with the alternative dispute resolution procedure set forth in this Section shall be treated as communications for the purpose of settlement and as such shall be deemed to be confidential and inadmissible in any subsequent litigation by virtue of Rule 408 of the Federal Rules of Evidence, as the same may be amended from time-to-time.
  • Page 10 LI-COR’s or its licensors’ names or any of their respective trademarks. LI-COR is an ISO9001 registered company. ©2011 LI-COR, Inc. All rights reserved. Specifications subject to change. LI-COR, Odyssey, MPX, and IRDye are trademarks or registered trademarks of LI-COR, Inc.

  • Page 11: Table Of Contents

    Table of Contents Chapter 1: Installation and Safety Information Safety Considerations ................1 Laser Safety .
  • Page 12 Chapter 3: Maintenance Routine Maintenance ................13 Cleaning the Fan Filter.

  • Page 13: Chapter 1: Installation And Safety Information

    Fc Imager is certified as a Class I laser product. This means that hazardous laser radiation is not emitted outside the instrument. Radiation emitted inside the Odyssey Fc is confined within protective housings and external cov- ers. A series of safety interlocks ensures that the laser beam cannot escape during any phase of user operation.

  • Page 14: Safety Interlocks

    Figure 1-4. Location of class 4 laser warning label located on (a) the camera hood under the top panels of the Odyssey Fc instrument enclosure and (b) on the inner front access panel (do not open, no user-serviceable parts inside).

  • Page 15: Placement In The Laboratory

    Instrument Ventilation The air intake fan on the back of the Odyssey Fc instrument (Figure 2-4) is used to cool the internal electronics and maintain fresh air circulation throughout the internal volume of the instrument. The air intake is filtered and the filter should be periodically cleaned (Chapter 3).

  • Page 16: Electrical Considerations

    Do not defeat the purpose of the grounding-type plug. Do not locate the Odyssey Fc Imager where the power cord will be walked on or exposed to water or chemical spills. The Odyssey Fc Imager draws a maximum of 2 amperes at 120V (see Chapter 4 for complete specifications). If an extension cord is used, make sure the total of the ampere ratings on the instruments plugged into the extension cord does not exceed the extension cord ampere rating.

  • Page 17: Chapter 2: System Overview

    Imaging tray storage shelf ® Figure 2-1. Front panel features and controls of the Odyssey Fc Imager. Power On/Off Switch: Pressing this switch momentarily turns the instrument on and off. Pressing the switch for 5 seconds or more cuts power to the instrument (Chapter 3). While the instrument is performing its startup or shutdown procedure, the blue indicator light in the power switch blinks.
  • Page 18 Chapter 2: System Overview instrument is ready for operation. Pressing the Power On/Off button when the green image acquisition indicator is lit will cause the red error condition indicator to illuminate. This is just a warning that the instrument is in use. Green Image Acquisition Indicator: This indicator light blinks during image acquisition and is continuously on at other times, as long as communication with the Image Studio software is maintained.

  • Page 19: Right-Side And Rear Panels

    Keep the intake unblocked during operation and accessible for routine filter cleaning (Chapter 3). ® Figure 2-3. Close up of the Odyssey Fc control module showing the power receptacle and ethernet connectors (use only the supplied ethernet cable). All other connectors on this panel should not be used.

  • Page 20: Imaging Drawer

    During an unexpected power loss, the drawer button will not function. This may leave a sample inside the Odyssey Fc Imager, or it may result in the imaging drawer being left partially open or closed. If the power remains off, the drawer can be opened or closed by pulling or pushing on the drawer front.

  • Page 21: Sample Placement

    For Western blotting methods, nitrocellulose or PVDF membranes may be used (see www.licor.com/bio for the latest membranes and kits that are optimal for infrared detection). Detailed blotting protocols can be found in the Odyssey Fc Application Protocols Manual. There are some general tips, however, for using membranes.

  • Page 22: Using Gels

    Image acquisition is started via Image Studio software. The Odyssey Fc has four detection channels: two are infrared channels with 685-690 and 785 nm lasers, one is a 600 nm channel with a light source for excitation, and the fourth is for detecting visible wavelength emissions from chemilumi- nescent substrates.

  • Page 23: Computer Connections And Networking

    Odyssey Fc Imager Computer Connections and Networking Note: Use only the supplied Cat. 5 RJ45 ethernet cable to connect the Odyssey Fc Imager and computer. The ethernet ® ports on the Odyssey Fc Imager are both Auto-MDIX, so a cross-over cable is not needed.

  • Page 24: Chapter 3: Maintenance

    The air intake fan on the back of the Odyssey Fc instrument is used to cool the internal electronics. Periodically inspect the air intake filter (Figure 3-1). Clean or replace the filter when it looks like dust is starting to block air flow. To test air flow, place a piece of standard printer paper over the filter while the instrument is running.

  • Page 25: Front Panel Warning Light Indicates An Error

    Fc Imager is unable to complete a task requested by Image Studio software, or some hardware fail- ure is encountered, the red error indicator on the Odyssey Fc front panel (Figures 2-1 and 2-2) flashes and an audible alarm sounds. This error condition is automatically cleared after a few seconds and a message dialog is displayed in the Image Studio software to explain the error.
  • Page 26 1) If necessary, turn off the Odyssey Fc Imager by first disconnecting the instrument from Image Studio and then briefly pressing the power switch on the Odyssey Fc front panel. 2) Disconnect the power cord from rear of the instrument.

  • Page 27: Obtaining Technical Support

    Note: If an error message to replace the desiccant is displayed after replacing the desiccant, power off the Odyssey Fc Imager and wait an hour or two. After waiting for the air inside the camera to dry, power on the Odyssey Fc Imager, start the application software, and see if the Status changes to “Ready”...

  • Page 28: Chapter 4: Appendix

    600, 700 and 800 nm channels: 30 s, 2 min, 10 min plus advanced variable time feature. Chemiluminescence channel: 30 s, 2 min, 10 min, 60 min plus advanced variable time feature. Focusing: None needed. ® Sensitivity: Similar to Odyssey Infrared Imaging System Wavelength Maxima for Excitation/Emission Bands: Channel...
  • Page 29 Fc Imager is certified as a Class 1 laser product. Hazardous laser radiation is not emitted outside the instrument. Radiation emitted inside the Odyssey Fc Imager is confined within protective housings and external covers. A series of safety interlocks ensures that laser beams cannot escape during any phase of user operation.

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