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Related Manuals for Photometrics Evolve 128
Summary of Contents for Photometrics Evolve 128
- Page 1 USER MANUAL Evolve 128...
- Page 2 User Manual for Evolve 128...
- Page 3 Other brand and product names are the trademarks or registered trademarks of their respective owners and manufacturers. The information in this publication is believed to be accurate as of the publication release date. However, Photometrics does not assume any responsibility for any consequences including any damages resulting from the use thereof. The information contained herein is subject to change without notice.
- Page 4 Customer Service If you have any questions about your camera system, please contact Photometrics Customer Service. When you call, please have your Photometrics part number or equipment serial numbers available. BENELUX FRANCE Photometrics Roper Scientific, BV Roper Scientific, SARL 3440 East Britannia Drive Ir.
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Page 5: Limited Warranty
Photometrics warrants this product against substantial defects in materials and / or workmanship for a period of up to one (1) year after shipment. During this period, Photometrics will repair the product or, at its sole option, repair or replace any defective part without charge to you. - Page 6 Photometrics. 2. You must notify the Photometrics factory service center within (30) days after you have taken delivery of a product or part that you believe to be defective. With the exception of customers who claim a “technical issue”...
- Page 7 Evolve 128 Users Manual...
- Page 8 Evolve 128 Users Manual...
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Page 9: Table Of Contents
Installing the FireWire Interface Card .................. 6 Software Installation ......................... 7 Connecting Your Evolve 128 Camera ..................7 To connect your Evolve 128 camera ..................7 Chapter 3 Operating Features Introduction ..........................9 On-Chip Multiplication Gain ....................9 ... - Page 10 Chapter 5 Basic Specifications Index ..........................33 Figures Figure 1. Comparison of Traditional CCD and Evolve 128 CCD Array Structures ..2 Figure 2. FireWire interface Card ....................2 Figure 3. DATA Cable ......................8 ...
- Page 11 Tables Table 1. Evolve 128 Readout Speeds and Typical Conversion Gains ......17 Table 2. Application Type and Evolve 128 Setup Parameters ......... 18 Table 3. CCD Specifications ....................28 Table 4. Input/Output Definitions ..................30 ...
- Page 12 THIS PAGE INTENTIONALLY LEFT BLANK Evolve 128 Users Manual...
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Page 13: Chapter 1 Overview
Chapter 1 Overview Introduction The Photometrics® Evolve camera employs an advanced CCD technology called "impact ionization" to achieve charge multiplication gain above the read noise. As a result, the cameras offer unparalleled sensitivity even at high frame rates. Evolve cameras are most suited for high speed and/or low-light level imaging applications such as single molecule fluorescence, ion imaging, and adaptive optics. -
Page 14: System Components
Figure 2. FireWire interface Card About This Manual The Evolve 128 User Manual is divided into five chapters. It is suggested that you read the entire manual before operating the camera in order to ensure proper use. The chapter contents are briefly described below. -
Page 15: Precautions
Do not use a C-mount lens with optics that extend behind the lens flange. Environmental Requirements The Evolve 128 camera system should be operated in a clean, dry environment. The camera system’s ambient operating temperature is 0°C to 30°C with 80% relative humidity, non- condensing. -
Page 16: Microscopes, Lenses, And Tripods
The Evolve 128 camera can be mounted to a tripod using the tripod mounting attachment located on the sides of the camera. -
Page 17: Chapter 2 System Installation
Carefully review the Precautions section in the previous chapter before performing any of the procedures outlined here. Again, use only an Evolve data cable and an Evolve FireWire interface card with your Evolve 128 camera. Using a different cable or interface card may result in permanent damage to your system. -
Page 18: Host Computer Requirements
Note: The above requirements are the minimum for operating an Evolve camera. A faster computer with larger memory (RAM) will greatly enhance the software performance during live mode operations. If you are a Mac® user, the host computer for your Evolve 128 camera must have the following: ... -
Page 19: Software Installation
Connector Pinout section in Chapter 5 for details) Power switch: Turns the camera on and off. The following connector is located on the power supply of the Evolve 128 camera: I/O connector: Hirose HR10A-10R-10S, 10-pin connector for input/output... -
Page 20: Figure 3. Data Cable
2. Connect the right-angled end of the DATA cable to the FireWire port (indicated with a red arrow in next graphic) located on the side of the camera Figure 4. Evolve 128 Side Panel 3. Verify that the power switch on the side of the camera is in the off position 4. -
Page 21: Chapter 3 Operating Features
Operating Features Introduction The features described in this section are identical in all Evolve 128 cameras unless otherwise stated. On-Chip Multiplication Gain The Evolve 128 uses a unique CCD capable of multiplying the charge (electrons) generated in the pixels. When the multiplication is sufficiently high, it is possible to see extremely low- light events. -
Page 22: Non-Overlap Mode
“Pre-exposure” with clear count of “1”(or more). Figure. 6 shows these settings on a software dialogue box. Note: Since the software you are using may show the settings differently, you should refer to the software documentation for accurate information. Evolve 128 Users Manual... -
Page 23: Example: Non-Overlap Mode
Referring to figure 7, it can be seen that exposure and readout are carried out in non- overlapped fashion. As a result, each frame in the sequence is precisely exposed for the time specified (i.e. 10 ms). Evolve 128 Users Manual... -
Page 24: Overlap Mode (Simultaneous Exposure-Readout)
This mode of operation allows you to continuously image a specimen to obtain better kinetic information about a process. Figure 8 shows the required Evolve 128 settings for overlap mode. Note that the clearing mode is set to "Pre-sequence" (as opposed to "Pre-exposure" in Non-Overlap mode). -
Page 25: Figure 9. Timing Diagram For Overlap Mode When Exposure Time < Readout Time
= readout time for one frame From the timing diagram, you can see that because the exposure time is greater than the readout time, all frames are precisely exposed for the duration entered into the software and have similar intensities. Evolve 128 Users Manual... -
Page 26: Triggered Operation
Photometrics to access primary signals such as "Trigger input," "Expose out," "Frame readout," and "Shutter out." In the default mode, the camera triggers on the rising edge of a TTL signal. Evolve 128 cameras support the trigger modes described in the next sections. Trigger-first Mode In this mode, the camera requires only one trigger to acquire a sequence of frames. -
Page 27: Strobe Mode
Read Out is completed. It goes low when the Trigger signal is accepted and exposure is started. The shaded areas ( ) denote the idle time between exposures. Strobe mode only operates in Non-Overlap mode. Figure 13. Strobe Mode Timing Diagram Evolve 128 Users Manual... -
Page 28: Bulb Mode
Multiplication Amplifier Operation of multiplication gain in the Evolve 128 is different from the previous generation of these cameras. The new “Evolve” generation of cameras has new functionality that was not available on previous models. A setting of 0 (zero) results in unity gain and the subsequent settings are linearly related to the actual multiplication gain. -
Page 29: Conversion Gain
1, 2, 4, 8, and 16 and asymmetric binning of 1 to 128 in both the serial (horizontal) and parallel (vertical) directions. Note: Due to the small array size in the Evolve 128 edge artifacts may be present at higher binning. Evolve 128 Users Manual... -
Page 30: Evolve 128 Application Examples
Application Settings Summary The table below summarizes typical Evolve 128 settings for two types of applications and is provided as a setup aid. Actual settings will vary based on the exact nature of your experiment design. Readout... -
Page 31: Evolve 128 Features
65,535 gray levels per pixel can be sent over the bus. However, for the CCD60 used in the Evolve 128 camera, the total number of electrons possible in a single pixel can easily reach 200,000. Thus, if the camera was set at 1 electron per gray level, then the pixel would come across as saturated (it would max out at the 65,535 value). -
Page 32: Background
These are often referred to as “speckles” in the image. Photometrics has developed this real-time feature to help users identify such events and correct for them, in their images in real-time. -
Page 33: Vari-Bit
Using higher-bit-depth digitization does not make measurements less quantitative when measuring a scene with a low dynamic range; however, it does introduce many extra (or unnecessary) gray levels that do not represent actual physical photons. Figure 16. Benefit of Varibit Evolve 128 Users Manual... -
Page 34: Rapid-Cal
Rapid-Cal Calibration The Evolve 128 has incorporated a calibration routine which allows the camera to adjust voltages of the ADC offsets such that the electron multiplication gain input given to the camera reflects the actual gain provided by the device. Settings 1 to 1000 on the electron multiplication gain slider are mapped linearly to provide the actual multiplication gain requested by the user. -
Page 35: Black-Lock / Top-Lock
This advanced feature allows users to define an effective floor for an image at a level which they select. In the case of the Evolve 128, many users operate the camera in EM gain mode and apply EM gain at high levels. In such scenarios, EMCCD cameras can end up having multiple ADUs per electron. - Page 36 Utilizing Top-Lock and Black-Lock together allows users to narrow visualization rapidly and effectively into the intensity range of the image features they are most interested in. Black-lock and Top-lock in action For further explanation, including a real-time demonstration of black-lock and top-lock, please visit www.evolve-emccd.com Evolve 128 Users Manual...
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Page 37: Chapter 4 Troubleshooting
If no images appear: Confirm that the camera switch is set to on. Confirm that the Evolve 128 camera is selected in your imaging software application. Power off the camera and the host computer and check all system connections (particularly the DATA and power cables). -
Page 38: Camera Running Too Warm
C-mount lens attached to your Evolve 128. Using normal room lighting, place the camera on a table about 3 meters away from an object and acquire an image. If the problem persists, contact Photometrics Customer Service. Camera Running Too Warm It is normal for the camera to be slightly warm to the touch while in operation. -
Page 39: Chapter 5 Basic Specifications
Chapter 5 Basic Specifications Evolve 128 Front/Side Views Figure 19. Evolve 128 Front View Figure 20. Evolve 128 Side View Evolve 128 Users Manual... -
Page 40: Camera Weight
Digitalization (Readout) 10 MHz Rate Table 3. CCD Specifications Connectors Figure 21. Evolve 128 Side Panel The following connectors are located on the side of the camera. connector: Type 1, 6 pin IEEE-1394a (FireWire). POWER connector: 25-pin, Dsub connector for camera power and I/O functions. -
Page 41: Power Connector Pinout
13. +16V 14. FPGA-GND 15. SPARE IO-2 Figure 22. Power Connector Pinout 16. EXPOSE 17. TX-IO 18. EOF 19. GND 20. TEC GND 21. TEC CONTROL 22. TEC GND 23. + 37V 24. GND 25. GND Evolve 128 Users Manual... -
Page 42: Figure 23. Input/Output Connector
A high level on this output indicates that the camera power is switched on (+5 V = on, 0 V = off). System digital ground. Any external circuitry intended to interface with the trigger control signals must reference this ground connection. Table 4. Input/Output Definitions Evolve 128 Users Manual... -
Page 43: Power Supply Specifications
Power Supply Weight: 5 lb (2.267 kg) Supply Cable Length: 6 ft. / 1.828 m Note: CE certification applies to the Evolve 128 only when the camera system is operated with a CE- approved power supply Figure 24. Power Supply Front... -
Page 44: Figure 25. Liquid Cooled Evolve Setup Diagram
17. Turn on camera power and continue setup per quick-start guide. NOTE: DO NOT turn-on camera power without liquid circulating! 18. Open imaging software to set target temperature (-100C.) 19. Allow approximately 30 minutes to stabilize at target temperature. Figure 25. Liquid Cooled Evolve Setup Diagram Evolve 128 Users Manual... -
Page 45: Index
L T Lenses, 4 Trigger modes bulb, 17 M strobe, 16 Microscopes, 4 trigger-first, 15 Multiplication Trigger-first mode, 15 gain, 1 Tripods, 4 register, 1 W N Warranty, iii Non-overlap readout mode, 11 Your responsibility, iv Evolve 128 Users Manual... - Page 46 57-519-004 Rev A1...
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