Photometrics CoolSNAP cf User Manual

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  • Page 2 User Manual for CoolSNAP , CoolSNAP , CoolSNAP and CoolSNAP Systems...
  • Page 3 Roper Scientific, Inc. Printed in the United States of America. CoolSNAP is a trademark and Photometrics, PVCAM, and Roper Scientific are registered trademarks of Roper Scientific, Inc. Mac, Macintosh, Mac OS, and Panther are trademarks of Apple Computer, Inc., registered in the U.S. and other countries.
  • Page 4 LIMITED WARRANTY — Photometrics Analytical Instrumentation Photometrics, a division of Roper Scientific, Inc. (“Photometrics,” us,” “we,” “our”) makes the following limited warranties. These limited warranties extend to the original purchaser (“You”, “you”, "Your", "your") only and no other purchaser or transferee. We have complete control over all warranties and may alter or terminate any or all warranties at any time we deem necessary.
  • Page 5 Your Responsibility The above Limited Warranties are subject to the following terms and conditions: You must retain your bill of sale (invoice) and present it upon request for service and repairs or provide other proof of purchase satisfactory to Photometrics. You must notify the Photometrics factory service center within (30) days after you have taken delivery of a product or part that you believe to be defective.
  • Page 6 U. S. Government Restricted Rights The software and documentation are provided with Restricted Rights. Use, duplication, or disclosure by the Government is subject to restrictions as set forth in subparagraph (c)(1)(ii) of the Rights in Technical Data and Computer Software clause at DFARS 252.227-7013 or subparagraphs (c)(1) and (2) of the Commercial Computer Software-Restricted Rights at 48 CFR 52.227-19, as applicable.
  • Page 7: Declaration Of Conformity

    Declaration of Conformity Roper Scientific, Inc. declares that the equipment described in this document is a CLASS A DEVICE in conformance with the requirements of the European Council Directives, listed below: 89/336/EEC EMC Directive & Amendments 93/68/EEC EMC Directive & Amendments...
  • Page 8 Declaration of Conformity Roper Scientific, Inc. declares that the equipment described in this document is a CLASS A DEVICE in conformance with the requirements of the European Council Directives, listed below: 89/336/EEC EMC Directive 92/31/EEC Amendment 93/68/EEC Amendment 73/23/EEC Product Safety on the application of the laws of Member States relating to Electromagnetic Compatibility and Product Safety.
  • Page 10: Table Of Contents

    Table of Contents Chapter 1. Introduction Description ............................1 System Components........................1 About This Manual......................... 2 Precautions............................2 Environmental Requirements....................... 2 Storage Requirements ........................3 Microscopes and Lenses ........................ 3 Repairs .............................. 3 Cleaning............................3 Photometrics Customer Service ....................4 Chapter 2.
  • Page 11 Chapter 5. Basic Specifications Dimensions.............................15 CCD Specifications and Orientations ..................17 Additional Measurements ......................17 Detector ............................18 Auxiliary Power Supply ......................19 Usage Requirements......................19 Installation..........................19 Specifications ..........................19 viii...
  • Page 12: Chapter 1. Introduction

    Introduction Chapter 1. Description The CoolSNAP , CoolSNAP , CoolSNAP , and CoolSNAP , from ® Photometrics , are ideal cameras for digital microscopy and many other biological applications. The simple, compact CoolSNAP and CoolSNAP incorporate a high- quality CCD (charge-coupled device), a 12-bit scientific digitizer, and low-noise electronics to produce high-quality 12-bit monochrome or 36-bit digital color images at greater than 1k x 1k resolution.
  • Page 13: About This Manual

    The CoolSNAP User Manual is divided into five chapters. It is suggested that you read the entire manual before operating the camera in order to ensure proper About This use. The chapters that follow this introduction are: Manual System Installation — Instructions for installing the camera system’s •...
  • Page 14: Storage Requirements

    Storage Store the CoolSNAP camera system in its original containers. To protect the system from excessive heat, cold, and moisture, store at an ambient temperature Requirements between 0°C and 70°C with a relative humidity of 0%-80% noncondensing. Microscopes and The camera has a standard threaded video mount and can be mounted to any microscope that accepts a standard C-mount adapter.
  • Page 15: Photometrics Customer Service

    In Europe, you can reach Customer Service at: BENELUX Tel: 31.347.324989 • Fax: 31.347.324979 • Email: mailto@roperscientific.com • Mail: Roper Scientific, BV • Ir. D.S. Tuijnmanweg 10 4131 PN VIANEN, Netherlands FRANCE Tel: 33.160.86.03.65 • Fax: 33.160.86.07.09 • Email: princeton.instruments@wanadoo.fr •...
  • Page 16: Chapter 2. System Installation

    System Installation Chapter 2. Carefully review the Precautions section on page 2 before performing any of the procedures outlined here. Again, use only a CoolSNAP cable and a CoolSNAP interface card with your CoolSNAP camera. Using a different cable or interface card may result in permanent damage to your system.
  • Page 17: Multiple Cameras

    If you are a Mac user, the host computer for your CoolSNAP camera must have the following: Macintosh OS X.3 (Panther) • 512 MB RAM (or greater) • CD-ROM drive • At least one unused PCI or PCI-X card slot •...
  • Page 18: Connecting Your Coolsnap Camera

    Connecting Your The CoolSNAP cable connects your CoolSNAP camera to the interface card. This cable is designed to serve as a conduit both for data and power. CoolSNAP Camera CoolSNAP IEEE-1394 Cable CoolSNAP LVDS Cable To connect your CoolSNAP LVDS camera: Connect either end of the CoolSNAP LVDS cable to the CoolSNAP interface card that you have installed in the host computer.
  • Page 19 To connect your CoolSNAP IEEE-1394 camera: Connect either end of the CoolSNAP IEEE-1394 cable to the CoolSNAP IEEE-1394 interface card that you have installed in the host computer. Connect the other end of the CoolSNAP IEEE-1394 interface cable to either DATA connector located on the back of the camera (shown below).
  • Page 20: Chapter 3. Operating Features

    Operating Features Chapter 3. Common Features The features described in this section are identical in all four CoolSNAP cameras. Features that are specific to the CoolSNAP and CoolSNAP cameras are described separately in the "CoolSNAP / CoolSNAP " section. Readout Speed All four CoolSNAP cameras operate at 20-MHz digitization and offer greater than 10 full frames per second.
  • Page 21: Antiblooming

    If the exposure time is 50 ms, which is less than the readout time, the camera operates in "nonoverlapped" mode (see Figure 2). The effective frame rate for this is 7.14 fps (1/[0.05+0.090]). While in normal mode, the camera firmware automatically calculates the readout times, taking into account binning and/or subregion, and carries out the exposure-readout sequence to maximize the frame rate.
  • Page 22: Application Examples

    Application Examples Example 1 "I have a CoolSNAP (or CoolSNAP ) and want to operate the camera in the most sensitive setting for taking high-resolution, single images." For this application, the camera should be operated in "alternate normal" mode to provide the best quantum efficiency and the camera gain should be set to 2. These settings will operate the camera in its most sensitive mode.
  • Page 23: Chapter 4. Troubleshooting

    Troubleshooting Chapter 4. If you have any difficulty while troubleshooting, or do not see your camera system’s symptoms listed here, contact Photometrics Customer Service. System Does Not If your operating system does not boot normally after you have installed a interface card, try installing the new card in another open interface slot.
  • Page 24: Images Not Displayed Properly

    Images Not If the amber LED on the back of the camera does not illuminate while images are being acquired and no images appear: Displayed Properly Confirm that the green LED on the back of the camera is illuminated, • indicating that the camera is powered on.
  • Page 25: Processor Or Hyperthreading-Capable Pcs

    IEEE-1394 Some high-end Windows 2000/XP-enabled computers with Intel Pentium or ™ Xeon processors include a memory enhancement called Physical Address CoolSNAP Extension (PAE). PAE allows these computers to address memory above 4GB; Camera Will Not however, when PAE is enabled the IEEE-1394 CoolSNAP camera will not Image When transmit any pixel data.
  • Page 26: Chapter 5. Basic Specifications

    Basic Specifications Chapter 5. Dimensions CoolSNAP Camera: Front View CoolSNAP LVDS Camera: Side View...
  • Page 27 CoolSNAP IEEE-1394 Camera: Side View 16 CoolSNAP / CoolSNAP / CoolSNAP / CoolSNAP...
  • Page 28: Ccd Specifications And Orientations

    CoolSNAP / CoolSNAP CoolSNAP CoolSNAP Specifications Image Type Color and Mono Mono Mono and Orientations Array Sony ICX205AK (color) Sony ICX285AL Sony ICX285AL Sony ICX205AL (mono) Resolution 1392 x 1040 1392 x 1040 1392 x 1040 Pixel Size 4.65 µm x 4.65 µm 6.45 µm x 6.45 µm 6.45 µm x 6.45 µm Digitization Rate...
  • Page 29: Detector

    Detector Rear Panel Description The following connectors, display lights, and controls are located on the back of the cameras (pictured below). DATA connector: for data transfer and power LVDS cameras: 20-pin, high-density connector • IEEE-1394 cameras: 6-pin IEEE-1394 connector • EXPOSE OUT connector: BNC connector;...
  • Page 30: Auxiliary Power Supply

    Auxiliary Power Supply Usage Requirements The auxiliary CoolSNAP power supply (shown below) provides power to your CoolSNAP camera, but is required only under certain conditions. If your CoolSNAP camera is connected to an IEEE-1394 port that does not supply power (such as a 4-pin connector on a laptop computer) you need to use the auxiliary power supply.
  • Page 31 BENELUX FRANCE GERMANY Roper Scientific, BV Roper Scientific, SARL Roper Scientific, GmbH Ir. D.S. Tuijnmanweg 10 Z.I. Petite Montagne Sud Rosenheimer Landstr. 87 4131 PN VIANEN, Netherlands 4, rue de l'Oisans - C.E. 1702 D-85521 Ottobrunn, Germany tel: 31.347.324989 91017 Evry Cedex, France tel: 49.89.660.779.3...

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