Fisher ALPHA 1-2 LDplus Operating Manual page 30

The ampoule is then connected to the hose and the hose clamp is
removed. Thus the partial pressure in the ampoule is suddenly
decreased and a partial thawing during the evacuation process is
avoided.
One ampoule after the other is connected using this method.
Sealing of the ampoule is carried out in the same way. The hose of
the ampoule to be sealed is clamped and the ampoule is sealed under
vacuum using the blow lamp.
If the ampoule breaks during sealing the vacuum in the drying
chamber is not affected due to the clamping with the hose clamp.
The remaining glass from the ampoule is removed and the hose is
closed with a blind plug.
In this way one ampoule after the other can be sealed or new
ampoules can be connected.
Description of the Freeze Drying Processes • 23
Operating Manual Freeze Dryer ALPHA 1-2 LDplus