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Instruction Manual
HI 3856
Copper
Ultra Low Range
Test Kit
www.hannainst.com
Dear Customer,
Thank you for choosing a Hanna Product.
Please read the instruction sheet carefully before using the
chemical test kit. It will provide you with the necessary
information for correct use of the kit.
Remove the test kit from the packing material and examine
it carefully to make sure that no damage has occurred
during shipping. If there is any noticeable damage, notify
your Dealer or the nearest Hanna office immediately.
Each kit is supplied with:
• HI 3856-0 Reagent, packets (100 pcs);
• 1 long path color comparator cube.
Note: Any damaged or defective item must be returned in
its original packing materials.
SPECIFICATIONS
SPECIFICATIONS
SPECIFICATIONS
SPECIFICATIONS
SPECIFICATIONS
Range
0 to 0.25 mg/L (ppm) as Copper
Smallest Increment
0.05 ppm
Analysis Method
Colorimetric, bicinchoninate
Sample Size
25 mL
Number of Tests
100
Case Dimensions
230x59x70 mm (9.0x2.3x2.8")
Shipping Weight
180 g (6.3 oz.)
SIGNIFICANCE AND USE
SIGNIFICANCE AND USE
SIGNIFICANCE AND USE
SIGNIFICANCE AND USE
SIGNIFICANCE AND USE
Copper is an essential trace element in human diet (the
daily requirement is around 2.0 mg) and a factor in plant
metabolism. Copper salts are widely used in water supply
systems to control biological growth in reservoirs. Corrosion of
copper alloys in pipe fittings on the other hand may
introduce considerable quantities into the water supplies.
Note: mg/L is equivalent to ppm (parts per million).
CHEMICAL REACTION
CHEMICAL REACTION
CHEMICAL REACTION
CHEMICAL REACTION
CHEMICAL REACTION
Copper salts react with bicinchoninate reagent to form a
purple product in neutral buffered condition. The amount of
color developed is proportional to the concentration of copper
present in the aqueous sample.
INSTRUCTIONS
INSTRUCTIONS
INSTRUCTIONS
INSTRUCTIONS
INSTRUCTIONS
READ THE ENTIRE INSTRUCTIONS BEFORE USING THE KIT
• Fill the long path comparator cube with the sample to
the 25 mL mark.
• Add 1 packet of HI 3856-0 reagent.
• Replace the cap and mix by inverting the cube several
times.
• Wait 45 seconds to allow color to develop.
• Determine which color best matches
the solution in the cube and record
the result as mg/L (ppm) of Copper.
• It is better to match the color with a white sheet at
about 10 cm behind the comparator.
Note: To measure Copper in the 0-2.5 ppm range, use the
HI 3847 Copper Test Kit.
R E F E R E N C E S
R E F E R E N C E S
R E F E R E N C E S
R E F E R E N C E S
R E F E R E N C E S
Adapted from Nakano S., Yakugaku Zasshi,
1962
1962
1962
1962
1962, 82 486-491
[Chemical Abstract, 58 3390e (1936)].
HEALTH AND SAFETY
HEALTH AND SAFETY
HEALTH AND SAFETY
HEALTH AND SAFETY
HEALTH AND SAFETY
The chemicals contained in this kit may be hazardous if
improperly handled. Read the relevant Health and Safety
Data Sheet before performing this test.
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