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Instruction Manual
HI 3844
Hydrogen
Peroxide
Test Kit
www.hannainst.com
Dear Customer,
Thank you for choosing a Hanna Product. Please read the
instructions carefully before using the chemical test kit. It
will provide you with the necessary information for correct
use of the kit.
Remove the chemical test kit from the packing material and
examine it carefully to make sure that no damage has
occurred during shipping. If there is any noticeable dam-
age, notify your Dealer or the nearest Hanna office
immediately.
Each kit is supplied with:
SPECIFICATIONS
SPECIFICATIONS
SPECIFICATIONS
SPECIFICATIONS
SPECIFICATIONS
• HI 3844A-0 Hydrogen Peroxide Reagent, 1 bottle
(100 mL) ;
• HI 3844B-0 Hydrogen Peroxide Reagent, 1 bottle
(17g) ;
• HI 3844C-0 Hydrogen Peroxide Reagent, 1 bottle
with dropper (30 mL);
• HI 3844D-0 Hydrogen Peroxide Reagent, 1 bottle
with dropper (25 mL)
• 1 plastic test tube, graduated with cap;
• 1 calibrated plastic vessel (50 mL);
• 1 plastic pipette (3 mL);
• 1 plastic pipette (1 mL), for HI 3844A-0 Reagent;
• 1 plastic spoon.
Note: Any damaged or defective item must be returned in
its original packing materials.
SPECIFICATIONS
SPECIFICATIONS
SPECIFICATIONS
SPECIFICATIONS
SPECIFICATIONS
Range
0 to 2 ppm H
O
Low Range
2
2
0 to 10 ppm H
O
High Range
2
2
Smallest Increment
0.25 ppm H
O
Low Range
2
2
1.0 ppm H
O
High Range
2
2
Analysis Method
Drop-Count Iodometric Titration
Sample Size
25 mL Low Range
5 mL High Range
Number of Tests
100 (average)
Case Dimensions
235x175x115 mm (9.2x6.9x4.5")
Shipping Weight
450 g (15.9 oz.)
SIGNIFICANCE AND USE
SIGNIFICANCE AND USE
SIGNIFICANCE AND USE
SIGNIFICANCE AND USE
SIGNIFICANCE AND USE
Hydrogen peroxide is widely used as a disinfectant and as
a bleach for textiles, wood pulp, hair, fur etc.. It is also
used as a substitute for chlorine in water and sewage
treatment. Most common commercial forms are aqueous
solutions containing about 6, 12 and 30 per cent hydrogen
peroxide and are referred to as "20-volume", "40-volume"
and "100-volume" respectively, meaning the value of oxy-
gen liberated when the solution is boiled. The Hanna test
kit can quickly and easy determine concentration in water
up to 10 ppm of hydrogen peroxide. This is due to the fact
that it is not affected by stabilizers, which are sometimes
added to commercial hydrogen peroxide solutions. The kit is
portable and can be used in the field as well as in the
laboratory.
Note:
Note:
Note: mg/L is equivalent to ppm (parts per million).
Note:
Note:
CHEMICAL REACTION
CHEMICAL REACTION
CHEMICAL REACTION
CHEMICAL REACTION
CHEMICAL REACTION
Hydrogen peroxide is determined by a titrimetric method. It
reacts slowly with iodide in acid solution (Step 1); thus a 15
minutes interval is required to allow the reaction to occur
completely. The amount of iodine generated is equivalent to
the hydrogen peroxide in the sample. The liberated iodine
is then titrated with standard sodium thiosulfate solution
that reduces the iodine back to iodide ions (Step 2).
–
Step 1: H
O
+ 2H
+
+ 2I
I
+ 2H
O
2
2
2
2
2–
–
2–
Step 2: I
+ 2S
O
2I
+ S
O
2
2
3
4
6
INSTRUCTIONS
INSTRUCTIONS
INSTRUCTIONS
INSTRUCTIONS
INSTRUCTIONS
READ THE ENTIRE INSTRUCTIONS BEFORE USING THE KIT
• Remove the cap from the plastic vessel and rinse it with
sample. For H
O
Low Range fill it up to the 25 mL
2
2
mark with the sample. In case of H
O
High Range fill
2
2
the vessel with 5 mL of the sample using the graduated
plastic test tube and add D.I. water to the 15 mL
mark.
• Add 1 mL of HI 3844A-0 reagent for Low Range or 0.5
mL for High Range using the plastic pipette and swirl
gently to mix.
• Using the plastic spoon provided, add 1 spoon of HI
3844B-0 reagent and swirl gently to mix.
• Wait for 15 minutes to allow
reaction to occur, leaving the
plastic vessel closed and in a dark
place.
Note: if hydrogen peroxide is present, the solution will turn
to a dark yellow color.
• Slowly add drops of the titration reagent HI 3844C-0
while swirling the solution and counting the drops.
Continue adding the titration solution until the yellow
color of the solution is almost colorless.
• Add 2 to 3 drops of HI 3844D-0 reagent and mix by
carefully swirling the plastic vessel in tight circles. The
solution will turn a blue color.
• Continue adding slowly drops of the titration reagent HI
3844C-0, while swirling and counting the drops, until
the solution changes from blue to colorless.
• To obtain the concentration in ppm of Hydrogen Perox-
ide in your sample, multiply the total number of drops
of the titration reagent HI 3844C-0 used to turn the
solution from the dark yellow color to colorless by 0.25
or 1 for Low Range or High Range, respectively.
# of DROPS
0.25 = ppm Hydrogen Peroxide LR
*
# of DROPS
1 = ppm Hydrogen Peroxide HR
*
Note
Note
Note: after a few minutes, the blue color of the starch/
Note
Note
iodine complex may reappear after the titration has
been completed due to air oxidation of the iodine.
REFERENCES
REFERENCES
REFERENCES
REFERENCES
REFERENCES
Vogel's, Textbook of quantitative chemical analysis, 5
Longman Scientific and Technical
HEALTH AND SAFETY
HEALTH AND SAFETY
HEALTH AND SAFETY
HEALTH AND SAFETY
HEALTH AND SAFETY
The chemicals contained in this test kit may be hazardous if
improperly handled. Read Health and Safety Data Sheets
before performing the test.
th
ed.,
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