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Table of Contents
Related Manuals for Gilson microman M250E
Summarization of Contents
Introduction to Microman E Pipettes
Parts Checklist
Verify that the following items are present: MICROMAN E, User's Guide, Safety bag, Adhesive ID-tags, Capillary pistons, Certificate of conformity.
Good Laboratory Practice (GLP) Features
Details features like no need to touch disposables, serial number, barcode, ID Tag, color-coded push button, data matrix, and ISO 8655 conformity.
Microman E Pipette Description
Details the parts of the MICROMAN E pipette, including the color-coded push button, body, capillary, piston, shaft, thumbwheel, volumeter, volume control switch, and data matrix code.
Operating Ranges and Material Specifications
Lists available MICROMAN E models, their part numbers, volume ranges, and the materials used for different pipette components and capillary pistons.
Pipette Specifications and Validation
Explains that MICROMAN E offers excellent accuracy and precision, validated according to Gilson quality system and ISO 8655 standards, with a performance assurance level of 99.8%.
Gilson Maximum Permissible Errors
Presents tables of maximum permissible errors (systematic and random) for different MICROMAN E models and capillary pistons, according to Gilson and ISO 8655 standards.
Fitting the Capillary Piston
CP TIPACK Fitting Procedure
Details the procedure for fitting capillary pistons (CP) from the TIPACK packaging onto the MICROMAN E pipette, including pre-wetting.
CP Bulk Fitting Procedure
Explains how to fit capillary pistons (CP) from bulk packaging for CP25, CP50, and CP250 models, emphasizing care to avoid damage.
Pipetting Operations
Setting the Volume
Explains how to set the volume using the volumeter's three number dials and thumbwheel, including color coding and fine adjustment techniques.
Pre-Wetting the Capillary Piston
Discusses the importance of pre-wetting the CP for greater uniformity and precision of dispensing by providing identical contact surfaces.
Aspirating Liquid
Details the steps for aspirating liquid, including pressing the push button to the first stop, immersing the capillary, slowly releasing the button, and wiping excess liquid.
Dispensing Liquid
Describes how to dispense liquid by pressing the push button to the first stop, withdrawing the capillary, and releasing the button.
Ejecting the Capillary Piston
Explains how to eject the capillary piston by pressing the push button to the second stop, simultaneously ejecting the piston and capillary.
Troubleshooting Common Issues
Leaks
Provides troubleshooting steps for leaks, primarily suggesting to replace the capillary piston.
No Stroke
Addresses issues with the pipette stroke, suggesting to check piston fitting and test the push button operation.
Difficulty Fitting a Capillary
Offers solutions for difficulty fitting a capillary, recommending cleaning the capillary holder with ethanol.
Inaccuracy
Suggests checking the capillary mounting on the capillary holder for issues causing inaccuracy.
Imprecision
Recommends replacing the capillary piston for issues related to imprecision.
Difficulty Setting Volume
Indicates potential internal damage for difficulty setting volume and advises contacting a Gilson representative.
Persistent Problems
Advises contacting your local Gilson representative if problems persist after initial troubleshooting.
Cleaning and Decontamination Procedures
External Cleaning
Guides on cleaning the pipette externally using soap solution, distilled water, and soft cloths, with a warning about contamination before return.
Internal Cleaning
Details internal cleaning procedures, recommending immersion of specific components (clamp assembly, spring, holder) in decontaminant.
Chemical Decontamination
Explains chemical decontamination, advising to check compatibility of decontaminants with pipette materials.
Non-immersible Parts Decontamination
Provides steps for decontaminating non-immersible parts, like the body, using impregnated cloths.
Immersible Parts Decontamination
Outlines immersion procedures for internal components in decontaminant solution, following manufacturer's instructions.
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