ibidi 10902 Instruction Manual

ibidi 10902 Instruction Manual

Pump system
Table of Contents

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Instruction Manual
ibidi Pump System
Version 2.6
ibidi.com

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Table of Contents
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Summarization of Contents

Preamble and System Specifications
1.1 Introduction
Manual purpose, user guidance, and research use only statement.
1.2 Safety Symbols
Explanation of warning symbols and their meanings for safe operation.
1.4 Specifications
Technical details including electrical, environmental, and dimensional parameters.
1.6 Safety Considerations
Critical safety precautions for operating the ibidi Pump System.
Intended Use and Equipment
2 Intended Use of the ibidi Pump System
Describes the system's application in cell culture and its key advantages.
3.1 Components of the ibidi Pump System
Lists and describes all parts included in the ibidi Pump System variants.
3.2 ibidi Pump
Details the functionality and capabilities of the ibidi Pump unit.
3.3 Fluidic Unit
Explains the role and components of the Fluidic Unit, including its valves.
Setup Procedures
4 Quick Start Guide
Overview for a standard setup, guiding through initial component connections.
5 Basic Setup
Detailed connection steps for a basic setup with one fluidic unit.
6.1 Degassing of Slides, Tubing, and Medium
Essential step to prevent air bubbles by preparing components before use.
6.2 Mounting a Perfusion Set on the Fluidic Unit
Step-by-step instructions for correctly attaching the perfusion set.
6.6 Calibration of the Flow Rate
Guide to calibrate the system for accurate flow rate and shear stress measurements.
Advanced Operations and Technical Details
7.1 Working With Two or More Fluidic Units
Describes parallel setups using multiple fluidic units.
7.3 Oscillatory Flow Experiments
How to set up and perform experiments with oscillatory flow.
8.1 Working Principle of the ibidi Pump
Explains the core mechanism of flow and shear stress generation.
8.5 Shear Stress Calculations in ibidi Channel Slides
Formulas for calculating wall shear stress for various slide types.
Maintenance and Troubleshooting
9.1 Disinfection and Cleaning
Procedures for cleaning and maintaining the pump and fluidic unit.
10.1 Air Bubbles
Addresses common issues with air bubbles and their prevention or removal.
10.2 Cells are Detaching
Discusses causes and solutions for cell detachment during experiments.
10.7 Flow Rate is Too Low or Absent
Troubleshooting steps for incorrect or absent flow rates.

Table of Contents