Table of Contents

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®
NanoPhotometer
NP80/N60/N50/C40
User Manual
Version 3.1
Software Version 3.1.13220

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Table of Contents
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Summarization of Contents

NANOPHOTOMETER® APPLICATIONS
NUCLEIC ACIDS
Determines concentration, purity, and dye incorporation for nucleic acids.
PROTEIN UV
Protein UV determination at 280 nm and purity calculations.
PROTEIN ASSAYS
Colorimetric assays for protein concentration measurement.
KINETICS
Measures reaction rates and enzyme kinetics.
OD600
Measures bacterial cell density at 600 nm.
MORE APPS
Access to additional applications like wavelength, concentration, wavescan.
NANOPHOTOMETER® AT A GLANCE
NANOPHOTOMETER® OVERVIEW
General introduction to the Implen NanoPhotometer® spectrophotometer.
NANOPHOTOMETER® NP80
Details and features of the NanoPhotometer NP80 model.
NANOPHOTOMETER® N60
Details and features of the NanoPhotometer N60 model.
NANOPHOTOMETER® N50
Details and features of the NanoPhotometer N50 model.
NANOPHOTOMETER® C40
Details and features of the NanoPhotometer C40 model.
INSTRUMENT REAR PANEL (NP80/N60/N50/C40)
Identifies ports and controls on the rear panel of the instrument.
INSTRUMENT BOTTOM VIEW (NP80/N60/N50/C40)
Shows the identification plate on the bottom of the instrument.
ACCESSORIES
STANDARD ACCESSORIES
Lists essential items included with the NanoPhotometer®.
OPTIONAL ACCESSORIES
Lists available accessories that enhance instrument functionality.
CONNECTIVITY
USB A
Describes USB A ports for data transfer and device connection.
USB B
Details the USB B port for connecting to a computer.
LAN
Explains Ethernet connection for network access and data transfer.
WiFi
Setting up WiFi network and hotspot, and connecting to networks.
HDMI
Details the HDMI port for connecting to external monitors.
GETTING STARTED
SPECTROPHOTOMETER INSTALLATION
Guidance on initial setup and installation of the spectrophotometer.
SAFETY INFORMATION
Crucial safety precautions for operating the instrument.
UNPACKING AND POSITIONING
Instructions for unpacking and placing the instrument safely.
SOFTWARE INSTALLATION
Procedures for installing and managing the instrument's software.
FIRST STEPS AND CONFIGURATION WIZARD
Initial setup wizard for configuring the NanoPhotometer®.
PRINTER INSTALLATION
Instructions for installing and connecting printers.
NANOPHOTOMETER® BASICS
APPLICATIONS OVERVIEW
Introduction to the various applications available on the instrument.
ICONS
Explanation of the various icons used in the NanoPhotometer® interface.
BUTTONS
Description of the function of different buttons on the instrument interface.
SIDE TAB BAR
Explanation of the vertical tab bar for navigating measurement screens.
DATA PROCESSING DIALOGS
Print
Options and procedures for printing measurement data.
Save
Options for saving measurement data in various file formats.
BASIC OPERATION
NANOVOLUME MEASUREMENT BASICS (N50/N60/NP80)
Step-by-step guide for nanovolume measurements.
CUVETTE MEASUREMENT BASICS (C40/NP80)
Step-by-step guide for cuvette measurements.
SUBMICROLITER CELL MEASUREMENTS BASICS (C40)
Instructions for using the submicroliter cell with the C40 model.
SAMPLE HANDLING TIPS
Guidance on proper sample handling for accurate measurements.
MORE APPS
MORE APPS: WAVELENGTH
Measure absorbance at specific wavelengths.
MORE APPS: WAVESCAN
Obtain a complete spectral scan over a defined wavelength range.
MORE APPS: ABSORBANCE RATIO
Determine absorbance ratios at two specified wavelengths.
MORE APPS: CONCENTRATION
Calculate sample concentration using absorbance and a factor.
MORE APPS: STANDARD CURVE
Create and use calibration curves for sample quantification.
PREFERENCES
GENERAL
General settings including display, about, WiFi, and sample window.
DATE AND TIME
Setting the instrument's current date and time.
DISPLAY
Adjusting screen brightness and screensaver settings.
LANGUAGE
Options for changing the user interface and output language.
ILLUMINATION SAMPLE WINDOW
Toggle switch to turn the sample window illumination on or off.
ENABLE NANOVOLUME
Toggle switch to enable the submicroliter cell option for C40.
DYES
Managing pre-programmed and custom dye labels for analysis.
WARNING MESSAGES
BLANK CONTROL
Toggle switch to enable or disable Blank Control™ feature.
SAMPLE QUALITY CONTROL
Adjusting ratio alert warning message limits for quality control.
NETWORK
Configuring network settings (DHCP or static IP).
WIFI
Setting up WiFi Hotspot or connecting to a WiFi network.
NETWORK PRINTER
Instructions for connecting to network printers.
REPORT CONFIGURATION
Defining table columns for PDF files and printouts.
TROUBLESHOOTING
SELF-CALIBRATION TEST
Information on the automatic self-calibration test and error handling.
ASSISTANCE
SUPPORT
Contact information for Implen support team.
REPORT PROBLEM
How to report issues with the instrument or software.
SOFTWARE MAINTENANCE
Procedures for resetting, updating, and logging instrument data.
INSTALL PRINTER DRIVER
Steps to install necessary printer drivers.
CONTACT IMPLEN
Contact details for Implen GmbH and Implen, Inc.
MAINTENANCE
MAINTENANCE FREE TECHNOLOGY
Information that the technology is maintenance free.
REPLACEMENT PARTS
Details on replacing parts like lamps, batteries, and touchscreens.
CLEANING AND GENERAL CARE
Guidelines for cleaning and maintaining the instrument's exterior.
MESSAGES
Explains different types of messages displayed by the software.
IMPORTANT WARNING MESSAGES
Lists common warning messages and their solutions.
IMPORTANT ALERT MESSAGES
Lists critical alert messages and troubleshooting steps.

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