Laboratory cooling water monitoring unit (20 pages)
Summary of Contents for Roth MAXI-Vertical-Dual-Electrophoresis
Page 1
Instructions for use MAXI-Vertical-Dual-Electrophoresis unit Including Gel casting tray: X950.1 MAXI-Vertical-Dual-Elektrophoresis unit - Standard Tank + running module + a + casting module ccessories X951.1 MAXI -Vertical-Dual-Elektrophoresis unit - cooled cooled Tank + running module + accessories + casting module...
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They should be operated only by qualified technically trained personnel. The vertical electrophoresis units from ROTH are designed for long term laboratory use and to obtain reproducible results. Please spend a few moments reading the instruction manual thoroughly.
1.0 mm combs with 24 slots exchange electrodes Available Accessories (All accessories can be purchased from Carl Roth GmbH + Co. KG. Please use the indicated ordering numbers. Additional reagents and accessories can be found in top K.) Tank (without lid) X954.1...
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If any leakage is seen do not attempt to repair or use the apparatus, but notify Carl Roth GmbH & Co. KG immediately (+49/0721/5606-166).
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Gel 30, ROTIPHORESE Gel 40; further acrylamide stock solutions can be found in the main Roth catalogue). Acrylamide solutions should be stored in a cool, dark place (refrigerator). For casting the gels, the solutions should have room temperature. Avoid exposure to heat and sun.
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4. The gel casting can be carried out directly in the gel caster. Pour in the mixture slowly between the glass plates. Avoid the formation of air bubbles. 5. If you wish to cast a two-phase gel (collecting gel and separating gel), first cast the separating gel to app.
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® 2. Mix the protein samples with 1/3 volume ROTI Load 4 x (SDS-running buffer) or resuspend the pellet in app. 30 l 1 x ROTI ® Load. Heat the sample for 3 min to 100 °C or for 50 min to 80 °C. Centrifuge ®...
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® ® ROTI Load DNAstain 3 SYBR Green (for fragments < 500 bp) 1CN7.1 ® ROTI Mark STANDARD T851 ® ROTI Mark 10-150 T850 ® ROTI Mark ALL BLUE, prestained 2242 ® ROTI Mark TRICOLOR, prestained 8271 ® 50 bp-DNA-Ladder SYBR Green 1CN8.1 ®...
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Be sure to fill empty wells with sample buffer. Vertical smears in the bands Possibly there were dirt particles in the gel mix. Use Roth acrylamide stock solutions, making sure that the gel solution is prepared in clean glass containers. The solution can be filtered and degassed before adding APS and TEMED.
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