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HB biochrom Ultrospec 35c User Manual

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Ultrospec 35c Colorimeter
USER MANUAL
ORIGINAL INSTRUCTIONS • 5061097 REV02 •
1

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Summary of Contents for HB biochrom Ultrospec 35c

  • Page 1 Ultrospec 35c Colorimeter USER MANUAL ORIGINAL INSTRUCTIONS • 5061097 REV02 •...
  • Page 2 Intentionally blank Ultrospec 35c User Manual • 5061097 REV02 •...

  • Page 3: Table Of Contents

    Contents RELEASE NOTES ............................... 4 WELCOME ................................ 5 ESSENTIAL SAFETY NOTES ..........................5 ........................... 5 NVIRONMENTAL ONDITIONS ............................5 AZARDS AND ARNINGS ....................... 6 AFETY OTES ELATED TO ATTERIES ..............................8 AUTION OTICE ..........................8 LEANING AND ENERAL WARRANTY AND REPAIR ..........................8 ..............................

  • Page 4: Release Notes

    Release Notes Date Revision number List of changes 06 NOV 2023 Initial release 18 DEC 2023 Correction of the photometric range in the technical specifications section. Addition of “out of range” displays for values above or below the photometric range. Copyright ©...

  • Page 5: Welcome

    Life Science. Thank you for your interest in our products. We are committed to providing you with quality products and services. This manual will help you get to know your Biochrom Ultrospec 35c Colorimeter. The structure of the manual was designed to sequentially guide you through setting up your product in an optimal way.

  • Page 6: Safety Notes Related To Li-Ion Batteries

    • Include an appropriate decontamination certificate for equipment returned for repair. • Ensure that the operator of the equipment is provided with a safe working environment. • Use, store and dispose of any chemicals in accordance with manufacturer’s guidelines and local safety regulations. •...
  • Page 7 Lithium-Ion batteries continue to slowly discharge (self-discharge) when not in use or while in storage. Routinely check the battery’s charge status. This product user manual includes information on how to check battery status, as well as battery charging instructions. See sections below: Battery Maintenance and Charging. Use only Biochrom approved batteries in your Biochrom products.

  • Page 8: Caution Notice

    Many countries prohibit the disposal of waste electronic equipment in standard waste receptacles. Caution Notice The Biochrom Ultrospec 35c Colorimeter is intended for laboratory use only and can be used in research and development applications. These systems have been designed to meet the standards for electromagnetic compatibility (EMC) intended for laboratory equipment applications as well as the applicable safety requirements for electrical equipment for measurement, control, and laboratory use.

  • Page 9: Returns And Repairs

    Returns and Repairs If any defect arises within or outside the warranty period, please contact: US Office Technical Support Email support@biochrom.co.uk Online Returns form https://support.biochrom.co.uk/hc/en- us/requests/new?ticket_form_id=1500000731442 Telephone (Toll Free) +1 800 272 2775 Telephone (Outside the US) +1 508 893 8999 Address 84 October Hill Road Holliston MA 01746...

  • Page 10: Instrument Overview

    Instrument Overview General Layout 1: Sample cell compartment 2 Colour touchscreen Touchscreen Display The instrument has a 480x272-pixel resolution backlit LCD colour display with touch panel for navigating the instrument’s built-in firmware. Instrument Connections USB-C port for connecting to PC/laptop and charging USB-A port for connecting a USB flash drive the device, using the USB-C cable supplied.

  • Page 11: Instrument Data Output

    Instrument Data Output Measurement data can be exported to a USB flash drive via the USB A socket on the side of the instrument, as a comma-separated value (.csv). Data can also be exported to a PC/laptop via a web interface, using the USB cable provided.

  • Page 12: Technical Specifications

    Technical Specifications Wavelength range (nm) 450 to 640nm Beam Height 11mm (measured from bottom of cuvette) LED array (nm) 450, 500, 520, 540, 600, 640 Bandwidth (nm) 40 nm Range Absorbance: 0 to 2.5 A Accuracy <±0.05A at 1A using neutral Density Filter and flat-edge cuvette Repeatability ±0.02A at 1A in 10mm cuvette Operation Modes...

  • Page 13: Installation

    Installation Unpacking and Contents List The following items and quantities are supplied as standard with the Ultrospec 35c. Please check this contents list against the actual content in the box. If any discrepancies are found, please contact Biochrom or your local dealer.

  • Page 14: First Time Use And Initial Setup

    First Time Use and Initial Setup To power up the unit first time after unpacking, please connect the instrument to a power outlet using the USB- C cable and charger provided. Once plugged in, the unit will automatically start and show the Start Up screen after a few seconds: Start-up screen Following the Start Up screen, 1.

  • Page 15: Instrument Operations

    Instrument Operations The instrument firmware uses an intuitive menu arrangement that is navigated using the colour touchscreen. Home screen Home screen On the Home screen, the following information is displayed: Current wavelength being selected Current time and date Battery charge status. When the unit is plugged in and charging, a lightning is displayed near the battery icon.

  • Page 16: Setting Up The Wavelength

    Sample measurement button. Press to start measuring the absorbance/transmittance of the sample at the selected wavelength. This button becomes active once a reference has been done. Kinetics measurement button. Press to start kinetics measurement on the sample. This button becomes active once a reference has been done Setting up the wavelength From the Home screen, press the wavelength symbol λ...
  • Page 17 5. An advisory message will come up to notify the user no session folders have been set. Therefore, the results will not be automatically saved in the device. Close the message to continue to the measurement page. If you wish to save your measurements, a session folder needs to be created selected...
  • Page 18 Multiple samples can be compared with the same reference by placing different samples in the cuvette holder and making measurements for each one by pressing the “sample” button. It is recommended to re-reference with the reference solution every 10 to 15 minutes to avoid any slow instrument drift.

  • Page 19: Making A Kinetics Measurement

    Making a Kinetics Measurement The kinetics mode provides a continuous readout of changes in absorbance of a sample. 1. Select the required wavelength as described in the paragraph Setting up the wavelength 2. Select Abs or %T mode by pressing the unit symbol 3.
  • Page 20 8. Set the Duration by scrolling left or right 9. Set the time interval between measurements 10. Press the Set button to save the settings. 11. Press Start to begin the measurements. The instrument will perform the number of measurements at the set intervals 12.

  • Page 21: Settings

    Settings To access the instrument’s settings page, press the cog icon on the top right corner of the display: Session Folder 1. This section allows the user to create new folders or select existing folders that will be used to store sample results.
  • Page 22 Alarm 1. This section allows the user to enable/disable various alarms on the unit. Toggle the alarm ON or OFF according to your preference for each of the features displayed. 2. Use the slider to adjust the volume of the audible alarm between 0% (no audible signal) to 100%.
  • Page 23 Date & Time 1. This section allows the user to set the time, time format, date and date format as required. 2. 24h Clock: this button allows to switch between 12h (am/pm) and 24h clock format. 3. Time: this is where the user can set the hour and minutes.
  • Page 24 9. Press the “USB-A Test” button to test the connectivity with a USB flash drive. The instrument will report whether the connectivity is established with an external flash drive. 10. NOTE: a USB flash drive must be inserted in the USB-A port to conduct this test.

  • Page 25: Data Management

    Data Management Creating a New Session Folder 1. Click on the tag icon in the top right corner to create a folder to save data. If a folder has already been selected, the tag will be displayed in blue with the folder name next to it. By clicking on it, it will open the session folder menu, listing all available folders that have been created (if any).
  • Page 26 Deleting Sample Data and/or Session Folders 1. Click on the folder icon. 2. Highlight the folder of interest and click on “View Folder” 3. Select the sample data that needs deleting and press the “Delete” button. 4. Confirm the deletion by pressing “Confirm”. 5.
  • Page 27 6. Confirm the deletion by pressing “Confirm”. NOTE: If no session folders have been selected before making any measurements, then results will not be saved on the device. Transferring Data onto a USB Flash Drive 7. Insert a USB flash drive onto the USB-A port located on the side of the unit. USB flash drives with FAT32 format must be used for proper compatibility.
  • Page 28 3. Highlight the folder of interest and click on “Transfer” 4. Select “PC Cable” then open a Web browser page and enter the URL specified on the device in the address bar. 5. The device web server page will open. 6.

  • Page 29: Useful Calculations

    Useful Calculations Beer-Lambert Law A = cεl A is the absorbance, which although unit-less is usually described as A or AU (absorbance units). C is the concentration in molar units (M). ε is the molar extinction coefficient in per molar unit per cm (M l is the pathlength in centimetres (cm).

  • Page 30: Troubleshooting

    Troubleshooting Message Displayed Details/Possible Causes Remedies Power-on self-test has detected This light sensor has Please contact Technical Support an error with the device: malfunctioned and is not working for further advice properly. Possible issues: LED sensor failure. • The sensor is broken and Please contact a service no longer works.
  • Page 31 transferring data to make curves reaches 90% capacity (45 deleting data to make additional additional space. of 50 total). space. The device has reached storage The maximum number of session Transfer or delete data to make capacity. Please delete or folders has been reached (50 space.

  • Page 32: Accessories, Spares & Services

    Accessories, Spares & Services Accessories & Consumables Part Number Description 80-3000-57 Adapter set for 10 and 12mm test tubes 80-2004-53 Pack of 100 disposable cuvettes, 1mL min. volume 80-2084-11 Pack of 100 disposable cuvettes, 0.5mL min. volume Spare parts Part Number Description 80-3007-99 User replaceable battery...

  • Page 33: Contact Information

    Contact Information Biochrom Ltd. Biochrom U.S. Unit 7, Enterprise Zone, 3970 Cambridge Research Park 84 October Hill Road Beach Drive, Waterbeach, Cambridge, UK, CB25 9PE Holliston, Massachusetts 01746 Phone: +44 1223 423 723 Phone: +1 508 893 8999| toll-free +1 800 272 2775 Harvard Bioscience (China) Co., Ltd.

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