Page 1 Translation of the original operating manual sb-bs Freeze-dryer Rev. 2.17 of 20/11/2024 Alpha 1-4 LSCplus;Freeze-dryer;Alpha 2-4 LSCplus Version 01/2011 Alpha 1-4 LSCplus Part no. 102141, 102143 Alpha 2-4 LSCplus Part no. 102142, 102144, 102150, 102152 Freeze-dryer Operating Manual Please retain for later use!
Page 2 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus Version 01/2011, Rev. 2.17 of 20/11/2024 • sb-bs 2 / 134 Translation of the original operating manual...
Page 3 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus In case of inquiries, please state the following numbers: Order number: Serial number: © Copyright by Martin Christ Gefriertrocknungsanlagen GmbH An der Unteren Söse 50 37520 Osterode am Harz Germany Tel.: +49 (0) 5522 / 5007-0...
Page 4 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus Version 01/2011, Rev. 2.17 of 20/11/2024 • sb-bs 4 / 134 Translation of the original operating manual...
Page 5: Table Of Contents
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus Table of contents General information ......................9 Importance of the operating instructions ................9 Intended use ........................9 1.2.1 Freeze-drying of solvent-containing products (non-aqueous media) ......10 1.2.2 Freeze-drying of acid-containing products ..............11 Warranty and liability .....................
Page 6 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus Table of contents 3.7.7 Oil-sealed vacuum pump: hazards caused by contaminated vacuum pump exhaust gases ......................... 30 3.7.8 Oil-sealed vacuum pump: hazards caused by hot and contaminated vacuum pump oil ........................... 31 3.7.9...
Page 7 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus Table of contents 6.5.1.2 Main window "Program" ..................62 6.5.1.3 Main window "Options " ..................63 6.5.1.4 Main window "?" ....................74 6.5.2 Manual freeze-drying ....................74 6.5.2.1 Entering set values in the manual mode............. 75 6.5.3...
Page 8 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus Table of contents 10.1 Ambient conditions ...................... 109 10.2 Technical documentation ..................... 109 Appendix .......................... 110 11.1 Mathematical relations ....................110 11.2 Brief operating instructions ..................111 11.3 EC declaration of conformity in accordance with the EC Machinery Directive ....117 11.4...
Page 9: General Information
No unauthorised design modifications, additions or conversions without the written approval by Martin Christ Gefriertrocknungsanlagen GmbH have been performed. • The vacuum pump used is approved by Martin Christ Gefriertrocknungsanlagen GmbH. The vacuum pumps approved for this system are listed in chapter 5.6 - "Vacuum pumps". •...
Page 10: Freeze-Drying Of Solvent-Containing Products (Non-Aqueous Media)
Exclusion of liability Any use of the freeze-dryer other than the one described here or any use going beyond this description is regarded as improper use. Martin Christ Gefriertrocknungsanlagen GmbH cannot be held liable for any damage resulting from such improper use.
Page 11: Freeze-Drying Of Acid-Containing Products
Otherwise, there is a risk of damage to property and personal injury. Consultation of Martin Christ Gefriertrocknungsanlagen GmbH is absolutely mandatory in order to define the measures that need to be taken! →...
Page 12: Warranty And Liability
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 1 General information Warranty and liability Our general terms and conditions apply. They have been handed over to the operator upon the conclusion of the contract. Warranty and liability claims are excluded if they are due to one or several of the following reasons: •...
Page 13: Copyright
Freeze-dryer Alpha 2-4 LSCplus 1 General information → Copyright The copyright concerning the operating manual remains with Martin Christ Gefriertrocknungsanlagen GmbH. The operating manual is solely intended for the operator and their personnel. It includes instructions and information that may not be •...
Page 14: Layout And Mode Of Operation
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 2 Layout and mode of operation → Layout and mode of operation → Layout of the freeze-dryer → 2.1.1 Functional and operating elements Ice condenser chamber with an internal ice condenser User interface (see chapter 6.5.1 - "User...
Page 15 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 2 Layout and mode of operation Aeration valve Media drain valve Fig. 3: Left side of the freeze-dryer 10 Touchpanel Fig. 4: User interface with touchpanel Version 01/2011, Rev. 2.17 of 20/11/2024 • sb-bs...
Page 16: Name Plate
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 2 Layout and mode of operation 11 Vacuum connection 12 Name plate (see chapter 2.1.2 - "Name plate") 13 Heat exchanger of the refrigeration unit 14 Data interface 15 Electrical connection of the vacuum sensor...
Page 17: Mode Of Operation
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 2 Layout and mode of operation → Mode of operation → 2.2.1 General information on freeze-drying What is freeze-drying? Freeze-drying or lyophilisation is a procedure for the gentle drying of high- quality products. The product is dried by → sublimation without passing through the liquid phase.
Page 18 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 2 Layout and mode of operation Fig. 7: Vapour pressure curve for ice and water From a physical point of view, the freeze-drying process covers three phases (see figure below): (1) Freezing: The product to be dried is frozen under atmospheric pressure.
Page 19: Freeze-Drying Process
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 2 Layout and mode of operation Fig. 8: Freeze-drying phases Please find further information about basic principles, optimum procedures and applications in the brochure "Smart freeze-drying", which can be downloaded at www.martinchrist.de → [Applications] →...
Page 20: Freezing
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 2 Layout and mode of operation → 2.2.2.2 Freezing First, the product that is to be dried is frozen. Especially in the case of small filling quantities, we recommend pre-cooling the shelves as well in order to prevent the product from thawing during the evacuation.
Page 21: Final Drying
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 2 Layout and mode of operation → 2.2.2.4 Final drying Final drying is an option whenever one requires a product with minimal residual moisture. In the physical sense, this process is a desorption process, i.e.
Page 22: Safety
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 3 Safety → Safety → Marking of the unit The following symbols are used for Christ freeze-dryers: Dangerous voltage On (Power) Hot surface Off (Power) Name plate (see chapter Caution! Risk of bruising 2.1.2 - "Name plate")
Page 23: Explanation Of The Symbols And Notes
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 3 Safety → Explanation of the symbols and notes This operating manual uses the following names and symbols to indicate hazards: This symbol stands for a direct hazard to the life and health of persons.
Page 24: Responsibility Of The Operator
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 3 Safety → Responsibility of the operator The operator is the person who uses the freeze-dryer for commercial or economic purposes or lets a third party use it and who bears the legal product responsibility for the protection of the users, personnel or third parties during operation.
Page 25: Chapter 5.6 - "Vacuum Pumps")
3 Safety • ensure that the freeze dryer is operated with one of the vacuum pumps approved by Martin Christ Gefriertrocknungsanlagen GmbH (see chapter 5.6 - "Vacuum pumps"). • have the system maintained at regular intervals (see chapter 8 - "Maintenance and service").
Page 26: Informal Safety Notes
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 3 Safety Operating personnel (users) The operating personnel operates and monitors the freeze-dryer during normal operation and within the framework of its intended use. In the event of malfunctions or other problems, the operating personnel informs the respective specialised personnel.
Page 27: Safety Notes Concerning The Transport, Set-Up And Connection And Initial Start-Up Of The Freeze-Dryer
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 3 Safety Safety notes concerning the transport, set-up and connection and initial start-up of the freeze-dryer The following notes and instructions must be observed in order to protect all persons and property.
Page 28: Hazards Caused By Improper Connection
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 3 Safety 3.6.4 Hazards caused by improper connection Risk of injury caused by consequences of improper connection Improper connection may lead to a hazardous electrical incident at a later time during the operation of the freeze-dryer.
Page 29: Hazards Caused By The Refrigeration System (Natural, Flammable Refrigerants)
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 3 Safety 3.7.2 Hazards caused by the refrigeration system (natural, flammable refrigerants) Risk of explosion due to refrigerants The refrigerants used are highly flammable and can form an explosive mixture if their concentration in the ambient air is sufficiently high.
Page 30: Hazards Caused By Acids In The Products
This may lead to severe injuries. DANGER Freeze-drying of products containing acids is only permissible if special protective measures and equipment-related precautions are taken! Consultation of Martin Christ Gefriertrocknungsanlagen GmbH is absolutely mandatory in order to define the measures that need to be taken! •...
Page 31: Oil-Sealed Vacuum Pump: Hazards Caused By Hot And Contaminated Vacuum Pump Oil
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 3 Safety 3.7.8 Oil-sealed vacuum pump: hazards caused by hot and contaminated vacuum pump oil Risk of scalding caused by the vacuum pump oil When working on the vacuum pump and exhaust gas filter (especially when changing the oil or filter), the maintenance personnel are exposed to the hot vacuum pump oil.
Page 32: 3.7.10 Hazards Caused By Noise
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 3 Safety 3.7.10 Hazards caused by noise Risk of noise-induced hearing loss Depending on the local conditions, a harmful sound pressure level may occur. High sound pressure levels can cause noise-induced hearing loss.
Page 33: Safety Devices
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 3 Safety → Safety devices → 3.8.1 System check An internal system check system monitors the data transfer and sensor signals with regard to plausibility. Errors are detected by continuous self- monitoring of the system. Error messages are displayed in the main window under "Process &...
Page 34: Maintenance And Cleaning Of The Freeze-Dryer
• Stop the freeze-dryer immediately in the event of a malfunction. Eliminate the malfunction (see chapter 7 - "Malfunctions and error correction") or contact the after-sales service of Firma Martin Christ Gefriertrocknungsanlagen GmbH (see chapter 7.3 - "Service contact"). •...
Page 35: Remaining Hazards
→ 3.12 Remaining hazards All Christ freeze-dryers were built state-of-the-art and according to the accepted safety rules. Danger to life and limb of the operator, or of third parties, or impairments of the units or other material assets, however, cannot be completely excluded when the units are being used.
Page 36: Storage And Transport
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 4 Storage and transport → Storage and transport → Dimensions and weight Values for the freeze-dryer without a vacuum pump: Alpha 1-4 LSCplus Alpha 2-4 LSCplus Height: 415 mm 415 mm Width:...
Page 37: Notes On Transport
Fig. 9: Lifting the freeze-dryer The freeze-dryer Alpha 1-4 LSCplus weighs approx. 48 kg! The freeze-dryer Alpha 2-4 LSCplus weighs approx. 60 kg! CAUTION • When setting the unit down, ensure that the feet are upright (see figures below).
Page 38: Packaging
Lift the freeze-dryer upwards and out of the crate/cardboard box. When lifting the unit, always reach under the freeze-dryer from the side. The freeze-dryer Alpha 1-4 LSCplus weighs approx. 48 kg! The freeze-dryer Alpha 2-4 LSCplus weighs approx. 60 kg! CAUTION •...
Page 39: Set-Up And Connection
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 5 Set-up and connection → Set-up and connection → Location of use Use the freeze-dryer solely in closed and dry spaces. Refrigeration problems of the freeze-dryer are often caused by insufficient conditions at the location of use. This is why compliance with the following...
Page 40: Power Supply
The operating voltage on the name plate must correspond to the local supply voltage! DANGER Christ freeze-dryers are units of protection class I. Freeze-dryers of this type have a three-wire power cord with an IEC C13 connector (see chapter 10 - "Technical data").
Page 41: Media Drain Valve
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 5 Set-up and connection → Media drain valve Risk of poisoning/infection caused by contaminated condensate (defrosting water) The condensate may contain harmful substances originating from the product. WARNING Contact with the condensate may cause severe damage to health.
Page 42 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 5 Set-up and connection • Switch the unit off by actuating the mains power switch. • Take the vacuum sensor out of its original packaging and fasten it to the connector with a bow-shaped connecting piece, two clamping rings (DIN16KF) and two centring rings (included in the scope of supply).
Page 43: Vacuum Pumps
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 5 Set-up and connection 5.4.6 Vak uumpumpe → Vacuum pumps To ensure their intended use, the freeze-dryers Alpha 1-4 and Alpha 2-4 must be used with one of the following vacuum pumps: The dimensioning of the vacuum pump depends on the accessories installed on the system.
Page 44 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 5 Set-up and connection The vacuum pump is supplied with power by the unit, but the maximum current for the vacuum pump is limited. If the current requirement of the vacuum pump is higher than the value that is stated on the label, the pump must be supplied separately via an on-site power socket.
Page 45: Dry-Running Vacuum Pump And Silencer
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 5 Set-up and connection 5.6.2 Dry-running vacuum pump and silencer Danger of poisoning/infection due to exhaust air The exhaust air may contain harmful substances originating from the product. Contact with particles in the exhaust air (especially by inhalation) may WARNING result in severe damage to health.
Page 46 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 5 Set-up and connection The vacuum exhaust gases of the dry-running vacuum pump are completely free from oil particles. However, small quantities of debris from worn seals may be emitted. That is why, even when working with non-...
Page 47: Use Of An Interface Box
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 5 Set-up and connection Use of an interface box The current consumption of certain vacuum pumps is higher than what is permissible for use with the freeze-dryer (see the label stating the maximum current values for the vacuum pump at the IEC C13 connector, illustration above).
Page 48 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 5 Set-up and connection IEC C13 connector for the vacuum pump Abb. 15: Connector for the vacuum pump at the interface box • Connect the built-in connecting cable of the interface box (see the following picture, item 2) to the IEC C13 connector for the vacuum pump at the back of the freeze dryer (item 4).
Page 49: Pressure Control Valve
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 5 Set-up and connection IEC C13 connector for the vacuum pump at the back of the freeze-dryer Fig. 17: Back of the freeze-dryer (example) → Pressure control valve The pressure control valve is integrated in the suction pipe between the vacuum pump and ice condenser chamber.
Page 50: Rubber Valves
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 5 Set-up and connection → Rubber valves The rubber valves (part no. 121860) enable the connection of round-bottom flasks, wide-neck filter bottles, or distributors for ampoules to a manifold or drying chamber. Depending on the connector of the components, the blue plug can be removed.
Page 51: Operation
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 6 Operation Operation Initial start-up Before the initial start-up, please ensure that your freeze-dryer is properly set up and installed (see chapter 5 - "Set-up and connection") WARNING → Installation of accessories The accessories must be completed in accordance with the drying method that is applied as well as in accordance with the scope of supply.
Page 52: Lscplus Control System
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 6 Operation LSCplus control system The control system LSCplus (Lyo Screen Control plus) was specifically developed for the control of freeze-drying processes. The clear user interface enables the intuitive operation of the unit.
Page 53: Main Window "Manual
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 6 Operation Program This area is used to create and execute programs for automating the freeze-drying process and for making it reproducible. This function is only available in combination with the programmer module PGMplus option.
Page 54 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 6 Operation No USB storage device or LAN network connected symbol USB storage device connected Process recording on a USB storage device active Network available, but no network drive connected Network drive connected (e.g. LPCplus, LyoLogplus)
Page 55 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 6 Operation • Select the desired configuration or quit the dialog box by pressing the "return" button. In this way, it is possible to configure a personalised overview of the actual values.
Page 56: Chapter 6.5.3.1 - "Creating A Program")
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 6 Operation Fig. 26: Dialogue box "USB process recording" Option: Pressure increase test (only with the → double-chamber method) The → pressure increase test can only be performed when the freeze-dryer is equipped with an intermediate valve. The performance is possible in the manual mode as well as in the program mode.
Page 57 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 6 Operation Dialog box "Schematic system diagram" (6) Pressing the button "Schematic system diagram" displays a schematic diagram of the system on the left-hand side of the screen, including all the components. Active components are displayed in green. Touching a component calls up its name and →...
Page 58 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 6 Operation The advantage of this system is that malfunctions that occurred during the night can be discovered the next day even if the cause of the malfunction has already been eliminated.
Page 59 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 6 Operation Error message Error code Detailed information Error counter and time stamp of the last error Arrow keys Fig. 31: Details concerning an error message The texts of the process and error messages are not included in this operating manual.
Page 60 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 6 Operation Standard unit test Apart from the process phases of the freeze-drying process ("Freezing", "Warm-up", "Main drying", and "Final drying") and the operating mode "Defrosting", the button "Unit Test" is also available. This button opens a selection of a test with fixed parameters.
Page 61 Freeze-dryer Alpha 2-4 LSCplus 6 Operation Fig. 34: Results of the performance tests Evaluation: Please contact the Martin Christ Gefriertrocknungsanlagen GmbH for an assessment of the results. Button "Stop" (9) Pressing this button stops the current process. The system switches to the standby status.
Page 62: Main Window "Program
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 6 Operation 6.5.1.2 Main window "Program" In the main window "Program", pre-programmed freeze-drying processes can be loaded and edited and new programs can be created with the PGMplus programmer module. For this function, the PGMplus programmer module must be available.
Page 63: Main Window "Options
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 6 Operation 6.5.1.3 Main window "Options " The main window "Options" is used to perform fundamental basic settings of the control system in order to adapt it perfectly to the respective area of application of the freeze-dryer.
Page 64 • Double chamber method LyoCube (outside): drying outside the ice condenser chamber, but with the Christ LyoCube (a rectangular drying chamber that can be loaded from the front) Fig. 39: Dialog box "Freeze-drying processes" Version 01/2011, Rev. 2.17 of 20/11/2024 • sb-bs...
Page 65 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 6 Operation Administration "Administration" options: "Date/Time" "Network" "System properties" "Access protection" "Device options" "Configuration of sensors"" Fig. 40: Dialogue box "Options" / "Administration" Date/Time (1) The LSCplus control system is equipped with an integrated, battery- buffered clock.
Page 66 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 6 Operation Network (2) This dialog box is used to change various system settings. The modifications will not become effective until after a restart of the unit. NOTE Fig. 42: Dialog box "Network"...
Page 67 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 6 Operation Click on touch: If this function is active, a clicking sound can be heard whenever the system registers that a button has been touched. Behaviour of the pressure control valve in the case of insufficient ice condenser cooling: If this function is active (button "closed"), the pressure...
Page 68 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 6 Operation • The button with the lock symbol blocks any access immediately and the system switches to the default setting. Fig. 46: Access blocked, the data cannot be edited User/maintenance/administrator password: For each of these access levels, certain editing rights have been defined.
Page 69 Freeze-dryer Alpha 2-4 LSCplus 6 Operation • Enter the six-digit Christ activation code that was supplied for this option. Note that the keys are case sensitive. Options can be removed in the same way. The modifications will not become effective until after a restart of the unit.
Page 70 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 6 Operation Service "Service" options: "Operating hours" "Failure memory" "Wireless shelf technology" "Vacuum pump oil change" "Maintenance" "Sensor adjustment" Fig. 49: Dialog box "Service" (varies depending on the type of system) Operating hours (1) This dialog box is used to call up the number of operating hours of the various components of the freeze-dryer, e.g.
Page 71 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 6 Operation Always indicate the error code in the event of enquiries or service requests! NOTE Fig. 51: Dialog box "Failure memory" Wireless Shelf Technology (3) This function depends on the unit type and is not available for all types of freeze-dryers.
Page 72 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 6 Operation • In order to assign an address, open the dialog box "Wireless Shelf Technology". • Ensure that only the WST module to which an address is to be assigned is connected. If several modules are connected, they will all receive the same address, resulting in a communication conflict.
Page 73 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 6 Operation Fig. 54: Dialog box "Maintenance" Sensor adjustment (6) In this dialogue box, the sensors are adjusted based on a predefined reference value. Sensors that are not properly adjusted will lead to incorrect measurements which, in turn, will have a negative effect on the process control.
Page 74: Main Window
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 6 Operation 6.5.1.4 Main window "?" This main window includes the most important information concerning your freeze-dryer: Freeze-dryer type Control system type Serial number Manufacturer contact data Details concerning the software version Fig.
Page 75: Entering Set Values In The Manual Mode
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 6 Operation If in one section the value "∞" (infinite) is selected for a process phase, the next phase must be started manually via the button "Operating mode: select/start". NOTE In the manual mode, the set values of the active phase can be changed during the process run.
Page 76 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 6 Operation "Aeration" button "Defrosting" button "Media drain" button (special equipment) Fig. 57: Dialog box "Set values: view/edit" Fields that are displayed in the form of buttons can be edited. • Aeration (1) If the freeze-dryer is equipped with an automatic aeration valve, this button is used to define the aeration pressure.
Page 77: Option: Freeze-Drying With The Pgmplus Programmer Module
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 6 Operation • Confirm the new value and quit the numerical keypad by pressing the button "Accept". • Confirm the input and quit the dialog box via the button "Accept". • If the dialog box is closed by the button "return", the changes will be discarded.
Page 78 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 6 Operation T/°C Pressure/mbar 1000 Set value of the previous section Course of the shelf temperature control set value 0.01 Set value of the current section 0.001 0.0001 Previous Following Current section...
Page 79 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 6 Operation this limit, the shelf heater will be switched off in order to prevent the product from thawing due to excessive energy input by the shelf temperature control system. The LyoControl value is checked only during the main and final drying phases.
Page 80: Creating A Program
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 6 Operation 6.5.3.1 Creating a program To create a new program: • Press the button "New program" in the main window "Program". The dialog box that is displayed offers various different program templates.
Page 81 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 6 Operation “Program” buttons: "Program" "Program name" "Show diagram " "Insert section " "Delete section " Button for calling up more functions and set values "Save" Fig. 63: Editing a blank program template •...
Page 82 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 6 Operation Button ">>more" The button ">>more" can be used to enter more functions and set values in a program-related manner. Fig. 64: Selection of program-related functions and set values Vacuum pump warm-up See (see chapter 6.6 - "Optional extensions")
Page 83 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 6 Operation initiated. Otherwise, a process message will be issued, the current section will be extended, and further pressure increase tests will be executed periodically until the condition is fulfilled. If the actual value exceeds the set value before the preselected time has elapsed, the test will be aborted in order to prevent the product from thawing.
Page 84 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 6 Operation Option: Comparative pressure measurement (see chapter 6.6 - "Optional extensions") The indication of the measurement value "dp Comp" can be configured in chapter 6.5.1.1 - "Main window "Manual""under "Actual values of the current process"...
Page 85: Editing A Program
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 6 Operation Program templates (2) The PGMplus programmer module offers 14 different program templates that include recipes for all kinds of freeze-drying applications. They are suggestions for orientation purposes and must be adapted to the specific area of application of the freeze-dryer.
Page 86: Copying A Program
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 6 Operation 6.5.3.3 Copying a program If a new program is to be created based on an already existing program, the already existing program can be copied. A free program storage location must be available for this purpose.
Page 87: Deleting A Program
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 6 Operation Fig. 69: The freeze-drying process can be started with the aid of the button “Program start”. • During the freeze-drying process, the description of the active program can also be called up from the main window "Program" and via the button "Info".
Page 88: Optional Extensions
During the freezing process, the resistance increases. The LyoRx control sensor measures the electrical resistance. LyoLogplus data logging software LyoLogplus is a data logging software program by Martin Christ Gefriertrocknungsanlagen GmbH that is specifically adapted to the requirements of freeze-drying processes. Apart from the graphical representation of the measurement data of currently running processes, it also enables the data export for additional evaluation.
Page 89: Switching The Freeze-Dryer Off
Freeze-dryer Alpha 2-4 LSCplus 6 Operation LPCplus SCADA system The Christ LPCplus system consists of the Supervisory Control And Data Acquision (SCADA) software program by Martin Christ Gefriertrocknungs- anlagen GmbH and a dedicated PC. The system is connected to the...
Page 90: Malfunctions And Error Correction
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 7 Malfunctions and error correction → Malfunctions and error correction Malfunctions are displayed in the dialogue box "Process & equipment messages" (see chapter 7.2 - "Process and error messages"). An acoustic signal sounds when an error message is generated.
Page 91: Power Failure
(see chapter 7.3 - "Service contact") If it is impossible to eliminate the errors, contact the Christ service department! NOTE → 7.1.1 Power failure The control system continues with the process after a power failure.
Page 92: Insufficient Vacuum
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 7 Malfunctions and error correction → 7.1.2 Insufficient vacuum The vacuum checks must be carried out when the ice condenser is frozen. NOTE → 7.1.2.1 Small flange connections Leakages are often due to improper small flange connections between the various components and hose connections or to leakages in the valves.
Page 93: Aeration Valve, Media Drain Valve
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 7 Malfunctions and error correction → 7.1.2.2 Aeration valve, media drain valve Risk of poisoning/infection caused by contaminated condensate (defrosting water) The condensate may contain harmful substances originating from the product. WARNING Contact with the condensate may cause severe damage to health.
Page 94: Insufficient Ice Condenser Temperature
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 7 Malfunctions and error correction → 7.1.3 Insufficient ice condenser temperature Ensure sufficient ventilation. Do not place any paper, cloth, or similar material behind or under the unit, since otherwise the air circulation will be impaired.
Page 95: Service Contact
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 7 Malfunctions and error correction → Service contact In the event of queries, malfunctions, or spare part enquiries: From Germany: Contact Martin Christ Gefriertrocknungsanlagen GmbH An der Unteren Söse 50 37520 Osterode (Germany) Tel.
Page 96: Maintenance And Service
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 8 Maintenance and service → Maintenance and service The freeze-dryer and the accessories are subject to high mechanical and chemical stress. Thorough maintenance performed by the user extends the service life and prevents premature failure.
Page 97: Chapter 8.2 - "Disinfection Of The Drying Chamber And Accessories")
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 8 Maintenance and service Cleaning of the freeze-dryer Risk of burns on hot surfaces After a drying process, some or all of the surfaces inside the chamber may still be hot. There is a risk of burns when touching the surfaces.
Page 98: Aeration Valve, Media Drain Valve
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 8 Maintenance and service → 8.1.3 Aeration valve, media drain valve Risk of poisoning/infection caused by contaminated condensate (defrosting water) The condensate may contain harmful substances originating from the product. WARNING Contact with the condensate may cause severe damage to health.
Page 99: Heat Exchanger (Only For Air-Cooled Freeze-Dryers)
• Check the heat exchanger at least once per month for soiling and clean it if necessary. • Please contact the Christ service department if you have any queries (see chapter 7.3 - "Service contact"). 8.1.5 Electrical system Danger of life caused by electric shock There is a risk of electric shock when touching current-carrying components.
Page 100: Vacuum Pump
The other oil changes depend on the operating conditions. In general, an interval of 500 to 1,000 operating hours is sufficient. • Please contact the Christ service department if you have any queries (see chapter 7.3 - "Service contact"). →...
Page 101: Refrigeration System
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 8 Maintenance and service 8.1.8 Refrigeration system Risk of explosion due to refrigerants The natural refrigerants used are highly flammable and can form an explosive mixture if their concentration in the ambient air is sufficiently high.
Page 102: 8.1.10 Accessories
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 8 Maintenance and service → 8.1.10 Accessories For the care of the accessories, special safety measures must be considered as these are measures that will ensure operational safety at the same time.
Page 103: Service
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 8 Maintenance and service Service In the event of service work that requires the removal of the panels, there is a risk of electric shock or mechanical injury. Only qualified specialist personnel is authorised to perform this service work.
Page 104 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 8 Maintenance and service 1. Declaration of decontamination As a certified company and due to the legal regulations for the protection of our employees and of the environment, we are obliged to certify the harmlessness of all incoming goods.
Page 105: Disposal
This symbol means that it is not permissible to dispose of the freeze-dryer among household waste. • You can return these freeze-dryers free of cost to Martin Christ Gefriertrocknungsanlagen GmbH. • Ensure that the freeze-dryer is decontaminated. Fill in a declaration of decontamination.
Page 106: 10 Technical Data
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 10 Technical data → 10 Technical data Manufacturer: Martin Christ Gefriertrocknungsanlagen GmbH An der Unteren Söse 50 37520 Osterode (Germany) Type: Alpha 1-4 LSCplus Alpha 2-4 LSCplus Part number: 102141 (230 V / 50 Hz, air-cooled)
Page 107 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 10 Technical data Connection requirements (without Alpha 1-4 LSCplus Alpha 2-4 LSCplus vacuum pump and accessories) Apparent power: 0.8 kVA (at 230 V / 50 Hz) 1.7 kVA (at 230 V / 50 Hz) 1.1 kVA (at 230 V / 60 HZ)
Page 108 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 10 Technical data Equipment connections Alpha 1-4 LSCplus Alpha 2-4 LSCplus Aeration: Hose nozzle DN6 Hose nozzle DN6 (outside diameter 10 mm max.) (outside diameter 10 mm max.) Drain: Hose nozzle DN10...
Page 109: Ambient Conditions
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 10 Technical data 10.1 Ambient conditions → 10.1 Umgebungs beding ung en • Use in closed spaces • Altitudes up to 2,000 m • Ambient temperature between +5°C and +25°C • Maximum relative humidity of 80% •...
Page 110: 11 Appendix
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 11 Appendix → 11 Appendix → 11.1 Mathematical relations The automatic processes in the “Programmer module” menu (see chapter 6.5.3 - "Option: freeze-drying with the PGMplus programmer module") are based on the following considerations: T/°C...
Page 111: Brief Operating Instructions
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 11 Appendix → 11.2 Brief operating instructions Functional and operating elements Ice condenser chamber with an internal ice condenser User interface Mains power switch Fig. 76: Total view of the freeze-dryer Contact piece...
Page 112 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 11 Appendix Aeration valve Media drain valve Fig. 78: Left side of the freeze-dryer 10 Touchpanel Fig. 79: User interface with touchpanel Version 01/2011, Rev. 2.17 of 20/11/2024 • sb-bs 112 / 134...
Page 113 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 11 Appendix 11 Vacuum connection 12 Name plate 13 Heat exchanger of the refrigeration unit 14 Data interface 15 Electrical connection of the vacuum sensor 16 Connection of the vacuum sensor 17 Option: USB port...
Page 114 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 11 Appendix Step-by-step instructions – shelf drying 1 Freeze the sample separately, e.g. in a deep-freeze. Ensure that the layer thickness of 1 to 2 cm is not exceeded, since otherwise the drying time needs to be extended.
Page 115 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 11 Appendix 16 Switch the unit on again and start the defrosting process (button "Operating mode: select/start" – "Defrosting”). Ensure that no water gets into the pipe connection of the vacuum pump or vacuum sensor.
Page 116 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 11 Appendix 8 The end of the process is reached when the ice condenser is no longer loaded and when it again reaches a final temperature of approximately - 50°C to -54°C. The pressure decreases as a function of the ice condenser temperature.
Page 117: Ec Declaration Of Conformity In Accordance With The Ec Machinery Directive
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 11 Appendix → 11.3 EC declaration of conformity in accordance with the EC Machinery Directive Version 01/2011, Rev. 2.17 of 20/11/2024 • sb-bs 117 / 134 Translation of the original operating manual...
Page 118 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 11 Appendix Version 01/2011, Rev. 2.17 of 20/11/2024 • sb-bs 118 / 134 Translation of the original operating manual...
Page 119: Ukca Declaration Of Conformity
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 11 Appendix 11.4 UKCA declaration of conformity Version 01/2011, Rev. 2.17 of 20/11/2024 • sb-bs 119 / 134 Translation of the original operating manual...
Page 120 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 11 Appendix Version 01/2011, Rev. 2.17 of 20/11/2024 • sb-bs 120 / 134 Translation of the original operating manual...
Page 121: Declaration Of Conformity - China Rohs 2
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 11 Appendix 11.5 Declaration of conformity – China RoHS 2 Version 01/2011, Rev. 2.17 of 20/11/2024 • sb-bs 121 / 134 Translation of the original operating manual...
Page 122 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 11 Appendix Version 01/2011, Rev. 2.17 of 20/11/2024 • sb-bs 122 / 134 Translation of the original operating manual...
Page 123: Ec Declaration Of Conformity In Accordance With The Pressure Equipment Directive
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 11 Appendix 11.6 EC declaration of conformity in accordance with the Pressure Equipment Directive Version 01/2011, Rev. 2.17 of 20/11/2024 • sb-bs 123 / 134 Translation of the original operating manual...
Page 124 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 11 Appendix Version 01/2011, Rev. 2.17 of 20/11/2024 • sb-bs 124 / 134 Translation of the original operating manual...
Page 125: Table Of The Sublimation Pressure Curve
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 11 Appendix 11.7 Table of the sublimation pressure curve °C = mbar °C = mbar °C = mbar °C = mbar 6,110 1,030 0,120 0,011 5,620 0,940 0,110 0,009 5,1770 0,850 0,100...
Page 126 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 11 Appendix Version 01/2011, Rev. 2.17 of 20/11/2024 • sb-bs 126 / 134 Translation of the original operating manual...
Page 127: 12 Glossary
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus 12 Glossary 12 Glossary Comparative pressure measurement During the sublimation, i.e. when the concentration of water vapour molecules is rather high in the atmosphere, the value that is provided by the gas-type dependent vacuum sensor of the "Pirani" type (e.g. Thyracont VCP 63) deviates from the value that is provided by a capacitive vacuum sensor (e.g.
Page 128 Wireless Shelf Technology With the Wireless Shelf Technology (WST), Martin Christ Gefrier- trocknungsanlagen GmbH has developed a system for laboratory freeze- dryers that works without cable connections at the electrically heated shelves and other equipment parts.
Page 129: 13 Index
Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus Index 13 Index Cleaning the freeze-dryer ......96 Click on touch ..........67 Access protection........67 equivalent ......... 107 Accessories ......13, 35, 96, 102 Commissioning .......... 13 Accident prevention ......9, 24, 26 Comparative pressure measurement ..
Page 130 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus Index Declaration of conformity (Machinery EC declaration of conformity (Pressure Directive) ........13, 117 Equipment Directive) ......123 Declaration of conformity (Pressure Editing a program........85 Equipment Directive) ......123 Electrical connection ........ 106 Declaration of conformity (UKCA) ....
Page 131 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus Index General malfunctions ......... 90 Important information ......... 23 General terms and conditions ....12 Infectious substances ......102 General work (maintenance) ...... 96 Informal safety notes ........26 Global warming potential (GWP) ....107 Initial start-up ..........
Page 132 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus Index Malfunctions ..........90 Option: Pressure increase test ..... 56, 82 Manual freeze-drying ......... 74 Option: USB port ........ 16, 113 Manufacturer ......96, 102, 106 Option: USB process recording ....55 Manufacturer contact data ......
Page 133 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus Index Solvent-containing products ....... 10 Solvents ............. 34 Radioactive substances ......97 Solvents (hazards) ........29 Rated current ..........16 Solvents............. 96 Reference designator ....57, 70, 128 Special equipment:water cooling system . 108 Refrigerant data .........
Page 134 Freeze-dryer Alpha 1-4 LSCplus Freeze-dryer Alpha 2-4 LSCplus Index Unit test ............. 60 Vacuum pump oil change ......72 Units of protection class I ......40 Vacuum pump warm-up ......79 USB port ..........16, 113 Vacuum pump, cleaning and care .... 100 USB process recorder ........

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