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Summary of Contents for Nikon ECLIPSE L200
- Page 1 M245 E 05.4.NF.4 IC Inspection Microscope ECLIPSE L200 / ECLIPSE L200D Instructions...
- Page 2 Thank you for purchasing the Nikon products. This instruction manual is written for the users of the Nikon’s IC inspection microscope “ECLIPSE L200 / ECLIPSE L200D” and describes the basic operations of the microscope. To ensure correct usage, read this manual carefully before operating the instrument.
- Page 3 Warning / Caution Symbols Used in This Manual Although Nikon products are designed to provide you with the utmost safety during use, incorrect usage or disregard of the instructions can cause personal injury or property damage. For your safety, read the instruction manual carefully and thoroughly before using the instrument.
- Page 4 Disassembly may cause malfunction, electrical shock and/or injury. Do not disassemble any part other than those described in this manual. If you experience any problem with the microscope, notify your nearest Nikon representative. Power cord. To prevent electrical shock, always turn off the power switch (flip it to the ○ side) before connecting or disconnecting the power cord.
- Page 5 Use only the specified lamp and the lamphouse on this microscope. The use of other lamps and lamphouses may lead to malfunction. • The specified lamphouse: 12V-100W halogen epi-lamphouse made by Nikon (model: Nikon LHS-H100P-2 HALOGEN 12V 100W) • The specified lamp:...
- Page 6 They may come off and may cause serious injury or malfunction. • Ask your nearest Nikon representative for the carrying rods of the microscope. • Before carrying the stage, attach the fixing metals to hold the movement of the stage plate.
- Page 7 LAYOUT DIAGRAMS 645 (Stage area) Center of gravity position 710 (Stage area) < Operator space > Center of Eye point gravity position Center of gravity position...
- Page 8 OPERATING POSTURE The figure below shows the operation posture that prevents strain on your body. Choose a workbench and a chair having the similar dimensions to those shown on the figure. The 95th percentile male (Height: 189.5 cm) (The front clearance for the knees) The 5th percentile female (Height: 147.5 cm)
- Page 9 viii...
- Page 10 CONTENTS Warning / Caution Symbols Used in This Manual ..........ii Meaning of Symbol Used on the Equipment ............ii WARNING ......................iii CAUTION ......................iv LAYOUT DIAGRAMS ....................vi OPERATING POSTURE ..................vii Nomenclature and Functions ..............1 Microscopy ....................4 1.
- Page 11 Nomenclature and Functions Names of the parts Analyzer slider * Eyepiece tube Polarizer slider * Lamphouse for episcopic Eyepieces illumination Pinhole slider * Arm of the microscope Main body of the microscope DIC slider * Revolving Breath shield plate nosepiece Objective Stage Lamphouse for...
- Page 12 Names of the operational parts Clamp for various adapters Optical path selection lever Analyzer slider * Polarizer slider * Diopter adjustment rings Pinhole slider * Pinhole centering Prism screws * movement knob Prism selection Bright/Dark-field knob illumination selection lever DIC slider * Coarse movement Aperture diaphragm lever ON switch...
- Page 13 Aperture diaphragm Filter sliders centering screw holes Focusing target lever Filter sliders Screw hole for carrying rod Coarse focus stopper ring Screw holes for AF drive Filter slots for diascopic illumination (on L200D only) “CAUTION for heat” symbol CAUTION label AF connector Input voltage indication Screw holes for carrying rods...
- Page 14 Microscopy In this chapter, each microscopy is described individually. Please also refer to the chapter “ Operation of Each Part” for how to operate each part. Episcopic bright-field microscopy ............P.5 Episcopic dark-field microscopy ............P.6 Episcopic DIC (differential interference contrast) microscopy ... P.7 Episcopic bright-field simplified polarization microscopy ....
- Page 15 Episcopic bright-field microscopy Set each part as follows. Press in the NCB and ND filters. Press in for 100% binocular observation. Slide out the analyzer and the polarizer. In the end, place the sample Press in for BF (bright-field). on the stage. Slide out the DIC slider.
- Page 16 Episcopic dark-field microscopy Mount the BD objectives. Note) Only the objectives marked “BD” can be used on the dark-field microscopy. BD marking on the objective ☞ Observe the sample with the episcopic bright-field microscopy. Switch to the episcopic dark-field microscopy. Adjust brightness with the ND filters.
- Page 17 Episcopic DIC (differential interference contrast) microscopy Attach the polarizer, analyzer and DIC sliders. The objective with “LU” marking is suitable for DIC microscopy. ☞ Observe the sample with the episcopic bright-field microscopy. Switch to the episcopic DIC microscopy. Adjust brightness with the ND filters.
- Page 18 Episcopic bright-field simplified polarization microscopy Attach the polarizer and analyzer. ☞ Observe the sample with the episcopic bright-field microscopy. Switch to the episcopic bright-field polarization microscopy. Adjust brightness with the ND filters. Press in the polarizer and the analyzer. Adjust brightness with the brightness control dial.
- Page 19 Diascopic bright-field microscopy (for L200D only) Set each part as follows. Press in for 100% binocular observation. Slide out the analyzer. In the end, place the Pull out for DF (dark-field). sample on the stage. Slide out the DIC slider. Lower the stage to the limit.
- Page 20 Operation of Each Part Filters There are four filter slots at the left side of the microscope arm. For L200D, there are two more filter slots in front of the lamphouse for diascopic illumination. The types and usage of the filters are as follows. Filters Usage Color balance adjustment for...
- Page 21 Adjusting the torque of the coarse focus knob To increase the torque The torque of the coarse focus knob can be adjusted. To increase the torque, turn the torque adjustment ring counter-clockwise. To reduce the torque, turn it clockwise, but please make sure that the stage does not fall on its own weight when you take your hands off the coarse focus knob.
- Page 22 Eyepiece tube Optical path selection The optical path selection lever can be used to switch the destination of light from the binocular part to the vertical tube. Push IN 100% 100% Pull OUT Tilting function The binocular part of the trinocular tilting eyepiece tube L2-TT can be swung up and down for finding the best postion for observation.
- Page 23 Diopter adjustment The diopter adjustment compensates for differences in eyesight between your left and right eyes. After the correct Outer tube adjustment, you will find the observation with both eyes edge easier and the focus shift is reduced when switched to the different objectives.
- Page 24 Aperture diaphragm About the aperture diaphragm • The aperture diaphragm adjusts the numerical aperture (N.A.) of the illumination system, and plays an important part in determining image resolution, contrast and depth of focus. • Generally, the aperture diaphragm is to be stopped down to 70 to 80% of the objective N.A. •...
- Page 25 8 x 8 Stage The 8 x 8 stage can be moved in either the “coarse” mode for swift and long ranged movement, or the “fine” mode for minute movement. To switch between the modes, use the coarse movement ON and OFF switches on the stage coarse movement lever.
- Page 26 Focusing target When you are to observe a sample with the polished surface under the episcopic illumination, the use of the focusing target will facilitate the focusing. How to use the focusing target. Pull out the focusing target lever to put the focusing target in the optical path.
- Page 27 Polarizer slider Use together with the analyzer slider for episcopic bright- field simplified polarization microscopy. Empty hole Use together with the analyzer and the DIC sliders for episcopic DIC microscopy. Attaching the polarizer slider Orientation Remove the cap near the indication “POLARIZER” at the adjustment screw right side of the microscope arm.
- Page 28 Analyzer slider Use together with the polarizer slider for episcopic bright- field simplified polarization microscopy. Analyzer Use together with the polarizer and the DIC sliders for Empty hole episcopic DIC microscopy. Attaching the analyzer slider Remove the cap near the indication “ANALYZER” at the right side of the microscope arm.
- Page 29 DIC slider Use together with the polarizer and the analyzer sliders for Prism movement knob episcopic DIC microscopy. Prism selection knob Attaching (removing) the DIC slider Use a hexagonal screwdriver to loosen the DIC slider limit screw on the revolving nosepiece. Insert the DIC slider into the slot on the nosepiece and screw in the DIC slider limit screw.
- Page 30 Pinhole slider When using the high magnification objectives, placing the Pinhole slider adapter pinhole on the optical path gives deep depth of focus to the image. This is especially effective when observing the contact holes. Attaching the pinhole slider Remove the cover plate near the indication “PINHOLE” at Pinhole the right side of the microscope arm.
- Page 31 Fiber adapter Attaching the fiber adapter instead of the episcopic Fiber clamp screw lamphouse will enable the user to use the illuminator with the fiber luminous section. Attaching the fiber adapter Loosen the lamphouse clamp screw to remove the episcopic lamphouse.
- Page 32 L200D EPI/DIA Simultaneous adapter If you are using L200D and wishes to turn on the EPI and DIA illumination at the same time, use the “L200D EPI/DIA simultaneous adapter”. L200D EPI/DIA Simultaneous adapter Attach the simultaneous adapter in between the diascopic lamphouse and the lamphouse mount at the rear bottom of the microscope main body and connect it to the Power Supply UN2 using the lamp cable supplied with the adapter.
- Page 33 If not the same, notify your nearest Nikon If not the same, do not use this power representative. supply.
- Page 34 Assembly Assemble each part of the microscope referring to the diagram on the next page. WARNING • Before assembling the microscope, be sure to read the WARNING and CAUTION at the beginning of this instruction manual and follow the instructions written therein. •...
- Page 35 Assembling the ECLIPSE L200 / L200D Eyepiece tube Eyepiece Analyzer slider Polarizer slider Pinhole slider ND, NCB, GIF filters Breath shield plate DIC slider Lamp and lamphouse Objective mount adapter Objective Objective (with ø25 mm (with ø32 mm screw) screw)
- Page 36 • To prevent burn injury, allow the lamp and the lamphouse to cool for at least 30 minutes after turning off the power switch, before replacing the lamp. • Use the “LHS-H100P-2 HALOGEN 12V100W” lamphouse manufactured by Nikon. • Use the 12V-100W LONGLIFE halogen lamp (OSRAM HLX 64623 or PHILIPS 7724).
- Page 37 Attaching the stage and the holder 1) Lower the substage to the limit with the coarse focus knob. 2) Place the stage on the substage and fix it by four M6 screws (supplied together with the substage) using the 5 mm hexagonal wrench.
- Page 38 Attaching the eyepiece tube Fully loosen the eyepiece tube fixing screw with the hexagonal screwdriver. Fit the eyepiece tube on to the mount on the top of the microscope arm and tighten the eyepiece tube clamp screw. When removing the eyepiece tube Take hold of the eyepiece tube when loosening the eyepiece tube clamp screw since the eyepiece tube may drop suddenly.
- Page 39 Connecting the power cord WARNING Use only the supplied power cord. Using the wrong power cord could result in damage or fire. (The specification of the supplied power cord is written below.) Also note that the protection Class 1 equipment should be connected to PE (protective earth) terminal.
- Page 40 Troubleshooting Improper use of the microscope may adversely affect its performance even though there is no damage on the microscope. If any of the problems listed below arise, take the countermeasures indicated. Viewing and control systems Troubles Causes Countermeasures Vignetteing in the Lamp is not installed correctly.
- Page 41 Troubles Causes Countermeasures Image is partially dim. Sample not securely fit on the stage. Fit the sample securely on the stage. Image moves while being focused. Stage tilted. Mount the stage correctly. (P.25) Image tinged yellow. NCB11 filter not used. Place the NCB11 filter in the optical path.
- Page 42 Troubles Causes Countermeasures Eye fatigue. Interpupillary adjustment not Adjust the interpupillary distance. correctly adjusted. (P.13) Eyepiece diopter not adjusted. Adjust the diopter. (P.13) Improper brightness. Adjust the brightness by the brightness control dial, or by placing ND filters in the optical path.
- Page 43 Electrical Troubles Causes Countermeasures Lamp does not light when No electricity. (Power cord not Connect the power cord. (P.27) switched on. connected.) No lamp installed. Install the lamp. (P.25) Lamp is blown. Replace the lamp. (P.25) Episcopic/Diascopic illumination Switch it correctly. selection switch not set correctly.
- Page 44 Before putting on the vinyl cover, turn off the power switch on the microscope (flip it to the ° side) and wait until the lamphouse is cool. Regular inspections Regular inspections of this microscope are recommended in order to maintain peak performance. Contact your nearest Nikon representative for details about regular inspections.
- Page 45 Electrical Specifications Input rating Input voltage: 100 to 240 V AC ±10%, 50/60 Hz Rated current: 2.4 A or less Built in fuse rating: 250 V, T4 A Power cord: Use only the following power supply cord. Using the wrong power cord could result in danger or fire.
- Page 46 Conforming standards • This model satisfies SEMI guidelines. • This model satisfies the UL standard. • This model satisfies FCC 15B Class A. This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment.
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