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Alltech ELSD 2000ES
Evaporative Light Scattering Detector
A l l t r a d e m a r k s , b r a n d n a m e s , a n d b r a n d s a p p e a r i n g h e r e i n a r e t h e p r o p e r t y o f t h e i r r e s p e c t i v e o w n e r s .
• C r i t i c a l a n d e x p e d i t e d s e r v i c e s
• I n s t o c k / R e a d y - t o - s h i p
Artisan Scientific Corporation dba Artisan Technology Group is not an affiliate, representative, or authorized distributor for any manufacturer listed herein.
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Used and in Excellent Condition
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https://www.artisantg.com/67283-1
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Table of Contents
Troubleshooting
Related Manuals for Alltech ELSD 2000ES
Summary of Contents for Alltech ELSD 2000ES
- Page 1 Alltech ELSD 2000ES Evaporative Light Scattering Detector Limited Availability Used and in Excellent Condition Open Web Page https://www.artisantg.com/67283-1 A l l t r a d e m a r k s , b r a n d n a m e s , a n d b r a n d s a p p e a r i n g h e r e i n a r e t h e p r o p e r t y o f t h e i r r e s p e c t i v e o w n e r s .
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Page 2: Table Of Contents
Table of Contents 1. INTRODUCTION About the ELSD 2000 ..........................4 What Is Included with the ELSD 2000 ....................4 Principle of Operation ..........................5 2. INSTALLATION What You Will Need ..........................6 Unpacking ............................... 6 Controls and Features ..........................7 2.3.1 Front Panel ............................ - Page 3 3.12 Configuration Options ..........................18 3.12.1 Set Audio Alarm .......................... 18 3.12.2 Select Full-Scale Voltage ......................18 3.12.3 Set Fault Relay Override ......................18 3.13 Diagnostics ............................18 4. ROUTINE OPERATION Safety ..............................18 Operation Notes ............................ 19 Important Operating Parameters ......................19 Selecting Initial Operating Conditions ....................
- Page 4 6. APPENDICES Specifications ............................36 Replacement Parts ..........................36 Contact Information ..........................36 Volatile Mobile Phase Modifiers ......................37 ELSD 2000 Cleaning Procedures ......................38 6.5.1 Drift Tube Cleaning Procedure ..................... 38 6.5.2 Nebulizer Cleaning Procedure ...................... 38 Power Module Adjustments ........................39 6.6.1 Changing the Input Voltage ......................
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Page 5: Introduction
The ELSD 2000 shipping container should contain the 1.1 ABOUT THE ELSD 2000 following: The Alltech ELSD 2000 (Evaporative Light Scattering Detector) is designed for use with High Performance Liquid • ELSD 2000 Detector Chromatography (HPLC) systems to analyze any compound •... -
Page 6: Principle Of Operation
1.3 PRINCIPLE OF OPERATION Lower mobile phase flowrates require lower gas flowrates for proper nebulization. Substitution of a 2.1mm ID column for The unique detection principle of evaporative light scattering your standard 4.6mm ID column will allow you to greatly detectors involves nebulization of the column effluent to form reduce the mobile phase flowrate while also increasing the an aerosol, followed by solvent evaporation in a heated drift... -
Page 7: Installation
Use only the distributor or to Alltech Associates. Please refer to Section exhaust trap kit provided with the detector. 6.7, Warranty, Returns, and Repairs, for more information. -
Page 8: Controls And Features
2.3 CONTROLS AND FEATURES OUTPUT mV METHOD: A Read 30.0 °C Tube Temp: 30.0 Gas Flow: 2.0 L/min Gain: Impactor: 1000mV/FS [Edit] [Menu] [Standby] [Zero] Figure 2.1 2.3.1 FRONT PANEL (Figure 2.1) All instrument parameters for the ELSD 2000 can be displayed and controlled through the front panel: 2. -
Page 9: Right Side Panel
The fuse value will depend on the line voltage (6A for 120V, 3A for 240V). 2. Twelve-Pin Connector: Outputs TTL/contact closure Alltech Associates, Inc. 2051 Waukegan Rd. Deerfield, IL 60015 signals or accepts signals from peripheral equipment. Only pins 1 through 7 currently have programmed functions. -
Page 10: Making Electrical And Fluid Connections
AllChrom Plus software (sold separately, must be free of contaminants, such as oil, water, particulates, contact Alltech for more information). or any other non-volatile substances. A 0.1µm gas filter is built into the instrument. 4. Exhaust Outlet: Nebulizer gas, mobile phase vapor, and solute mist or particles produced during an analysis 4. - Page 11 7. Signal Output: Connect a signal cable from your data CAUTION: The liquid level in the drain container must collection device to the OUTPUT port on the rear panel of the be monitored during Impactor ‘On’ mode, and excess detector. liquid should be removed when the level approaches the top of the container.
- Page 12 9. 12-Pin Connections: Make connections to the 12-pin terminal strip on the rear panel of the detector depending on which of the following functions are needed: Remote Gas Control: Pin 1: Ground (-) Pin 2: Signal (+) Pins 1 and 2 on the ELSD 2000 can accept a TTL/contact closure signal to turn gas flow on/off.
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Page 13: Instrument Control
3. INSTRUMENT CONTROL 3.1 ELSD 2000 CONTROL OPTIONS The ELSD 2000 may be controlled either directly through the front panel or remotely using a PC. NOTE: Only the ELSD 2000 PC Control software included with the unit or AllChrom ™ Plus software can be used for PC control of the ELSD 2000. -
Page 14: Powering Up
In this mode, the laser, gas flow, and drift tube heaters are turned off. The Operation screen will display a Alltech Associates, Inc. ‘Standby’ message with a timer indicating how long the instrument has been in this state. Screen key options are [Menu] and [Run]. -
Page 15: Menu Screen
3.7 MENU SCREEN 3.8 SETTING UP A RUN The <<Menu>> screen is accessed through the Operation To set up a run you will need to: screen by pressing the [Menu] screen key. It offers the 1. Load the desired method. following options: Choose from the following method options: <<Menu>>... -
Page 16: Method Screen
3.9 METHOD SCREEN 3.10 METHOD OPTIONS Press 1 from the <<Menu>> screen to bring up the 3.10.1 SELECT A PREVIOUSLY SAVED METHOD <<Method>> screen. It offers the following options: Press 1 from the <<Method>> screen to choose the Select Method option. This will bring up the <<Method List>> <<Method>>... -
Page 17: Edit An Existing Method
3.10.2 EDIT AN EXISTING METHOD 3. Tube Temperature: Press 3 to change the drift tube temperature, and the current setpoint will flash. Enter the Methods are edited through the <<Edit Method>> screen. new temperature setting using the numeric keys. To access this screen: allowable drift tube temperature range is 25°... -
Page 18: Create A New Method
Cancelling Method Changes 3.11 CONFIGURATION SCREEN Press [Cancel] from the <<Edit Method>> screen to cancel Press 2 from the <<Menu>> screen to access the ALL method changes made in the current editing session, <<Configuration>> screen. and you will return to the <<Menu>> screen with the original <<Configuration>>... -
Page 19: Configuration Options
5. DO NOT REMOVE THE COVER of the instrument the 10mV and 1000mV full-scale voltage settings until you unless instructed to you by an Alltech representative. reach the desired setting. The correct setting will depend on the data collection system that is used. -
Page 20: Operation Notes
4.2 OPERATION NOTES 4.3 IMPORTANT OPERATING PARAMETERS 1. Monitor the liquid level in the 500mL drain waste Ideal performance of the ELSD 2000 is obtained by choosing container during Impactor ‘On’ mode and remove excess the best settings for an application. Nebulizer gas flowrate, liquid when necessary. -
Page 21: Mobile Phase Flowrate
Impactor ‘On’: The gas flowrates used for Impactor ‘On’ 4. DRIFT TUBE TEMPERATURE: The proper drift tube mode will generally be lower than flowrates used for Impactor temperature setting will be based on mobile phase volatility ‘Off’ mode. Gas flowrates will usually be about 2.2L/min or and flowrate, nebulizer gas flowrate, and analyte volatility. -
Page 22: Selecting Initial Operating Conditions
4.4 SELECTING INITIAL OPERATING Higher or Lower Mobile Phase Flowrates CONDITIONS The recommended starting conditions in Table 1 are for mobile phase flowrates of 1.0mL/min with a standard 4.6mm To select initial operating conditions you must: ID column. Lower mobile phase flowrates and smaller bore columns may require much lower gas flowrates and drift tube 1. -
Page 23: Selecting Initial Conditions For Impactor 'On' Mode
4.4.2 SELECTING INITIAL CONDITIONS FOR IMPACTOR ‘ON’ MODE Impactor ‘On’ applications require a different approach. Use Table 2 below as a guide for selecting initial conditions for this impactor setting: INITIAL ELSD OPERATING CONDITIONS: IMPACTOR ‘ON’ MODE Analyte Mobile Phase Drift Tube Composition Composition... -
Page 24: Start-Up Sequence
4.5 START-UP SEQUENCE 11. After flushing the column, connect it to the LIQUID INLET on the left side panel of the detector. The tubing between 1. Set up the unit as described in Section 2.4, Making the column and the nebulizer should be as short as Electrical and Fluid Connections. -
Page 25: Shutdown Sequence
4.6 SHUTDOWN SEQUENCE 4.7 OPTIMIZATION PROCEDURE 1. Turn off the mobile phase flow. Follow the optimization procedure based on the impactor setting used for your application: 2. Allow gas only to flow for approximately five minutes to clear any remaining droplets. 4.7.1 OPTIMIZATION FOR IMPACTOR ‘OFF’... -
Page 26: Optimization For Impactor 'On' Mode
Gas Flowrate 4.7.2 OPTIMIZATION FOR IMPACTOR ‘ON’ MODE Impactor ‘On’ applications require a different approach for 1. Set the drift tube to the optimal temperature determined optimization than Impactor ‘Off’. from the procedure above. 2. Set the gas flowrate to the value recommended in Drift Tube Temperature/Gas Flowrate Table 1. -
Page 27: Diagnostics And Troubleshooting
65-80psi. Nebulizer may be blocked. Clean nebulizer. Contact Alltech for details. Possible gas flow sensor error Contact Alltech if error or electronics error. persists. Inlet gas Inlet gas pressure is lower than Regulator pressure setting Adjust regulator pressure pressure is low. -
Page 28: Diagnostics Options
5.2 DIAGNOSTICS OPTIONS 5.3.1 OPTICS TEST 1. Turn off mobile phase flow. Wait several minutes for Several diagnostic functions are available for troubleshooting system to equilibrate with only gas flowing. purposes. Press 3 on the <<Menu>> screen to bring up the <<Diagnostics>>... -
Page 29: Nebulizer Gas Pressure Test
2. Turn off mobile phase flow. Wait several minutes for high. the unit to stabilize. Laser on Optics and/or Contact Alltech for 3. Press 2 from the <<Diagnostic Tests>> screen to start offset is too light trap may cleaning details. -
Page 30: Flow Meter Test
0.2L/min during the testing period. is unstable. gas source and remedy if Example screen with pass results: necessary. Gas flow meter error. Contact Alltech. Test passed. Press any key to continue. Nebulizer may need Contact Alltech. Current Max. Min. -
Page 31: Communication Test
1. Short out pins 2 and 3 on the RS-232 port on the rear empty. and replace if panel of the unit. Contact Alltech if you need assistance. necessary. 2. Press 4 on the <<Diagnostic Tests>> screen to perform Gas leaks present Contact Alltech. -
Page 32: Manual Control Screen
5.4 MANUAL CONTROL SCREEN 5.5 ERROR LOG The <<Manual Control>> screen is helpful during equilibration The Error Log is useful for tracking errors that occur during and troubleshooting. Press 2 from the <<Diagnostics>> operation of the instrument. Press 3 from the <<Diagnostics>> screen to bring up the <<Manual Control>>... -
Page 33: Diagnosing Baseline Noise
2. The nebulizer, drift tube, and/or optics may need cleaning. • Mobile phase off Refer to Sections 6.5 for drift tube and cleaning • Nebulizing gas on procedures. Contact Alltech for details on cleaning optics. • Laser on Result: • Noise disappears when gas is turned off. -
Page 34: Troubleshooting Charts
Replace with fresh, filtered, higher-quality quality material. mobile phase. Drift tube and/or optical cell dirty. Refer to Section 6.5.1 for drift tube cleaning procedure. Contact Alltech for details on cleaning optics. Improper nebulization. Nebulizer may be partially obstructed. Refer to Section 6.5.2 for nebulizer cleaning procedure. - Page 35 Clean nebulizer. Refer to Section 6.5.2 for nebulizer cleaning details. Source gas pressure too low. Adjust source pressure to between 65-80psi. Clogged inlet gas filter. Replace filter. Contact Alltech. Gas source may be low or empty. Check gas source. Replace if needed. No power Line unplugged.
- Page 36 Examine the interior of the drift tube with a flashlight to check impactor position. Contact Alltech if position does not match setpoint. Gain set too low. Increase gain value. Autosampler needle not pulling up sample Repair or replace equipment as needed.
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Page 37: Specifications
Windows ™ based PC control 6.3 CONTACT INFORMATION Power Requirements: 120/240V, 50/60Hz Alltech Associates, Inc. 2051 Waukegan Road Dimensions: 23.0" H x 12.5" W x 21.6" D Deerfield, IL 60015 USA (58.4cm H x 31.8cm W x Phone: (847) 948-8600 54.8cm D) -
Page 38: Volatile Mobile Phase Modifiers
6.4 VOLATILE MOBILE PHASE MODIFIERS VOLATILE BUFFERS AND MOBILE PHASE MODIFIERS pH Range Acids Trifluoroacetic Acid 13.70 72.4°C Formic Acid 3.75 10.25 100.7°C Acetic Acid 4.75 9.25 116.0°C Carbonic Acid 6.37 7.63 — Bases Ammonia 9.25 4.75 -33.35°C Methylamine 10.81 3.19 16.6°C Ethylamine... -
Page 39: Elsd 2000 Cleaning Procedures
8. Resume the normal use of the detector. If the problem persists, continue troubleshooting with the manual or persists, continue troubleshooting with the manual or contact Alltech. contact Alltech. 6.5.2 NEBULIZER CLEANING PROCEDURE The nebulizer may require occasional cleaning due to particulate blockage, either of the gas or liquid inlets. -
Page 40: Power Module Adjustments
6.6.2 FUSE REPLACEMENT 6.6 POWER MODULE ADJUSTMENTS 1. Turn the detector power off and unplug the power 6.6.1 CHANGING THE INPUT VOLTAGE cord. 1. Power off the detector and unplug the power cord 2. Slide the fuse drawer out. See Figure 6.1. from the rear panel of the detector. -
Page 41: Warranty, Returns, And Repairs
Alltech from other manufacturers are limited to RETURNS the guarantees given on such equipment by the respective If it is necessary to return any material to Alltech, please call manufacturers. Alltech’s Customer Service Department for a Return Authorization Number and forwarding instructions. No returns Any modifications made to equipment covered by this may be made without a Return Authorization Number. -
Page 42: Useful References
Effects of the Nature of the Solvent and Solutes on the 6.8 USEFUL REFERENCES Response of a Light Scattering Detector, Michel Righezza, Journal of Liquid Chromatography , 11(9 & 10), 1967-2004 Theory and Reviews (1988). Dual-Mode ELSD: Optimum Performance for a Wide Range of Applications, A. - Page 43 Analysis of Lipoxygenase Kinetics by High-Performance Lipid Applications Liquid Chromatography with a Polymer Column, A. Nunez Estimation of Free Glycolipids in Wheat Flour by HPLC, and G.J. Piazza, Lipids , 30(2), 129-133 (1995). J.B. Ohm, and O.K. Chung, Cereal Chemistry , 76(6), 873- 876 (1999).
- Page 44 Separation of Neutral Lipids by High-Performance Liquid Pharmaceutical Applications Chromatography: Quantification by Ultraviolet, Light Determination of Fumonisins B , and B by High- Scattering and Fluorescence Detection, E. J. Murphy, T. Performance Liquid Chromatography with Evaporative A. Rosenberger, and Lloyd A. Horrocks, Journal of Light-Scattering Detection, J.G.
- Page 45 Acrylic Block Copolymer Analysis by Adsorption Chromatography with ELSD, B.L. Neff, H.J. Spinelli, Journal of Applied Polymer Science , 42, 595-600 (1991). Use of an Evaporative Light Scattering Detector in Reversed-Phase High-Performance Liquid Chrom- atography of Oligomeric Surfactants, Y. Mengerink, H.C. De Man, S.J.
- Page 46 ALLTECH ELSD 2000 EVAPORATIVE LIGHT SCATTERING DETECTOR OPERATING MANUAL April, 2001 Manual Part No. 600100M Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
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