Table of Contents
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1.1
About the ELSD 2000 ............................................................................................................................. 4
1.2
What Is Included with the ELSD 2000 .................................................................................................... 4
1.3
Principle of Operation ............................................................................................................................. 5
2.1
What You Will Need ................................................................................................................................ 6
2.2
Unpacking ............................................................................................................................................... 6
2.3
Controls and Features ............................................................................................................................ 7
2.3.1 Front Panel .................................................................................................................................... 7
2.3.2 Left Side Panel .............................................................................................................................. 7
2.3.3 Right Side Panel ............................................................................................................................ 8
2.3.4 Rear Panel ..................................................................................................................................... 8
2.4
Making Electrical and Fluid Connections ................................................................................................ 9
3.1 ELSD 2000 Control Options ................................................................................................................. 12
3.2 Using the ELSD 2000 Control Panel .................................................................................................... 12
3.3 Navigating the Screens ......................................................................................................................... 12
3.4 Powering Up ......................................................................................................................................... 13
3.5 Operation Screen .................................................................................................................................. 13
3.6 Instrument Status .................................................................................................................................. 13
3.6.1 Standby ........................................................................................................................................ 13
3.6.2 Run .............................................................................................................................................. 13
3.7 Menu Screen ........................................................................................................................................ 14
3.8 Setting Up a Run .................................................................................................................................. 14
3.9 Method Screen ..................................................................................................................................... 15
3.10 Method Options .................................................................................................................................... 15
3.10.1 Select a Previously Saved Method ............................................................................................ 15
3.10.2 Edit an Existing Method ............................................................................................................. 16
3.10.3 Create a New Method ................................................................................................................ 17
3.11 Configuration Screen ............................................................................................................................ 17
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Summary of Contents for Alltech ELSD 2000
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Page 1: Table Of Contents
Table of Contents 1. INTRODUCTION About the ELSD 2000 ..........................4 What Is Included with the ELSD 2000 ....................4 Principle of Operation ..........................5 2. INSTALLATION What You Will Need ..........................6 Unpacking ............................... 6 Controls and Features ..........................7 2.3.1 Front Panel ............................ - Page 2 3.12 Configuration Options ..........................18 3.12.1 Set Audio Alarm .......................... 18 3.12.2 Select Full-Scale Voltage ......................18 3.12.3 Set Fault Relay Override ......................18 3.13 Diagnostics ............................18 4. ROUTINE OPERATION Safety ..............................18 Operation Notes ............................ 19 Important Operating Parameters ......................19 Selecting Initial Operating Conditions ....................
- Page 3 Specifications ............................36 Replacement Parts ..........................36 Contact Information ..........................36 Volatile Mobile Phase Modifiers ......................37 ELSD 2000 Cleaning Procedures ......................38 6.5.1 Drift Tube Cleaning Procedure ..................... 38 6.5.2 Nebulizer Cleaning Procedure ...................... 38 Power Module Adjustments ........................39 6.6.1 Changing the Input Voltage ......................
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Page 4: Introduction
• Exhaust Trap Kit: a chromophore or fluorophore for detection. Exhaust Tubing Collection Flask with Stopper, 500mL The ELSD 2000 offers the most advanced evaporative light Exhaust Elbow scattering detection technology available. The nebulizer has Lead Ring been redesigned for increased ruggedness. Digital gas flow •... -
Page 5: Principle Of Operation
Evaporation In order to provide optimum sensitivity for all applications, the ELSD 2000 is designed to operate in two different modes (patent pending). Depending on the application, the Teflon ® coated stainless steel impactor is placed either parallel (Impactor ‘Off’... -
Page 6: Installation
Exhaust System: The ELSD 2000 has been carefully shipped to ensure that it • A fume hood or other ventilation device located is received in proper condition. Any damage to the container or its contents should be reported immediately to your local close to the detector to remove the detector exhaust from the laboratory. -
Page 7: Controls And Features
[Standby] [Zero] Figure 2.1 2.3.1 FRONT PANEL (Figure 2.1) All instrument parameters for the ELSD 2000 can be displayed and controlled through the front panel: 2. Numeric Keypad: Membrane-based keypad provides 1. LCD (Liquid Crystal Display): The main screen displayed values 0 through 9, ‘... -
Page 8: Right Side Panel
The fuse value will depend on the line voltage (6A for 120V, 3A for 240V). 2. Twelve-Pin Connector: Outputs TTL/contact closure Alltech Associates, Inc. 2051 Waukegan Rd. Deerfield, IL 60015 signals or accepts signals from peripheral equipment. Only pins 1 through 7 currently have programmed functions. -
Page 9: Making Electrical And Fluid Connections
3. RS-232 Port: An RS-232 cable is connected to this port supply should be regulated from 65 to 80 psig. This inlet for PC control of the ELSD 2000. PC control will also require accepts a 1/8” CPI Parker fitting. A stable gas flow and the use of either the ELSD 2000 Control Software included pressure are necessary for reproducible results. - Page 10 7. Signal Output: Connect a signal cable from your data CAUTION: The liquid level in the drain container must collection device to the OUTPUT port on the rear panel of the be monitored during Impactor ‘On’ mode, and excess detector. liquid should be removed when the level approaches the top of the container.
- Page 11 Pin 6: Common (-) Pin 7: Normally Closed (NC) (+) Pins 5, 6, and 7 on the ELSD 2000 can output a TTL/contact closure signal to stop pump flow whenever an operation error occurs on the detector. Consult the appropriate manuals for wiring details.
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Page 12: Instrument Control
This section of the manual describes operation of the ELSD 2000 directly through the front control panel. For details on PC control of the ELSD 2000, please consult the ELSD 2000 Control Software Manual for instructions. 3.2 USING THE ELSD 2000 CONTROL PANEL... -
Page 13: Powering Up
3.4 POWERING UP 3.6 INSTRUMENT STATUS The ELSD 2000 is powered up using the power switch on the The ELSD 2000 has two different operational states: rear panel of the instrument. A Welcome screen will appear ‘Standby’ and ‘Run’. Current status of the instrument is... -
Page 14: Menu Screen
3.7 MENU SCREEN 3.8 SETTING UP A RUN The <<Menu>> screen is accessed through the Operation To set up a run you will need to: screen by pressing the [Menu] screen key. It offers the 1. Load the desired method. following options: Choose from the following method options: <<Menu>>... -
Page 15: Method Screen
3.9 METHOD SCREEN 3.10 METHOD OPTIONS 3.10.1 SELECT A PREVIOUSLY SAVED METHOD Press 1 from the <<Menu>> screen to bring up the <<Method>> screen. It offers the following options: Press 1 from the <<Method>> screen to choose the Select Method option. This will bring up the <<Method List>> <<Method>>... -
Page 16: Edit An Existing Method
3.10.2 EDIT AN EXISTING METHOD 3. Tube Temperature: Press 3 to change the drift tube temperature, and the current setpoint will flash. Enter the Methods are edited through the <<Edit Method>> screen. new temperature setting using the numeric keys. To access this screen: allowable drift tube temperature range is 25°... -
Page 17: Create A New Method
Cancelling Method Changes 3.11 CONFIGURATION SCREEN Press [Cancel] from the <<Edit Method>> screen to cancel Press 2 from the <<Menu>> screen to access the ALL method changes made in the current editing session, <<Configuration>> screen. and you will return to the <<Menu>> screen with the original <<Configuration>>... -
Page 18: Configuration Options
5. DO NOT REMOVE THE COVER of the instrument the 10mV and 1000mV full-scale voltage settings until you unless instructed to you by an Alltech representative. reach the desired setting. The correct setting will depend on the data collection system that is used. -
Page 19: Operation Notes
4.3 IMPORTANT OPERATING PARAMETERS 1. Monitor the liquid level in the 500mL drain waste Ideal performance of the ELSD 2000 is obtained by choosing container during Impactor ‘On’ mode and remove excess the best settings for an application. Nebulizer gas flowrate, liquid when necessary. - Page 20 Impactor ‘On’: The gas flowrates used for Impactor ‘On’ 4. DRIFT TUBE TEMPERATURE: The proper drift tube mode will generally be lower than flowrates used for Impactor temperature setting will be based on mobile phase volatility ‘Off’ mode. Gas flowrates will usually be about 2.2L/min or and flowrate, nebulizer gas flowrate, and analyte volatility.
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Page 21: Selecting Initial Operating Conditions
4.4 SELECTING INITIAL OPERATING Higher or Lower Mobile Phase Flowrates CONDITIONS The recommended starting conditions in Table 1 are for mobile phase flowrates of 1.0mL/min with a standard 4.6mm To select initial operating conditions you must: ID column. Lower mobile phase flowrates and smaller bore columns may require much lower gas flowrates and drift tube 1. -
Page 22: Selecting Initial Conditions For Impactor 'On' Mode
4.4.2 SELECTING INITIAL CONDITIONS FOR IMPACTOR ‘ON’ MODE Impactor ‘On’ applications require a different approach. Use Table 2 below as a guide for selecting initial conditions for this impactor setting: INITIAL ELSD OPERATING CONDITIONS: IMPACTOR ‘ON’ MODE Analyte Mobile Phase Drift Tube Composition Composition... -
Page 23: Start-Up Sequence
15. You are now ready to inject your samples. The first 8. If the baseline is unstable and/or drifting, the unit may time you use the ELSD 2000, you may want to reproduce have not reached its temperature setpoint. In the Impactor the ELSD 2000 QC procedure listed in the performance ‘On’... -
Page 24: Shutdown Sequence
4.6 SHUTDOWN SEQUENCE 4.7 OPTIMIZATION PROCEDURE 1. Turn off the mobile phase flow. Follow the optimization procedure based on the impactor setting used for your application: 2. Allow gas only to flow for approximately five minutes to clear any remaining droplets. 4.7.1 OPTIMIZATION FOR IMPACTOR ‘OFF’... -
Page 25: Optimization For Impactor 'On' Mode
Gas Flowrate 4.7.2 OPTIMIZATION FOR IMPACTOR ‘ON’ MODE Impactor ‘On’ applications require a different approach for 1. Set the drift tube to the optimal temperature determined optimization than Impactor ‘Off’. from the procedure above. 2. Set the gas flowrate to the value recommended in Drift Tube Temperature/Gas Flowrate Table 1. -
Page 26: Diagnostics And Troubleshooting
65-80psi. Nebulizer may be blocked. Clean nebulizer. Contact Alltech for details. Possible gas flow sensor error Contact Alltech if error or electronics error. persists. Inlet gas Inlet gas pressure is lower than Regulator pressure setting Adjust regulator pressure pressure is low. -
Page 27: Diagnostics Options
5.3.1 OPTICS TEST 5.2 DIAGNOSTICS OPTIONS 1. Turn off mobile phase flow. Wait several minutes for Several diagnostic functions are available for troubleshooting system to equilibrate with only gas flowing. purposes. Press 3 on the <<Menu>> screen to bring up the <<Diagnostics>>... -
Page 28: Nebulizer Gas Pressure Test
2. Turn off mobile phase flow. Wait several minutes for high. the unit to stabilize. Laser on Optics and/or Contact Alltech for 3. Press 2 from the <<Diagnostic Tests>> screen to start offset is too light trap may cleaning details. -
Page 29: Flow Meter Test
0.2L/min during the testing period. is unstable. gas source and remedy if Example screen with pass results: necessary. Gas flow meter error. Contact Alltech. Test passed. Press any key to continue. Nebulizer may need Contact Alltech. Current Max. Min. -
Page 30: Communication Test
1. Short out pins 2 and 3 on the RS-232 port on the rear empty. and replace if panel of the unit. Contact Alltech if you need assistance. necessary. 2. Press 4 on the <<Diagnostic Tests>> screen to perform Gas leaks present Contact Alltech. -
Page 31: Manual Control Screen
5.4 MANUAL CONTROL SCREEN 5.5 ERROR LOG The <<Manual Control>> screen is helpful during equilibration The Error Log is useful for tracking errors that occur during and troubleshooting. Press 2 from the <<Diagnostics>> operation of the instrument. Press 3 from the <<Diagnostics>> screen to bring up the <<Manual Control>>... -
Page 32: Diagnosing Baseline Noise
2. The nebulizer, drift tube, and/or optics may need cleaning. • Mobile phase off Refer to Sections 6.5 for drift tube and cleaning • Nebulizing gas on procedures. Contact Alltech for details on cleaning optics. • Laser on Result: • Noise disappears when gas is turned off. -
Page 33: Troubleshooting Charts
Replace with fresh, filtered, higher-quality quality material. mobile phase. Drift tube and/or optical cell dirty. Refer to Section 6.5.1 for drift tube cleaning procedure. Contact Alltech for details on cleaning optics. Improper nebulization. Nebulizer may be partially obstructed. Refer to Section 6.5.2 for nebulizer cleaning procedure. - Page 34 Clean nebulizer. Refer to Section 6.5.2 for nebulizer cleaning details. Source gas pressure too low. Adjust source pressure to between 65-80psi. Clogged inlet gas filter. Replace filter. Contact Alltech. Gas source may be low or empty. Check gas source. Replace if needed. No power Line unplugged.
- Page 35 Examine the interior of the drift tube with a flashlight to check impactor position. Contact Alltech if position does not match setpoint. Gain set too low. Increase gain value. Autosampler needle not pulling up sample Repair or replace equipment as needed.
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Page 36: Specifications
Outputs: Contact closure-fault 272106 RS-232 Cable relay; Serial (RS-232) I/O for ELSD 2000 Windows ™ based PC control 600101M ELSD 2000 PC Control Operating Manual Operating Parameter Selection & Display: Graphical LCD with alphanumeric keypad or ™ Windows based PC control 6.3 CONTACT INFORMATION... -
Page 37: Volatile Mobile Phase Modifiers
6.4 VOLATILE MOBILE PHASE MODIFIERS VOLATILE BUFFERS AND MOBILE PHASE MODIFIERS pH Range Acids Trifluoroacetic Acid 13.70 72.4°C Formic Acid 3.75 10.25 100.7°C Acetic Acid 4.75 9.25 116.0°C Carbonic Acid 6.37 7.63 — Bases Ammonia 9.25 4.75 -33.35°C Methylamine 10.81 3.19 16.6°C Ethylamine... -
Page 38: Elsd 2000 Cleaning Procedures
6.5 ELSD 2000 Cleaning Procedures 5. To easily access the nebulizer it may be necessary to remove the front panel. There are 4 inside screws on 6.5.1 DRIFT TUBE CLEANING PROCEDURE each side that hold the front panel to the instrument chassis. -
Page 39: Power Module Adjustments
6.6.2 FUSE REPLACEMENT 6.6 POWER MODULE ADJUSTMENTS 1. Turn the detector power off and unplug the power 6.6.1 CHANGING THE INPUT VOLTAGE cord. 1. Power off the detector and unplug the power cord 2. Slide the fuse drawer out. See Figure 6.1. from the rear panel of the detector. -
Page 40: Warranty, Returns, And Repairs
Alltech from other manufacturers are limited to RETURNS the guarantees given on such equipment by the respective If it is necessary to return any material to Alltech, please call manufacturers. Alltech’s Customer Service Department for a Return Authorization Number and forwarding instructions. No returns Any modifications made to equipment covered by this may be made without a Return Authorization Number. -
Page 41: Useful References
Effects of the Nature of the Solvent and Solutes on the 6.8 USEFUL REFERENCES Response of a Light Scattering Detector, Michel Righezza, Journal of Liquid Chromatography , 11(9 & 10), 1967-2004 Theory and Reviews (1988). Dual-Mode ELSD: Optimum Performance for a Wide Range of Applications, A. - Page 42 Lipid Applications Analysis of Lipoxygenase Kinetics by High-Performance Liquid Chromatography with a Polymer Column, A. Nunez Estimation of Free Glycolipids in Wheat Flour by HPLC, and G.J. Piazza, Lipids , 30(2), 129-133 (1995). J.B. Ohm, and O.K. Chung, Cereal Chemistry , 76(6), 873- 876 (1999).
- Page 43 Separation of Neutral Lipids by High-Performance Liquid Pharmaceutical Applications Chromatography: Quantification by Ultraviolet, Light Determination of Fumonisins B , and B by High- Scattering and Fluorescence Detection, E. J. Murphy, T. Performance Liquid Chromatography with Evaporative A. Rosenberger, and Lloyd A. Horrocks, Journal of Light-Scattering Detection, J.G.
- Page 44 Acrylic Block Copolymer Analysis by Adsorption Chromatography with ELSD, B.L. Neff, H.J. Spinelli, Journal of Applied Polymer Science , 42, 595-600 (1991). Use of an Evaporative Light Scattering Detector in Reversed-Phase High-Performance Liquid Chrom- atography of Oligomeric Surfactants, Y. Mengerink, H.C. De Man, S.J.
- Page 45 ALLTECH ELSD 2000 EVAPORATIVE LIGHT SCATTERING DETECTOR OPERATING MANUAL April, 2001 Manual Part No. 600100M...
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