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Summary of Contents for Smith-Root eDNA Sampler
- Page 1 INSTRUCTION MANUAL For eDNA Sampler videos and tutorials, visit: www. smith-root.com/support/tutorials 11569.04 eDNA Sampler Instruction Manual...
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Page 2: Table Of Contents
Items manufactured by companies other than Smith-Root carry the original manufacturer’s warranty. Please contact product manufacturer for return instructions. All Smith-Root, Inc. manufactured products are covered by a one year warranty. Credit & Refund Policy: Customers returning equipment, in new condition, will be given credit five days from the date of the return. -
Page 3: Edna Sampler Unit
2. Transect sampling pole for mobile sampling (sold separately) 3. Single-use filter packets for each sampling location (purchased separately) 4. Long tubing with inline strainer for eDNA Sampler “IN” port 5. Short tubing for eDNA Sampler “OUT” port 6. Remote control for eDNA Sampler pump 7. -
Page 4: Warnings
INSTRUCTION MANUAL A. Control/display panel I. Input power cable B. USB connector J. Battery (standalone sampler) C. Speaker/Volume K. Harness* D. Power Switch L. Upper dry bag* E. Water outlet M. Lower dry bag* F. Water inlet N. Waterproof door Left and middle: stand-alone sampler;... -
Page 5: Battery Installation
2) the inline strainer positioned approximately 3 inches (~8cm) from the intake port of the pump (see picture). Please do not operate the eDNA Sampler without the inline strainer in place. Occasionally the strainer may become clogged with particulate and prevent water flow, in which case the strainer will need to be replaced with a new one. -
Page 6: Edna Sampler Interface
INSTRUCTION MANUAL eDNA SAMPLER INTERFACE 1. Menu button 2. Item select buttons 3. Back button 4. Target Volume – The target sample volume in liters (L) 5. Target Flow – The flow rate set point in liters per minute (L/min) 6. -
Page 7: Auto Mode
SAMPLER AUTO MODE When the system is in auto mode, the user will be alerted by 2 beeps indicating that the filter should be inverted to achieve the target sample volume. The system will continue to meter the water con- tained in hose and filter housing even after the noz- zle is removed from water. -
Page 8: Menu Screen
INSTRUCTION MANUAL MENU SCREEN 1. Maximum Pressure – The maximum pressure in negative Pounds per Square Inch (PSI) 2. Minimum Flow – Flow rate at which the Low Flow Alarm will sound (5 rapid beeps) 3. Volume Offset – Value deducted from target volume to alarm user when to invert filter 4. -
Page 9: Volume Offset
SAMPLER VOLUME OFFSET This value needs to be changed whenever the tubing length or filter arrangement is altered (for example, switching from 1 filter housing to 3 on the transect pole). To calcu- late the volume offset for the current setup,... -
Page 10: Point Sampling Procedure With Telescoping Pole
5. Connect other long tubing end to the eDNA Sampler unit “IN” port on the right- hand side (if facing display). 6. Connect short length of tubing to the “OUT” port on the left-hand side of unit. - Page 11 SAMPLER 10. Extend the telescoping pole to the needed length to reach your sampling location. 11. Power on the remote control using the ON/OFF button. 12. Select between Auto and Manual modes. Use Auto mode when targeting a specific sample volume.
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Page 12: Determining A Realistic Sample Volume
INSTRUCTION MANUAL 1. System beeps twice 2. Rotate quickly to invert 3. Elevate filter housing 4. Retract pole to remove filter DETERMINING A REALISTIC SAMPLE VOLUME If you are working in a new environment and the filterable water volume is not known, the low flow alarm can be used as an objective measure of filter clogging. - Page 13 SAMPLER 4. Press the Reset button on the interface to start a new data record. 5. Connect a filter of a given pore size (e.g. 1.2 um) to the pump tubing. 6. Start the pump and begin filtration such as the procedure described in the Point Sampling section.
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Page 14: Sample Preservation
3. Label the sample with an ID and place the preserved sample in a storage box. Samples can then be stored in the dry bags of the eDNA Sampler unit. 4. Record the Log ID from the interface associated with the sample ID. -
Page 15: Instructions For Self-Preserving Filters
Note: Self-preserving filter housings are currently rated to 10 PSI max pressure (20 in.-Hg; 508 mm-Hg). Please program your eDNA Sampler for a 10 PSI pressure threshold. 1. Open a sample packet containing a 47mm self-preserving filter housing and attach the extension tube to the housing. - Page 16 STATEMENT OF BIODEGRADATION Smith-Root self-preserving filter housings contain a material that is recognized as one of very few synthetic polymers which are water soluble and “inherently” biodegradable in water. The polymer manufacturer’s internal experimental results indicate it is “inherently” biode- gradable in water when the degree of hydrolysis is higher than 70 mol%.
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Page 17: Transect Sampling Procedure
– thus allowing for standardization of volume filtered per unit distance. For mobile sampling we suggest using the Smith-Root Transect pole (Part # 11203); this allows for up to 3 filter housing to be run simultaneously. -
Page 18: Estimate Your Transect Duration
2. If the environment will require you stop and start the pump repeatedly to avoid obstructions or shallow areas, program the eDNA Sampler to Manual mode. In Auto mode the data record will be reset each time you press Start. - Page 19 SAMPLER 1. When an obstruction is encountered, press the Start/Stop button to stop the pump, but leave the extension tubes in the water. 2. Quickly rotate the pole and invert the filter housings, retaining as much water in the extension tubes as possible.
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Page 20: Completing The Transect
MARINE APPLICATIONS The eDNA Sampler can be used in salt water, however, a fresh water rinse is required after each use. Please follow the storage procedure to flush the system. -
Page 21: Maintenance
SAMPLER MAINTENANCE • Before each use inspect the equipment for wear or damage and replace worn or damaged parts as necessary. • Inspect the batteries for damage. Do not use a damaged battery, replace it immediately. • Follow the charging procedures to maintain the batteries. -
Page 22: Sterilization
1. Always replace the orange “IN” and “OUT” port caps when the system is not in use. 2. When you return from sampling, or if the EDNA Sampler unit will not be in use for > 24 hours, please use the following procedure: a. -
Page 23: Obtaining Service
SAMPLER OBTAINING SERVICE For additional assistance with your eDNA Sampler system, or for repairs, please contact Smith-Root at 360-573-0202 or visit www.smith-root.com. TIPS AND TRICKS FOR LONG TERM OPERATION • Follow the pump storage procedure guidelines (do not store dry for prolonged periods). -
Page 24: Appendix
SAMPLER APPENDIX SPECIFICATIONS* BATTERIES Main battery: 9.6 Ahr 12.8V LiFePO4, Model K2B12V19EB Remote battery: AA 1900 mAHr 1.2V NiMH x 2, Model BK-3MC- Runtime Main Battery: 6-8 Hrs Runtime Remote Battery: ~ 4 Hrs Main Battery Terminals Polarized latching slide type. - Page 25 SAMPLER APPENDIX Minimum flow rate: 0.1 l/min Maximum flow rate: 1.4 l/min Maximum measurable volume: 999.9 l Volume accuracy: > 90% at 0.1 l/min or higher flow GPS system compatibility: GNSS (GPS, Galileo, GLONASS, BeiDou) Number of channels: GPS, GLONASS sensitivity:...
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Page 26: Adjusting The Packframe
SAMPLER APPENDIX PACKFRAME ADJUSTMENT For maximum stability and comfort, adjustment points should be tightened enough that the backpack doesn’t move inde- pendently of your body while walking. When worn correctly, the pack frame should be upright when you are standing;... - Page 27 It may be necessary in some circumstances to remove CAUTION: Always the eDNA Sampler backpack quickly. The unit includes an unlatch the sternum strap integral quick release pack frame. By pressing the latch of the pack before enter- tabs on the waist belt and shoulder straps, the entire unit ing or crossing water.
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Page 28: Batteries
CAUTION: Failure to use this equipment in a manner consistent with the guidance pro- vided in this manual may reduce the protection provided by this equipment. The eDNA Sampler uses a 12 volt sealed deep cycle battery. Understanding the proper care of this battery will reduce problems in the field. -
Page 29: Battery Storage Instructions
3 to 5 years depending on ambient temperature, depth of discharge (D.O.D.), and number of cycles (for Smith-Root batteries to maintain at least 80% of original capacity, they are rated 230 cycles for 100% D.O.D., 470 cycles for 50% D.O.D. or 1100 cycles for 30% D.O.D.). -
Page 30: Battery Rating
SAMPLER APPENDIX BATTERY RATING (The following deals with lead acid batteries) Bat- Life Load Capacity teries are rated at the current which will reduce 20 hr 0.60A 12.0Ah the voltage per cell to 1.67 volts in 20 hours. The 10 hr 1.05A... -
Page 31: Service Life
SAMPLER APPENDIX LITHIUM IRON PHOSPHATE BATTERIES ADVANTAGES: movement that can cause a short circuit. • Much lighter than lead-acid batteries. Shipping of these batteries requires special • Longer service life, i.e. more charge/dis- package labeling that may vary slightly from charge cycles. - Page 32 Recharging with overnight or over a weekend. This may result the Smith-Root supplied lithium battery char- in over-charging of the battery and possible ger is automatic and consists of two stages: damage to the battery and reduced battery life.
- Page 33 All batteries are subject to disposal and recycling regulations that very by country and region. Always check and follow your appli- cable regulations before disposing of any battery. Contact your local battery recycling organization or return to Smith-Root for recy- cling. www.smith-root.com...
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Page 34: Unit Decals
SAMPLER APPENDIX UNIT DECALS SMITH ROOT, INC. • www.smith-root.com Input/Entrée: 3V DC 400mA IP:54 Made in the U.S.A. / Fabriqué aux États-Unis. Contains FCC ID: MCQ-XBEE3: This device complies with part 15 of the FCC rules. Operation is subject to the following two conditions: (i.) this device may not cause harmful... - Page 36 (360) 573-0202 Vancouver, WA USA www.smith-root.com...
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