Contents Of Shipping Container; Matrix Software Overview - revvity CELLOMETER ASCEND User Manual

WARNING: Use of controls or adjustments or performance of procedures other than those specified
herein or by Revvity, Inc. may result in a hazardous process.

CONTENTS OF SHIPPING CONTAINER

Cellometer Ascend Instrument
Power Supply and Power Cord
Matrix Software (pre-installed on instrument)
USB Drive containing PDF files listed below:
− 8004707 Cellometer Matrix Software User Manual
− 8004708 Cellometer Ascend User Manual (this document)
− 8004709 Cellometer Ascend Quick Start Guide
− 8004710 Cellometer Ascend Focus Guide
Box of 100 3-Chamber Counting Chamber Slides (ASD-CHM3-001)
Five 8-Chamber Counting Slides (not for individual sale; ASD-CHM8-001 for a box of 100)
One 0.5 mL vial of Fluorescent Counting Beads (not for individual sale; CCBM-015-0.5mL)
One 1.0 mL vial of AO/PI Viability Reagent (not for individual sale; CS2-0106-5mL for a 5 mL vial)

MATRIX SOFTWARE OVERVIEW

The Matrix software used to run the Cellometer Ascend is shared by multiple product families and includes default
assays (identified with ASD_, K2_, MX_, or PLX_instrument prefixes) and report templates for all supported product
types. However, only assays available for the Ascend will be displayed when entering setup details, and report
templates will be adjusted dynamically for presentation based on scan results. Assays and report templates for all
product types can be viewed in the Manage tab > Assays and Report Templates screens.
Key elements in Matrix software functionality are described below:
• Acquiring Sample Data − The Acquire tab > Setup and Preview options are sequential screens used to acquire
sample data. After loading samples, entering setup details, selecting at least one well and then tapping the
Preview button, you can preview live channel images and adjust instrument focus/fluorescent exposure for
each channel. Tapping the Count button engages the camera to acquire sample images as specified by the
selected assay, which are then processed by the Matrix imaging and pattern-recognition software to decluster,
identify and count individual cells according to defined cell type parameters.
Analyzing Scan Results − The Data tab > Select, Results and Recount options are sequential screens used to
•
analyze scan results. Once a result is selected, you can vary the image displayed by tapping available channel
buttons (e.g., BR, FL1, FL2, etc.) appearing across the top of the Well View tab or by choosing another well.
Data associated with the well appears below the image or can be viewed as a consolidated report including
data from all wells by tapping the Summary tab. To fine-tune the analysis of a scan, tap the Recount button
and either select a new assay or tap the View button for the current assay to edit parameters as necessary. Last
Used Assay parameters are provided for your reference.
8004708 Cellometer™ Ascend User Manual Rev A
Chapter 1. Introduction
USB Drive
Ascend Slides
2