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Summary of Contents for revvity CELLOMETER ASCEND
- Page 1 U S E R M A N U A L CELLOMETER™ ASCEND FLUORESCENT CELL COUNTER 8004708 Rev A For Research Use Only. Not for use in diagnostic procedures.
- Page 2 – without the written permission of Revvity, Inc. While every precaution has been taken in the preparation of this document, Revvity assumes no responsibility for errors or omissions, or for damages resulting in the use of information contained in this document or from the use of programs and source code that may accompany it.
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Page 3: Table Of Contents
Table of Contents CHAPTER 1. INTRODUCTION ....................1 Cellometer Ascend Instrument Overview ....................1 Intended Use ............................... 1 Contents of Shipping Container ....................... 2 Matrix Software Overview ........................2 Matrix 21 CFR Part 11 Module Overview ....................3 About this User Manual ..........................4 Glossary of Abbreviations ......................... - Page 4 Table of Contents CHAPTER 5. OPERATION ......................19 Launching the Software .......................... 19 Matrix Screen Elements .......................... 19 Navigation Bar ..............................20 Workflow Tabs ..............................20 Simplified Workflow ............................22 User Favorites................................. 22 Streamlined User Experience ............................22 Focus Methods ..............................23 Image-Based Auto Focus ..............................
- Page 5 CHAPTER 10. TROUBLESHOOTING AND FAQS ................ 47 Troubleshooting and Instrument Messages ................... 47 Frequently Asked Questions ........................48 CHAPTER 11. REVVITY SUPPORT .................... 49 Scope of Support Services ........................49 Contact Methods ............................ 49 Reporting an Issue to Support ........................... 49 Generating Diagnostic Reports .......................
- Page 6 Table of Contents 8004708 Cellometer™ Ascend User Manual Rev A...
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Page 7: Chapter 1. Introduction
Intended Use Intended use of the Cellometer Ascend is to count cell lines and primary cells (e.g., total nucleated cells, PBMCs, splenocytes, hepatocytes, stem cells, tumor/tissue digests, etc.) with the ability to determine cell count, viability and additional analysis using trypan blue exclusion or fluorescent reagents. -
Page 8: Contents Of Shipping Container
One 1.0 mL vial of AO/PI Viability Reagent (not for individual sale; CS2-0106-5mL for a 5 mL vial) MATRIX SOFTWARE OVERVIEW The Matrix software used to run the Cellometer Ascend is shared by multiple product families and includes default assays (identified with ASD_, K2_, MX_, or PLX_instrument prefixes) and report templates for all supported product types. -
Page 9: Matrix 21 Cfr Part 11 Module Overview
Chapter 1. Introduction Setting Up Auto Exports − The Matrix auto export feature allows you to select the Images (e.g., Raw Images and • Colorized Images), Data (e.g., Well Level CSV, Object Level CSV and Object Level ACS) and Archive (e.g., Data Set) output file types to be exported for an assay after completion of scan analysis. -
Page 10: About This User Manual
Chapter 1. Introduction ABOUT THIS USER MANUAL This Cellometer Ascend Matrix User Manual provides information on the following topics: Instrument and Software Overviews Matrix Counting and Analysis Workflow • • • Potential Hazards and Safety Information • Cleaning, Maintenance and Storage •... -
Page 11: Glossary Of Abbreviations
Chapter 1. Introduction GLOSSARY OF ABBREVIATIONS The following abbreviations may be displayed on the shipping container, on the Cellometer Ascend device label or in this user manual. Amperes Kilowatt Alternating Current Pound American National Standards Institute Personal Computer ANSI Acridine Orange... -
Page 12: Glossary Of Symbols
Chapter 1. Introduction GLOSSARY OF SYMBOLS The following international symbols may be displayed on the shipping container, on the Cellometer Ascend device label or in this user manual. Fragile, Handle with Care. Keep Dry. Located on This End Up. Located on... -
Page 13: Chapter 2. Hazards, Safety, And Environment
Note: The Cellometer Ascend instrument should not be used adjacent to or stacked with other equipment unless specified by Revvity. If the system must be used adjacent to or stacked with other equipment, then observe the instrument in its configuration to verify instrument operation is normal and functions as expected. -
Page 14: Instrument System Hazard
CellC-support@revvity.com Servicing Hazard No one other than Revvity-authorized personnel may service inside the protective cover of the Cellometer Ascend instrument. CAUTION: Do not pull the system by the connectors. Toppling of the system or causing damage to the system may result in the instrument no longer functioning as expected. -
Page 15: Safety Information
Revvity assumes no liability whatsoever for any damage, loss or injury resulting from an application of a product that is not in strict accordance with the instructions provided with the product. Revvity also assumes no liability for any damage or injury arising due to operator error or mistake, including, but not limited to, injury arising from operator’s lack of qualification or as a result of errors or mistakes committed by such operator. -
Page 16: Environmental Requirements
Chapter 2. Hazards, Safety and Environment ENVIRONMENTAL REQUIREMENTS Environmental requirements for intended operation of the Cellometer Ascend instrument are presented below. For Indoor Use Only − − Elevation: 0 to 2,000 m − Temperature Range: 10 °C to 30 °C −... -
Page 17: Chapter 3. Instrument Description
Chapter 3. Instrument Description This chapter describes the Cellometer Ascend instrument components, device label and system specifications. ASCEND COMPONENTS Sample Slot: Counting chamber slides are inserted into this slot for viewing (accepts only one slide at a time). Ports: One port on front of instrument (USB A). -
Page 18: Instrument Specifications
Chapter 3. Instrument Description INSTRUMENT SPECIFICATIONS The Cellometer Ascend is your all-in-one automated cell viability counter. Using both Brightfield and Dual- Fluorescent imaging in combination with pattern-recognition software, it quickly and accurately identifies and counts cells. For cultured cells stained with trypan blue or fluorescent reagents, the Ascend simultaneously calculates Live/Dead and Total cell counts, Viability (%), and Live and Total cell concentrations, including a helpful graphic overlay used to highlight live and dead cells with color-coded outlines in the image being viewed. -
Page 19: Technical Information
DECLARATION OF CONFORMITY Cellometer Ascend conforms to appropriate country standards and governing regulations as listed in the Declaration of Conformity. To request the Ascend Declaration of Conformity, contact Support by visiting https://www.revvity.com/contact-us or send an email to: CellC-support@revvity.com... - Page 20 Chapter 3. Instrument Description 8004708 Cellometer™ Ascend User Manual Rev A...
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Page 21: Chapter 4. Unpacking And Site Preparation
Cellometer Ascend (see Facility Requirements on page 16). Our staff would be happy to help you set up your Cellometer Ascend. For assistance, contact Support by visiting https://www.revvity.com/contact-us or send an email to: CellC-support@revvity.com 1. -
Page 22: Site Preparation
Due to how the instrument is used with liquids, the instrument should be placed on a level surface. Keep the area around the Cellometer Ascend clean between, during and post operation. Electrical requirements for the Cellometer Ascend are as follows: •... -
Page 23: Instrument And Software Validation
Revvity, Inc. has designed an Installation Qualification/Operation Qualification (IQ/OQ) validation specifically for the Cellometer Ascend. Our experienced Support team is available to assist your organization during set up of the instrument and while performing the IQ/OQ validation. For more information, contact Support by visiting https://www.revvity.com/contact-us or send an email to: CellC-support@revvity.com... - Page 24 Chapter 4. Unpacking and Site Preparation 8004708 Cellometer™ Ascend User Manual Rev A...
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Page 25: Chapter 5. Operation
Chapter 5. Operation This chapter describes launching the Matrix software, presents basic screen elements, and provides details on how to view the installed software version. As the Ascend is a touch-screen device, tap gently on screen elements (e.g., tabs, icons, dropdowns, or buttons) using a finger or stylus to make software graphical user interface (GUI) selections. -
Page 26: Navigation Bar
Chapter 5. Operation Note: This instrument is a touch-screen device enabling users to interact directly with GUI by tapping gently on screen elements (e.g., tabs, icons, dropdowns, or buttons) using a finger or stylus pen. As an alternative, a USB mouse may be connected. - Page 27 Chapter 5. Operation Note: The Skip Preview field selection is used to toggle the display of the Preview (i.e., Skip Preview is set to No) and Count (i.e., Skip Preview is set to Yes) buttons. Data Tab: Selects the Data Analysis workflow. You must first select a scan result to view its count results.
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Page 28: Simplified Workflow
Chapter 5. Operation Simplified Workflow Simplified workflow features have been integrated into the Matrix software graphical user interface (GUI) to streamline user input in the most common data acquisition and analysis workflows. USER FAVORITES A key feature introduced in the v6.0 release is the creation of user favorites. A Favorite is a collection of count parameters such as an assay, consumable name/type, and reports/exports settings that can be saved for quick re- use. -
Page 29: Focus Methods
SLIDE-BASED AUTO FOCUS Available for the Cellometer Ascend for the 3-Chamber Slide Only. The Slide-based auto focusing operation allows the software/instrument to focus on the consumable (without needing to focus directly on the sample) by using a target manufactured on the slide. -
Page 30: Viewing Software Version
Chapter 5. Operation VIEWING SOFTWARE VERSION The Home tab displays the logo for the instrument to which you are connected and the Matrix software logo. It also contains the About Matrix™, Generate Diagnostic Report, and Clear All Logs buttons. Tap the About Matrix™ button to display product information (e.g., instrument type and software version), Support contact information, software details (e.g., build number, database server/name), and instrument details (e.g., instrument firmware version and instrument serial number/camera serial number). -
Page 31: Chapter 6. Viability Methods
Note: Cell concentrations of 2.0 x 10 − 2.0 x 10 cells/mL can be analyzed on Cellometer Ascend, with a concentration of 2.0 x 10 cells/mL being optimal. If comparing the Trypan Blue and AO/PI viability methods, a portion of the sample should be stained with trypan blue and another portion stained with AO/PI. -
Page 32: Using Ao/Pi Viability Method
In images captured by Cellometer Ascend using the AO/PI viability method, red blood cells (RBCs, marked by red arrows) seen in the brightfield image (on left) are not seen in the fluorescent image (in middle), but are clearly identified in the overlay image (on right). -
Page 33: Ao/Pi Staining Solution Guidelines
Chapter 6. Viability Methods The table below shows the recommended dilution when preparing cell samples for AO/PI viability analysis and the final Dilution Factor to enter into the Matrix software for a variety of sample types. Preliminary Sample Volume of Volume of Final Dilution Sample Type... - Page 34 Chapter 6. Viability Methods 8004708 Cellometer™ Ascend User Manual Rev A...
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Page 35: Chapter 7. Sample Preparation
Note that Ascend 3-Chamber slides have already been labeled with A, B, and C. LOADING SAMPLES INTO COUNTING CHAMBERS Cellometer Ascend 3-Chamber and 8-Chamber consumable slides consist of independent chambers manufactured to a precisely controlled height. A cell suspension of 20 µL is pipetted into a chamber through its inlet port. - Page 36 Chapter 7. Sample Preparation Load a counting chamber by touching the pipette tip to the chamber’s inlet port and slowly pipetting the entire recommended sample volume per chamber all at once. Capillary force automatically spreads the sample within the chamber. Each counting chamber can hold a separate sample as there is no mixing between the two individual chambers on a slide.
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Page 37: Chapter 8. Matrix Counting And Analysis Workflow
Chapter 8. Matrix Counting and Analysis Workflow This chapter presents the basic workflow for counting cells using the Cellometer Ascend powered by Matrix software, including best practices and tips. For a complete reference on using Matrix software, see the Cellometer Matrix Software User Manual for details. -
Page 38: Entering Parameter Settings
Chapter 8. Matrix Counting and Analysis Workflow ENTERING PARAMETER SETTINGS 1. In the General area, enter a Consumable ID. If a consumable ID is not entered, a date/time stamp will be appended to the “New Sample” default (e.g., New Sample 2024/03/25-10:58:09). 2. -
Page 39: Loading Samples
Chapter 8. Matrix Counting and Analysis Workflow In addition, Exports (see descriptions provided below) and output files for Reports (e.g., CSV, Excel, PDF, and Word) defined for the current assay are displayed. Sample Exports include: Raw Images Represents Black and White high-resolution PNG images for each channel in the assay. Colorized Images Represents colorized, high-resolution PNG images of all acquired channels. -
Page 40: Preview Mode
Chapter 8. Matrix Counting and Analysis Workflow Preview Mode If Preview mode is enabled (i.e., Skip Preview field in Setup screen is set to No), the instrument engages its camera for viewing samples and displays the Preview screen. Users can view live images of samples in selected wells, preview available channels for Imaging Mode associated with the assay, adjust instrument focus and confirm fluorescent exposure for each channel. -
Page 41: Previewing Channel Images
Chapter 8. Matrix Counting and Analysis Workflow PREVIEWING CHANNEL IMAGES In the Preview area, channels available for viewing (e.g., Channel 1 and Channel 2) are based on the assay Imaging Mode. The Channel 1/BR image is displayed by default. Tapping the FL button displays the Channel 1/FL image. Note: If only one channel is available, the Preview field channel dropdown will be hidden. -
Page 42: Count Mode
Chapter 8. Matrix Counting and Analysis Workflow Count Mode Once you have completed previewing the live image for the sample, tap the Count button located at the bottom of the Preview screen. The instrument camera acquires sample images as specified by the selected assay which are then used by the Matrix Software to calculate count results according to defined cell type parameters. -
Page 43: Analyzing Scan Results
Chapter 8. Matrix Counting and Analysis Workflow ANALYZING SCAN RESULTS When analyzing scan results, an image is displayed in the viewing pane for a specific counting chamber and area. To vary the image displayed, users can tap different chambers (in the Consumable area) and different areas (in the slide visualization), or tap inside the viewing pane to drag and drop the image. -
Page 44: Changing Well View Image Display
Chapter 8. Matrix Counting and Analysis Workflow Changing Well View Image Display The Well View tab will always be displayed in the Results screen and includes both an image and the associated count results for the selected “well” or chamber. If multiple images were taken (based on your Images Per Well selection), they will each represent a separate area in the chamber (e.g., 4 Images Per Well will result in an image of each quadrant). -
Page 45: Exporting/Printing Scan Result Data
Chapter 8. Matrix Counting and Analysis Workflow For example, a custom Growth Curve report tab has been enabled in the scan result shown below. Tapping this tab displays data in the format defined by the associated report template (i.e., illustrating calculation of a growth curve for a time course series of scan results). -
Page 46: Exporting And Printing Scan Results (Manual Settings)
Chapter 8. Matrix Counting and Analysis Workflow Exporting and Printing Scan Results (Manual Settings) To manually select additional export options (images/data) for scan result files as well as generated output files for reports, tap the Export button located at the bottom of the Results screen. The Export dialog is displayed. Confirm the export Location and if necessary update this path by tapping the Browse button, navigating to a folder on your Operating Computer or network, and tapping OK. -
Page 47: Performing A Recount
Chapter 8. Matrix Counting and Analysis Workflow PERFORMING A RECOUNT If you find it necessary to fine-tune assay parameters after reviewing your data results, tap the Recount button located at the bottom of the Results screen. The Recount screen is displayed. Once you have made any necessary changes (per the options described below), tap the Recount button located at the bottom of the screen. -
Page 48: Modifying Auto Export Options
Chapter 8. Matrix Counting and Analysis Workflow If you drag indicators from Recount Channels to their home Scan Channels locations, a red outline is used to highlight empty Recount Channels locations. All Recount Channels must contain an indicator prior to recount. Modifying Auto Export Options Expand the Reports and Exports area to identify a Location for automatic exports of images/data and generated output files for reports. -
Page 49: Best Practices And Workflow Tips
Note: If you need help optimizing assay and/or cell type parameter settings, contact Support by visiting https://www.revvity.com/contact-us or send an email to: CellC-support@revvity.com When exporting scan results, the Matrix software creates a hierarchical folder structure (by default) in the •... - Page 50 Chapter 8. Matrix Counting and Analysis Workflow Data analysis features such as a Cell Size Histogram and Sample Adjustment Calculator can easily be built into • report templates to facilitate and enhance results visualization and evaluation. See the Cellometer Matrix Software User Manual for details on editing report templates and how to add them as tabs to customize assay scan results.
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Page 51: Chapter 9. Cleaning, Maintenance And Storage
Chapter 9. Cleaning, Maintenance and Storage Keeping the Cellometer Ascend and its operative area clean between runs, during use and post runs is a best practice and prevents contamination. Caring for the instrument and its consumables is also a best practice. -
Page 52: Storage
The warranty will immediately become void should unauthorized personnel attempt to repair or modify the instrument. To schedule all preventive maintenance needs and address any functionality concerns, contact Support by visiting https://www.revvity.com/contact-us or send an email to: CellC-support@revvity.com STORAGE When preparing the instrument for storage: •... -
Page 53: Chapter 10. Troubleshooting And Faqs
In cases where this issue is not resolved by the procedure above or if it continues to happen on a regular basis, contact Support by visiting https://www.revvity.com/contact-us or send an email to: CellC-support@revvity.com 8004708 Cellometer™ Ascend User Manual Rev A... -
Page 54: Frequently Asked Questions
Chapter 10. Troubleshooting and FAQs FREQUENTLY ASKED QUESTIONS How do I order a replacement for an instrument Power Cord that has been lost? Contact Support with the serial number of the instrument to request a replacement power cord/cable. When testing for cell viability, why are my count results lower than expected? When preparing a sample to test for cell viability, ensure that the stock solution is being used as indicated in Trypan Blue Staining Solution Guidelines on page 25 or AO/PI Staining Solution Guidelines on page 27. -
Page 55: Chapter 11. Revvity Support
Image Cytometry product for support. Enter instrument serial number, name and model, your contact details, and a detailed description to report the issue to Support. Visit https://www.revvity.com/contact-us-by-phone to find the global phone number for your area. • Send an email to CellC-support@revvity.com •... -
Page 56: Generating Diagnostic Reports
Files in the zipped folder include logs (located in C:\logs\Matrix\vXXX where XXX represents installed version) and configuration files (located in C:\ProgramData\Revvity\Matrix\vXXX\Configurations). 4. Attach the zipped folder to an email, include the Support Ticket ID (if assigned) in the Subject line and send to: CellC-support@revvity.com... -
Page 57: Appendix A. Consumables
• • No potential cross-contamination Visit the Cellometer and Cellaca Slides page on our website for a current listing or contact your Revvity Sales representative and to purchase Cellometer counting chamber slides directly from Revvity, Inc. ASSAY REAGENTS AND KITS Revvity provides a variety of assay reagents and reagent kits to accurately perform fluorescence-based cell counting and viability assays, including measuring percent viability and the number of live/dead cells. - Page 58 Appendix A. Consumables 8004708 Cellometer™ Ascend User Manual Rev A...
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Page 59: Appendix B. Warranty And License Details
(including unauthorized upgrades), disclose it to third parties or use it for any purpose other than running your Cellometer system. Revvity does not grant you any other rights to use or disclose the original Cellometer Image Cytometer software, Matrix software or subsequent upgrades, and any further uses will be prosecuted by Revvity, Inc. -
Page 60: Limitation Of Liability (Hardware And Software)
Buyer shall have no right in or to the Revvity Proprietary Information. Buyer will not disclose the Revvity Proprietary Information to any third party or use it in any manner outside the scope of the license including: (1) developing, designing, manufacturing, engineering, reverse engineering, refurbishing, selling or offering for sale items, parts or components of items, derivatives of or equivalents, or (2) assisting any third party in any manner to perform such activity. - Page 61 Revvity Health Sciences Inc. 360 Merrimack Street, Suite 200 Lawrence, MA 01843 USA...
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