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Emulate Zoe-CM2 User Manual

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Zoë-CM2
TM
User Guide

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Summary of Contents for Emulate Zoe-CM2

  • Page 1 Zoë-CM2 User Guide...
  • Page 3 Zoë Culture Module ® Zoë means life. Zoë-CM2 Culture Module is designed to sustain the life of cells within Organ-Chips. The instrument automates the precise conditions needed for simultaneous cell culture of up to 12 Organ-Chips. It supplies the dynamic flow of media and recreates the mechanical forces of breathing motions or peristalsis that help Organ-Chips recreate the microenvironment cells experience in the body.
  • Page 4 Zoë Culture Module Table of Contents Introduction Specifications Safety Page 6 Page 13 Page 15 Page 18 Introduction + Use Zoë-CM2 Culture Module / Table of Contents Page 4...
  • Page 5 Zoë Culture Module Table of Contents Settings Updating Firmware & Help & Care Downloading Logs Support Page 33 Page 35 Page 38 Page 39 Settings + Support Zoë-CM2 Culture Module / Table of Contents Page 5...
  • Page 6 Introduction This section provides an overview of Zoë and its functions. Equipment (what’s in the box) Zoë-CM2 Culture Module Chip Cradles Gas & Vacuum Supply Line Zoë Core Update Cable SensorPush HT1 Temperature and Humidity Smart Sensor Metal Spudger Pry Tool 24V Power Supply Page 6...
  • Page 7 Zoë-CM2 Culture Module Power Switch – Used to power Components the device on and off. Dial – Used for selecting 1. Zoë-CM2 Culture Module elements on the display, basic navigation, and adjusting values. 2. Power Switch Dial Button – Used to confirm 3.
  • Page 8 Organ-Chip Configuration 1. Bottom Channel Inlet Port 2. Top Channel Inlet Port 3. Vacuum Ports 4. Top Channel Indicator 5. Top Channel Outlet Port 6. Bottom Channel Outlet Port 7. Chip Body 8. Top Channel Indicator Zoë-CM2 Culture Module / Introduction Page 8...
  • Page 9 Configuration 1. Pod Reservoir Lid 2. Pod Reservoir 3. Organ-Chip 4. Chip Carrier Zoë-CM2 Culture Module / Introduction Page 9...
  • Page 10 Components 1. Top Channel Inlet 2. Top Channel Outlet 3. Vacuum Chamber 4. Bottom Channel Outlet 5. Bottom Channel Inlet Zoë-CM2 Culture Module / Introduction Page 10...

  • Page 11: Items For Installation

    Zoë-CM2 Items for Installation Please reach out to Emulate Support for detailed installation guidance. Zoë-CM2 comes with the following items for installation: Power Cord Power Supply Zoë-CM2 Resource Bundle (Gas & Vacuum Supply Line) Zoë-CM2 Culture Module / Installation Page 11...
  • Page 12 Zoë-CM2 Items for Installation The user will need to provide the following equipment for installation: Incubator 100% CO Gas Supply Orb-HM1 Note: Contact Emulate for guidance on user-provided equipment. Zoë-CM2 Culture Module / Installation Page 12...

  • Page 13: Specifications

    Specifications This section provides specific technical information and operational requirements. Included are system dimensions, thermal and gas requirements, and modes of operation. Page 13...
  • Page 14 Gas Input Pressure: 276 kPa +/- 35 (40 psig +/- 5) Gas Input Composition: 5% CO , balance air Vacuum Input Pressure: -70 kPa (-10.2 psig) Manufacturer Emulate, Inc. 27 Drydock Avenue Environmental Operating Temperature: 20–38ºC (68-100. 4ºF) Boston, MA 02210 Relative Humidity: 0–95%...
  • Page 15 Safety This section includes recommended precautions when handling and using Zoë-CM2. The information here describes how users can minimize the chance of harming themselves or the module during operation. Page 15...
  • Page 16 Zoë-CM2 Safety The Instructions Symbol The product is marked with this warning symbol where it is necessary for the user to refer to the instructions in the user guides. Regulatory Compliance and Testing This product has been tested to the requirements of: 61010-1 (IEC, EN, UL, CSA) IEC 61010-2-10 Safety Requirements for Electrical Equipment for measurement, control, and laboratory use...

  • Page 17: Caution Statements

    Service and maintenance should only be completed by Emulate- Never attempt to maintain or sanitize Zoë without first disconnecting certified personnel. Never attempt to disassemble or repair Zoë without from the power source.
  • Page 18 This section describes proper interaction with, and operation of, Zoë-CM2. The instrument should always be used in an incubator while following aseptic procedures. Page 18...

  • Page 19: Getting Started

    Zoë-CM2 Getting Started Zoë-CM2 Interface Display Dial Dial button Bay Activation Buttons Powering On To power Zoë on, push the Power Switch located at the rear of the system where the gas and power connections are located. Zoë will then go through its startup routine until it displays the status screen. Note: For stable operation, please keep the Zoë...
  • Page 20 Zoë-CM2 Running the Prime Cycle The Prime Cycle perfuses media through the Pod before it is connected to a seeded chip to ensure no air is trapped when a chip is connected to the Pod. Prepare Pods per desired organ-specific protocol. Insert Tray with up to six Pods into a Zoë...
  • Page 21 Zoë-CM2 Running the Prime Cycle Press the Dial Button to select “Start,” which A status bar and timer will appear to show the will initiate the Prime Cycle. This will cause the progress of the Prime Cycle. manifolds to lower. Zoë-CM2 Culture Module / Use Page 21...
  • Page 22 Zoë-CM2 Running the Prime Cycle Once the Prime Cycle is complete, the status bar Remove the primed Pods from Zoë and attach the and timer will disappear, the manifolds will rise, prepared chips to the Pods. Reference organ- and the Bay(s) will be deactivated. specific protocols for instructions on chip preparation prior to attachment.

  • Page 23: Selecting Media Type

    Zoë-CM2 Press the Dial Button to edit the “Media” type. Rotate the Dial to select between Fluid and Air/Gel: Selecting Media Type Using the Dial, hover over the areas below “Media” denoting the “Top” and “Bottom” channels. Zoë-CM2 Culture Module / Use Page 23...
  • Page 24 Zoë-CM2 Selecting Media Type These settings will now take effect on Zoë. After hovering over the desired media type, press the Dial Button to confirm. Note: If selecting Air/Gel, the flow rate will automatically be set to 0 to prevent media flow, as there is no flow in the channel when “Air/Gel”...
  • Page 25 Zoë-CM2 Press the Dial Button to begin editing flow rate. Rotate the Dial to adjust the flow rate between 10 and 1,000 µL / hr. Programming Flow Rate Using the Dial, hover over the areas below “Rate” denoting the “Top” and “Bottom” channels. Zoë-CM2 Culture Module / Use Page 25...
  • Page 26 Zoë-CM2 Programming Flow Rate After rotating to the desired flow rate, press the These settings will now take effect on Zoë. Dial Button to confirm. Zoë-CM2 Culture Module / Use Page 26...
  • Page 27 Zoë-CM2 Press the Dial Button to begin editing the strain. Rotate the Dial to adjust the level of strain between 0% and 12%. Programming Stretch Parameters Using the Dial, hover over the “Strain” parameter. This option allows you to set the amount of stretch on the chips.
  • Page 28 Zoë-CM2 Programming Stretch Parameters After reaching the desired level of strain, Using the Dial, hover over the “Frequency” press the Dial Button to confirm. parameter. This option allows you to set the frequency of stretch. Zoë-CM2 Culture Module / Use Page 28...
  • Page 29 Zoë-CM2 Programming Stretch Parameters Press the Dial Button to begin editing the frequency. After reaching the desired frequency, press the Dial Rotate the Dial to adjust the frequency between Button to confirm. 0.00 and 0.40 Hz. Zoë-CM2 Culture Module / Use Page 29...
  • Page 30 Zoë-CM2 Running Regulate™ Cycle The Regulate Cycle is designed to remove any microbubbles Using the Dial, hover over “Regulate Cycle” on the that may have been introduced during the activation and display. Press the Dial Button to select. seeding steps. Note: If modeling ALI or filling the top/bottom channel with gel, be sure to select “Air/Gel”...
  • Page 31 Zoë-CM2 A status bar and timer will appear and show progress of the Regulate Cycle. Running Regulate™ Cycle Rotate the Dial to bring up the “Start” option. Press the Dial Button to select “Start,” initiating the Regulate Cycle. To cancel the Regulate Cycle, rotate the Dial to bring up the “Cancel”...

  • Page 32: Shutting Down

    Zoë-CM2 Running Regulate™ Cycle Once the Regulate Cycle is complete, the status bar and timer will disappear, and the system will revert to the preset flow and stretch parameters. Shutting Down Press the Power Button on the front of Zoë. Zoë-CM2 Culture Module / Use Page 32...
  • Page 33 Settings Page 33...

  • Page 34: System Information

    Zoë-CM2 Settings System Information The “System Information” screen provides details on Zoë Model, Name (ID), Serial Number, Software Version, and Hardware ID. This screen can be accessed by hovering over To close the “System Information” screen, hover over and selecting the icon on the lower right of the screen.
  • Page 35 Updating Firmware & Downloading Logs Page 35...
  • Page 36 Zoë-CM2 Updating Firmware & Downloading Logs Updating Zoë Firmware To update the Zoë firmware, download the “Utility Hub” application from emulatebio.com/utility-hub. The application is compatible with computers running Windows 10 or a later version. Follow the instructions displayed on Utility Once Utility Hub has been downloaded, open the Hub to update the Zoë...

  • Page 37: Downloading Logs

    Zoë-CM2 Updating Firmware & Downloading Logs Downloading Logs Open Utility Hub and select “Download Logs”. Follow the instructions displayed on Utility Hub to download the logs from Zoë-CM2. Zoë-CM2 Culture Module / Updating Firmware & Downloading Logs Page 37...

  • Page 38: Help And Support

    Help & Support Contact Emulate Support for any issues with Zoë-CM2. Email: support@emulatebio.com Website: emulatebio.com/contact-support Phone: +1 844-902-4477 (Toll Free) +1 781-583-3515 Page 38...
  • Page 39 Care Page 39...

  • Page 40: Cleaning And Maintenance

    Care Cleaning & Maintenance Care for Zoë includes periodic cleaning. Preventative maintenance should be performed by a qualified Emulate Field Engineer at least once per year. Contact Emulate Support for additional details and to request an appointment. Regular checks and record keeping provide helpful information should any troubleshooting be required.

  • Page 41: Troubleshooting

    There is an unknown internal error with Zoë. Contact Emulate for troubleshooting. The Power Cord is damaged. Replace Power Cord; contact Emulate for replacement. Bay is empty or Tray is not fully inserted into the Bay. Insert Tray until the Tray snaps into place.
  • Page 42 User Guide EP-210 Rev G © Emulate, Inc., 2024. All rights reserved. For Terms and Conditions and Legal Notices refer to our website at: emulatebio.com/legal...

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