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AKTA design UPC-900 User Manual

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Monitor UPC-900
User Manual
18-1125-55

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  • Page 1 Monitor UPC-900 User Manual 18-1125-55...
  • Page 2 Trademarks Important user information ÄKTA, FPLC and UNICORN are trademarks of Amersham Pharmacia Biotech Limited or its subsidiaries. Meaning: Consult the instruction manual to avoid Amersham is a trademark of Nycomed Amersham plc. Pharmacia and Drop Design are trademarks of personal injury or damage to the product or other Pharmacia &...

  • Page 3: Table Of Contents

    Cleaning and checking the module ..............22 Cleaning the UV flow cell in-place...............23 Cleaning the UV flow cell off-line................23 Cleaning the conductivity flow cell in-place............24 Cleaning the conductivity flow cell off-line............24 Changing the conductivity flow cell..............24 Monitor UPC-900 User Manual 18-1125-55 Edition AC...
  • Page 4 Setup unit number ..............44 B.2.16 Setup display angle ..............44 Setting and using the alarm timer..............45 Service displays ..................45 Menu overview ..................46-47 Technical specifications ..................48 Operating data ..................48 Physical data .....................49 Accessories and Consumables ................51 Monitor UPC-900 User Manual 18-1125-55 Edition AC...
  • Page 5 About this manual This manual comprises two parts; a practical part (sections 1 – 5) and a reference part (sections A – D). Sections 1 – 5 contain the necessary information for operating the instrument. Monitor UPC-900 User Manual 18-1125-55 Edition AC...
  • Page 6 Contents Monitor UPC-900 User Manual 18-1125-55 Edition AC...

  • Page 7: Introduction

    Introduction 1 Introduction 1.1 General Monitor UPC-900 is a high precision on-line monitor for the combined measurement of UV absorption, pH and conductivity in liquid chromatography. The UPC-900 features: • Fixed wavelengths of 254, 280 nm (Hg lamp), or 214 nm (Zn lamp).

  • Page 8: Safety

    WARNING! HCl and NaOH are injurious to health. Avoid spillage. WARNING! Hg lamps contain small amounts of mercury and must be handled with care and disposed of according to national or local environmental regulations. Monitor UPC-900 User Manual 18-1125-55 Edition AC...

  • Page 9: Installation

    0.1 m is required behind and in front of the module. Do not place soft material under the module to ensure that the ventilation inlet below the front is not blocked. Monitor UPC-900 User Manual 18-1125-55 Edition AC...

  • Page 10: Installing The Optical Unit

    The optical unit can be placed up to 1.5 m from the monitor housing. Holder Optical unit Secure it by tightening the screw in the holder. Monitor UPC-900 User Manual 18-1125-55 Edition AC...
  • Page 11 The Hg optics with 254 and 280 nm filters and the Zn optics with the 214 nm filter are delivered with filters installed. If other filters are to be used, install the new filters as described in 2.10 Installing optical filters (optional). Monitor UPC-900 User Manual 18-1125-55 Edition AC...

  • Page 12: Installing The Conductivity Flow Cell

    Connect the conductivity cell to the socket Conductivity Flow Cell on the rear panel of the module. Connect the tubing with fingertight connectors. Monitor UPC-900 User Manual 18-1125-55 Edition AC...
  • Page 13 Note: If the electrode is not fully holder inserted, the system will leak and a dead volume will occur in the holder. Connect the pH electrode cable to the socket on the pH Probe rear of the module. Monitor UPC-900 User Manual 18-1125-55 Edition AC...

  • Page 14: Connecting Electrical Signal Cables

    As a stand-alone module, it is controlled from the front dial and display. In ÄKTA , the monitor is controlled from a PC running FPLC UNICORN ™ version 3.0 or higher via UniNet 1 cables, or manually from the front dial and display. Monitor UPC-900 User Manual 18-1125-55 Edition AC...

  • Page 15: Connecting To Supply Voltage

    PC and a termination plug. The UniNet 1 link connects, in series, the PC with Pump P-920, Monitor UPC-900 and the Frac-900. The termination plug is connected to the last module in the chain.

  • Page 16: Installing Optical Filters (Optional)

    10 Check that all filters are clean. Insert the filter wheel back into the filter housing. Note: The filter wheel can be placed only in correct position. 11 Reassemble the filter housing with the detector housing by fastening the four screws. Monitor UPC-900 User Manual 18-1125-55 Edition AC...

  • Page 17: Operation

    Return one menu level, or accept setting level Select sub-menu Return one menu level If a menu has sub levels, the sub menu is displayed by pressing Pressing moves back one menu level. Monitor UPC-900 User Manual 18-1125-55 Edition AC...
  • Page 18 The cursor moves back one place to the right every two seconds if the dial is not turned. The text or numerical value displayed is accepted by pressing . To cancel, press Monitor UPC-900 User Manual 18-1125-55 Edition AC...

  • Page 19: Main Menu Overview

    However, in most cases, a warm-up time of 15 minutes is sufficient. Switching the lamp on/off Switch the lamp on/off by moving the cursor with the dial, and Lamp then pressing OK. (on) Monitor UPC-900 User Manual 18-1125-55 Edition AC...

  • Page 20: Reading The Uv Absorbance Value

    Note: The user interface of the monitor must be unlocked if connected to a UNICORN control system. Setting the Cond analogue output Current analogue settings are displayed (zero and full scale Set Cond Analog. Out values). (00.00 - 50.00mS/cm) Monitor UPC-900 User Manual 18-1125-55 Edition AC...
  • Page 21 Allowed full range values are 0.0001, 0.0002, 0.0005, (0.005AUFS, 10%) 0.001, 0.002, 0.005, 0.01, 0-02, 0.05, 0.1, 0.2, 0.5, 1.0, 2.0 and 5.0. Zero level is set as a percentage of full scale. Monitor UPC-900 User Manual 18-1125-55 Edition AC...

  • Page 22: Calibrating Conductivity

    1 pH unit. Calibration can also be performed from UNICORN. In UNICORN, select . Select the pH System Control:System:Calibrate monitor. The calibration procedure can be done with the pH electrode either fitted in or removed from the flow cell. Monitor UPC-900 User Manual 18-1125-55 Edition AC...
  • Page 23 Turn the dial clockwise to access the buffer 2 calibrating menu. Calibr pH Buffer 2 Rinse the electrode tip with distilled water and then immerse the electrode in the second buffer solution (e.g. pH 4.0 or 9.0) and press Monitor UPC-900 User Manual 18-1125-55 Edition AC...
  • Page 24 Switch to the second standard buffer solution and repeat the procedure. For a description of calibration from UNICORN with the electrode fitted in the flow cell, see Chapter 6 in the UNICORN User Manual. Monitor UPC-900 User Manual 18-1125-55 Edition AC...

  • Page 25: Filtering Noise In The Uv Signal

    5 mm to 2 mm when a lower sensitivity is desired, due to output signal limitation. See section 2.3 Installing the optical unit, sub-section Flow cell installation. Monitor UPC-900 User Manual 18-1125-55 Edition AC...

  • Page 26: Reading Ph, Temperature And Conductivity Values

    Storage of the conductivity flow cell Overnight: The conductivity cell can be left filled with a buffer. Weekend or long term storage: Flush the conductivity cell with water and fill with 20% ethanol. Monitor UPC-900 User Manual 18-1125-55 Edition AC...

  • Page 27: Restart After Power Failure

    If the power supply to the module is interrupted, it automatically restarts when power is restored and displays the main operating menu. All set values are retained in the module and the lamp is switched on. Monitor UPC-900 User Manual 18-1125-55 Edition AC...

  • Page 28: Maintenance

    7000 hours (in coldroom, typically 2000h). • The lifetime of a Hg lamp at 280 nm, in room temperature is typically 3500 hours. • The lifetime of a Zn lamp is typically 2000 hours in room temperature. Monitor UPC-900 User Manual 18-1125-55 Edition AC...

  • Page 29: Cleaning The Uv Flow Cell In-Place

    2 After five squirts, leave the detergent solution in the flow cell for at least 20 minutes. 3 Pump the remaining detergent solution through the flow cell. 4 Rinse the syringe and then flush the flow cell with distilled water (10 ml). Monitor UPC-900 User Manual 18-1125-55 Edition AC...

  • Page 30: Cleaning The Conductivity Flow Cell In-Place

    See section B.2 in Reference information. If the cell constant is not known, it can be determined (see also section B.2 in Reference information). Monitor UPC-900 User Manual 18-1125-55 Edition AC...

  • Page 31: Cleaning The Ph Electrode

    Place the electrode tip in the heated KNO solution. Allow the electrode to cool while immersed in the KNO solution before re-testing. If these steps fail to improve the electrode response, replace the electrode. Monitor UPC-900 User Manual 18-1125-55 Edition AC...

  • Page 32: Changing The Ph Electrode

    Setup to select Press Setup UV Setup Lamp Run Time. Set the counter to zero with the dial. Press Lamp Run Time to acknowledge. Press three times to return to main menu 1. Monitor UPC-900 User Manual 18-1125-55 Edition AC...

  • Page 33: Trouble-Shooting

    2 Clean the system in accordance with the ÄKTA FPLC System Manual. 3 Clean the column in accordance with the column instructions. 4 Check that the mixer is functioning correctly and that the correct chamber volume is being used. Monitor UPC-900 User Manual 18-1125-55 Edition AC...
  • Page 34 8 Clean the pH electrode if required, see section 4.8 Cleaning the pH electrode. 9 Compare the response of the pH electrode with that of another pH electrode. If the response differs greatly, the electrode may require cleaning or replacement. Monitor UPC-900 User Manual 18-1125-55 Edition AC...
  • Page 35 1 Turn the flow cell so the end with the screws is facing the pH flow cell. 2 Recalibrate the conductivity cell. 3 Calibration solution, 1.00 M NaCl, not correctly prepared. Prepare a new calibration solution and recalibrate the conductivity cell. Monitor UPC-900 User Manual 18-1125-55 Edition AC...
  • Page 36 3.8 and section B.1.4 Setting the Cond analogue output Checking recorder No text on the front display 1 Check that the mains cable is connected and the power switch is in ON-position. Monitor UPC-900 User Manual 18-1125-55 Edition AC...

  • Page 37: Error Messages

    3 Check that optical unit is not exposed to direct sunlight. 4 If problem remains, call service Auto is not allowed from a level above 2 AU. 77 WARNING Autozero 2 Check buffers. Out of range 3 Clean UV cell. Monitor UPC-900 User Manual 18-1125-55 Edition AC...
  • Page 38 1 The difference between the zero level and full scale must be 97 WARNING pH scale at least 1 pH unit. Increase the span between zero and full (0-100%)<1pH unit scale settings. See section 3.8. Monitor UPC-900 User Manual 18-1125-55 Edition AC...
  • Page 39 ERROR number 118 1 Switch off the module. Exc x/y in ab.c 2 Check all connections. 3 Switch on the module. Exc DIV/O in ab.c Exc instr in ab.c Exc address in ab.c Monitor UPC-900 User Manual 18-1125-55 Edition AC...

  • Page 40: Reference Information

    Reference information Reference information Description A.1 Module Monitor UPC-900 is an on-line monitor for measurement of UV- absorption, pH (optional in ÄKTA ) and conductivity. The FPLC monitor can work with standard glass pH electrodes with a built in liquid-filled reference electrode and a BNC connector.

  • Page 41: Uv Optcal Unit

    For 214 nm wavelength, a Zn lamp is used. This lamp is larger than the Hg lamp and is therefore mounted in a larger lamp housing. The lamp connectors are keyed to inform the monitor software of which lamp type is connected. Monitor UPC-900 User Manual 18-1125-55 Edition AC...

  • Page 42: Ph Eletrode

    Monitor UPC-900 User Manual 18-1125-55 Edition AC...

  • Page 43: B Menu

    Service information relevant to the module can be checked. Information may not be available in all menus. Select main menu and press Check Select sub menu and press Check Service Mode Check Service Mode Monitor UPC-900 User Manual 18-1125-55 Edition AC...

  • Page 44: Setup Menus

    A warning message is shown until confirmed by pressing Warning! This will change temp calib The current adjustment value is displayed as default. Enter the Setup Adjust Temp temperature shown on the thermometer and press (22.3°C) 22.3 Monitor UPC-900 User Manual 18-1125-55 Edition AC...

  • Page 45: Setup Show Temperature

    Note: The conductivity temperature compensation must not be used when adjusting the cell constant. Set the Setup Cond Temp Comp 0 (see section B.2.4). The temperature sensor must be calibrated before adjusting the cell constant (see section B.2.2). Monitor UPC-900 User Manual 18-1125-55 Edition AC...
  • Page 46 (83.53mS/cm) 86.6 displayed as default. Enter the theoretical conductivity value according to the graph and press The new cell constant is automatically calculated. The range is 1.000- 999.9 mS/cm. Temperature (°C) Monitor UPC-900 User Manual 18-1125-55 Edition AC...

  • Page 47: Setup Adjust Cell Constant

    (on) current conductivity is displayed in main menus 1 and 2. If shown, no conductivity is displayed in the main menus. Change the setting if desired and press Setup Show Cond (on) Monitor UPC-900 User Manual 18-1125-55 Edition AC...

  • Page 48: Setup Ph Temperature Compensation

    Set Averaging filter constant is shown. Valid values are 0.02, 0.04, 0.08, 0.16, (1.3S) 0.32, 0.64, 1.3, 2.6, 5 and 10 seconds. Change the setting if desired and press Set Averaging (1.3S) 0.64 Monitor UPC-900 User Manual 18-1125-55 Edition AC...

  • Page 49: Setup Lamp Run Time

    Select sub menu and press Setup Language Setup Language (GB) GB D E F I Select the desired language. GB = English D = German F = French E = Spanish = Italian Monitor UPC-900 User Manual 18-1125-55 Edition AC...

  • Page 50: Setup Unit Number

    UniNet-bus. It should correspond to the number set in UNICORN for the Monitor UPC-900. The number should be set to 0 if one UPC-900 is used. If more than one UPC-900 monitor is used, they must all have different numbers.

  • Page 51: Setting And Using The Alarm Timer

    B.4 Service displays The module has service displays for use by authorised service Insert Access Code: personnel. If the service display is accidentally Insert Access Code: selected, press to return to the normal operation display. Monitor UPC-900 User Manual 18-1125-55 Edition AC...

  • Page 52: Menu Overview

    Setup Setup Temp Cond Setup Language (GB) Set Clock Setup Unit Number Set Timer Setup Display Angle (18:34:52) 00.00.00 ( ->|) ->\ ->| ->/ Set Alarm 00:32:21 Alarm/Timer 12:30:52 Alarm/Timer off? Buzzer 08:23:34 Monitor UPC-900 User Manual 18-1125-55 Edition AC...
  • Page 53 Set Averaging UV sub menus (1.3S) 0.64 Set Averaging (1.3S) Setup Lamp Run Time Setup Lamp Run Time Hg (2000h) Hg (2000h) 3000 Setup Show UV (on) Setup Show UV (on) on off Monitor UPC-900 User Manual 18-1125-55 Edition AC...

  • Page 54: C Technical Specifications

    Max. ±0.5% of full scale calibrated range Response time <3 s (0 - 95% of step) Temperature sensor accuracy ±2.0 °C drift ±0.5 °C per 10 h Flow rate sensitivity ±1% within 0–100 ml/min Monitor UPC-900 User Manual 18-1125-55 Edition AC...

  • Page 55: Physical Data

    Wetted materials UV flow cells Quartz, ETFE, titanium cond. flow cell Titanium, CTFE pH electrode Glass, FFKM (perfluororubber) flow cell titanium dummy electrode PTFE (polytetrafluoroethylene) pH stability range 1–13 (1–14, <1 day exposure) Monitor UPC-900 User Manual 18-1125-55 Edition AC...
  • Page 56 CE labelled Amersham Pharmacia Biotech modules or other products as recommended. S afety standards This product meets the requirement of the Low Voltage Directive (LVD) 73/23/EEC through the harmonised standard EN 61010-1. Monitor UPC-900 User Manual 18-1125-55 Edition AC...

  • Page 57: D Accessories And Consumables

    Dummy electrode, round tip 18-1111-03 pH electrode complete, round tip, incl. 18-1134-84 pH electrode, flow cell and holder Flow cell holder UPC-900 18-3055-87 Clamp, Conductivity flow cell 18-1111-14 PEEK tubing, i.d. 0.50 mm, o.d. 1/16” (G) 2 m 18-1113-68 PEEK tubing, i.d.
  • Page 60 Short instructions The following short instructions are intended as a guide to users who are fully familiar with the safety precautions and operating instructions described in this manual. These short instructions assume that the module is installed according to the installation instructions.

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