Table of Contents
Advertisement
Advertisement
Table of Contents
Related Manuals for AKTA design UV-900
Summary of Contents for AKTA design UV-900
- Page 1 Monitor UV-900 User Manual 18-1120-05...
- Page 2 Important user information Trademarks ÄKTA and UNICORN are trademarks of Amersham Pharmacia Biotech Limited or its subsidiaries. Meaning: Consult the instruction manual to avoid Amersham is a trademark of Nycomed Amersham plc. personal injury or damage to the product or other Pharmacia and Drop Design are trademarks of equipment.
-
Page 3: Table Of Contents
3.12 Restart after power failure ...................12 Maintenance Periodic maintenance ...................13 Cleaning-in-place ....................13 Checking the instrument ..................13 Cleaning the flow cell and optical fibre connectors..........14 Instrument housing ....................14 Trouble shooting General ........................15 Faults and actions ....................15 Error messages .....................16 Monitor UV-900 User Manual 18-1120-05 Edition AB... - Page 4 About this manual This manual comprises two parts; a practical part (sections 1 – 5) and a reference part (sections A – D). Sections 1 – 5 contain the necessary information for operating the instrument. Monitor UV-900 User Manual 18-1120-05 Edition AB...
-
Page 5: Introduction
Introduction 1 Introduction 1.1 General Monitor UV-900 is a UV-absorption monitor for use in liquid chromatography. Monitor UV-900 features: • Variable wavelength for detection in the range 190–700 nm. • Up to 3 wavelengths can be monitored simultaneously. • Two alternative flow cells with path length 2 mm and 10 mm. -
Page 6: Installation
Handle the optical fibres with care, do not bend excessively. Two flow cells are available: UV-900/2 with 2 mm light path length (cell volume 2 µl) and UV-900/10 with 10 mm path length (8 µl). Monitor UV-900 User Manual 18-1120-05 Edition AB... - Page 7 To improve the access to the tubing connections, the flow cell can alternatively be positioned with the tubing connections horizontal. Normal position Alternative position Serial Serial Number Number Monitor UV-900 User Manual 18-1120-05 Edition AB...
- Page 8 Make sure that the sleeves are pushed tight to the housing to prevent dust or fluid entering the connection. 5. To “run-in” the new optical fibres prior to its use in normal operation, run the monitor for 8 hours at 215 nm. Monitor UV-900 User Manual 18-1120-05 Edition AB...
-
Page 9: Connecting Electrical Signal Cables
PC and the termination plug. The UniNet 1 link connects, in series, the PC with Pump P-900, Monitor pH/C-900, Monitor UV-900 and Frac-900. The termination plug is connected to the last instrument in the chain. Monitor UV-900... -
Page 10: Connecting To Supply Voltage
The cover is a simple push fit onto the cell holder. Two small lugs on the cover locate in holes at the front and rear of the cell holder The cover is then lowered over the cell holder. Monitor UV-900 User Manual 18-1120-05 Edition AB... -
Page 11: Operation
Main menu 1 Main menu 2 Sub menu 3.1 Sub menu 3.1.1 Main menu 3 Main menu 4 Sub menu 3.2 Sub menu 3.3.1 Sub menu 3.3 Main menu 5 Sub menu 3.3.2 Monitor UV-900 User Manual 18-1120-05 Edition AB... -
Page 12: Main Menu Overview
(2.56s) Check internal operating values. Check See Reference information section B.1. Setup language, unit number etc. Setup See Reference information section B.2. Set different timer options. Alarm/Timer 00:22:24 See Reference information section B.3. Monitor UV-900 User Manual 18-1120-05 Edition AB... -
Page 13: Setting Lamp On/Off
Set Wavelength λ λ 3 5 Repeat step 4 in the menu 6 Press ESC to return to the main operating menu. In UNICORN, the wavelengths are set with the instruction Wavelength System Control:Manual:Alarm&Mon Monitor UV-900 User Manual 18-1120-05 Edition AB... -
Page 14: Autozero
3 Select menu , press OK. Set Zero Level 4 Set the value, press OK. This value determines where the zero absorbance level should be in relation to full scale on the recorder. Monitor UV-900 User Manual 18-1120-05 Edition AB... -
Page 15: Filtering Noise
Time constant (s) Min. peak width (approximate) at half height (s) 5.12 2.56 1.28 0.64 0.32 0.16 0.05 0.08 0.03 In UNICORN the averaging time is set with the instruction AveragingTime System Control:Manual:Alarm&Mon Monitor UV-900 User Manual 18-1120-05 Edition AB... -
Page 16: Changing Flow Cell
If the power supply to the instrument is interrupted, the instrument automatically restarts itself and displays the main operating window. All set values are retained in the instrument but the instrument starts with the lamp switched-off. Monitor UV-900 User Manual 18-1120-05 Edition AB... -
Page 17: Maintenance
Check Lamp Run Time 1 Select menu , press OK. Check 200h 2 Select menu Check Lamp Run Time If the lamp ontime is >4000 hours, contact Amersham Pharmacia Biotech for lamp replacement. Monitor UV-900 User Manual 18-1120-05 Edition AB... -
Page 18: Cleaning The Flow Cell And Optical Fibre Connectors
(10 ml). Cleaning the optical fibre connectors Wipe with 30% isopropanol on lens paper. 4.5 Instrument housing Wipe the instrument housing regularly with a damp cloth. Let the instrument dry completely before use. Monitor UV-900 User Manual 18-1120-05 Edition AB... -
Page 19: Trouble Shooting
Trouble shooting 5 Trouble shooting 5.1 General When contacting Amersham Pharmacia Biotech for support, state Monitor UV-900 the program version of the instrument, which is shown for 2 seconds V1.00 after the self-test, during switch-on, or in the menu Check Service , see section B.1.6. -
Page 20: Error Messages
ERROR key(OK) ERROR key (Esc) ERROR key (OK+Esc). ERROR 100 ERROR 109-113 ERROR 120-121 1 Switch off the instrument. ERROR 106-108 2 Check all UniNet1 connections. ERROR 118 3 Switch on the instrument. Monitor UV-900 User Manual 18-1120-05 Edition AB... -
Page 21: Reference Information
Reference information Reference information Description A.1 Instrument The Monitor UV-900 is a UV-absorption monitor with variable wavelength. The instrument contains no internal user replaceable items. Analogue out Remote UniNet 1 Mains On/off 0-1V Connector/switch Function Analogue out 0–1V Recorder output, 3 channels 0–1 V... -
Page 22: Flow Cell
50% of the light passing through the sample fibre (S) and the flow cell, and 50% being directed through the reference fibre (R). Two photodiodes with identical characteristics monitor the intensities of the measuring and reference beams. Monitor UV-900 User Manual 18-1120-05 Edition AB... - Page 23 At calibration the instrument automatically finds 2 persistent lines in the spectrum of Xenon. The wavelengths of these known lines are used to calibrate the stepper motor that turns the grating. Monitor UV-900 User Manual 18-1120-05 Edition AB...
-
Page 24: Menus
1 Select main menu , press OK. Check 2 Select sub menu press OK. The recalibration starts and Recalibrate, after approx. 60 seconds the message Recalibration finished Recalibrating shown. Please wait... Recalibration finished Monitor UV-900 User Manual 18-1120-05 Edition AB... -
Page 25: Check Analogue Out
012345678901 Serial Number 5 The instrument serial number is displayed, press OK. 01234567 YM 012345 Monitor UV-900 6 The instrument name and software version are displayed, press V1.00 Date of Maintenance 7 The date of the last service is displayed, press OK. -
Page 26: Setup Unit Number
00:00:00, an alert display is shown and the instrument beeps, Bzz00:00:29 13:08:45 !! Alarm time !! until the OK button is pressed. The display shows the time elapsed since the alarm, and the current time. Monitor UV-900 User Manual 18-1120-05 Edition AB... -
Page 27: Service Displays
The instrument has service displays for use by authorised service personnel. If the service display is accidentally Insert Access Code: Insert Access Code: selected, press the ESC-button to exit to the normal operation display. Monitor UV-900 User Manual 18-1120-05 Edition AB... -
Page 28: Menu Overview
(GB) GB D F E I Setup Unit number Setup Unit number Set Display Angle ( ->|) ->\ ->| ->/ Alarm/Timer 00:22:24 Alarm/Timer action Set Alarm 00:32:21 Set Timer (18:34:52) 00.00.00 Set Clock Alarm/Timer off? Monitor UV-900 User Manual 18-1120-05 Edition AB... -
Page 29: C Technical Specifications
2 µl 10 mm cell 8 µl Degree of protection IP 21 Wetted materials PTFE (polytetrafluoroethylene) PEEK (polyetheretherketone) titanium (palladium alloy) Quartz (synthetic fused silica) pH stability range 1–13, 1–14 (<1 days exposure) Monitor UV-900 User Manual 18-1120-05 Edition AB... - Page 30 CE labelled Amersham Pharmacia Biotech instruments or other products as recommended. Safety Standards This product meets the requirement of the Low Voltage Directive (LVD) 73/23/EEC through the harmonized standard EN 61010-1. Monitor UV-900 User Manual 18-1120-05 Edition AB...
-
Page 31: D Accessories And Spare Parts
Reference information Accessories and spare parts Item Quantity per pack Code no. Monitor UV-900 complete but without flow cells 18-1108-35 Flow cell UV-900/2 (2 mm) 18-1111-10 Flow cell UV-900/10 (10 mm) 18-1111-11 Signal cable 18-1110-64 Fibre detachment tool 18-1111-16 Teflon tubing, i.d. 1/8", o.d. 3/16"... -
Page 36: Short Instructions On
Short instructions The following short instructions are intended as a guide to users who are fully familiar with safety precautions and operating instructions described in this manual. The instructions assumes that the instrument is installed according to the installation instructions. Select menu/value by means of the mains...
Need help?
Do you have a question about the UV-900 and is the answer not in the manual?
Questions and answers