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Related Manuals for THORLABS Cerna SFM
Summary of Contents for THORLABS Cerna SFM
- Page 1 Cerna® Mini Microscopes User Guide...
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Page 3: Table Of Contents
Fluorescence Filter Specifications (SFMGFP2) ....20 Objective Specifications (SFMGFP2) ......... 20 6.10 sCMOS Camera Specifications (SFMGFP2) ...... 21 Chapter 7 Mechanical Drawings ..............24 Chapter 8 Warranty ..................33 Chapter 9 Regulatory ..................34 Chapter 10 Thorlabs Worldwide Contacts ............. 35... -
Page 4: Chapter 1 Warning Symbol Definitions
Cerna Mini Microscopes Chapter 1: Warning Symbol Definitions Chapter 1 Warning Symbol Definitions Below is a list of warning symbols you may encounter in this manual or on your device. Symbol Description Direct Current Alternating Current Both Direct and Alternating Current Earth Ground Terminal Protective Conductor Terminal Frame or Chassis Terminal... -
Page 5: Chapter 2 Safety
WARNING DO NOT use the device for anything other than its intended use. If the device is used in a manner not specified by Thorlabs, the protection provided by the equipment may be impaired. CAUTION DO NOT operate in a wet, damp, or explosive environment. -
Page 6: Chapter 3 Description
Description The Cerna Mini Microscopes provide basic microscope configurations that are ideal for experiments requiring simple imaging. Thorlabs offers three microscope configurations: SFM2 (Cerna Mini Microscope), SFM (Cerna Mini Microscope with Epi-Illuminator Module), and SFMGFP2 (Cerna Mini Microscope with Epi- Illuminator Module for GFP/Alexa Fluor®... -
Page 7: Overview
Cerna Mini Microscopes Chapter 3: Description Blue LED for GFP Excitation ▪ 470 nm Nominal Wavelength ▪ 1161.7 mW Typical Output Power at Max Current ▪ 28 nm FWHM (Full Width at Half Max) Bandwidth Overview Camera Port Filter Cube Housing MCM301 MCMK3 Focus... - Page 8 Cerna Mini Microscopes Chapter 3: Description Camera Port MCM301 MCMK3 Focus Figure 2 SFM2 Microscope Module Rev. H, January 31, 2024 Page 5...
- Page 9 Cerna Mini Microscopes Chapter 3: Description sCMOS Camera Camera Port Filter Cube Housing N10X-PF SFM80 MCMK3 MCM301 Focus T-Cube Figure 3 SFMGFP2 Microscope Module LED Driver Page 6 TTN152587-D02...
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Page 10: Chapter 4 Getting Started
Cerna Mini Microscopes Chapter 4: Getting Started Chapter 4 Getting Started This section is provided for those interested in getting the Cerna Mini Microscopes up and running quickly. Ordering Codes Ordering Codes Description Cerna Mini Microscope with Epi-Illuminator Module SFM2 Cerna Mini Microscope Cerna Mini Microscope with Epi-Illuminator Module for SFMGFP2... -
Page 11: Cerna Mini Microscope Setup
Guide ThorCam Camera Software CD Inspect the device and its accessories for any missing parts or damage. If there is any problem, please contact our nearest office (see Thorlabs Worldwide Contacts on page 31 for details). Cerna Mini Microscope Setup Note: To set up the SFM and SFM2 microscopes, follow the procedure in Section 4.3.1. -
Page 12: Microscope
Cerna Mini Microscopes Chapter 4: Getting Started Requirements: The following additional accessories (not included) are required for installation of the microscope: • 1.5 mm Hex Key • 2 mm Hex Key • 2.5 mm Hex Key • 5/64" Hex Key 4.3.1 Microscope Use four SHCS (Socket Head Cap Screw) (M6 for metric, 1/4"-20 x 1/2"... - Page 13 Cerna Mini Microscopes Chapter 4: Getting Started M4 x 12 mm Screw Figure 5 Mounting the Filter Cube Housing For SFM, use the 2 mm hex key to mount the Camera Port onto the Filter Cube Housing. For SFM2, mount the Camera Port directly to the Dovetail Plate.
- Page 14 Cerna Mini Microscopes Chapter 4: Getting Started Connect the adapter cables from the Focus Module and stages to the 3- Axis Controller Box. Connect the cable from the 3-Axis Knob Box to the 3-Axis Controller Box. Connect the power supply from the 3-Axis Controller Box to a standard power outlet.
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Page 15: Fluorescence Filter Installation
Install your filter set inside the filter cube. See the MDFM-MF2 manual for detailed instructions- https://www.thorlabs.com/manuals.cfm Slide the filter cube on the Filter Cube Housing cover and tighten the screw at the center of the cover... -
Page 16: Led Power Source Installation
Cerna Mini Microscopes Chapter 4: Getting Started 4.3.3 LED Power Source Installation The set-up uses a 15 V, 2.4 A Power Supply Unit with 3.5 mm Jack Connector. For easy installation, the Filter Cube Housing included in the SFMGFP2 configuration comes with a pre-installed LED and SFM80 collimation tube. Use two SHCS to mount the T-Cube LED Driver onto the optical table/breadboard. -
Page 17: Camera
Cerna Mini Microscopes Chapter 4: Getting Started 4.3.4 Camera Mount the sCMOS camera on the Camera Port. Refer to the included camera user guide for setup and installation. Figure 8 Mounting the Camera Page 14 TTN152587-D02... -
Page 18: Chapter 5 Maintaining The Sfm Series
Maintaining the SFM Series There are no serviceable parts in the SFM series modules. If you suspect a problem with your module, please contact our nearest office (see Thorlabs Worldwide Contacts on page 31 for details) for assistance from an applications engineer. -
Page 19: Chapter 6 Specifications
Cerna Mini Microscopes Chapter 6: Specifications Chapter 6 Specifications Microscope Body Specifications Specification Value Rail Length 174.3 mm (6.86") Post Length 200 mm (7.87") 162 mm (6.38”) Post Manual Height Adjustment Throat Depth 152.4 mm (6") Fine Motorized Motion X,Y, and Z Axes 1″... -
Page 20: Motion Controller
Cerna Mini Microscopes Chapter 6: Specifications Stepper Motor Specifications Thread Screw Pitch 0.3 mm Step Angle 1.8° Limit Switches Hall Effect Sensors Phase Current 0.49 A 5.1 Ω Phase Resistance 20 MΩ Insulation Resistance Motion Controller Specification Value Item # MCM301 Motor Output Motor Drive Voltage... -
Page 21: Led Collimation Module Compatibility (Sfm/Sfmgfp2)
Cerna Mini Microscopes Chapter 6: Specifications LED Collimation Module Compatibility (SFM/SFMGFP2) Collimation Module Item # Compatible LEDs For Mounted LEDs with 80 - 120° Viewing SFM80 (Included with SFMGFP2) Angle, 350 - 700 nm Wavelength For Mounted LEDs with <22° Viewing Angle, SFM22 400 - 700 nm Wavelength LED Specifications (SFMGFP2) -
Page 22: T-Cube Led Driver Specifications
Cerna Mini Microscopes Chapter 6: Specifications T-Cube LED Driver Specifications Specification Value Common Data Item # LEDD1B Output Current Range 0 - 1200 mA LED Current Limit Set Point Range 200 - 1200 mA 11 V (Min) LED Forward Voltage 12 V (Typical) Current Ripple 8 mA... -
Page 23: Fluorescence Filter Specifications (Sfmgfp2)
Cerna Mini Microscopes Chapter 6: Specifications Fluorescence Filter Specifications (SFMGFP2) Value Specification Excitation Emission Dichroic Filter Filter Filter Item # MDF-GFP Size Ø25 ± 0.1 mm Ø25 ± 0.1 mm 25.0 mm x 36.0 mm Clear Aperture >Ø21 mm >Ø21 mm >(22.5 mm x 32.4 mm) Angle of Incidence 0°... -
Page 24: Scmos Camera Specifications (Sfmgfp2)
Cerna Mini Microscopes Chapter 6: Specifications Figure 9 Objective Dimensional Drawing 6.10 sCMOS Camera Specifications (SFMGFP2) Specification Value Item # CM2100M-USB Sensor Type Monochrome sCMOS Effective Number of Pixels (Horizontal x 1920 x 1080 Vertical) Imaging Area (Horizontal x Vertical) 9.6768 mm x 5.4432 mm Pixel Size 5.04 µm x 5.04 µm... - Page 25 Cerna Mini Microscopes Chapter 6: Specifications Digital Output 16 Bit Dynamic Range Up to 87 dB ≥23 ke Full Well Lens Mount C-Mount (1.000"-32) 3.7 W @ 30 fps (Full Sensor ROI) USB Power Consumption 4.3 W @ 50 fps (Full Sensor ROI) Recommended System Requirements ...
- Page 26 Cerna Mini Microscopes Chapter 6: Specifications Figure 10 Quantum Efficiency of CS2100M-USB Camera Rev. H, January 31, 2024 Page 23...
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Page 27: Chapter 7 Mechanical Drawings
Cerna Mini Microscopes Chapter 7: Mechanical Drawings Chapter 7 Mechanical Drawings Figure 11 Mechanical Drawing of SFM Module (Side View) Page 24 TTN152587-D02... - Page 28 Cerna Mini Microscopes Chapter 7: Mechanical Drawings Figure 12 Mechanical Drawing of SFM Module (Front and Back View) Rev. H, January 31, 2024 Page 25...
- Page 29 Cerna Mini Microscopes Chapter 7: Mechanical Drawings Figure 13 Mechanical Drawing of SFM Module (Top and Bottom View) Page 26 TTN152587-D02...
- Page 30 Cerna Mini Microscopes Chapter 7: Mechanical Drawings Figure 14 Mechanical Drawing of SFM2 Module (Side View) Rev. H, January 31, 2024 Page 27...
- Page 31 Cerna Mini Microscopes Chapter 7: Mechanical Drawings Figure 15 Mechanical Drawing of SFM2 Module (Front and Back View) Page 28 TTN152587-D02...
- Page 32 Cerna Mini Microscopes Chapter 7: Mechanical Drawings Figure 16 Mechanical Drawing of SFM2 Module (Top and Bottom View) Rev. H, January 31, 2024 Page 29...
- Page 33 Cerna Mini Microscopes Chapter 7: Mechanical Drawings Figure 17 Mechanical Drawing of SFMGFP2 Module (Side View) Page 30 TTN152587-D02...
- Page 34 Cerna Mini Microscopes Chapter 7: Mechanical Drawings Figure 18 Mechanical Drawing of SFMGFP2 Module (Front and Back View) Rev. H, January 31, 2024 Page 31...
- Page 35 Cerna Mini Microscopes Chapter 7: Mechanical Drawings Figure 19 Mechanical Drawing of SFMGFP2 Module (Top and Bottom View) Page 32 TTN152587-D02...
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Page 36: Chapter 8 Warranty
1 year starting with the date of shipment in accordance with and subject to the terms and conditions set forth in Thorlabs’ General Terms and Conditions of Sale. Incorporated light sources are warrantied for the lesser of one year or (to the extent applicable) the number of hours stated in the specifications. -
Page 37: Chapter 9 Regulatory
Waste Treatment is Your Own Responsibility If you do not return an “end of life” unit to Thorlabs, you must hand it to a company specialized in waste recovery. Do not dispose of the unit in a litter bin or at a public waste disposal site. -
Page 38: Chapter 10 Thorlabs Worldwide Contacts
Cerna Mini Microscopes Chapter 10: Thorlabs Worldwide Contacts Chapter 10 Thorlabs Worldwide Contacts USA, Canada, and South America UK and Ireland Thorlabs, Inc. Thorlabs Ltd. sales@thorlabs.com sales.uk@thorlabs.com techsupport@thorlabs.com techsupport.uk@thorlabs.com Europe Scandinavia Thorlabs GmbH Thorlabs Sweden AB europe@thorlabs.com scandinavia@thorlabs.com France Brazil Thorlabs SAS Thorlabs Vendas de Fotônicos Ltda. - Page 39 www.thorlabs.com...
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