THORLABS CM201 User Manual
  1. Manuals
  2. Brands
  3. THORLABS Manuals
  4. Microscope
  5. CM201
  6. User manual
THORLABS CM201 User Manual

THORLABS CM201 User Manual

Green fluorescent protein confocal microscope
Hide thumbs
Table of Contents

Advertisement

Quick Links

Download this manual
CM201
Green Fluorescent Protein
Confocal Microscope
User Guide

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the CM201 and is the answer not in the manual?

Questions and answers

Related Manuals for THORLABS CM201

Summary of Contents for THORLABS CM201

  • Page 1 CM201 Green Fluorescent Protein Confocal Microscope User Guide...
  • Page 2 Copyright January 28, 2022, Thorlabs, Inc. All rights reserved. Information in this document is subject to change without notice. The software described in this document is furnished under a license agreement or nondisclosure agreement. The software may be used or copied only in accordance with the terms of those agreements.

  • Page 3: Table Of Contents

    3.2. Imaging Capabilities ..................... 3 Chapter 4 Getting Started ........................4 4.1. Unpacking and Inspection ................... 4 4.2. Setting up the CM201 ....................5 4.3. Cable Connection Diagram ..................8 4.3.1. Electronic Control Unit (ECU) Connections ................9 4.3.2. Galvo-Galvo Scanning Pair ....................10 Alignment ..........................

  • Page 5: Chapter 1 Warning Symbol Definitions

    CM201 Chapter 1: Warning Symbol Definitions Warning Symbol Definitions Chapter 1 Below is a list of warning symbols you may encounter in this manual or on your device. Symbol Description Direct Current Alternating Current Both Direct and Alternating Current Earth Ground Terminal...

  • Page 6: Chapter 2 Safety

    Any modification or servicing of this system by unqualified personnel renders Thorlabs free of any liability. Only personnel authorized by Thorlabs and trained in the maintenance of this equipment should remove any covers or housings or attempt any repairs or adjustments.

  • Page 7: Chapter 3 Description

    Z-axis control. The CM201 features a movable silver-coated mirror on a manual slider at the front of the scan path that allows users to select between confocal and widefield imaging modalities without replacing the optic. The confocal microscope is designed so that imaging capabilities can be added to accommodate new experimental needs as your research requirements grow.

  • Page 8: Chapter 4 Getting Started

    This section is provided for those interested in getting the GFP Confocal Microscope up and running quickly. 4.1. Unpacking and Inspection Open the package, and carefully remove the CM201 and its accessories. Unpack the laser, Galvo-Galvo Controller, PMT, computer, and monitor from their respective packages. The table lists the standard accessories shipped with the device.

  • Page 9: Setting Up The Cm201

    DO NOT place the SMA to BNC cable or DB-25 Connector cable near any electrical noise sources such as a power cable. This may cause poor quality images. A. Microscope 1. Use four screws (M6 for metric/1/4-20x5/8" for imperial) to mount the CM201 onto the optical table. See the Mechanical Drawing Chapter on Page 26 for mounting holes.
  • Page 10 Make sure the laser source is off. 1. Remove the red cap from the CM201 back panel. 2. Connect one end of the SM (Single Mode) Fiber Patch cable to the laser and the other end to the CM201 back panel.
  • Page 11 3. Connect the SMA to BNC cable from the OUT port of the PMT to the AI1 port on the NI Breakout Box. 4. Connect one end of the Armored Fiber Patch cable to the PMT and the other end to the Pinhole port of the CM201. Rev G, January 28, 2022...

  • Page 12: Cable Connection Diagram

    CM201 Chapter 4: Getting Started 4.3. Cable Connection Diagram Page 8 TTN118795-D02...

  • Page 13: Electronic Control Unit (Ecu) Connections

    Electronic Control Unit (ECU), Rear View l X IN: This is the command signal for the X galvanometer. It is not used in Thorlabs complete imaging systems setups. It may be monitored without interrupting the system, so long as the impedance is 1 kΩ.

  • Page 14: Galvo-Galvo Scanning Pair

    CM201 Chapter 4: Getting Started 4.3.2. Galvo-Galvo Scanning Pair With Galvo-Galvo systems there are two galvos, a fast (X axis) galvo, and a slow (Y axis) galvo. The fast Galvo moves across the image linearly, with even velocity. It takes 200 µs to turnaround at the edges of the image.
  • Page 15 CM201 Chapter 4: Getting Started The slow galvo (Y axis) moves once per image frame. During each sweep of the Y galvo scanner the ECU is signaling a frame trigger (FRAME TRIGGER OUT or vertical line sync) for acquisition synchronization.

  • Page 16: Chapter 5 Alignment

    CM201 Chapter 5: Alignment Alignment Chapter 5 You must align the microscope before imaging. There are two Alignment procedures: Fluorescence Slide Alignment and Bead Slide Alignment. A. Fluorescence Slide Alignment Perform the Fluorescence Slide Alignment procedure prior to Bead Slide Alignment.
  • Page 17 CM201 Chapter 5: Alignment 3. Use the slider on the objective holder to move it to its rear position and then attach the Alignment tool to the front port of the objective arm. 4. Turn on all devices. For the laser, turn the POWER key switch clockwise. The unit is ON when the display lights up. DO NOT activate the laser at this step.
  • Page 18 CM201 Chapter 5: Alignment 7. In the Galvo/Galvo Area Control panel, select Advanced and click Page 14 TTN118795-D02...
  • Page 19 Use the iris on the Alignment tool to resize the beam on the center of the IR Disk. If the beam on the IR Disk appears clipped (not circular), please contact Thorlabs for assistance. 10. Press and release the ENABLE switch to turn off the laser.
  • Page 20 CM201 Chapter 5: Alignment 12. Mount the objective onto the microscope. 13. Use four screws and washers to mount the Rigid Stand Slide Holder on the optical table. Place the fluorescence slide on the holder. 14. Press and release the ENABLE switch to activate the laser.
  • Page 21 CM201 Chapter 5: Alignment 15. In the ThorImageLS application, set the parameters in the Galvo/Galvo Scanner Control panel as shown in the image below: 16. In the Galvo/Galvo Area Control panel, set the parameters as shown in the image below: 17.
  • Page 22 CM201 Chapter 5: Alignment 18. Use the 2 mm hex key to adjust the XY Mount inside the microscope (the SM Patch cable is connected to the XY Mount) in the X and Y directions to center the maximum beam intensity at the center of the image.
  • Page 23 CM201 Chapter 5: Alignment B. Bead Slide Alignment Make sure to perform the Fluorescence Slide Alignment procedure prior to Bead Slide Alignment. 1. Remove the fluorescence slide and place the bead slide on the Rigid Stand Slide Holder. 2. In the ThorImageLS application, set the parameters in the Galvo/Galvo Scanner Control panel as shown in the image below: 3.
  • Page 24 CM201 Chapter 5: Alignment 5. In the Z Control panel, set the Slider Step Size to 1 µm. Click Set next to the Start field to store the Start location. 6. In Z Control panel, use the ± button to gradually move the objective up (20 µm distance from the Start position).
  • Page 25 CM201 Chapter 5: Alignment ▪ If there is only one maximum intensity for the image, move the objective to the maximum intensity point and adjust the pinhole’s X and Y position to maximize the intensity. One Maximum Intensity Rev G, January 28, 2022...
  • Page 26 CM201 Chapter 5: Alignment ▪ If there are two maximum intensities for the image, move the objective to the mid-point of the two maximum intensities. Then adjust the pinhole’s X and Y position to maximize the intensity. After the adjustment, you can see only one maximum intensity for the image.

  • Page 27: Chapter 6 Maintaining The Cm201

    Maintaining the CM201 Chapter 6 The unit does not need regular maintenance. If you suspect a problem with your CM201, please contact our nearest office for assistance from an application engineer (see Thorlabs Worldwide Contacts Chapter on Page 28 for details).

  • Page 28: Chapter 7 Specifications

    CM201 Chapter 7: Specifications Specifications Chapter 7 Specification Value Excitation Laser Single Mode Fiber-Coupled Laser Wavelength 488 nm Max Output Power 16.0 mW (Min) Power Control Manual, 0 to 5 V External Modulation Signal Scanning Scan Head Galvo/Galvo Protected-Silver Coated with λ/4 Surface Flatness (P-V)
  • Page 29 CM201 Chapter 7: Specifications Specification Value General Microscope Features Silver-Coated Mirror on a Manual Slider to Switch Between Confocal and Widefield Imaging Widefield Viewing D1N Dovetail on Top of Scan Path to Mount Cerna Widefield Viewing Accessories 95 mm Dovetail Rail to Mount Cerna Body Attachments,...

  • Page 30: Chapter 8 Mechanical Drawing

    CM201 Chapter 8: Mechanical Drawing Mechanical Drawing Chapter 8 Page 26 TTN118795-D02...

  • Page 31: Chapter 9 Regulatory

    Waste Treatment is Your Own Responsibility If you do not return an “end of life” unit to Thorlabs, you must hand it to a company specialized in waste recovery. Do not dispose of the unit in a litter bin or at a public waste disposal site.

  • Page 32: Chapter 10 Thorlabs Worldwide Contacts

    CM201 Chapter 10: Thorlabs Worldwide Contacts Chapter 10 Thorlabs Worldwide Contacts For technical support or sales inquiries, please visit us at www.thorlabs.com/contact for our most up-to-date contact information. USA, Canada, and South America UK and Ireland Thorlabs, Inc. Thorlabs Ltd.
  • Page 33 www.thorlabs.com...

Table of Contents