Related Manuals for PicoQuant Single Channel SPAD Detection Unit
Summary of Contents for PicoQuant Single Channel SPAD Detection Unit
- Page 1 Single Channel SPAD Detection Unit External Detection Unit for Laser Scanning Microscopes User's Hardware Manual and Technical Data Version 3.1...
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Page 2: Table Of Contents
„Sepia“ diode laser operation manual. If your instrument uses another excitation system, follow the safety instructions of the relevant manual. The delivered instruments are pre-set by PicoQuant to operate on the power outlet line voltage for the country of delivery. Nevertheless, please check that the actual line voltage corresponds to the value set on these instruments! Never connect or disconnect any cable while the data acquisition and control electronics are ON. -
Page 3: Introduction
Single - Channel SPAD Detection Unit Manual 1. Introduction This manual describes the basic components of the single channel SPAD detection unit, as well as operation, maintenance and adjustment of the detectors . The Single Channel Detection Unit is an external detection unit for standard confocal Laser Scanning Microscopes (LSMs). -
Page 4: Physical Properties And Installation Requirements
Instrument response function between 350 and 800 ps FWHM dependent on module, wavelength and signal rate • Built-in thermoelectrical cooler with controller, signal amplifier and discriminator • Power supplied by the PicoQuant DSN 102 module Fig. 3.1: -SPAD detector module from PicoQuant Page 3... - Page 5 PicoQuant GmbH Single - Channel SPAD Detection Unit Manual PDM Series Single Photon Avalanche Diode from MPD (MPD SPAD) • Highest detection efficiency of 45 % at 530 nm • The active area in a mounted module has 100 µm diameter.
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Page 6: Set Up Of The Single Channel Detection Unit
➔ the count rate of one selected module. Power connectors are provided for -SPAD and MPD module types. Contact PicoQuant, if you ➔ want to change the detector type in your system. Because excessive light exposure will damage the SPAD module, we have equipped the DSN ➔... -
Page 7: Operation
PicoQuant GmbH Single - Channel SPAD Detection Unit Manual Fig. 4.2: DSN 102 dual SPAD power supply (optional) • At the DSN 102 (optional) select the appropriate input resistance (impedance) for the signal input with the input termination switch. Choose 1 MOhm if the signal is fed through this device to another device with an appropriate 50 Ohm termination. -
Page 8: Switching Off Your System
1. To check the general performance, acquire images with the normal LSM detectors and afterwards using the PicoQuant detectors. Thus a drop in performance can be easily recognized. In the following description we assume that LSM images using the standard LSM detectors can be obtained without problems, using reasonable settings. -
Page 9: Detector Unit Adjustment
7. Remove the cover from the Single Channel SPAD Detection Unit. 8. Adjust the laser settings and the axial position of the objective on the microscope until you detect a reasonable count rate (e.g. - Page 10 -SPAD detector upon scanning a fluorescence beam over the detection area by moving the focussing lens. 11. For an -SPAD detector from PicoQuant however, the maximum signal rate is only one of the criteria to optimize the lens position. In a second step, you have to position the focus close to the center of the active area of the SPAD to ensure the narrowest IRF (i.e.
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Page 11: Fundamental Alignment Of The Single Channel Spad Detection Unit
Generally, this alignment procedure should be a rare event. During the installation of the system by PicoQuant, the optics is aligned and then remains stable for a long time. Re-alignment is necessary when the LSM Upgrade Kit detector unit is disassembled, or when its optical design is changed. - Page 12 (Fig. 4.1) Fig. 5.7: Single Channel SPAD detection unit with empty filter holder being placed in the appropriate position. For fundamental alignment, the tubing indicated by the white arrow has to be removed. The hand control button (black arrow) can be pressed to open the shutter.
- Page 13 PicoQuant GmbH Single - Channel SPAD Detection Unit Manual Fig. 5.8: SPAD with removed tubing and attached beam alignment tool. Fig. 5.9: Fiber outcoupling of the Single SPAD Detection Unit with protection cap and coupler without protection cap (inset). The adjustment screws for tilting the beam are indicated in green, the counter screws for stabilizing the coupler position are indicated in yellow.
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Page 14: Recommended Literature
Wahl M.: Time tagged time resolved fluorescence data collection Numerous measurement examples are published on the PicoQuant website. Please visit the LSM Upgrade Kit section of http://www.picoquant.com/_systems.htm . This manual frequently refers to information in additional PicoQuant manuals, which also belong to the LSM Upgrade Kit documentation: •... -
Page 15: Useful Www Resources
PicoQuant GmbH Single - Channel SPAD Detection Unit Manual 7. Useful WWW Resources Optical filter manufacturers: • http://www.chroma.com • http://www.omegafilters.com • http://www.semrock.com • http://www.lambda.cc Fluorescence dyes, probes and labels: • http://www.probes.com (Alexa dyes) • http://www.amershambiosciences.com (Cy dyes) • http://www.exciton.com •... -
Page 16: Abbreviations
PicoQuant GmbH Single - Channel SPAD Detection Unit Manual 8. Abbreviations British Naval Connector or Bayonet Nut Connector or Bayonet Neill Concelman Charge-Coupled Device Constant Fraction Discriminator Counts per Second Continous wave (not pulsed) Fluorescence Correlation Spectroscopy Fiber Coupling Unit... -
Page 17: Support
Your feedback will help us to improve the product and documentation. In any case, we would like to offer you our complete support. Please do not hesitate to contact PicoQuant if you would like to have assistance with your system. - Page 18 WEEE-Reg.-Nr. DE 96457402 All trademarks mentioned in this manual are the property of their respective owners. PicoQuant claims no rights to any such trademarks used here. Products and corporate names appearing in this manual may or may not be registered trademarks or copyrights of their respective owners. They are used here only for identification or explanation and to the owner’s benefit, without intent to infringe.
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