Related Manuals for PicoQuant Single Channel PMA Hybrid Detection Unit
Summary of Contents for PicoQuant Single Channel PMA Hybrid Detection Unit
- Page 1 Single Channel PMA Hybrid Detection Unit External Detection Unit for Laser Scanning Microscopes User's Hardware Manual and Technical Data Version 1.4...
- Page 2 PicoQuant GmbH Single - Channel PMA Hybrid Detection Unit Manual Page 2...
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Page 3: Table Of Contents
1.1. Safety labels on the system......................... 5 2. Introduction..............................6 3. Physical Properties and Installation Requirements..................7 4. DETECTOR TYPE............................7 5. Set up of the Single Channel Detection Unit....................8 6. Adjusting the CFD threshold setting......................8 7. Operation..............................9 7.1. Switching Your Single Channel Detection Unit On................9 7.2. Switching Off Your System........................ 10 7.3. Checking Instrument Performance..................... 10 8. Further Reading............................11 8.1. PicoQuant Bibliography........................11 8.2. Download of Technical Notes / Application Notes................11 8.3. Recommended Websites........................11 9. Abbreviations............................. 12 10. Support..............................13 10.1. Returning Products for Repair......................13 10.2. PicoQuant Community Forum......................13 10.3. Problems and solutions........................13 11. Legal Terms............................. 14 11.1. Disclaimer............................14 11.2. Copyright............................14 11.3. Trademarks............................ -
Page 4: Safety Instructions
PicoQuant GmbH Single - Channel PMA Hybrid Detection Unit Manual 1. Safety Instructions LASER Warning! Lasers for two photon excitation deliver infra red emission which is in most cases not visible for the eye. These lasers emit light of laser class 4 / IV. Laser class 3B / IIIb and 4 / IV requires to wear special laser safety glasses. Do not remove or change any items of the Single Channel Detection Unit, especially the filter, before switching the microscope and laser completely off (see Fig. Fehler: Verweis nicht gefunden). The installation room of the LSM Upgrade kit must be labeled as laser area. For class 3B / IIIb and 4 / IV lasers, a laser safety officer has to be announced to meet the laser safety regulations. -
Page 5: Safety Labels On The System
To avoid hazardous radiation exposure you should carefully obey the safety instructions that are provided with your laser scanning microscope and laser manual. If your instrument uses another excitation system, follow the safety instructions of the relevant manual. The delivered instruments are pre-set by PicoQuant to operate on the power outlet line voltage for the country of delivery. Nevertheless, please check that the actual line voltage corresponds to the value set on these instruments! Never connect or disconnect any cable while the data acquisition and control electronics are ON. Charged signal cables can destroy the devices! -
Page 6: Introduction
NDD using a light guide. The PMA Hybrid detector allows time-resolved measurements with high sensitivity. This feature cannot only be used in FLIM applications, but also allows a broad range of FCS measurements. Possible applications are: FLIM and FLIM - FRET measurements with single molecule sensitivity • FCS measurements (autocorrelation) • Look at PicoQuant's website to download Technotes (http://www.picoquant.com/appnotes.htm ) regarding different techniques. Refer to your LSM manual for instructions on how to perform different measurements with your confocal microscope. -
Page 7: Physical Properties And Installation Requirements
PicoQuant GmbH Single - Channel PMA Hybrid Detection Unit Manual 3. Physical Properties and Installation Requirements SIZE [CM] (WIDTH ´ DEPTH ´ 6 ´ 24´ 14 HEIGHT) WEIGHT [KG] 5 KG OPERATION VOLTAGE 115V - 230 V AC FIBER CONNECTOR FC-APC TEMPERATURE: < 30°C ROOM REQUIREMENTS REL. HUMIDITY: < 60% SHOULD BE KEPT WITHIN 1°C, OTHERWISE TEMPERATURE VARIATION DEADJUSTMENT OF THE OPTICAL ELEMENTS CANNOT BE EXCLUDED 4. DETECTOR TYPE PMA Hybrid Suitable for the wavelength range from 300 nm to 720 nm • Provides high detection efficiency (typical 40% at 500 nm) •... -
Page 8: Set Up Of The Single Channel Detection Unit
PicoQuant GmbH Single - Channel PMA Hybrid Detection Unit Manual 5. Set up of the Single Channel Detection Unit Fiber / lightguide Detector protection Detector interlock interlock Fig. 1.2: Description of the Single Channel Detector In front of the PMA Hybrid, a filter can be placed into the filter holder (25 mm or 1 inch diameter). • Do not remove or change the filter of the Single Channel Detection Unit, before switching the microscope and laser completely off (see Fig. Fehler: Verweis nicht gefunden) -
Page 9: Operation
PicoQuant GmbH Single - Channel PMA Hybrid Detection Unit Manual Fig. 6.1: Adjustment of the CFD threshold level by using the SmyPhoTime64 software The optical fiber or the light guide should be attached to the microscope. No light should enter the • fiber or light guide for NDD. In case of a light guide, switch the room light off. The room must be completely dark. Please make sure that the detector is switched on and a filter is inserted into the filter holder in front • of the PMA Hybrid (see Fig. Fehler: Verweis nicht gefunden). While switching the PMA Hybrid on, both lamps at the rear side should be on. They should switch off after some time. Now the detector is ready. Start a time trace measurement. • Hydraharp and PicoHarp TCSPC: Set the CFD level to 100 mV, as displayed in Fig. 6.1. • TimeHarp and MultiHarp TCSPC: Set the CFD level to - 100 mV. • The final setting should be 10 mV higher compared to the setting, at which the count rate starts to • strongly increase. Please save this setting and restart the software in the standard user mode. •... -
Page 10: Switching Off Your System
6. Guide the detection light into the fiber exit port of the LSM. 7. Now the system is ready to take FLIM images or to make point measurements. For detailed description about how to perform individual measurements, follow the instructions in the LSM manual. 7.2. Switching Off Your System 1. Switch the detector off at the power distribution board (Fig. 7.1). 2. Turn off all other equipment, which is no further needed. 7.3. Checking Instrument Performance 1. To check the general performance, acquire images with the normal LSM detectors and afterwards using the PicoQuant detectors. Thus a drop in performance can be easily recognized. In the following description we assume that LSM images using the standard LSM detectors can be obtained without problems, using reasonable settings. 2. FCS setup only: A good way to check the performance of the detection unit is the acquisition of FCS- traces of a nM solution of a dye that can be effectively excited (see LSM manual, only for FCS systems). Acquire such traces with a standard solution from time to time using always the same settings (laser intensity, filters, pinhole size, objective, and the distance from the focus and the cover slide). If you are using a water objective, make sure the the cover slide correction ring is always at the same position. The count rate should not exceed 500 kHz, as the detector response becomes non linear at higher count... -
Page 11: Further Reading
PicoQuant GmbH Single - Channel PMA Hybrid Detection Unit Manual 8. Further Reading 8.1. PicoQuant Bibliography PicoQuant maintains a database of publications mentioning PicoQuant devices. It can be found at our web- site http://www.picoquant.com/biblio.php. It is a valuable source if you would like to know which laboratories are using PicoQuant products or how broad the field of various applications is. 8.2. Download of Technical Notes / Application Notes PicoQuant, along with our customers, continuously writes and publishes short documents about techniques, methods and applications that are possible with our hardware or software. The download section can be found at http://www.picoquant.com/appnotes.htm 8.3. Recommended Websites Optical filter manufacturers: http://www.chroma.com • http://www.omegafilters.com • http://www.semrock.com • http://www.lambda.cc • Fluorescence dyes, probes and labels: ... -
Page 12: Abbreviations
PicoQuant GmbH Single - Channel PMA Hybrid Detection Unit Manual 9. Abbreviations British Naval Connector or Bayonet Nut Connector or Bayonet Neill Concelman Charge-Coupled Device Constant Fraction Discriminator Counts per Second Continous wave (not pulsed) Fluorescence Correlation Spectroscopy Laser Coupling Unit FIFO First In, First Out (buffer type) FLIM Fluorescence Lifetime Imaging FRET Förster Resonance Energy Transfer FWHM Full-Width at Half-Maximum Input-Output Instrument Response Function Light Emitting Diode Laser Scanning Microscope Multichannel Scaling Non Descanned Detection Optical Density Personal Computer Peripheral Component Interface Pulsed Interleaved Excitation Photomultiplier Tube Red-Green-Blue (colour scheme) Region of Interest SubMiniature version A (connector type) Single Molecule Detection Photo-Multiplier Assambly SYNC Synchronization (signal) TCSPC Time-Correlated Single Photon Counting Transistor-Transistor Logic TTTR Time-Tagged Time-Resolved... -
Page 13: Support
If you observe any errors or bugs, please try to find a reproducible error situation. E-mail a detailed description of the problem and relevant circumstances to info@picoquant.com. Your feedback will help us to improve the product and documentation. In any case, we would like to offer you our complete support. Please do not hesitate to contact PicoQuant if you would like to have assistance with your system. Of course, we also appreciate good news: If you have obtained exciting results with the LSM Upgrade Kit or published scientific papers, we would also like to know! Please send us an e-mail to info@picoquant.com containing the appropriate citation. Gain additional publicity! PicoQuant maintains a database of publications mentioning PicoQuant devices and/or written by us. It can be found at our website at http://www.picoquant.com/_scientific.htm. It is a valuable source if you want to know which laboratories are using PicoQuant products or how broad the field of various applications is. Thank you very much in advance for your kind cooperation! Page 13... -
Page 14: Legal Terms
PicoQuant GmbH Single - Channel PMA Hybrid Detection Unit Manual 11. Legal Terms 11.1. Disclaimer All information given here is reliable to the best of our knowledge. However, no responsibility is assumed for possible inaccuracies or omissions. Specifications and external appearance are subject to change without notice. 11.2. Copyright Copyright of this manual and on-line documentation belongs to PicoQuant GmbH. No parts of it may be reproduced, translated or transferred to third parties without written permission of PicoQuant 11.3. Trademarks All trademarks mentioned in this manual are the property of their respective owners. PicoQuant claims no rights to any such trademarks used here. Products and corporate names appearing in this manual may or may not be registered trademarks or copyrights of their respective owners. They are used here only for identification or explanation and to the owner’s benefit, without intent to infringe. Page 14... - Page 15 PicoQuant GmbH Single - Channel PMA Hybrid Detection Unit Manual Retraction of old devices Waste electrical products must not be disposed of with household waste. This equipment should be taken to your local recycling centre for safe treatment. WEEE-Reg.-Nr. DE 96457402 PicoQuant GmbH Rudower Chaussee 29 (IGZ), 12489 Berlin, Germany Telephone: +49 / (0)30 / 1208820-0 Fax: +49 / (0)30 / 1208820-90 e-mail: info@picoquant.com www: http://www.picoquant.com Page 15...
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