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BEL Engineering INV-100 Series User Manual

Inverted biological microscope
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Inverted Biological
Microscope
Model
INV-100 series
User Manual
This manual is written for Inversed Biological Microscope INV-100. For safety and for
exerting the best performance, making you familiar with the instrument entirely, it is
strongly recommended that you read this manual carefully before using the microscope. For
the further reference, please place this manual in a position where nearby the worktable and
fetched easily.

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Summary of Contents for BEL Engineering INV-100 Series

  • Page 1 Inverted Biological Microscope Model INV-100 series User Manual This manual is written for Inversed Biological Microscope INV-100. For safety and for exerting the best performance, making you familiar with the instrument entirely, it is strongly recommended that you read this manual carefully before using the microscope. For the further reference, please place this manual in a position where nearby the worktable and fetched easily.

  • Page 2: Use Notices

    Use Notices INV-100 I、 Safety Note 1、 Do not keep the instrument in a direct sunlight, high temperature or humidity, dusty and easy shaking environment. Make sure the stage is plane, horizontal and stable enough. 2、 When moving the microscope, please hold up the instrument with one hand on the lower side of the eyepiece tube ①...
  • Page 3 lamp cools down enough before you do so. Ⅲ Safety Symbol Symbol Meaning The surface is very hot, not touch by your hands Before using ,please read the instruction carefully, improper operation will result in bodily injure or instruction malfunction. The main switch on The main switch off 3/28...
  • Page 4 1. Components INV-100 Objective Lamp house Phase contrast slider   10X（PH）  20X（PH）  Illumination bracket Ocular (eyepiece) Condenser Trinocular viewing tube Stage inserted plate (fixed) Objective turret (Nosepiece) Stage (fixed) (fixed) Microscope base Quintuple  Stage lengthen splint turret ...

  • Page 5: Installation

    2．Installation INV-100 2.1 Installing diagram The following figure shows the installation sequence of the components. The number in the figure shows the installation steps.  Before installing, be sure every components is clean, do not score any parts or glass surface. ...
  • Page 6 INV-100 2.2 Installing steps 2-2-1 Installing and replacing the lamp （figure3）  Please use the specified halogen Lamp 6V30W. Hold to the bulb ①after you wrap it with gauze or other protection materials, then depress the plugs② into the jack③ on the lamp house, ensure the filament and the bolt④...
  • Page 7 2-2-2 Installing the condenser illumination unit（figure 4） Insert the condenser illumination unit① into the bracket② gently, according the figure showed in the left. Turn the condenser illumination unit at clockwise about 90°, let the “AS” mark of filter holder③ facing forwards, and keep the screw of condenser illumination unit and the hole of the holder in line, then screw down the bolt in the hole with the supplied hexagon spanner.
  • Page 8 INV-100 2-2-4 Installing the objective（Fig. 6 and Fig.7） 1. Turning the coarse fusing knob① like the figure shows till the nosepiece get to its lowest position. ◐For ensuring safety instruction on transportation, the nosepiece is located in the lowest position and the tension adjustment collar②...
  • Page 9 2-2-5 Installing the stage lengthen splint and the mechanical ruler（fig. 8） ◎ Stage lengthen splint can be installed in either side of the stage to enlarge the work surface. But you can’t install the mechanical ruler together. ◎ Generally , the mechanical ruler will be installed in the right side for comfortable adjustment. Installing the stage lengthen splint First, Screw the fixed bolt①...
  • Page 10 INV-100 2-2-6 Installing the stage inserted plate（fig.9） 1. When using the glass stage ①, there is no special require, you just need to place it in a plane. 2. Install the stage inserted plate on to the stage opening. ◎ Turn the disk, let the V nick to face user, so the recognition of the objective will become easier.
  • Page 11 INV-100 2-2-9 Connecting the power cord（fig.12、13 and 14） ◐ Do not bring the power cord to bear a powerful stress. When being bent or wrapping, the cable and wires will be broken easily. 1. Turn the main switch① on “O”(off) state before connecting the power cord.
  • Page 12 3. Adjustment INV-100 Color filter holder Aperture diaphragm adjust lever Phase center bolt Phase contrast slider Diopter ring Light path selector lever Light switch Main switch Coarse focus knob Fine focus knob Tension adjustment collar Figure 15 12/28...

  • Page 13: Operating The Adjustment

    4. Operating the adjustment INV-100 4-1 Microscope base 4-1-1 Turning on the lamp （fig.16） Connect the power, turn on the main switch①(shown on the fig.16)which on the bottom side of the base to “－”(on). 4-1-2 Adjusting the brightness （fig.17） Turning the brightness adjustment knob clockwise, the voltage raise, and the brightness strengthen;...
  • Page 14 INV-100 4-2 Stage 4-2-1 Setting the specimen （fig.19and fig.20） Set the specimen in the center of the stage, please. ◐ to obtain the best observe effect, please select the containers, such as culture dish and culture bottle, with the bottom thickness is 1.2mm, and the same thickness is also required by the object slide when it is laid the specimen.
  • Page 15 ◐ be careful when you replace the objective, please, especially after a short work distance observation. Not let the objective to touch the stage inserted plate or the culture dish bracket. INV-100 4-3 the viewing tube 4-3-1 Adjusting the diopter（fig.21） Look into the right ocular by your right eye, then revolving the coarse focus knob to focus on the specimen.
  • Page 16 4-3-3 Switching the light path（fig.23） ◎ Pulling out the light path selector lever① by your thumb, select the light path you needed. ◎ when in the binocular observation, pushing in the lever until you heard a “clicked” .while in video or photography, pulling out the lever until it reached the “clicked”...
  • Page 17 INV-100 4-4 Illumination Unit 4-4-1 Using color filters（fig.24） ◎ Selecting the appropriate color filters according your need, it became more effective to observe or photography the specimen. Especially, we suggest using color filter, which compensate more neutral colors. ◎ You could pile up a group of color filters to the filter holder, if you ensure they are level and the whole thickness is less than 11mm.

  • Page 18: Phase Contrast Viewing

    4-4-2 using the aperture diaphragm（fig.25） ◎ When in the bright field observation, the aperture diaphragm control the numerical aperture of the illumination system. Only when the numerical aperture of the objective and the illumination system being matching, you can obtain the higher image resolution and contrast, and the increased depth of field, too.
  • Page 19 INV-100 5-2 The installation and use 5-2-1 Installing the phase contrast slider（fig.28） 1. Keep the slider① face ( the surface which had character) up towards, then inserted it into the illumination system from the right to the left like the figure showed. Every light ring or opening has its own located position, so you need to move them until you heard the “clicked”...
  • Page 20 4. Using the CT to look the light ring’s image① and the phase contrast ring’s image②, if the light ring’s image is not sharp, please shifting the CT’s ocular until you can see a clear image of the light ring②. 5.
  • Page 21 6．Microscope photography and video INV-100 6-1 Microscope video 6-1-1 selecting the light path（fig.31） ◐ just used in the trinocular observation Pulling out the light path selector lever, until you heard the “clicked”. ◐ In the dark specimen observation, you can make the focus by both eyes at first, then change the light path.
  • Page 22 INV-100 6-2 Microscope photography 6-2-1 selecting the light path ◐ just used trinocular observation The operation diagram is shown in 6-1-1， and the details reference is in 4-3-4。 camera 6-2-2 Installing the photography set （fig.33） 1. Loosen the locking bolt① on the trinocular viewing tube, and take out the dust cap②.

  • Page 23: Technical Specifications

    6-2-3 Focus Do the binocular observation at 20% brightness, and focus primary. When in microscope photography, do use the camera viewfinder to focus the specimen. Please refer the user manual of the camera set to obtain the details. 6-2-4 Adjusting the color temperature ◎...

  • Page 24: Troubleshooting

    7-2Objective Specifications COVER NUMERICAL WORKING CONJUGATE FOCUS SLIP TYPE APERTURE DISTANCE DISTANCE DISTANCE MAGNIFICATION THICKNESS （N.A） （mm） （mm） （mm） Infinite ∞ ─ 25.2 Long Working Distance ∞ Plan 1.2mm Achromatic Objective Infinite ∞ 0.17 0.25 Long Working Distance ∞ 0.17 Plan Phase Contrast Objective...
  • Page 25 The phase contrast slider is not Turn slider into “clicked” position located in the proper position There are stains on the Change a clean specimen 3. Find dust and specimen stain in the field There are stains and dust Clean the eyepiece of view on the eyepiece appear double...
  • Page 26 INV-100 SOLUTION PROBLEM REASON PAGE II、Mechanical Part: The tension adjustment 1.The coarse focus Loose properly collar is too tight knob is hard to run 2.The image can’t stay on the focal The tension adjustment Tighten properly plane in the process collar is too loose of the observation III.Electric Part:...
  • Page 27 around the image achromatic objective used achromatic objective 3.The indoor The extra light entered into the Cover up the eyepiece and the window eyepiece and viewfinder is viewfinder of the microscope fluorescence lamp reflected illumination system develop 27/28...
  • Page 28 28/28...

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