Page 1 NightSHADE evo LB 985N In vivo Imaging System with indiGO Software Operating Manual 73037BA2 Rev. No.: 00, 09/2022...
Page 2 Prefatory Comments LB 985N NightSHADE evo...
Page 3 The information in this guide is subject to change without notice. DISCLAIMER TO THE EXTENT ALLOWED BY LAW, BERTHOLD TECHNOLOGIES AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
Page 4 Prefatory Comments LB 985N NightSHADE evo...
Page 5: Table Of Contents
LB 985N NightSHADE evo Prefatory Comments Contents PREFATORY COMMENTS History of Changes Typographical conventions Warning signs at the instrument SAFETY INSTRUCTIONS Consignes de Sécurité Sicherheitshinweise WARRANTY AND CUSTOMER SERVICE Warranty Statement Customer Service QUICK START Installation First Acquisitions Image Processing and Evaluation Functions Creating a Report Using the Scheduler SYSTEM DESCRIPTION...
Page 6 Prefatory Comments LB 985N NightSHADE evo 10.2 Index 73037BA2 Rev. 00, 09/2022...
Page 7: Prefatory Comments
LB 985N NightSHADE evo Prefatory Comments 1 Prefatory Comments 1.1 History of Changes Revision Date Chapter / Page Change Author Rev. 00 09/2022 Initial release R. Kembügler 1.2 Typographical conventions The following typographical conventions will be used in this operating manual: Caution! Danger! Important information Example...
Page 8 Prefatory Comments LB 985N NightSHADE evo 73037BA2 Rev. 00, 09/2022...
Page 9: Safety Instructions
LB 985N NightSHADE evo Safety Instructions 2 Safety Instructions Caution! This sign alerts you to important operating procedures with a poten- tial danger of damaging the equipment and endangering your safety on dis- obeying. Refer to the user and instrument manuals for precautionary instruc- tions.
Page 10 Safety Instructions LB 985N NightSHADE evo BERTHOLD TECHNOLOGIES assumes no liability for any damages, including ❑ those to third parties, caused by improper use or handling of the instrument. ❑ The user is responsible for connecting the instrument in accordance with the valid regulations for electrical instruments.
Page 11 LB 985N NightSHADE evo Safety Instructions ❑ When the lid is opened (e.g. filter change) ESD can no longer be guaranteed. To avoid any damages to the electronic parts it is recommended to take pre- cautions (touching the metal case of a safety grounded object, wearing a grounding strap, etc.).
Page 12: Consignes De Sécurité
Les appareils ne doivent être utilisés que pour l'usage prévu. ❑ ❑ Berthold Technologies n'assume aucune garantie, y compris pour les dommages causés à des tiers, en cas de manipulation non conforme des appareils. Les appareils ne doivent être utilisés qu'à l'intérieur de locaux fermés ❑...
Page 13 La batterie interne ne peut être remplacée que par le même modèle ou par un modèle de remplacement recommandé ( ID 17391) par une personne autorisée par Berthold Technologies. Les piles usagées doi- vent être éliminées conformément aux instructions du fabricant.
Page 14 Safety Instructions LB 985N NightSHADE evo Installez l'appareil de manière à pouvoir le mettre en marche et ❑ l'arrêter facilement. Si la sécurité de fonctionnement est compromise, les appareils doivent ❑ être éteints et débranchés. Si du liquide a pénétré à l'intérieur de l'appareil, débrancher la fiche ❑...
Page 15: Sicherheitshinweise
❑ Transportsicherungen vor dem Einschalten entfernen. Die Geräte dürfen nur für den vorgesehenen Zweck eingesetzt wer- ❑ den. Berthold Technologies übernimmt keinerlei Gewährleistung, auch für ❑ Schäden gegenüber Dritten, die durch unsachgemäße Handhabung der Geräte hervorgerufen werden. ❑ Die Geräte dürfen nur innerhalb geschlossener Räume betrieben wer- den (trockene Umgebung).
Page 16 Safety Instructions LB 985N NightSHADE evo Die Geräte sind dafür ausgelegt, innerhalb des Temperaturbereiches ❑ von 10 bis 28 °C betrieben zu werden. Höhere Umgebungstempera- turen beeinträchtigen die Kühlung der Kamera (Sicherheitsabschal- tung der Kamerakühlung). Die Geräte sind dafür ausgelegt, bei einer maximalen relativen Luft- ❑...
Page 17 LB 985N NightSHADE evo Safety Instructions Stellen Sie das Gerät so auf, dass Sie es leicht ein- und ausschalten ❑ können. Bei Beeinträchtigung der Betriebssicherheit sind die Geräte abzu- ❑ schalten und vom Netz zu trennen. Ist Flüssigkeit in das Innere des Gerätes gelangt, Netzstecker ziehen. ❑...
Page 18: Warranty And Customer Service
Berthold Technologies shall in no event be liable or responsible for any incidental or conse- quential damage, either direct or indirect.
Page 19: Customer Service
Customer service will be provided in the first instance by the network of Berthold Technolo- gies representatives. In the event of any problem experienced with your instrument, the first recourse should be your local Berthold Technologies representative. For further problems re- quiring hardware or software expertise, the Technical Support group at Berthold Technolo- gies GmbH &...
Page 20: Quick Start
Quick Start LB 985N NightSHADE evo 4 Quick Start These quick start instructions provide a quick access to the program. You are guided step by step through the major program functions. The page numbers in the headers refer to the individual sections in the manual that provide more detailed information about the respec- tive functions.
Page 21: Installation
LB 985N NightSHADE evo Quick Start Installation 4.1.1 Requirements Please observe the following requirements when working with the NightSHADE evo: • 2-5 mains outlets (depending on accessories). The maximum power consumption of the NightSHADE evo without PC and external devices is max. 600 W. The power consumption of the external cooling unit is 340 W.
Page 22: Unpacking And Carrying
Quick Start LB 985N NightSHADE evo 4.1.2 Unpacking and Carrying The NightSHADE is delivered in a so-called flight case. The case consists of 3 parts, the lower base and two upper shells. The upper shells are locked to the base with butterfly locks and can be removed after releasing the locks.
Page 23: Camera Installation
LB 985N NightSHADE evo Quick Start 4.1.3 Camera Installation For safety reasons, the camera is delivered in a special case to prevent damage during transport. Therefore, the camera has to be installed first before it is taken into operation. Please note: Do not turn on the NightSHADE evo as long a no camera has been installed. The camera is connected to the instrument using two cables.
Page 24: Side View Camera
Quick Start LB 985N NightSHADE evo 4.1.3.2 Side view Camera The NightSHADE evo offers the special option that the camera can be mounted on the side, too. This feature enables the system to take images of plants from the side or to measure the sample dishes in an upright standing position.
Page 25: Connecting
LB 985N NightSHADE evo Quick Start 4.1.4 Connecting The power supply and device communica- tion ports are located on the rear panel of the NightSHADE evo. Plug the power cord into the mains inlet unit(1). The main power switch is located next to the mains inlet.
Page 26: Software Installation
Quick Start LB 985N NightSHADE evo 4.1.7 Software Installation The indiGO software is included on the enclosed USB stick. Use this media to install the software in order to use the correct individual calibration data which is automatically copied during installation. Keep this media in a secure place. You will need it again e.g.
Page 27 LB 985N NightSHADE evo Quick Start In this case the default systems filter management file will be copied to your com- puter. If you reinstall the software the current active systems filter management file will be overwritten. To get to the next screen asking for the drivers to be installed on the system click on When working with the NightSHADE evo the PCI drivers are not required.
Page 28: Instrument Controller First Time Initialization Setup
You can check the instrument ident-number by starting „Scan Devices“. If the calibration for your device was stored on the NightSHADE evo by Berthold Technologies before it was delivered you can download the stored calibration parameters from the instruments flash memory now by confirming „Yes“.
Page 29 LB 985N NightSHADE evo Quick Start This step of the NightSHADE evo Controller First Time Initialization Setup allows the im- port of further created configuration backups and calibration files. Select to continue. To maintain the instrument the instruments controller needs to create an administrator account.
Page 30 Quick Start LB 985N NightSHADE evo Select to continue. Next, choose a start method for the controller. . With regular installations it is recom- mended to select the first option (“Start controller with indiGO"). The indiGO controller takes care of the hardware control and performs the measurements even when indiGO itself is not running.
Page 31: First Time Initialization Setup
LB 985N NightSHADE evo Quick Start 4.1.9 First Time Initialization Setup To configure indiGO launch the software by double-clicking on the indiGO icon. The first time initialization setup will start. If the PC is directly conneted to the NightSHADE evo via USB choose “Server” to install the instrument controller as well as the evaluation part.
Page 32 Quick Start LB 985N NightSHADE evo The indiGO software is able to manage the database on the local computer as well as on a network location. Therefore the database location can be set up to an individual folder. The benefit of storage on the network is that one has access to the data from sev- eral evaluation stations, which is a major advantage in a multi-user environment.
Page 33 LB 985N NightSHADE evo Quick Start In case no user management is required tick the box <Disable User Manage- ment>. No log-in is required and no user management is available. If the User Management has been disabled it can be re-activated via the system configura- tion at any time.
Page 34: User Management
Quick Start LB 985N NightSHADE evo 4.1.10 User Management IndiGO supports an integrated user management. The User Management can be enabled or disabled by the system administrator. Refer to the <User Management> <Advanced> settings to enable or disable the user management. Only admins can access the user management.
Page 35: Installing Fluorescence Filters
LB 985N NightSHADE evo Quick Start calibration is denied. The access to measurements is restricted by the user management. Other users can be invited to own projects. Guest Guests can run measurements in projects where they have been invited to. 4.1.11 Installing Fluorescence Filters Excitation Filter Slider The NightSHADE evo is equipped with automatic filter changers for the excitation and emis-...
Page 36 Quick Start LB 985N NightSHADE evo To eject the slider select When loading the filter please observe the correct installation direction (see above). To re- load the slider, select . After that the slider is initialized by the NightSHADE evo. Emission Filter Wheel The emission filters are located on a filter wheel in front of the lens.
Page 37: Filter Management
LB 985N NightSHADE evo Quick Start 4.1.12 Filter Management indiGO offers an integrated filter management. The filter management is used to describe the filter load on the excitation filter sliders as well as the emission filter wheels. The system comes with a set of GFP filters. The recommended excitation position for the GFP exciter (478/20) is position 1.
Page 38: Filter Inventory
Quick Start LB 985N NightSHADE evo 4.1.13 Filter Inventory The filter inventory is used to collect the available filters. indiGO governs two filter inventory lists. One is used for excitation filters, the other for emission filters. The inventory lists can be either reached via <Excitation Inventory> <Filter Inventory> for the excitations filters or via <Emission Inventory>...
Page 39: Filter Manager
LB 985N NightSHADE evo Quick Start 4.1.14 Filter Manager Once a filter has been added to the filter inventory list it can be used for an excitation filter slider or an emission filter wheel. The filter manager can be reached via <Excitation Inventory> <Filter Manager> for the ex- citations filters or via <Emission Inventory>...
Page 40: First Acquisitions
Quick Start LB 985N NightSHADE evo 4.2 First Acquisitions 4.2.1 Quick Start Glossary Projects, Applications and Identities: All parameters necessary to run a measurement are defined in an application. Individual samples, called identities, can be organized in a project. Each project is connected with an application.
Page 41: Launching The Software
LB 985N NightSHADE evo Quick Start 4.2.2 Launching the software Depending on the system configuration it might be required to log-in after the indiGO has been started. When no other users are defined the standard log-in is as follows: Log-in: Admin Password: Admin...
Page 42 Quick Start LB 985N NightSHADE evo Select to define the measurement workflow. Four different kinds of measurements can be performed. Please note that depending on the system configuration some of the selections might not be available (e.g. due to a missing fluorescence illumination module).
Page 43 LB 985N NightSHADE evo Quick Start background noise will be obtained. Low speed is ideal for highest sensitivity lumi- nescence measurements. Be aware that with low speed the camera readout can take up to 15 seconds. • Binning Factor is the combinated readout of neighbouring CCD pixels. The result is an increased signal and thus an improved sensitivity and a better signal-to-noise ratio, however, at the cost of loss in spatial resolution.
Page 44 Quick Start LB 985N NightSHADE evo To close the luminescence parameter wizard select The summary of the new created luminescence measurement is shown in the right column: To add a photographic exposure to the list of measurements select <Add>, <Photo>. The default parameters (100ms exposure time, 10% illumination intensity) are suitable for most applications.
Page 45 LB 985N NightSHADE evo Quick Start For the sample size definition dialog box select You can either select an already existing format using the load button or define a new sam- ple format. The following parameters are required: • Sample Size: The sample size refers to the image size (width) in mm.
Page 46 Quick Start LB 985N NightSHADE evo To go to the next page select On this page you can define the setting for the “Region of Interest” as well as the “Multi Sample” parameters. By using the “Region of Interest” a section of the CCD chip can be defined to get faster framerates.
Page 47: Defining A Controller Template
LB 985N NightSHADE evo Quick Start In case of multi sample the required identities will be defined before starting a measure- ment. To close the application wizard select 4.2.4 Defining a Controller Template When creating a controller template the workflow is about the same as with a measuring- template.
Page 48 Quick Start LB 985N NightSHADE evo To turn on the LED illumination (Option) select <Artificial Daylight> from the list. Now the intensities of the different wavelength can be adjusted Adjust the required intensities and click on to accept the parameters. The control command shows up in the work flow list.
Page 49 LB 985N NightSHADE evo Quick Start the artificial daylight is turned back on to the previously used intensity after a measurement. • Illumination Controls the intensity of the white or infrared (option) LEDs in the dark room for photographic exposures. •...
Page 50 Quick Start LB 985N NightSHADE evo • Sample Changer (option) Moves the sample changer to a defined position. • Halogen This command turns on the excitation light of the fluorescence illumination.. • Positioning Plate (available with older NightSHADEs only) The positioning plate is an optional table to move the sample in X and Y direction. The required position can be entered in mm or a predefined position can be se- lected from the dropdown list.
Page 51: Defining A Project
LB 985N NightSHADE evo Quick Start 4.2.5 Defining a Project To define a new project refer to <Project & Analysis> <Available Projects>. Select <New Project > from the <Available Projects > context menu. Projects are used to define non measurement-specific parameters, like user access rights, scale settings, etc.
Page 52 Quick Start LB 985N NightSHADE evo On this page the number of sample identities and the sample names can be defined. A prefix, the current year and month, a 3 digit sample ID counter and a suffix can be speci- fied if required.
Page 53 LB 985N NightSHADE evo Quick Start On the last page users can be assigned to the project. The projects creator automatically receives ownership of the project. Other users can be added to the project. To create new users refer to the indiGO user manager. All defined users are listed in the left column and can be added to the project using the add command ( ).
Page 54: Starting An Acquisition
Quick Start LB 985N NightSHADE evo 4.2.6 Starting an Acquisition The system can carry out measurements on demand or scheduled using a time table. First we will show here how to create an acquisitions on demand. Further details about how to use the scheduler can be found at the end of the chapter.
Page 55 LB 985N NightSHADE evo Quick Start To start a measurement select . Upon completion of the measurement, the data is automatically stored. The data is displayed by double clicking on the sampleID. The measurement consists of a photographic image in grayscales and the luminescence image in pseudo color.
Page 56: Image Sequences
Quick Start LB 985N NightSHADE evo 4.2.7 Image Sequences In addition to single measurements indigo also offers automatic sequence measurements. These kind of measurements allow automatically triggered measurements in a predefined schedule. To start a sequence measurement refer to <Acquisition & Scheduling>, <Create a new Sched- ule>.
Page 57 LB 985N NightSHADE evo Quick Start Clicking on leads to the following dialog box. In this dialog box the starting time for the measurement- or control sequence can be de- fined. Alternatively “Start right away” can be selected by checking the box. This will enable an immediate start when is clicked.
Page 58 Quick Start LB 985N NightSHADE evo Job details can be seen by doubleclicking the corresponding item in the list. To delete a single job, click on the item to select it and click on . To delete the whole Schedule click on 73037BA2 Rev.
Page 59: Image Processing And Evaluation Functions
LB 985N NightSHADE evo Quick Start 4.3 Image Processing and Evaluation Functions 4.3.1 Load Images During the first acquisition images have been created and stored in the project folder. To re-open the previously taken images refer to the project folder first and select the ap- propriate identity.
Page 60: Image Window
Quick Start LB 985N NightSHADE evo 4.3.2 Image Window After an acquisition or the opening of an identity the measurement and the photographic image are displayed. By default the photographic image is overlaid by the measurement or signal image using pseudocolors. At the left margin of the screen you see the intensity scale and histogram of the photographic image, together with the associated sliders.
Page 61 LB 985N NightSHADE evo Quick Start The system offers the possibility to zoom the histogram by using . The maximum range of the histogram is displayed on the zoom toolbar. By selecting <xxx> the histogram can be set to fullscale. With <xxx>...
Page 62 Quick Start LB 985N NightSHADE evo The following options are available: <Apply Image and Contrast settings> will apply the image and contrast settings from the current image to all other measurements in the project. If <Use current Settings as default for new Measurements> is selected, these settings are also valid for all future measurements in the project.
Page 63: Line Plot
LB 985N NightSHADE evo Quick Start 4.3.3 Line Plot For further analysis of the measurements it might be helpful to create a 1D-line plot of the measured data. This function is recommendet to evaluate the measuring data. To use this function select An additional window is opened where the line profile created from a given lead is dis- played.
Page 64: Surface Plot
Quick Start LB 985N NightSHADE evo 4.3.4 Surface Plot To get a 3D surface plot select <Surface> from the <Report> menu.The surface can be used for 3D visualization of the measured data. To create a 3D surface plot from the photographic image only please refer to associated command.
Page 65: Peaksearch
LB 985N NightSHADE evo Quick Start 4.3.5 Peaksearch Once the image has been loaded and the color view has been set up, you can start to analyse the evaluation. In the first step the desired areas has to be marked; now indiGO can calculate the intensity values.
Page 66: Define Areas Manually
Quick Start LB 985N NightSHADE evo None ▪ No discrimination method is being used. Every spot which is above the given lower threshold will be treated as peak. Advanced - Remove Areas inside Areas If this option is set areas which are located inside other areas will be declined. Maximum Areas This parameter defines the maximum amount of peaks.
Page 67 LB 985N NightSHADE evo Quick Start To define a freehand shape circle the desired area with several clicks on the left mouse but- ton or hold it down completely (pencil mode). A double-click will end the procedure. The painted shape will be connected automatically and the freehand shape will be closed by a frame.
Page 68 Quick Start LB 985N NightSHADE evo Further commands for the area management are available via the contect menu of the area items list. To export areas the <Export> command from the “Overlay Properties” can be used. To import areas the <Import> command from the “Overlay Properties” can be used. 73037BA2 Rev.
Page 69: Creating A Report
LB 985N NightSHADE evo Quick Start 4.4 Creating a Report 4.4.1 Creating a Rich-Text-Report indiGO offers the possibility to create and display measurement reports. The output can be adapted to the user's requirements. Using the <Rich-Text-Report> command in the <Report> menu will start the Rich-Text-Re- port-Creation-Wizard.
Page 70: Excel Export
Quick Start LB 985N NightSHADE evo emitted power (nW). The photons as well as the power values are besed on a calibration us- ing a light standard. This calibration is only valid, when the sample is placed on the base plate and not higher than 50mm.
Page 71 LB 985N NightSHADE evo Quick Start • Create a Front Page With this command you can create a report front page. The contents of the page, like your company name, address or logo can be setup through the indiGO options. •...
Page 72 Quick Start LB 985N NightSHADE evo The spreadsheet will contain one to three worksheets depending on the selected options: • IndiGO The “indiGO” sheet contains the front page • Analysis The “Analysis” sheet contains all measurement results and the diagrams, depending on the settings.
Page 73: Using The Scheduler
LB 985N NightSHADE evo Quick Start 4.5 Using the Scheduler Control sequences like sunset and sunrise or measurements for selected identities can be controlled via a scheduler. You can think of the scheduler as a simple list of time controlled events.
Page 74 Quick Start LB 985N NightSHADE evo When selecting <Schedule Controller Template> a dialog box pops up to select the desired control sequence. Chose to confirm the selected template and continue. On the next page the <Start Execution> time can be selected. In the sample above, the first measurement will be executed on Wednesday the 16th of May at 16:00:00.
Page 75 LB 985N NightSHADE evo Quick Start The scheduler shows the list of scheduled measurents and control commands with absolute starting time and relative time. The time of the first event in the list (when the scheduler was empty before) is treated as time zero. To highlight an item just click on it.
Page 77: System Description
LB 985N NightSHADE evo System Description 5 System Description Use and Function Design of the Acquisition Device Acquisition Chamber 5.3.1 Light-tight Door with Door Button 5.3.2 Illumination of the Acquisition Chamber (Dark Box) 5.3.3 Connector Field 5.3.4 Camera and Lens 5.3.5 Filter Wheel 5.3.6 Filter Slider 5.3.7 Fluorescence Illumination...
Page 78: Intended Use
This instrument is not designed or intended for use with installations or equipment in haz- ardous environments. Servicing of the instrument must only be performed by Berthold Tech- nologies Field Service Engineers or service staff authorized by Berthold Technologies. NightSHADE evo LB 985N...
Page 79: Design Of The Acquisition Device
LB 985N NightSHADE evo System Description 5.2 Design of the Acquisition Device The acquisition device includes the hardware and electronics required for image acquisition. It com- prises the acquisition chamber (dark box) including the camera lift with camera, lens and filter wheel. The electronics and excitation light source for fluorescence measurements are accommodated in the upper part of the device.
Page 80 System Description LB 985N NightSHADE evo Front side Light-tight door with handle to close the acquisition chamber Door for loading and unloading the filter slider for fluorescence emission Service flap for halogen lamp and excitation filter slider loading and unloading. Operating status indicator acquisition chamber Inside the...
Page 81: Acquisition Chamber
LB 985N NightSHADE evo System Description 5.3 Acquisition Chamber 5.3.1 Light-tight Door with Door Button To close the acquisition chamber light-tight, the door frame is provided with an elastic seal- ing and a handle which, when turned by 180°, will pull the door into the frame and squeeze it against the sealing.
Page 82: Camera And Lens
System Description LB 985N NightSHADE evo 5.3.4 Camera and Lens NightSHADE evo LB 985N uses a slow scan CCD camera, hermet- ically vacuum encapsulated and cooled down by a Peltier ele- ment to -70°C (the operating temperature is reached after about 10 minutes).
Page 83: Filter Slider
LB 985N NightSHADE evo System Description 5.3.6 Filter Slider The filter slider can accommodate five excitation filters with a diameter of 25 mm (or 1 inch). It is located under the flap on the right top side of the instrument. A light blocking knob keeps out light to ensure best performance.
Page 84: Fluorescence Illumination
System Description LB 985N NightSHADE evo 5.3.7 Fluorescence Illumination The built-in halogen light source together with the filter slider create the excitation light for flourescence measurements. The light is guided into the dark box to the ring light (1). For alternative illumination devices using standard 15mm fibre optic connectors an auxiliary light output port (2) is located at the right side at the ceiling of the dark box.
Page 85: Top Part Of Acquisition Device
LB 985N NightSHADE evo System Description 5.3.9 Top Part of Acquisition Device The upper part of the instrument contains the camera, the fluorescence light source (option) and the complete electronics. To open lift up the lid. Inside 2 connectors are visible. One is for the cmare power supplky, the other is the USB dataport to control the camera.
Page 87: Indigo Software Documentation
LB 985N NightSHADE evo indiGO Software Documentation 6 indiGO Software Documentation Graphical User Interface Application Toolbar Identity Context Identities and Measurements Contrast Slider 6.5.1 Photo Properties Quick Menu 6.5.2 Photo Properties Dialog Data Slider 6.6.1 Data Properties Quick Menu 6.6.2 Data Properties Dialog Toolbar IndiGO Menu 6.8.1 New...
Page 88: Graphical User Interface
indiGO Software Documentation LB 985N NightSHADE evo 6.1 Graphical User Interface The indiGO graphical user interface is clearly structured and offers a variety of setup options. Measurements are automatically displayed as overlay. 6.2 Application Toolbar On the toolbar you find the following commands: •...
Page 89: Identity Context
LB 985N NightSHADE evo indiGO Software Documentation • Quick Check Start the <Quick Check> utility to create a photo or run a measurement for test circum- stances. • Device State Open a window to display the current state of the NightSHADE evo. •...
Page 90 indiGO Software Documentation LB 985N NightSHADE evo • Apply to Identity With this command the current image and contrast settings, as well as the overlay prop- erties of the annotation and the manual layers can be applied to all measurements of the current identity.
Page 91: Identities And Measurements
LB 985N NightSHADE evo indiGO Software Documentation 6.4 Identities and Measurements • Top Row Currently opened sample identities • Center Row List of all measurements belonging to the current selected identity • Bottom Row Measurement or data toolbar 6.5 Contrast Slider With the contrast slider on the left side of the image the brightness of the photographic display is defined.
Page 92: Photo Properties Quick Menu
indiGO Software Documentation LB 985N NightSHADE evo 6.5.1 Photo Properties Quick Menu To access the drop down menu simply click on the arrow next to the icon shown below. • Reload Settings Reload the last stored settings for the scaling and the alignment. •...
Page 93 LB 985N NightSHADE evo indiGO Software Documentation Reference: real name photo displayed link. example: 201003 20100301005800.photo.000, where is the date the photo was taken. In our exam- ple: 03. March 2010. 005800 is the recording time. In our example: 12:58:00 AM. By fol- lowing the link, the indiGO image viewer will be opened and the photo will be displayed.
Page 94: Data Slider
indiGO Software Documentation LB 985N NightSHADE evo 6.6 Data Slider On the right margin of the screen you see the intensity scale of the measurement, together with the associated slider. With these two sliders you can set the scaling of the pseudocolor signal image and the lower threshold of the color view. The top slider defines the intensity value.
Page 95 LB 985N NightSHADE evo indiGO Software Documentation For further analysis a LUT and a surface is supported. In addition the overlay intensity can be set through <Overlay level>. 73037BA2 Rev. 00, 09/2022...
Page 96 indiGO Software Documentation LB 985N NightSHADE evo • Filter The Filter page can be used to change or setup the filter properties used to render the overlay. This might be useful when more than one signal layer must be displayed in order to assign different properties to each signal layer.
Page 97: Toolbar
LB 985N NightSHADE evo indiGO Software Documentation 6.7 Toolbar • Decrease the zoom Used to decrement the zoom • Increase the zoom Used to increment the zoom • Current zoom Used to setup the zoom manually, displays current zoom factor •...
Page 98 indiGO Software Documentation LB 985N NightSHADE evo • Manually (Menu) Create manual areas • Peak Search (Menu) Starts the peak search • Peak Search (Command) 73037BA2 Rev. 00, 09/2022...
Page 99 LB 985N NightSHADE evo indiGO Software Documentation • Overlay Properties (Dialog) The <Overlay Properties> dialog can be opened via the <Annotaion>, <Manually> or <Peak Search> commands • Line Plot Starts the Line Plot Tool • Report (Menu) • Report (Command) Starts the wizard to create an Excel report.
Page 100 indiGO Software Documentation LB 985N NightSHADE evo The output name and destination of the video must be entered. Clicking on leads to the following dialog The frames per seconds for the video can be entered here. Depending on the amount of sequence images this will define the length of the video. A codec must be selected.
Page 101: Status Bar
LB 985N NightSHADE evo indiGO Software Documentation 6.8 Status Bar In the status bar the current status of the hardware and software is shown. shows the current status of the system. When a measurement is running a progress bar is shown. displays the staus of the Scheduler.
Page 102: Indigo Menu
indiGO Software Documentation LB 985N NightSHADE evo 6.9 IndiGO Menu 6.9.1 New Project To create a new project, start the wizard. For further details please refer to section 4.2.5. Controller Template To create a new controller template, start the wizard. For further details please refer to section 4.2.4.
Page 103: Print Report
LB 985N NightSHADE evo indiGO Software Documentation 6.9.6 Print Report indiGO offers the possibility to create and display measurement reports. The output can be adapted to the user's requirements. For further details please refer to chapter 4.4 6.9.7 Logoff This command will change the current user account. When clicking on “Change password”...
Page 104: Tools Menu
indiGO Software Documentation LB 985N NightSHADE evo 6.10 Tools Menu 6.10.1 Backup Projects IndiGO can create backups of the database or selected projects. Backups are created using the backup smart wizard. The wizard will guide you through the steps necessary to successfully create a backup. When selecteing the Backup first define which projects have to be backed up.
Page 105: Import Winlight Data
LB 985N NightSHADE evo indiGO Software Documentation After launching the wizard just select the project file (.proj) of the indiGO 1.x project to import. The “Destination Project” will define the Project name where the imported files will be cop- ied to. An import project name cannot be used for data acquisitions. Clicking on will lead to a selection dialog box.
Page 106: Service Menu
indiGO Software Documentation LB 985N NightSHADE evo 6.11 Service Menu 6.11.1 Filter Manager This command will start the indiGO filter manager. For further details please refer to section 4.1.14 6.11.2 User Manager This command will start the indiGO user manager. For further details please refer to section 4.1.12.
Page 107: Device State
LB 985N NightSHADE evo indiGO Software Documentation The Instrument Controller Setup is used to setup your NightSHADE evo or NightOwl device and to perform all system calibration tasks. 6.11.4 Device State The <Device State> command can be used to display the current states of the instrument, the camera and the scheduler.
Page 108: Quick Check
indiGO Software Documentation LB 985N NightSHADE evo 6.11.6 Quick Check The <Quick Check> command can be used to get access to highlevel quickcheck commands for photo, luminescence and fluorescence. Each highlevel quickcheck command will start a smart wizard which will guide you through the neccessary steps to perform the quickcheck.
Page 109: Options
LB 985N NightSHADE evo indiGO Software Documentation 6.11.9 Options The Options command allows you to setup further indiGO options. Overlay Access to overlay colour options. Here user specific colors for the different data types (peak search areas, manual areas etc.) can be defined. IndiGO Access to setup configuration wizards and connectivity settings necessary for update and support mail access.
Page 110 indiGO Software Documentation LB 985N NightSHADE evo Setup Database Connection Using this wizard the connection type can be changed. This might be required when the computer was moved away from the system or in case the database was changed from the local disk to a network drive.
Page 111 LB 985N NightSHADE evo indiGO Software Documentation Export Export report settings like address, company logo and preferred format. 73037BA2 Rev. 00, 09/2022...
Page 112: View Menu
indiGO Software Documentation LB 985N NightSHADE evo 6.12 View Menu 6.12.1 Toolbar This command shows or hides the toolbar. This option is not persistent and the toolbar will be shown on the next start of indiGO. 6.12.2 Status Bar This command shows or hides the status bar. This option is not persistent and the statusbar will be shown on the next start of indiGO.
Page 113: Trouble Shooting
LB 985N NightSHADE evo Trouble Shooting 7 Trouble Shooting 7.1 Hardware 7.1.1 Door status always open If the door status is always open, the reason could be a disaligned door sensor. The door senssor is located at the upper right front side of the dark box. To adjust the door sensor refer to 8.2.3 7.1.2 Fluorescence lamp not working The built in halogen fluorescence excitation light source has a lifetime of approx.
Page 114: Cleaning And Maintenance
Cleaning and Maintenance LB 985N NightSHADE evo 8 Cleaning and Maintenance Camera and Lens 8.1.1 Removing the bottom plate 8.1.2 Cleaning the Dark Box 8.1.3 Cleaning the Camera 8.1.4 Cleaning the Lens Darkbox 8.2.1 Replacing the Air Filter 8.2.2 Replacing the Fuse 8.2.3 Setting the Door Sensor 8.2.4 Replacing the halogen lamp 73037BA2 Rev.
Page 115: Camera And Lens
LB 985N NightSHADE evo Cleaning and Maintenance 8.1 Camera and Lens 8.1.1 Removing the bottom plate The retractable bottom plate is removable. The bottom plate is locked by a latch underneath the telescopic rail. To remove the plate open the latch on both rails. The black plastic latch is located inside the rail.
Page 116: Darkbox
Cleaning and Maintenance LB 985N NightSHADE evo 8.2 Darkbox 8.2.1 Replacing the Air Filter The air filter should either be cleaned or replaced, depending on the level of contamination. Remove the black ventilator cover on the rear of the instrument and clean the filter (blow it out or wash it) or replace it.
Page 117 LB 985N NightSHADE evo Cleaning and Maintenance 8.2.4 Replacing the halogen lamp The halogen lamp is located close to the excitation filters. It is protected by a black cover plate. To replace the lamp proceed as follows. Make sure that the same type of lamp is used to guarantee proper light output.
Page 119: Technical Data
LB 985N NightSHADE evo Technical Data 9 Technical Data Camera Peltier/air-cooled CCD camera C-Mount adapter Resolution 1024 x 1024 Pixel x 13µ Quantum efficiency (max.) >90%. Noise Readout: Σ 7 e Exposure time from milliseconds to hours Binning of pixels Variable in X- and Y-direction to increase the signal/noise ratio Temperature Ambient temperature during operation: 10...
Page 120: Appendix
Appendix LB 985N NightSHADE evo Appendix 10.1 Accessories 10.1.1 LED Panels The LED panels are used to simulate environmental conditions like day / night changes while the sample is placed inside the dark box and no measurement is running. The LED panels are available in 2 different versions with the following wvelenght configu- ration: •...
Page 121 LB 985N NightSHADE evo Appendix blue green white far red The electrical and cooling liquid tubing connections inside of the dark box are shown in the image below: Connect the LED panels only while the instrument is powered off to prevent electrical damage of the LED panel.
Page 122 Appendix LB 985N NightSHADE evo Tubing connector at the miniChiller and at the rear panel of the NightSHADE evo When the miniChiller or any other device using liquids to cool the LED panel is connected to the NightSHADE evo it is not allowed to operate 100/230V devices such as transillumina- tors etc.
Page 123 LB 985N NightSHADE evo Appendix 10.1.2 Temperature Controlled Measurement Table The temperature controlled measurement table is used for measurements which require a temperature controlled environment. If you are working with the heated macro table, plug the connector into the 9-pole Sub-D- connector on the power box at the center top inside the darkbox (see 5.3.3.) The device must be switched off, since after the NightSHADE evo is switched on the heating system is de- tected and may get initialized.
Page 124 Appendix LB 985N NightSHADE evo 10.1.3 Temperature controlled tray with anti condensation The temperature controlled tray can be placed inside the NightSHADE evo to allow a tem- perature controlled base plate when using petri dishes. The temperature is regulated by the water cycle used by the LED panels as well.
Page 125 LB 985N NightSHADE evo Appendix Avoid temperatures who are significantly below ambient as this could lead to condensation. 10.1.4 Turntable The turntable is used together with the sideview option. Using the turntable up to 6 samples can be measured in a automatic sequence. 3 different sample holders can be selected: •...
Page 126 Appendix LB 985N NightSHADE evo • Connect the 9 pin cable supplied with the turn table to the connector at the con- necting panel at the upper position inside the dark box. Make sure the cable is not blocking the table or gets sqeezed by the retractable base plate. Remove the dummy plug and keep it in a save place.
Page 127 LB 985N NightSHADE evo Appendix 10.1.5 Test Light Source The Test Light Source is used to calibrate and test the System. It contains a low light source which will glow for about 15 min. without external supply. To charge the unit, use the included mains adapter. Charging is indicated by a red LED on the test plate.
Page 128 Appendix LB 985N NightSHADE evo 10.2 Index 1D-line plot filter wheel acquisition first time initialization Admin Flatfield Correction Air Filter Fluorescence Filters Anti condensation fuse Application Fuse Apply to Gain Identity Guest Project halogen lamp Areas Identities Manual Import Peaksearch indiGO 1.x Data Artificial daylight WinLight Data...
Page 129 LB 985N NightSHADE evo Appendix Modifications due to technical advancement reserved. 73037BA2 Rev. 00, 09/2022...

BERTHOLD TECHNOLOGIES NightSHADE evo LB 985N Operating Manual

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5 System Description

6 Indigo Software Documentation

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