Page 1 Rev. No.: 05 These units are not designed for use in hazardous areas. The units must not be repaired by any person other than Berthold Technologies service engineers or technicians authorized by Berthold Technologies. In case of operation trouble please contact your local service organization...
Page 2 Operating Manual LB 983 NightOWL II Berthold Technologies GmbH & Co.KG Calmbacher Str. 22 75323 Bad Wildbad Germany Phone +49 7081 177-0 Fax +49 7081 177-100 bio@Berthold.com www.Berthold.com/bio...
Page 3: Table Of Contents
LB 983 NightOWL II Operating Manual Contents Contents PREFATORY COMMENTS Typographical conventions Warning signs at the instrument SAFETY INSTRUCTIONS Consignes de Sécurité Sicherheitshinweise WARRANTY AND CUSTOMER SERVICE Warranty Statement Customer Service QUICK START Installation First Acquisitions Image Processing and Evaluation Functions Creating a Report SYSTEM DESCRIPTION Use and Function...
Page 4 LB 983 NightOWL II Operating Manual Contents 10.7 Index...
Page 5: Prefatory Comments
LB 983 NightOWL II Operating Manual Prefatory Comments Prefatory Comments 1.1 Typographical conventions The following typographical conventions will be used in this operating manual: Caution! Danger!  Important information Example Menus and options in the software are printed in bold type Measurement, View The selection of a menu item is written as a sequence of commands, separated by a vertical line...
Page 7: Safety Instructions
LB 983 NightOWL II Operating Manual Safety Instructions Safety Instructions Caution! This sign alerts you to important operating procedures with a po- tential danger of damaging the equipment and endangering your safety on disobeying. Refer to the user and instrument manuals for precautionary in- structions.
Page 8 LB 983 NightOWL II Operating Manual Safety Instructions  BERTHOLD TECHNOLOGIES assumes no liability for any damages, includ- ing those to third parties, caused by improper use or handling of the instru- ment.  The user is responsible for connecting the instrument in accordance with the valid regulations for electrical instruments.
Page 9: Consignes De Sécurité
LB 983 NightOWL II Operating Manual Safety Instructions  The system always has to be primed with solutions recommended by the kit manufacturer.  Use only reagents recommended by the kit manufacturer.  Use reagents only in accordance with the kit manufacturer’s instructions. ...
Page 10 31 °C et diminue linéairement jusqu'à 50% humidité relative pour une température d’ambiance allant jusqu’à 40 °C.  BERTHOLD TECHNOLOGIES décline toute responsabilité de dommages résultant d’une utilisation non conforme à l’emploi pré- vu, y compris les dommages causés à des tiers.
Page 11 LB 983 NightOWL II Operating Manual Safety Instructions  L’instrument est fourni avec une fiches à 3 broches dont une prise de terre. C’est une prescription de sécurité. Il est nécessaire que cette fiche puisse être branchèe sur prise reliée à la terre. Dans le cas contraire, il vous faut alors en avertir un électricien afin d’installer une telle prise.
Page 12 LB 983 NightOWL II Operating Manual Safety Instructions  Respecter toutes les prescriptions légales concernant la manipula- tion des déchets biologiques, des réactifs et des prélèvements- échantillons de patients. L’utilisateur assume la responsabilité exclusive de l’utilisation  des réactifs.  Transporter l’appareil uniquement dans son emballage d’origine.
Page 13: Sicherheitshinweise
Transportsicherungen vor dem Einschalten entfernen.  Die Geräte dürfen nur für den vorgesehenen Zweck eingesetzt werden.  Berthold Technologies übernimmt keinerlei Gewährleistung, auch für Schäden gegenüber Dritten, die durch unsachgemäße Handhabung der Geräte hervorgerufen werden.  Die Geräte dürfen nur innerhalb geschossenen Räumen betrieben werden.
Page 14  Achtung: Die interene Batterie kann bei flaschem Einbau explodieren! Die interne Batterie darf nur mit dem gleichen oder einem empfohlenen Ersatzmodell von einer von Berthold Technologies autorisierten Person ausgetauscht werden. Verbrauchte Batterien müssen entsprechend den Herstelleranweisungen entsorgt werden. ...
Page 15 LB 983 NightOWL II Operating Manual Safety Instructions  Elektrostatische Aufladungen (z.B. durch Teppichböden) müssen beim Öffnen des Gerätes verhindert werden, da Entladungen am Gerät zur Beschädigung elektronischer Teile führen können.  System muss immer ausreichend Reagenzienhersteller empfohlenen Lösungen gespült werden. ...
Page 17: Warranty And Customer Service
This warranty does not apply to any third party product involved in the application. Berthold Technologies reserves the right to refuse to accept the return of any product that has been used with radioactive or (micro)biological substances, or any other material that may be deemed hazardous to employees of Berthold Technologies.
Page 18: Customer Service
This form should then be transmitted to your Berthold Technologies representative or to Technical Support at Berthold Technologies, where it will receive early attention.
Page 19: Quick Start
LB 983 NightOWL II Operating Manual Quick Start Quick Start These quick start instructions provide a quick access to the program. You are guided step by step through the major program functions. The page numbers in the headers refer to the indi- vidual sections in the manual that provide more detailed information about the respective func- tions.
Page 20: Installation
LB 983 NightOWL II Operating Manual Quick Start Installation 4.1.1 Safety Instructions Whenever you are working with UV transilluminators you have to wear safety equipment (safety glass, face protecting mask) to rule out any hazards due to ultraviolet rays. The transilluminator is designed for operation in the NightOWL and should not be operated outside the device.
Page 21: Unpacking
LB 983 NightOWL II Operating Manual Quick Start 4.1.3 Unpacking  Unpack the boxes and make sure you have everything. In case you discover any sign of damage on the instruments or instrument parts, notify the shipping agent and/or the distributor immediate- Remove the transport safety devices with care! ...
Page 22 LB 983 NightOWL II Operating Manual Quick Start Ethernet camera (NC100e, NC320e) Due to the system design, forced ventilation is used for this camera type. A fan installed into the ventilation cap pumps air through the camera by means of a hose bellows; the air exits again on the opposite side.
Page 23: Installing The Base Plate
NightOWL to socket (1) on the rear panel via a RJ-45-network Träge Netzsicherung Slow Blow Fuse BERTHOLD TECHNOLOGIES 75323 Bad Wildbad cable with the network interface card of the PC. It does Made in Germany not matter if you are using a regular patch cable or a crossed patch cable (cross-over cable), since the cable type is detected automatically and the polarity is adapted.
Page 24: Connecting To The Pc
LB 983 NightOWL II Operating Manual Quick Start 4.1.7 Connecting to the PC Ethernet Version (NC100e, NC320eID 40508-10; 40508-20) Darkbox Control L A N Camera Control An Ethernet as well as an USB-connection connection is required to control the NightOWL II with NC100e or NC320e camera and to transfer data.
Page 25: Power On
If after inserting / power on of the NightOWL II a ‚USB -> Serial Converter‘ is identified instead of ‚Berthold Technologies NightOWL‘, the NightOWL II has not yet been programmed for opera- tion with indiGO. In this case, please contact the Berthold customer service to have the device re-programmed accordingly.
Page 26: Software Installation
LB 983 NightOWL II Operating Manual Quick Start 4.1.9 Software Installation The indiGO software is included on the enclosed USB stick or CD. Keep this media in a secure place. You will need it again for program updates or new installation. For the operation of the NightOWL the indiGO software version 2.0.1 or higher is necessary.
Page 27 LB 983 NightOWL II Operating Manual Quick Start Depending on the hardware you use, a default selection of system filters might be available and can be installed through the setup. If you want to install a set of empty default filter slider and wheel tick the box “Install default filters”. The default filters are empty filter sliders without any filters.
Page 28: Instrument Controller First Time Initialization Setup
You can check the instrument ident-number by starting „Scan Devices“. If the calibration for your device was stored on the Instrument by Berthold Technologies before it was delivered you can download the stored calibration parameters from the instruments flash memory now by con- firming „Yes“.
Page 29 LB 983 NightOWL II Operating Manual Quick Start This step of the Instrument Controller First Time Initialization Setup allows you to import further created backups of the Instrument configuration, users, filters and calibration files. If there is a backup available, chose the command that suits you best. Select to continue.
Page 30: First Time Initialization Setup
LB 983 NightOWL II Operating Manual Quick Start Select to continue. On this screen you can chose when you want to start the controller. With regular installations it is recommended to select the first option (Start controller with indiGO). The indiGO controller takes care of the hardware control and performs the measurements even when indiGO itself is not running.
Page 31 LB 983 NightOWL II Operating Manual Quick Start The first time initialization setup will start. If the PC running indiGO is conneted to the NightOWL directly through USB choose “Server” to install the instrument controller as well as the evaluation part. In case of a network connection to a NightOWL, select “Client”...
Page 32 LB 983 NightOWL II Operating Manual Quick Start Old databases created with indiGO Version 1.x are not compatible. The data created with older indiGO versions or WinLight can be imported into the new indiGO database using the new indiGO software (see 6.9.3). Click to continue.
Page 33 LB 983 NightOWL II Operating Manual Quick Start To set up indiGO to automatically logon to the database using a default user account, tick the box <Set a default user account>. By selecting <Next> you will be asked to set up a user name and a passwort. Please note that the created account will have administrator rights.
Page 34: User Management
LB 983 NightOWL II Operating Manual Quick Start 4.1.12 User Management IndiGO supports an integrated user management. The User Management can be enabled or disabled via the system administrator. Refer to the <User Management> <Advanced> settings to enable or disable the user management. Only Admins can access the user management.
Page 35: Installing Fluorescence Filters
LB 983 NightOWL II Operating Manual Quick Start 4.1.13 Installing Fluorescence Filters The NightOWL II is equipped with automatic filter changers for the excitation and emission path. The excitation filter is located on a filter slider, which is accessible after opening the cover on the front panel of the NightOWL II.
Page 36: Filter Management
LB 983 NightOWL II Operating Manual Quick Start An illustration of the filter wheel is given below (filter wheel shown from above). The positions of the filters are numbered and identified by a respective number printed on the filter wheel. For luminescence and photographic measurements one filter position (position 4)must be open.
Page 37: Filter Inventory
LB 983 NightOWL II Operating Manual Quick Start 4.1.15 Filter Inventory The filter inventory is used to describe the available filters. indiGO governs two filter inventory lists. One is used for the excitation filters, the other for the emission filters. The inventory lists can be reached via <Excitation Inventory>...
Page 38: Filter Manager
LB 983 NightOWL II Operating Manual Quick Start 4.1.16 Filter Manager Once a filter was added to the filter inventory list the filter can be placed on an excitation filter slider or an emission filter wheel. The filter manager can be reached via <Excitation Inventory> <Filter Manager> for the excita- tions filters or via <Emission Inventory>...
Page 39: First Acquisitions
LB 983 NightOWL II Operating Manual Quick Start 4.2 First Acquisitions 4.2.1 Quick Start Glossary Projects, Applications and Identities: All parameters necessary to run a measurement are defined in an application. Individual probes, called identities, can be associated to a project. The project is connected to an applica- tion.
Page 40: Defining The First Application
LB 983 NightOWL II Operating Manual Quick Start 4.2.3 Defining the First Application In indiGO different measurement methods (e.g. luminescence measurement, fluorescence measurement) are defined by diverse applications. For an application the measurement mode, the exposure time, the measurement sequence and the filter settings, if available can be as- signed.
Page 41 LB 983 NightOWL II Operating Manual Quick Start Select to define the measurement workflow. Four different kinds of meaurements can be performed. Please note that depending on the sys- tem configuration some of the selections might not be possible (e.g. due to a missing fluores- cence module).
Page 42 LB 983 NightOWL II Operating Manual Quick Start The Post Processor page offers additional image processing features which can be performed after the exposure to increase the image quality.  Cosmic suppression is an imaging filter to get rid of small high intensity pixels caused by cosmic ray during the exposure.
Page 43 LB 983 NightOWL II Operating Manual Quick Start To insert a photographic exposure to the list of measurements select <Add>, <Photo>. The default parameters (100ms exposure time, 10% illumination intensity) can be used for most applications. Disable all Post Processors in the corresponding section. The “close iris” feature will improve image quality (depth of focus).
Page 44 LB 983 NightOWL II Operating Manual Quick Start You can either select an already existing format using the load button or define a new sample format. The following parameters are required:  Sample Size: The sample size refers to the image size (width) in mm. To make sure that the sample will be measured completely it is recommended to define the image size a little bit larger than the actual size of the sample ...
Page 45 LB 983 NightOWL II Operating Manual Quick Start On this page you can set up the “Region of Interest” settings as well as the “Multi Sample” pa- rameters. By using the “Region of Interest” a section of the CCD chip can be defined to get faster framerates.
Page 46: Defining A Controller Template
LB 983 NightOWL II Operating Manual Quick Start 4.2.4 Defining a Controller Template When creating a controller template the workflow is about the same than with a measurement sequence. The difference is that no measurements are performed. The controller templates are mainly used to control the LED panels in order to simulate environmental parameters (day night cycle).
Page 47 LB 983 NightOWL II Operating Manual Quick Start Adjust the required intensities and click on to accept the parameters. The control com- mand will show up in the work flow list. When all commands are set close the dialog box and save the template by clicking on Please note that while a control sequence is running no measurements can be performed.
Page 48 LB 983 NightOWL II Operating Manual Quick Start  Heater Sets the target temperature of the temperature controlled measuring table (Option).  Relay Out Controls the power socket inside the dark room (Relay 1). Relay 2 is not used yet. ...
Page 49: Defining The First Project
LB 983 NightOWL II Operating Manual Quick Start 4.2.5 Defining the First Project To define a new project refer to <Project & Analysis>, <Available Projects>. Select <New Project > from the <Available Projects > context menu. Projects are used to define non measurement-specific parameters, like user access rights, scale settings, etc.
Page 50 LB 983 NightOWL II Operating Manual Quick Start A prefix, the current year and month, the 3 digit sample ID counter as well as a suffix can be specified if required. The resulting sample ID name can be seen under “Result”. The box “Num- ber of Identities to Create”...
Page 51 LB 983 NightOWL II Operating Manual Quick Start On the last page you can assign users to the project. The projects creator automatically re- ceives ownership for the project. Other users can be added to the project. To create new users refer to the indiGO user manager.
Page 52: The First Acquisition
LB 983 NightOWL II Operating Manual Quick Start 4.2.6 The First Acquisition The system can carry out measurements on demand or scheduled using a time table. In the first step it is a lot easier to create your first acquisitions on demand. Further details about how to use the scheduler can be found at the end of the chapter.
Page 53 LB 983 NightOWL II Operating Manual Quick Start To start the actual measurement sequence select . Upon completion of the measure- ment, the data is automatically stored and displayed on the screen. The measurement consists of a photographic image in grayscales and the luminescence image combined in pseudo color.
Page 54: Image Sequences
LB 983 NightOWL II Operating Manual Quick Start 4.2.7 Image Sequences In addition to single measurements indigo also offers automatic sequence measurements. These kind of measurements allows automatically triggered measurements in a predefined schedule. To creat a sequence measurement proceed as follows. To start a sequence refer to <Acquisition &...
Page 55 LB 983 NightOWL II Operating Manual Quick Start In this dialog box the Starting time for the first measurement or control sequence can be adjust- ed. In the section “Execution calendar” the total number of measurements / sequences as well as the interval time can be selected.
Page 56: Image Processing And Evaluation Functions
LB 983 NightOWL II Operating Manual Quick Start 4.3 Image Processing and Evaluation Functions 4.3.1 Load Images During the first acquisition images have been created and stored in the project folder. To re-open the previously taken images refer to the project folder first and select the appropriate identity.
Page 57: The Graphical User Interface
LB 983 NightOWL II Operating Manual Quick Start 4.3.2 The Graphical User Interface After an acquisition or the opening of an identity the measurement and the photographic image are displayed. By default the photographic image is overlaid by the measurement or signal im- age using pseudocolors.
Page 58 LB 983 NightOWL II Operating Manual Quick Start The scaling method can be changed via the context menu which can be in- voked by clicking on the arrow next to the icon. The following options for the scaling are available: ...
Page 59 LB 983 NightOWL II Operating Manual Quick Start The command has to be confirmed and can be parameterized using the dialog shown below. The following options are available: <Apply Image and Contrast settings> will apply the image and contrast settings from the cur- rent image to all other measurements in the project.
Page 60: Line Plot
LB 983 NightOWL II Operating Manual Quick Start 4.3.3 Line Plot For further analysis of the measurements it might be helpful to create a 1D-line plot of the measured data. This function is recommendet to evaluate the measuring data. To use this function select An additional window is opened where the line profile created from a given lead is displayed.
Page 61: Surface Plot
LB 983 NightOWL II Operating Manual Quick Start 4.3.4 Surface Plot To get a 3D surface plot select <Surface> from the <Report> menu.The surface can be used for 3D visualization of the measured data. To create a 3D surface plot from the photographic image only please refer to associated command.
Page 62: Peaksearch
LB 983 NightOWL II Operating Manual Quick Start 4.3.5 Peaksearch Once the image has been loaded and the color view has been set up, you can start to analyse the evaluation. In the first step the desired areas has to be marked; now indiGO can calculate the intensity val- ues.
Page 63: Define Areas Manually
LB 983 NightOWL II Operating Manual Quick Start  None No discrimination method is being used. Every spot which is above the given lower threshold will be treated as peak. Advanced - Remove Areas Inside Areas If this option is set areas which are located inside other areas will be declined. Maximum Areas This parameter defines the maximum amount of peaks.
Page 64 LB 983 NightOWL II Operating Manual Quick Start To define a freehand shape circle the desired area with several clicks on the left mouse button or hold it down completely (pencil mode). A double-click will end the procedure. The painted shape will be connected automatically and the freehand shape will be closed by a frame.
Page 65 LB 983 NightOWL II Operating Manual Quick Start To export areas the <Export> command from the “Overlay Properties” can be used. To import areas the <Import> command from the “Overlay Properties” can be used.
Page 66: Creating A Report
LB 983 NightOWL II Operating Manual Quick Start 4.4 Creating a Report 4.4.1 Creating a Rich-Text-Report indiGO offers the possibility to create and display measurement reports. The output can be adapted to the user's requirements. Using the <Rich-Text-Report> command in the <Report> menu will start the Rich-Text-Report- Creation-Wizard.
Page 67 LB 983 NightOWL II Operating Manual Quick Start...
Page 68: Excel Export
LB 983 NightOWL II Operating Manual Quick Start 4.4.2 Excel Export indiGO provides a very comfortable report function, the possibility to create Excel exports. In an Excel export, a complete measurement report, including all images, analysis data and in addi- tion, diagrams containing a graphical view of the measurement results can be created.
Page 69 LB 983 NightOWL II Operating Manual Quick Start  Measured Data Different units for the measured data can be selected.  Excel Export Options The report can be opened to be printed or stored into a file for further usage. The spreadsheet will contain one to three worksheets depending on the selected options: ...
Page 70: Using The Scheduler
LB 983 NightOWL II Operating Manual Quick Start 4.4.3 Using the Scheduler Control sequences like sunset and sunrise or measurements for selected identities can be con- trolled via a scheduler. You can think of the scheduler as a simple list of time controlled events. To start an acquisition, refer to <Acquisition &...
Page 71 LB 983 NightOWL II Operating Manual Quick Start When selecting <Schedule Controller Template> a dialog box pops up to select the desired control sequence. Chose to confirm the selected template and continue. On the next page the <Start Execution> time can be selected. In the sample above, the first measurement will be executed on Wednesday the 22th of September at 15:15:00.
Page 72 LB 983 NightOWL II Operating Manual Quick Start Note: If you open the selected project and the scheduled identity, the measured image data will be displayed automatically, too.
Page 73: System Description
LB 983 NightOWL II Operating Manual System Description System Description Use and Function Design of the Acquisition Device Acquisition Chamber 5.3.1 Light-tight Door with Door Button 5.3.2 Illumination of the Acquisition Chamber (Dark Box) 5.3.3 Connector Field 5.3.4 Camera and Lens 5.3.5 Camera Transport Unit 5.3.6 Camera Lift 5.3.7 Filter Wheel...
Page 74: Use And Function
System Description 5.1 Use and Function NightOWL II LB 983 is a powerful Imaging System for luminescence and fluorescence meas- urements. It is used to acquire and evaluate images in the macro and micro area in a large in- tensity range.
Page 75: Design Of The Acquisition Device
LB 983 NightOWL II Operating Manual System Description 5.2 Design of the Acquisition Device The acquisition device includes the hardware and electronics required for image acquisition. It comprises the acquisition chamber (dark box) including the camera lift with camera, lens and filter wheel. The elec- tronics and excitation light source for fluorescence measurements are accommodated in the upper part of the device.
Page 76 LB 983 NightOWL II Operating Manual System Description Front side Light-tight door with handle to close the acquisition chamber Door for loading and unloading the filter slider for fluorescence excitation Operating status indicator Inside the acquisition chamber Inductive sensor detecting the door position Bottom plate (removable) to position the acquisition objects 4 LED's illuminating the acquisition chamber Camera lift with transport unit, camera, lens and filter wheel...
Page 77: Acquisition Chamber
LB 983 NightOWL II Operating Manual System Description 5.3 Acquisition Chamber 5.3.1 Light-tight Door with Door Button To close the acquisition chamber light-tight, the door frame is provided with an elastic sealing and a handle which, when turned by 180°, will pull the door into the frame and squeeze it against the sealing.
Page 78: Camera And Lens
System Description 5.3.4 Camera and Lens NightOWL II LB 983 can operate with different camera systems. The following camera is sup- plied as standard: NightOWL Cam NC 100U, hermetically vacuum encapsulated and cooled down by a Peltier element to -70°C (the operating temperature is reached after about 10 minutes).
Page 79: Camera Lift
LB 983 NightOWL II Operating Manual System Description The camera transport unit includes a device to prevent collisions with the bottom or ceiling of the camera box, i.e. a micro switch stops the lift movement (audible click), when the camera transport unit collides with the sample.
Page 80: Filter Slider
LB 983 NightOWL II Operating Manual System Description 5.3.8 Filter Slider The filter slider can accommodate 4 excitation filters with a diameter of 25 mm (or 1 inch). Use the IndiGO software function [Start an acquisition] menu to open the filter slider menu. Click the button to eject the filter slider.
Page 81 LB 983 NightOWL II Operating Manual System Description In the application definition you can select the excitation and emission filter and the lamp energy for fluorescence measurements. For best results we recommend a lamp energy between 80 and 100%. Available filters can be selected depending on which filter wheel / slider configura- tion you have selected in the <Excitation>...
Page 82: Bottom Plate
LB 983 NightOWL II Operating Manual System Description 5.3.9 Bottom Plate The bottom plate helps you to position the acquisition objects. It includes two bolt heads on the front panel to help you position and fix the samples or sample masks accurately. In addition, there are 2 drilled holes for accurate positioning of sample holders.
Page 83: Top Part Of Acquisition Device
LB 983 NightOWL II Operating Manual System Description 5.3.10 Top Part of Acquisition Device The upper part of the instrument contains the chute of the camera lift, the fluorescence light source (option) and the complete electronics. Chute of camera lift Ventilation slits for cooling Electronics and...
Page 84: Taking Off The Protective Cover
LB 983 NightOWL II Operating Manual System Description 5.3.12 Taking Off the Protective Cover Before taking off the protective cover, you have to  Pull the power cord before you open the device! In the bottom area of the rear side of the protective cover there are 2 holes behind which there are white push-buttons.
Page 85 LB 983 NightOWL II Operating Manual System Description  Switch on the NightOWL and wait until the camera has moved into the upper position. Switch off the NightOWL after the the position is reached.  Replug the power and USB cable at the camera, place the microscope in the darkbox and mount the camera on the microscope C-mount adapter ...
Page 87: Indigo Software Documentation
LB 983 NightOWL II Operating Manual indiGO Software Documentation indiGO Software Documentation Graphical User Interface Application Toolbar Identity Context Identities and Measurements Contrast Slider 6.5.1 Photo Properties Quick Menu 6.5.2 Photo Properties Dialog Data Slider 6.6.1 Data Properties Quick Menu 6.6.2 Data Properties Dialog Toolbar IndiGO Menu...
Page 88: Graphical User Interface
LB 983 NightOWL II Operating Manual indiGO Software Documentation 6.1 Graphical User Interface The indiGO graphical user interface is clearly structured and offers a variety of setup options. Measurements are automatically displayed as overlay. 6.2 Application Toolbar On the toolbar you find the following commands: ...
Page 89: Identity Context
LB 983 NightOWL II Operating Manual indiGO Software Documentation  Device State Open a window to display the current state of the NightOWL.  Scheduler Open the editor for the Scheduler  Report Create a report for the current active project ...
Page 90 LB 983 NightOWL II Operating Manual indiGO Software Documentation  Apply to Identity With this command the current image and contrast settings, as well as the overlay proper- ties of the annotation and the manual layers can be applied to all measurements of the cur- rent identity.
Page 91: Identities And Measurements
LB 983 NightOWL II Operating Manual indiGO Software Documentation 6.4 Identities and Measurements  Top Row Currently opened identities  Center Row List of all measurements belonging to the current selected identity  Bottom Row Measurement or data toolbar 6.5 Contrast Slider With the contrast slider on the left side of the image the brightness of the photographic display is defined.
Page 92: Photo Properties Quick Menu
LB 983 NightOWL II Operating Manual indiGO Software Documentation 6.5.1 Photo Properties Quick Menu  Reload Settings Reload the last stored settings fort he scaling and the alignment.  Scale n Scale the contrast of the photo using the selected algorithm. ...
Page 93 LB 983 NightOWL II Operating Manual indiGO Software Documentation Reference: true name photo displayed link. example: 20100301005800.photo.000, where 201003 is the date the photo was taken. In our example: 03. March 2010. 005800 is the recording time. In our example: 12:58:00 AM. By following the link, the indiGO image viewer will be opened and the photo will be displayed.
Page 94: Data Slider
LB 983 NightOWL II Operating Manual indiGO Software Documentation 6.6 Data Slider On the right margin of the screen you see the intensity scale of the measurement, to- gether with the associated slider. With these two sliders you can set the scaling of the pseudocolor signal image and the lower threshold of the color view.
Page 95 LB 983 NightOWL II Operating Manual indiGO Software Documentation For further analysis a LUT and a surface is supported. In addition the overly intensity can be set through <Overlay level>.
Page 96 LB 983 NightOWL II Operating Manual indiGO Software Documentation  Filter The Filter page can be used to change or setup the filter properties used to render the over- lay. The following options are available:  False Color Table Activates the system default false color table ...
Page 97: Toolbar
LB 983 NightOWL II Operating Manual indiGO Software Documentation 6.7 Toolbar  Decrease the zoom Used to decrement the zoom  Increase the zoom Used to increment the zoom  Current zoom Used to setup the zoom manually, displays current zoom factor ...
Page 98 LB 983 NightOWL II Operating Manual indiGO Software Documentation  Manually (Menu) Create manual areas  Peak Search (Menu) Starts the peak search  Peak Search (Command)
Page 99 LB 983 NightOWL II Operating Manual indiGO Software Documentation  Overlay Properties (Dialog) The <Overlay Properties> dialog can be opened through the <Annotaion>, <Manual- ly> or <Peak Search> commands  Line Plot Starts the Line Plot Tool  Report (Menu) ...
Page 100: Indigo Menu
LB 983 NightOWL II Operating Manual indiGO Software Documentation 6.8 IndiGO Menu 6.8.1 New Project To create a new project, start the wizard. For further details please refer to section 4.2.4. Controller Template To create a new controller template, start the wizard. For further details please refer to section 4.2.4.
Page 101: Print Report
LB 983 NightOWL II Operating Manual indiGO Software Documentation 6.8.6 Print Report indiGO offers the possibility to create and display measurement reports. The output can be adapted to the user's requirements. For further details please refer to chapter 4.4. 6.8.7 Logoff This command will change the current user account.
Page 102: Tools Menu
LB 983 NightOWL II Operating Manual indiGO Software Documentation 6.9 Tools Menu 6.9.1 Backup Projects IndiGO can create backups of the database or selected projects. Backups are created using the backup smart wizard. The wizard will guide you through the steps necessary to successfully create a backup. When selecteing the Backup first define which projects have to be backed up.
Page 103: Import Winlight Data
LB 983 NightOWL II Operating Manual indiGO Software Documentation After launching the wizard just select the project file (.proj) of the indiGO 1.x project to import. When the standard database location was used, the files can be found using this file structure: C:\Database1.0\User\Username\Projects\Projectname\Projectname.proj The “Destination Project”...
Page 104: Service Menu
LB 983 NightOWL II Operating Manual indiGO Software Documentation 6.10 Service Menu 6.10.1 Filter Manager This command will start the indiGO filter manager. For further details please refer to section 4.1.13 6.10.2 User Manager This command will start the indiGO user manager. For further details please refer to section 4.1.11.
Page 105: Controller Service
LB 983 NightOWL II Operating Manual indiGO Software Documentation 6.10.3 Controller Service The <Controller Service> command will start the Instrument Controller Setup. The Instrument Controller Setup is used to setup your NightShade or NightOwl device and to perform all system calibration tasks. 6.10.4 Device State The <Device State>...
Page 106: Quick Check
LB 983 NightOWL II Operating Manual indiGO Software Documentation The button <Z-Axis to Transport Position> moves the Z-drive to a transport position in combi- nation with a specific foam piece to ensure a safe transport of the NightOWL. This function even works when the instrument is not equipped with a camera.
Page 107: Controller Version
LB 983 NightOWL II Operating Manual indiGO Software Documentation Each highlevel quickcheck command will start a smart wizard which will guide you through the neccessary parameter setup steps to perform the quickcheck. 6.10.7 Controller Version The command will display the current controller and instrument version information. Please note: Watch the owl while moving the mouse.
Page 108 LB 983 NightOWL II Operating Manual indiGO Software Documentation Overlay Access to overlay colour options IndiGO Access to setup configuration wizards and connectivity settings necessary for update and sup- port mail access. Setup Database Connection Using this wizard the connection type can be changed. This might be required when the com- puter was moved away from the system or in case the database was changed from the local disk to a network drive.
Page 109 LB 983 NightOWL II Operating Manual indiGO Software Documentation Setup Default User With this wizard a default user can be assigned who logs in automatically after launching indiGO. The user management can be disabled completely or a default user who logs on automatically at program start can be assigned.
Page 110: View Menu
LB 983 NightOWL II Operating Manual indiGO Software Documentation 6.11 View Menu 6.11.1 Toolbar This command shows or hides the toolbar. This option is not persistent and the toolbar will be shown on the next start of indiGO. 6.11.2 Statusbar This command shows or hides the status bar.
Page 111: Trouble Shooting
LB 983 NightOWL II Operating Manual Trouble Shooting Trouble Shooting Graphical User Interface Application Toolbar Identity Context Identities and Measurements Contrast Slider 6.5.1 Photo Properties Quick Menu 6.5.2 Photo Properties Dialog Data Slider 6.6.1 Data Properties Quick Menu 6.6.2 Data Properties Dialog Toolbar IndiGO Menu 6.8.1 New...
Page 112: Hardware
LB 983 NightOWL II Operating Manual Trouble Shooting 7.1 Hardware 7.1.1 What to do if collisions occur? If a collision of the camera lift with the sample or the sample carrier has occurred, a micro switch stops the lift movement immediately. In this case, the IndiGO software shows a message that a collision has occurred and provides information to remedy the error.
Page 113: Camera Lift Hits The Ceiling Of The Acquisition Chamber
LB 983 NightOWL II Operating Manual Trouble Shooting Set the home position as described above (step 3). 7.1.4 Camera Lift Hits the Ceiling of the Acquisition Chamber Take the bottom plate out of the acquisition chamber. You may hold it on both slants on the side.
Page 114: Software
LB 983 NightOWL II Operating Manual Trouble Shooting 7.2 Software 7.2.1 Surface plot not visible When running the surface plot from the Report menu a box with an error message (D3D device error) pops up instead of the surface plot. This indicates that the installed graphics card or the corresponding driver does not support Microsoft Windows DirectX 9 features.
Page 115: Cleaning And Maintenance
LB 983 NightOWL II Operating Manual Cleaning and Maintenance Cleaning and Maintenance Camera and Lens 8.1.1 Cleaning the Camera Box/Removing the Bottom Plate 8.1.2 Cleaning the Camera 8.1.3 Cleaning the Lens Darkbox 8.2.1 Replacing the Air Filter 8.2.2 Replacing the Fuse 8.2.3 Setting the Door Sensor...
Page 116: Camera And Lens
LB 983 NightOWL II Operating Manual Cleaning and Maintenance 8.1 Camera and Lens 8.1.1 Cleaning the Camera Box/Removing the Bottom Plate The camera box itself should only be cleaned using a mild detergent and a lint-free tissue or a vacuum cleaner. To this end, the bottom plate which is held by two mag- nets can be removed.
Page 117: Cleaning The Lens
LB 983 NightOWL II Operating Manual Cleaning and Maintenance 8.1.3 Cleaning the Lens The lens can only be cleaned if no filter wheel is installed. It should only be cleaned using pres- surized air spray or cleaning agents available in photo shops! Move the camera transport unit to the working height, i.e.
Page 118: Darkbox
LB 983 NightOWL II Operating Manual Cleaning and Maintenance 8.2 Darkbox 8.2.1 Replacing the Air Filter The air filter should either be cleaned or replaced, depending on the level of contamination. Remove the black ventilator cover on the rear of the instrument and clean the filter (blow it out or wash it) or replace it.
Page 119: Technical Data
LB 983 NightOWL II Operating Manual Technical Data Technical Data Camera Peltier/air-cooled slow scan CCD camera C-Mount adapter Height can be changed by the software to change imaging size and focusing . Resolution 1024 x 1024 Pixel x 13,6µ (back illuminated NC-100 Camera) 2184 x 1472 Pixel x 6.8µ...
Page 121: Appendix
LB 983 NightOWL II Operating Manual Appendix Appendix 10.1 Accessories 10.1.1 Transilluminator General Safety Instructions Make sure to use a grounded mains outlet when connecting the transilluminator to the power supply! Connect the transilluminator only to the power supply specified on the type label of the device! Never leave the transilluminator powered up without any supervision! Pull the power cord before carrying out maintenance and repair work and make sure that all parts of the device you may possibly come into contact have cooled down to room temperature!
Page 122 LB 983 NightOWL II Operating Manual Appendix a) UV Transilluminators If you are working with UV transilluminators, it is extremely important that optimum protection of skin and eyes will be guaranteed. You should wear UV safety glasses or a UV face protection mask.
Page 123 LB 983 NightOWL II Operating Manual Appendix 10.1.2 Macro Measurement Table The macro measurement table is used for measurements in the close-up range if you are work- ing with very small samples. Due to the elevated position of the table, the camera can move closer to the object.
Page 124 LB 983 NightOWL II Operating Manual Appendix 10.1.4 Line Light The line light is used to illuminate reflecting fluorescence samples. Reflections on the sample are ruled out by the fact that the lights are positioned at the side. The angle of illumination can be set by adjusting the two lights.
Page 125 LB 983 NightOWL II Operating Manual Appendix 10.1.6 3D-Orthogonal Mirror Table The 3D Orthogonal Mirror Table is used to measure an animal from 3 side simultaneously. THis is done throgh a specific mirror setup. The Mirror assembly is located on a special carrier. This carrier is placed on a base plate which is positioning the carrier under the camera.
Page 126 LB 983 NightOWL II Operating Manual Appendix 10.1.7 Test Light Source The Test Light Source is used to calibrate and test the System.. It contains a low light source which will glow for about 15 min. without external supply. To charge the unit, use the included Mains adapter.
Page 127: Gas Anesthesia Unit
LB 983 NightOWL II Operating Manual Appendix 10.2 Gas Anesthesia Unit The gas anesthesia unit is designed to anesthesize rodents while measuring them in the NightOWL. A version for 3 and 5 animals is available. The unit consists of the following mod- ules: Sample table with gas masks.
Page 128: Installing The Gas Anesthesia Unit
LB 983 NightOWL II Operating Manual Appendix 10.3 Installing the Gas Anesthesia Unit The modules for the gas anesthesia unit are preassembled. Only a few tubes have to be con- nected. The function of the unit is described in a short functional description: The incoming pressured air is regulated at the flow meter and mixed with the oxygen after hav- ing passed through the flow meter.
Page 129 LB 983 NightOWL II Operating Manual Appendix 10.3.1 Simplified Anesthetic Protocol for Rats and Mice Before starting Check if vaporizer is full. Fill up if necessary (see instructions for vaporizer rack). Check weight of the activated carbon filters. Each filter whose weight has increase more than grams...
Page 130 LB 983 NightOWL II Operating Manual Appendix Awaking / end using controls Turn vaporizer off (turn controller to 0FF). Turn off oxygen flow meter. Move animal(s) from NightOWL to waking box. Turn off mask(s) exhauster. Check all floodgates of stainless steel beam holder and NightOWL are closed. Turn off compressor (wait for the engine to stop if it running), then open bleed screw.
Page 131: Nightowl Ii Shipping Instructions
LB 983 NightOWL II Operating Manual Appendix 10.4 NightOWL II Shipping instructions The NightOWL II must be shipped with the camera removed and the foam piece laid on the bot- tom plate fixed with the camera lift as shown below: Proceed as follows: ...
Page 132 LB 983 NightOWL II Operating Manual Appendix Noise Killer hood Foam block ...
Page 133: Customer Reply Form
LB 983 NightOWL II Operating Manual Appendix 10.5 Customer Reply Form Send Customer Reply Form to: Berthold Technologies GmbH & Co.KG Technical Support Calmbacher Str. 22 75323 Bad Wildbad Germany Phone: +49 7081 177 114 Fax: +49 7081 177 301 Email: service@berthold.com...
Page 134 LB 983 NightOWL II Operating Manual Appendix Customer Reply Form Date: Customer no.: Name: Company: Department: Address: Address: Phone: Fax: Email: Instrument: ID no.: Serial no.: Embedded software version: Instrument driver software version: Accessory instruments: PC Software: PC software version: Windows version: Computer type: CPU type:...
Page 135: Confirmation On Decontamination
Appendix 10.6 Confirmation on Decontamination If you return an instrument to BERTHOLD TECHNOLOGIES for servicing purposes which is not properly decontaminated, there will be a health risk for BERTHOLD TECHNOLOGIES employ- ees. We therefore need your confirmation that the instrument was decontaminated and cleaned properly before shipping.
Page 136 LB 983 NightOWL II Operating Manual Appendix 10.7 Index 3D Orthogonal Mirror Table first time initialization Flatfield Correction acquisition Admin fuse Anesthesia induction Gain Anesthetic Protocol gas anesthesia unit Application goose neck light guide Area Guest Peaksearch Import Background Correction indiGO 1.x Data Backup Projects WinLight Data...

BERTHOLD TECHNOLOGIES NightOWL II LB 983 Operating Manual

Table of Contents

4 Quick Start

5 System Description

6 Indigo Software Documentation

7 Trouble Shooting

8 Cleaning and Maintenance

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