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Plethysmometer User Guide SKU 37240 - Rev. 1.0 - Nov. 2023 ugobasile.com YOUR COMPANION IN DISCOVERY SINCE 1963...
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Service and adjustments should be carried out by qualified personnel, authorized by Ugo Basile organization. Any adjustment, maintenance and repair of the powered instrument should be avoided as much as...
Product features and general information What’s in the box General 3.1. Principle of operation ................9 Intrument description 4.1. The Electronic Unit 37240-001 ............10 4.2. The water cell ..................10 Installation 5.1. Unpacking & preliminary check ............11 5.2.
1. Product features and general information This original device by Ugo Basile was designed to measure paw volume changes (swelling) in rodents. With almost 4,000 peer-reviewed citations, Ugo Basile Plethysmometer is the gold standard for paw swelling tests. Main features include:...
& cell to drain E-AU 101 USB Pen Drive including instruction manual (with CE certi- fication) and X-Pad software 37215-303 Pedal Switch Plethysmometer kit with 25mm Rat paw tube cell -SKU: 37240-25 Q.ty Description 37240-001 Plethysmometer electronic unit 37240-210...
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Plethysmometer kit with 35mm Rat paw tube cell -SKU: 37240-35 Q.ty Description 37240-001 Plethysmometer electronic unit 37240-210 Plethysmometer cell stand 37259 Special water cell with Rat paw tube (diam. 35 mm) with water level transducer 3740-154 Water tank 7155 Set of 6 calibration probes (0.1, 0.2, 0.5, 1, 2, 4 ml)
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Available spare parts Description 7182 18 mm tube for the rat paw 7186 13 mm tube for the rat paw 7187 25 mm tube for the rat paw 7189 35 mm tube for the rat paw 7153-L Water level transducer E-BT 008 Battery type CR2032 E-FT010-1...
The Ugo Basile Plethysmometer is a volume meter, designed for accurate measurements of the rodent paw swelling. It consists of a liquid-filled cell into which the rodent paw is dipped. A transducer...
The experimental data are shown on the multifunction graphic display, in four digits, with 0.01 ml resolution. 4.2. The water cell The standard kit SKU: 37240 includes a standard water cell for Rat, diam. 18 mm, provided with an extra paw tube diam. 13 mm for measuring the mouse paw volume.
5. Installation 5.1. Unpacking & preliminary check Check the contents of the shipment for completeness, checl list to hand, and visually inspect the instrument as soon you take it out the packaging. If the instrument is damaged, immediately inform the shipping agent or carrier and notifying us by email at support@ugobasile.com Inspect the instrument for damages such as scratches, broken or loose parts.
5. Do not spray any liquid on the connectors, display, or on the electronic unit. Ugo Basile cannot in any way and form be held responsible for damage caused to things and people and warranty will be void, due to: •...
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The connection module on the front panel (Figure “Connection Panel”) encompasses the following connectors, from left to right: • Upper and lower USB ports: enable data export to a PC (via a USB pen drive), and allow for firmware upgrades. Moreover, experiment data created with the X-Pad software (see specific manual for details) can be uploaded into the device by using a USB pen.
6. Operation 6.1. Setting-Up the Plethysmometer Place the Electronic Unit and the Stand on a stable and reasonably flat bench/ table (stand, clamp and reservoir may differ from what featured in the picture). Fasten the water cell to the laboratory stand by means of the enclosed clamp.
Getting familiar with the Plethysmometer home screen is very easy (see figure “Main Menu”). Main Menu From the home page, press the “Ugo Basile logo” button on the top bar for software and memory information. Press the back button to roll back into “Main Menu” page.
In order check out your actual device firmware revision press the Ugo Basile logo icon at the home page windows and note the revision number at the right top of the screen (e.g.
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Updating Screen The Date-Time Menu allows you to adjust the date and time of the device, as shown in the below picture. Date and time setting are essential for experiment result data correct population, please set the correct date and time of Your location. Date-Time Menu ERASE DB function: The device memory can be fully erased, by tapping onto the Erase DB Icon, this...
Calibration button is explained in the chapter 6.2 6.1.4. Device setup menu The Setup Icon, accessible from the Main Menu, gives access to: • Network, for device LAN connection setup. • Auto-reading, to set-up the automatic measurement saving functions. See par. 6.3.2 for details.
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be automatically populated when in DHCP mode; in manual mode need to be manually populate and the range for the group number need to be 0 or 255. Usual value is 255.255.255.0. This number needs to be defined by your IT department, an arbitrary value can damage the functionality of other LAN connected devices.
6.2. Calibration Calibration is essential for precise measurements. Calibration is necessary in the following cases: Every time the device is powered on. • If the instrument has been not used for a long time. • After having changed the dipping solution. •...
1. Select the calibration volume from the list on the left you decide to use (similar to the paw volume you wish to measure) 2. Wait for the instrument to zeroing . Do not touch the water cell. 3. Dip the desired calibration value into the cell and leave it to the cell bottom. 4.
6.3.1. Manual measurement The manual measurement mode is shown in the “MAN” icon on the right of the selected paw, highlighted in grey. Press the “ZERO” button on the screen and then dip the animal paw into the cell, paying attention to the position of the paw in the cell. Dipping the paw at different levels for repeated measurement may result in different readings.
valid measuring value. Insert the proper value and press OK. When enabled, the measurement menu will show as follows: Measurement Menu The “AUTO” icon will show as orange, meaning that the device is ready to measure. Press the “ZERO” button on the screen. To enable the measuring phase, press the PLAY button or press the switch pedal.
6.5. Results 6.5.1. Results and Experiment menus The Plethysmometer will save measurement values (volume [ml], calibration value [mV/ml] and zero value [mV]), in addition to the descriptive data, if they have been input in the Experiment field (Treatment, Protocol, ID, Stage, Trial). See image below.
The USB Storage Window Important: data can be saved into the provided USB pen or into any other USB pen. However, if a non-Ugo Basile USB pen drive is used, make sure it is formatted as FAT32 and equal or smaller than 16GB.
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Note: if you find this number with all zero values, review the network configuration of the Plethysmometer. The login page should appear: Login Page Default password is UgoBasile (CASE SENSITIVE). Prompt the default password and press LOGIN. If succeeded, the following page will be displayed: Result Page •...
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Loading X-Pad Experiment data from a PC web browser: It is possible to populate the Experiment data of the Plethysmometer via a Windows PC, using the provided Ugo Basile X-Pad Windows application which is included into the Plethysmometer provided USB pen-drive.
6.6. TTL Connections The Plethysmometer is provided with a D-sub (DA-15 Female) TTL I/O port. This port could be used to synchronize some events with external instruments or acquisition systems. TTL Output signals are electrical isolated to guarantee galvanic isolation between the device and any other external device. TTL signals are refereed to Power Ground (pin 14 and pin 15) D-sub connector pins DB-15 Pin# Signal Name...
7. Maintenance While any service of the instrument ought to be carried out by Ugo Basile personnel or by qualified personnel authorized by UGO BASILE organization, this manual section describes normal maintenance procedures which can be carried out at your facility.
7.4. Long Inactivity The instrument does not require any particular maintenance after long inactivity, except cleaning. 7.5. Customer Support For any further information you may desire concerning the use and/or maintenance of the Plethysmometer, please do not hesitate to contact our service department (or our local distributor) either directly or via our support web form.
Plethysmometer is covered by a 12-month warranty + 12 after product registration Optional Warranty 37240-UBC12 UB Care 12 Additional hardware warranty extension 12 months for Plethysmometer (Valid for SKU 37240) 37240-UBC24 UB Care 24 Additional hardware warranty extension 24 months for Plethysmometer (Valid for SKU 37240) 37240-25-UBC12...
9. Bibliografy Selection of papers mentioning Ugo Basile Plethysmometer A. M. Ajayi, C. B. Ola, M. B. Ezeagu, P. A. Adeleke, K. A. John, M. O. Ologe, B. Ben-Azu, S. Umukoro, 2023, “Chemical characterization, anti-nociceptive and anti-inflammatory activities of Plukenetia conophora seed oil in experimental rodent models”, Journal of Ethnopharmacology...
Estimates suggest that 20% of adults suffer from pain globally. Chronic pain is the most common cause of long-term disabilities. Since 1963, Ugo Basile’s devices have increasingly acquired a leading role in the field of pain and inflammation preclinical research, becoming precious tools for researchers to achieve their experimental objectives.
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37300 - I.R. Heat-Flux Radiometer for 37215 - Analgesy-Meter Randall-Selitto Tail Flick and Plantar Test paw-pressure test 38500 - PAM Pressure Application 31300 - Orofacial Stimulation Test Measurement (for joint pain) (Fehrenbacher, Henry, Hargreaves method) 35300 - Hot/Cold Plate NG for 35350 - Thermal Place Preference (TPP screening of thermal hyperalgesia/ Test) for Mice &...
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Your Companion in Discovery since 1963 ugobasile.com more than 40,000 citations in the main bibliographic search engines. Ugo Basile SRL Società Unipersonale Via Giuseppe Di Vittorio, 2 21036 Gemonio (VA) ITALY Tel. +39 0332 744574 sales@ugobasile.com service@ugobasile.com...
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