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Summary of Contents for MyGo MINI PCR3208
- Page 1 P E R S O N A L P R E C I S I O N U S E R G U I D E...
- Page 2 WELCOME Thank you for choosing the MyGo Mini. The MyGo Mini is the world’s most compact real-time PCR instrument, with no moving parts providing silent operation and maximum reliability. With outstanding precision, it is capable of providing great results over a broad range of applications.
- Page 3 CONTENT This quick start guide will teach you what you need to know to start running your MyGo Mini. It describes everything from opening and closing the lid, to data analysis for your qPCR experiments. Contents include... S E T U P...
- Page 4 5 . U S B D R I V E 6 . U S B D R I V E EXTENSION CABLE Please keep the box the MyGo Mini came in, in case you need to protect your MyGo Mini during storage or shipment in future.
- Page 5 L I D If the instrument is The lid of the MyGo Mini connected to power the can be removed from the instrument will say “Lid on” instrument and must be and “Lid off” when you attach attached at all times when and remove the heated lid.
- Page 6 M YG O M I N I CO N N EC T I O N S If you turn your MyGo Mini upside down you will see three connections as shown below: P O W E R This is to connect your MyGo Mini to the provided power supply unit. USB Port Ethernet Port Power...
- Page 7 The display LEDs will light up multi-coloured, and then turn green if a lid is attached, or yellow if not. Your MyGo Mini is ready to run. CO N N EC T I N G...
- Page 8 MyGo Mini Connection Modes 1. Direct network connection 2. Local area network connection 3. USB drive connection...
- Page 9 MYGO MINI LIGHTS AND SOUNDS The MyGo Mini uses display LEDs and sounds to help you understand what the instrument is doing. Here is a summary of these display LEDs and sounds: D I S P L AY L E D s...
- Page 10 I N S TA L L I N G YO U R M YG O M I N I S O F T WA R E Your MyGo USB drive contains software for Windows, Mac OS X and Linux operating systems. Please open the software file matching your chosen operating system.
- Page 11 CONFIGURING YOUR MYGO MINI With your MyGo Mini software open and your MyGo Mini connected to the network please connect to your MyGo Mini Instrument. Please select Configuration...
- Page 12 Please select MyGo Mini To add your new MyGo Mini select the Add b utton. You will now be presented with a list of available instruments.
- Page 13 Double-click on the instrument you wish to connect to, or pr ess Select with the instrument selected. The instrument will now be added to the list of Registered Instruments for use in the software.
- Page 14 7 . P C S E T T I N G S L I D G E T S WA R M The MyGo Mini lid can get warm during operation, this is completely normal. D O N OT L E AV E T H E L I D O F F If the lid is off dust may fall into the wells and affect the performance of your instrument.
- Page 15 R E M OV E A L L B U B B L E S Bubbles can cause optical artefacts as shown in the graph below. Ensure that no bubbles are present in reaction volumes. Artefact Cycle K E E P YO U R L A B C L E A N Please keep your work space clean including all lab equipment like surfaces, pipettes, and tube racks.
- Page 16 EXPERIMENT This section will teach you everything you need to know to get started with MyGo Mini experiments. You will learn how to create, save, open and close experiments. You will also learn how to set up an experiment including thermal profile, sample information and optical settings.
- Page 17 E X P E R I M E N T S U M M A RY Once you have created a new experiment the E xperiment Summary w ill be displayed as shown below: By default the experiment name will be “N ew Experiment”...
- Page 18 OPEN AN EXPERIMENT By selecting O pen y ou can open an experiment as shown below: F I L E S O F T Y P E The Files of Type drop down menu gives you the option to open an experiment normally or as a template that can be used for a new run.
- Page 19 E X P E R I M E N T F I L E An experiment file will contain all the data and analyses but can not be re-run. To open as an Experiment File s elect the first option shown on the opposite page.
- Page 20 SAVING AN EXPERIMENT Once an experiment has completed it can either be automatically saved to a pre-set location on your computer or manually saved by you. The experiment must be saved before the software is closed to ensure that data is not lost. AU TO M AT I C SAV I N G To automatically save your...
- Page 21 M A N UA L SAV I N G Once your experiment has finished you can save your experiment manually by selecting S ave or S ave As. I f your experiment is unsaved, you will be prompted to choose a location to save your experiment.
- Page 22 SETTING UP A PROFILE In this section we will cover Hold Times, Programs and setting up a temperature profile. H O L D T I M E S During thermal cycling protocols hold times should be chosen to allow for the following: thermal equilibration of reaction volumes;...
- Page 23 A thermal profile is required to perform an experiment. Select the profile tab as shown below: P R O G R A M S To add a program to your experiment click A dd i n the P rograms p ane. Programs can be deleted by selecting them in the list then clicking D elete.
- Page 24 T E M P E R AT U R E P R O F I L E Once programs have been added to the experiment they will appear in the T emperature Profile p ane in the order they are present in the P rograms window as shown below: P R O G R A M S E T T I N G S...
- Page 25 SAMPLES You will now learn how to set up samples and targets which can be defined as follows: SA M P L E S TA R G E T S A description of the specimen A molecular target e.g. “x174”, being analysed e.g.
- Page 26 A D D I N G SA M P L E S A N D TA R G E T S Samples and targets can be added and removed from the experiment by selecting + and - in the Samples and Targets pane.
- Page 27 DY E CO M PAT I B I L I T Y The MyGo Mini comes pre-calibrated for 11 different fluorescent labels, shown in the table below. The Auto Dye File Generation software analysis module enables you to calibrate your MyGo Mini for additional fluorophores if required. Factory Calibrated Dyes...
- Page 28 SAV I N G A N D O P E N I N G A SA M P L E S E T U P If you wish to save or open sample and target information setup you can select Save As... or Open, respectively. Sample and target setups can be saved in the following formats: Editable .csv files.
- Page 29 RUN SETTINGS The MyGo Mini software provides default optical settings which do not need to be changed for most applications. To view them, select the R un Settings tab. C YC L I N G ACQ U I S I T I O N S...
- Page 30 Here, if necessary, you can tailor optical settings to your assay’s characteristics. Integration Time controls the exposure time, in seconds, of the optical sensor inside the MyGo instrument. Brighter reagents require shorter integration times than dimmer reagents. Longer integration times will provide improved signal to noise with dim fluorescent reporters.
- Page 31 Here is where you can review the current dyes present in your experiment. You have the following options when working with dyes in your MyGo Mini software: O P E N Open dye calibrations from a file to be added to this experiment.
- Page 32 STARTING A RUN FROM THE SOFTWARE To start a run from the MyGo Mini software select S tart Run. Y ou will then be presented with the auto save options (unless you have chosen not to be prompted) and then be asked to choose an instrument from the list of R egistered Instruments.
- Page 33 LOADING TUBES INTO YOUR MYGO MINI In order to ensure that the heated lid is balanced, please make sure that the mount contains a tube in each of positions 1, 5, 9, and 13.
- Page 34 M E LT I N G Automatically determine the melting temperature of your amplicons using A utomatic Tm Calling. R U N F U S I O N Combine data from multiple runs on your MyGo Mini for analysis together.
- Page 35 SELECT ANALYSIS TYPE You can add an analysis type by selecting the “+” button in the bottom left of the software window. Analysis types are separat ed int o Automatic and Manual sections. Automatic analysis types use advanced automated data processing to provide accurate results with minimal user intervention.
- Page 36 AU TO M AT I C Q UA N T I F I C AT I O N P O S I T I V E / N EG AT I V E C A L L I N G The first step in amplification analysis is to determine which targets have produced a positive amplification curve, and which amplifications have produced a negative amplification curve.
- Page 37 I N T E N S I T Y Intensity is a measure of the size of the exponential phase of the amplification. Note that this may be smaller for amplifications with a negative baseline drift. High P E R F O R M A N C E Performance is a measure related to the observed efficiency of amplification at later cycles.
- Page 38 S E T T I N G S The software uses a combination of Quality (Q), Performance (P), and Intensity (I) thresholds to determine if an amplification is positive (green zone) or negative (red zone). All values (Q, P and I) must be above the relevant threshold for an amplification to be called positive.
- Page 39 A RT E FAC T F I LT E R I N G Biochemical and physical factors can cause fluorescence levels to change during a run. These are often observed during early cycles. Filtering out these artefacts can improve the accuracy of amplification analysis.
- Page 40 N O R M A L I SAT I O N O P T I O N S Here you can choose the way your data is normalised before display in the amplification graph. By default Full normalisation is applied to enable the discrimination of subtle differences in amplification.
- Page 41 R E S U LT S A S TA B L E The results of amplification analysis are presented in the Results as Table view. Q UA N T I F I E R S If you have assigned standards with different known quantities to your experiment, for example a dilution series, you will be able to see your reaction efficiency by selecting Quantifiers.
- Page 42 M U LT I P L E X I N G DY E PA I R S The MyGo Mini supports multiplexing with the following dye pairs Dye Pair Notes FAM, HEX Cost effective probe synthesis FAM, VIC e.g. Applied Biosystems...
- Page 43 DY E C A L I B R AT I O N R U N You will need to perform a real time PCR run in order to generate a dye file. Every well of your MyGo Mini should contain the same reagents. These reagents should produce a single PCR product, and a strong signal for the dye that you are calibrating.
- Page 44 AU TO DY E C A L I B R AT I O N Select the Auto Dye Calibration option in the Select Analysis Type window. The Auto Dye Calibration analysis module will analyse changes in fluorescence during the dye calibration run to create a new dye file.
- Page 45 E X P O RT C A L I B R AT I O N Select the Dye Data tab and you can choose to export the calibration to a file, or, use it in your current experiment. Select the Use Wells drop down menu and you will see the list of samples present in the experiment.
- Page 46 G E N OT Y P I N G AU TO M AT I C E N D P O I N T G E N OT Y P I N G TaqMan genotyping experiments can be analysed automatically using A utomatic Endpoint Genotyping. Genotypes of samples are determined by the ratio of endpoint fluorescence between two TaqMan probes.
- Page 47 D I F F E R E N C E , N O R M A L I Z E D A N D M E LT Once opened the analysis module will automatically determine optimised parameters and generate a set of D ifference, Normalized a nd M elt g raphs.
- Page 48 M E LT I N G AU TO M AT I C T M C A L L I N G Below an Automatic Tm Calling module has been added to the experiment and the software has automatically identified and characterised a melting peak.
- Page 49 AU TO S E T T I N G S The user has the option to change the target if the experiment contains more than one target. The signal-to-noise ratio setting determines how big a peak should be before it is called. Increasing this value will increase specificity of peak detection.
- Page 50 R U N F U S I O N Run Fusion enables you to combine data from multiple runs from your MyGo Mini together for analysis. To fuse multiple experiments together hold down cmd on a Mac or Ctrl on a PC and select the files you wish to fuse from the open dialog window as shown below.
- Page 51 If you are fusing experiments from multiple file locations you must select the first experiment and then select the “+” from the top left of the open file dialogue window as shown below. Now hold cmd/Ctrl and select the next experiment. Select Open to fuse your experiments together.
- Page 52 EXPORT When you have finished analysing your data in the MyGo software, you can export the results in a variety of ways, from raw data to user defined custom reports. This section will take you through the steps you need in order to do this.
- Page 53 To export data click the Export button, shown on the previous page. Data can be exported in the following formats: This is an editable data file that can be opened in many spreadsheet applications. his file format is suitable for archiving, printing and presentations, but cannot be edited.
- Page 54 GRAPH EXPORT From panes showing results as graphs, such as amplification and melt curve data can be exported by selecting the button from the below example. The user can export information as bitmap images (.PNG), Scalable Vector Graphics (.SVG), Character-Separated Variable (.CSV), or Portable Document Format (.PDF).
- Page 55 R E P O RT G E N E R AT I O N Customisable reports can be generated from your experiment by selecting Experiment Report under the Experiment tab. This provides you with the ability to choose which parts of the data to include in a report.
- Page 56 MAINTENANCE This section will help you take care of your MyGo Mini. It covers the following areas: 1 . C L E A N I N G 3 . ENVIRONMENTAL CO N D I T I O N S 2. D I S P O SA B L E S...
- Page 57 D ECO N TA M I N AT I O N If your instrument needs decontaminating please follow the instructions contained in the MyGo Decontamination Guide . Please note that for health and safety reasons you must print and complete a physical copy of the decontamination form, and include this with any instrument or lid that is returned.
- Page 58 ENVIRONMENTAL CONDITIONS YO U R W O R K S PAC E Your MyGo Mini instrument should be placed on a flat , dry, surface that is not subject to draughts. Do not install your MyGo Mini instrument directly in the flow of air from an air conditioner or fan.
- Page 59 Pressure 0 to 3000 MAMSL 70 to 106Kpa D I SA S S E M B LY Please note that your MyGo Mini contains no user- serviceable components inside. Any disassembly of your MyGo Mini instrument will void all warranties.
- Page 60 TROUBLESHOOTING This section will help you troubleshoot your MyGo Mini if you think something is wrong. Here are some frequently asked questions, and the answers. How do I create a template? All experiments can be used as a template. Click “Open”...
- Page 61 Generally, modern instruments do not require the use of passive fluorescent references. The MyGo instruments do not require the use of ROX as a passive reference. Is it normal for the lid of my Yes.
- Page 62 ERROR MESSAGES Your MyGo Mini will let you know when something is wrong by displaying an error. Most errors are reported as messages in the Status Bar in the MyGo software. Some errors are reported by the instrument display LEDs.
- Page 63 The lid must not be removed Lid was removed during until the run finishes. See “MyGo run - instrument will abort. Mini Lights and Sounds” for a description of how to confirm the instrument has finished .
- Page 64 Instrument timed out Please ensure the lid is fitted waiting for heated lid. correctly, and the ambient temperature is in the required range of +15ºC to +32ºC . Could not start run Please use the log button - instrument has to view the detailed message, encountered a and send this, plus a copy...
- Page 65 LEDs flashing red. If your instrument display LEDs are flashing red, please contact technical support. LOG FILES In the unlikely event that your MyGo Mini has a problem, you can produce a log file to send to technical support. For software faults, click the Configuration button >...
- Page 66 NOTES...
- Page 67 If you are contacting us about a suspected fault we would also recommend you send us the ppf experiment file from the run where the problem was observed. All trademarks are the property of their respective owners. Design and specifications are subject to change without notice.
- Page 68 MyGo Mini www.mygopcr.com Version 005 (EN)
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