MyGo Mini User Manual
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MyGo Mini User Manual

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MyGo
Mini
P E R S O N A L P R E C I S I O N
U S E R G U I D E

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Summary of Contents for MyGo Mini

  • Page 1 MyGo Mini P E R S O N A L P R E C I S I O N U S E R G U I D E...
  • Page 2 This quick start guide will teach you what you need Thank you for choosing the MyGo Mini. to know to start running your MyGo Mini. It describes The MyGo Mini is the world’s most compact everything from opening and closing the lid, to data real-time PCR instrument, with no moving parts analysis for your qPCR experiments.
  • Page 3 6 . U S B D R I V E EXTENSION CABLE Lugs Please keep the box the MyGo Mini came in, in case you need to protect your MyGo Mini during storage or shipment in future. Grooves...
  • Page 4 P O W E R is attached, or yellow if not. Your MyGo Mini is ready This is to connect your MyGo Mini to the provided power supply unit. to run. C O N N E C T I N G...
  • Page 5 MYGO MINI LIGHTS AND SOUNDS The MyGo Mini uses display LEDs and sounds to MyGo Mini Connection Modes help you understand what the instrument is doing. Here is a summary of these display LEDs and sounds: D I S P L AY L E D s...
  • Page 6 CONFIGURING YOUR MYGO MINI M YG O M I N I S O F T WA R E With your MyGo Mini software open and your MyGo Mini connected to the network please connect Your MyGo USB drive contains software for Windows, to your MyGo Mini Instrument.
  • Page 7 Please select MyGo Mini To add your new MyGo Mini select the Add button. You will now be presented with a list of available instruments. Double-click on the instrument you wish to connect to, or press Select with the instrument selected.
  • Page 8 L I D G E T S WA R M K E E P YO U R L A B C L E A N The MyGo Mini lid can get warm during operation, this is completely normal. Please keep your work space clean including all lab equipment like surfaces, pipettes, and tube racks.

  • Page 9: Create A New Experiment

    E xperiment Summary w ill be displayed as shown below: This section will teach you everything you need to know to get started with MyGo Mini experiments. You will learn how to create, save, open and close experiments. You will also learn how to set up an experiment including thermal profile, sample information and optical settings.
  • Page 10 OPEN AN EXPERIMENT E X P E R I M E N T F I L E An experiment file will contain By selecting O pen y ou can open an experiment all the data and analyses but as shown below: can not be re-run.
  • Page 11 SAVING AN EXPERIMENT M A N UA L S AV I N G Once your experiment has finished you can save your experiment Once an experiment has completed it can either be manually by selecting Save or Save As. If your experiment is unsaved, you will be prompted to choose a location to save your automatically saved to a pre-set location on your experiment.

  • Page 12: Setting Up A Profile

    SETTING UP A PROFILE A thermal profile is required to perform an experiment. In this section we will cover Hold Times, Programs and setting up a temperature profile. Select the profile tab as shown below: H O L D T I M E S During thermal cycling protocols hold times should be chosen to allow for the following: thermal equilibration of reaction volumes;...
  • Page 13 SAMPLES T E M P E R AT U R E P RO F I L E Once programs have been added to the experiment they will You will now learn how to set up samples and targets appear in the Temperature Profile p ane in the order they are present in the Programs window as shown below: which can be defined as follows: S A M P L E S...
  • Page 14 Samples and Targets pane. emission maximum between 510nm and 560nm. A list of the available dyes on the MyGo Mini can be viewed below: The order of samples and targets can be altered by clicking the Up and Down arrows in the Samples and Targets pane.

  • Page 15: Run Settings

    S AV I N G A N D O P E N I N G A S A M P L E S E T U P If you wish to save or open sample and target information setup The MyGo Mini software provides you can select Save As... or Open, respectively.
  • Page 16 Here is where you can review the current dyes present your assay’s characteristics. in your experiment. You have the following options when working with dyes in your MyGo Mini software: Integration Time controls the exposure time, in seconds, of the optical sensor inside the MyGo instrument. Brighter reagents...
  • Page 17 LOADING TUBES INTO THE SOFTWARE YOUR MYGO MINI To start a run from the MyGo Mini software select In order to ensure that the heated lid is balanced, please S tart Run. Y ou will then be presented with the auto...
  • Page 18 Automatic High Resolution Melt. M E LT I N G Automatically determine the melting temperature of your amplicons using Automatic Tm Calling. RU N F U S I O N Combine data from multiple runs on your MyGo Mini for analysis together.
  • Page 19 SELECT ANALYSIS TYPE AU TO M AT I C Q UA N T I F I C AT I O N You can add an analysis type by selecting the “+” button P O S I T I V E / N E G AT I V E C A L L I N G in the bottom left of the software window.
  • Page 20 S E T T I N G S A RT E FAC T F I LT E R I N G The software uses a combination of Quality (Q), Performance Biochemical and physical factors can cause fluorescence levels (P), and Intensity (I) thresholds to determine if an amplification to change during a run.
  • Page 21 N O R M A L I S AT I O N O P T I O N S R E S U LT S A S TA B L E Here you can choose the way your data is normalised before The results of amplification analysis are presented in the display in the amplification graph.
  • Page 22 G E N OT Y P I N G D I F F E R E N C E , N O R M A L I Z E D A N D M E LT Once opened the analysis module will automatically determine AU TO M AT I C E N D P O I N T G E N OT Y P I N G optimised parameters and generate a set of Difference, Normalized and Melt graphs.
  • Page 23 M E LT I N G AU TO S E T T I N G S The user has the option AU TO M AT I C T M C A L L I N G to change the target if the experiment contains Below an Automatic Tm Calling module has been added to the more than one target.
  • Page 24 Run Fusion enables you to combine data from multiple runs from your MyGo Mini together for analysis. To fuse multiple experiments together hold down cmd on a Mac or Ctrl on a PC and select the files you wish to fuse from the open dialog window as shown below.

  • Page 25: Data Export

    When you have finished analysing your data in the MyGo software, you can export the results in a variety of ways, from raw data to user defined custom This is an editable data file that can be opened in many spreadsheet applications.
  • Page 26 GRAPH EXPORT R E P O RT G E N E R AT I O N Customisable reports can be generated from your experiment by selecting Experiment Report under the Experiment tab. This From panes showing results as graphs, such as provides you with the ability to choose which parts of the data to include in a report.

  • Page 27: Maintenance

    MAINTENANCE CLEANING DAY-TO - DAY This section will help you take care of your MyGo Mini. For day-to-day cleaning wipe the external surface of It covers the following areas: your instrument with a damp, soft, lint-free cloth. Then 1 . C L E A N I N G 3 .

  • Page 28: Environmental Conditions

    Any disassembly of your MyGo Mini instrument will void all warranties. Your MyGo Mini instrument should be placed on a flat , dry, surface that is not subject to draughts. Do not install your MyGo Mini instrument directly in the flow of air from an air conditioner or fan.

  • Page 29: Troubleshooting

    Auto Quant relies drop down before rising on correct qualitative calling. This section will help you troubleshoot your MyGo Mini in Auto Quant? Check that appropriate if you think something is wrong. thresholds are set so that positive amplifications are called positive.

  • Page 30: Error Messages

    Your MyGo Mini will let you know when something is and all cables are attached correctly and securely. If the error still wrong by displaying an error.

  • Page 31: Log Files

    In the unlikely event that your MyGo Mini has a and lights and sounds confirm problem, you can produce a log file to send to technical it is fitted.
  • Page 32 NOTES If you are contacting us about a suspected fault we would also recommend you send us the ppf experiment file from the run where the problem was observed. All trademarks are the property of their respective owners. Design and specifications are subject to change without notice.
  • Page 33 MyGo Mini www.mygopcr.com Version 004...

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