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DNS AS-410M Operation Manual

Auto slide preparation system
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AUTO SLIDE PREPARATION SYSTEM
Operation Manual
Chapter 1.........................................Overview
Chapter 2.................................
Chapter 3.........................Preoperational Setup
Chapter 4................... Operational Procedures
Chapter 5................ Errors and Troubleshooting
Chapter 6...........Routine and Daily Maintenance
To use this product properly, please read this manual before use.
！
Please keep this manual close at hand for quick reference.
AS-410M Auto Slide Preparation System
AS-410M
Installation
IVD
Ver. １.4.24
1

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Summary of Contents for DNS AS-410M

  • Page 1 Chapter 4……….….….. Operational Procedures Chapter 5………..….. Errors and Troubleshooting Chapter 6….….…Routine and Daily Maintenance To use this product properly, please read this manual before use. ！ Please keep this manual close at hand for quick reference. Ver. １.4.24 AS-410M Auto Slide Preparation System...

  • Page 3: Before Using This Product

    Before using this product Before using this product Thank you very much for purchasing the AS-410M Auto Slide Preparation System. ！ Warning Please ensure strict compliance with the following instructions. Failure to comply may result in injury or accident. ● Limit the use of this System only to personnel well-versed in biological sample handling.

  • Page 4: For Safe And Correct Use

    This symbol informs the User of a prohibited action. Specific details are given in writing near the symbol. This symbol requires or instructs the User to carry out an action. Specific details are written near the symbol. AS-410M Auto Slide Preparation System...
  • Page 5 Failure to comply may lead to system breakdown or Warning fire. When detecting scratches in the power cord, broken ！ wire, or poor contact, stop using the System Warning immediately and contact us for repair. AS-410M Auto Slide Preparation System...

  • Page 6: Locations And Contents Of The Alert Labels

    Locations and contents of the alert labels < System with DU-100 > ③ Extension Device ② Sectioning Unit area ⑤ Carrier tape ① Used-Blade Collection Box Unit ④ Slide Dry Chamber DU-100 (100-Slide Dry Chamber) AS-410M Auto Slide Preparation System...
  • Page 7 Locations and contents of the alert labels < System with DU-400 and BS-96 > ① Used-Blade ② Sectioning Unit ⑤ Carrier tape ③ Extension Device Collection Box Unit area ④ Slide Dry Chamber DU-400 (400-Slide Dry Chamber) BS-96 (96-Block Feeding Unit) AS-410M Auto Slide Preparation System...
  • Page 8 3-11 “Setting the dedicated microtome blades (cassette) replacement in place” and 3- 12 “Setting the carrier tape roll replacement in place” of the Operation Manual. Also, be careful not to cut yourself with the microtome blade. AS-410M Auto Slide Preparation System...
  • Page 9 ③ Extension Device Heater Be careful with the heater, which may be as hot as 50℃ or more. ④ DU-100 Dry Chamber (inside) Be careful with the surrounding area may be as hot as 50℃ or more. AS-410M Auto Slide Preparation System...
  • Page 10 (Do not put finger(s) between the carrier tape feeder and the carrier tape take-up reel). Carrier tape Caution: Be careful being pinched in the carrier tape unit. Do not put your finger between the two disks of the carrier tape unit. AS-410M Auto Slide Preparation System...
  • Page 11 Locations and contents of the alert labels ⑦ 96-Sample Feeding Unit (Sample Tray Set Unit Drawer） BS-96 Sample Feeder CAUTION:FINGER TRAP・・・・BS-96 Sample Feeder Be careful with your fingers when pushing in the drawer or opening and closing the door. AS-410M Auto Slide Preparation System...
  • Page 12 Product label: Lower right part in the back of the right door (R) Component Label (options): Upper right of back of the indicator. Right door (R) Component label Open the right door of the Main Unit Faceplate attached to the left side. AS-410M Auto Slide Preparation System...

  • Page 13: Warranty

    2. Failure, injury, or damage caused due to the replacement of detachable parts with incorrect or non- genuine parts; 3. Failure, injury, or damage caused due to repair or modification performed by anyone other than DNS’s designated department; 4. Failure or damage caused due to a fire, earthquake, flood, or any other natural disaster.

  • Page 14: Table Of Contents

    BLADE COLLECTION BOX IN PLACE 3-11 S ....66 ETTING THE DEDICATED MICROTOME BLADES CASSETTE REPLACEMENT IN PLACE 3-12 S ............67 ETTING THE CARRIER TAPE ROLL REPLACEMENT IN PLACE 3-12-1 Carrier tape replacement procedure ....................67 AS-410M Auto Slide Preparation System...
  • Page 15 4-13 I ........................142 NITIALIZATION 4-13-1 Execution of initialization ......................142 4-14 S ........................143 TAINING 4-14-1 Setting procedure .........................143 4-15 P ........................145 RINT ETTINGS 4-16 E ......................... 151 RROR 4-16-1 Viewing the error log ........................151 AS-410M Auto Slide Preparation System...
  • Page 16 6-2-4 Water drainage of the humidifier ....................218 6-2-5 Compressor check ......................... 220 6-3 M ......................221 AINTENANCE CHECK 6-3-1 Timing of Maintenance check ....................... 221 ......224 CONTACT INFORMATION FOR INQUIRIES ON OPERATION, MAINTENANCE, AND REPAIRS AS-410M Auto Slide Preparation System...

  • Page 17: Chapter 1 - Overview

    Chapter 1 - Overview - Chapter 1 - Overview - AS-410M Auto Slide Preparation System...

  • Page 18: System Overview And Configuration

    Chapter 1 - Overview - This chapter contains the following information: System configuration of the AS-410M Auto Slide Preparation System; Tissue slide preparation process; System specifications; and Components and their names. 1-1 System overview and configuration Intended Use: This System automatically mounts sections of paraffin-embedded human tissue blocks (samples) to glass slides, which is usually performed manually for the preparation of human tissue sections used for histopathological diagnosis.

  • Page 19: Tissue Slide Preparation Process

    Tissue section Slide glass 4. Stretching (extension) Heat is applied to stretch and dewrinkle the sections on the slides. 5. Drying Heat is applied to dry the sections on the slides. AS-410M Auto Slide Preparation System...

  • Page 20: Additional Processes

    ● The barcode reader may fail to read the barcodes which are too faint, covered with paraffin, or stained. ● The sample identification function allows printing of the read barcode and associated data. AS-410M Auto Slide Preparation System...

  • Page 21: Block Surface Temperature Detection Function (Option)

    1-3-3 Block surface temperature detection function (option) The sensor automatically measures the surface temperature of the sample embedded block on the sample holder before sectioning. The sample is sectioned under the set temperature or lower. Non-contact surface temperature sensor AS-410M Auto Slide Preparation System...

  • Page 22: Step-Cut Function (Interval Mode)

    1-3-5 Two Sections on One Slide sample making function (option) This function mounts two sliced tissue sections on one (1) slide from the same paraffin block. ① ① ② ② Slide Two tissue sections on Slicer area one slide Serial section slicing AS-410M Auto Slide Preparation System...

  • Page 23: Blade Mark Check Function (Option)

    1-3-7 External PC Connection Expansion Package (option) This option reads printed barcode on the sample cassette automatically set at AS-410M. This function can operate automatically of the printing and section slice information from saved condition data from the PC; the function can operate from a conditional data inputted by this Software along with the set sample tray number in this System.
  • Page 24 Chapter 1 - Overview - ④ Slide printing AS-410M receives the registered data of each sample from LK-400 PC, then mode starts printing (sample number, name, QR code, etc.) onto the slide automatically. ⑤ Barcode reference check AS-410M Sample identification function checks the reference of the QR code printed on the slide and the sample (cassette) barcode information.

  • Page 25: System Specifications

    • Slides manufactured by Matsunami Glass Ind., Ltd.: Non-coated, MAS-GP type A coat, and FRONTIER coat Supported slide size • Slides manufactured by Muto Pure Chemicals Co., Ltd.: Non-coated, New Silane II, and New Silane III AS-410M Auto Slide Preparation System...
  • Page 26 Slide collection box transfer arm UP/DOWN drive chamber Option Slide collection box transfer arm TILT drive (DU-400 unit) Upper-shelf slide collection box TILT drive Lower-shelf slide collection box TILT drive Heater (600 W) Temperature control range: Room temperature to 55°C AS-410M Auto Slide Preparation System...
  • Page 27 Setting temperature range: 20℃ to 55℃ Refrigerant: R134a AC100/110/120/220/230/240V single-phase 50/60Hz 1.5KVA×2 system Power source Supply voltage is set at the time of shipment by the manufacturer. Working temperature range 10℃ to 28℃ (ambient temperature) AS-410M Auto Slide Preparation System...
  • Page 28 4 pcs. Waste filter (10 pcs.) A-50728 10 pcs. ×1 pack Prefilter A-50463 1 pc. ※1 Sample identification function option (BR unit) with Dot impact printer ※2 Sample identification function option (BR unit) with Thermal printer AS-410M Auto Slide Preparation System...

  • Page 29: Components And Their Names

    Power switch Cooling unit operation panel (door) Humidifying water bottle setting unit (door) System operation panel Paraffin waste collection unit (drawer) System-internal light switch Tissue slide preparation unit (door) Slide collection box setting unit (door) AS-410M Auto Slide Preparation System...
  • Page 30 Power cord connector (Cooler) Cooling unit drain outlet (tubing) Air tube connector (AIR IN) Humidifying-water refill pump RC232C serial port 2 (Blade mark check) Humidifier drainage lever RC232C serial port 1 (Auto Trimming) Waste fluid tank AS-410M Auto Slide Preparation System...
  • Page 31 Chapter 1 - Overview - 27´ ４2 ４6 ４5 ２9 26´ AS-410M Auto Slide Preparation System...
  • Page 32 Sample block surface temperature ３２ ４５ Stretching plate temperature controller sensor * System with the option Blade Mark Check Unit ３３ ４６ Automatic Blade Change Unit * System with the option ３４ Used-blade collection box AS-410M Auto Slide Preparation System...

  • Page 33: System With 96-Sample Feeding Option And 400-Slide Dry Chamber Option

    ４７ 400-Slide Dry Chamber Main Unit 96-Sample Feeding option Component ４７ Sample Feeding Unit (door) Upper slide collection box (shelf) ４８ *2 boxes Lower slide collection box (shelf) ４９ *2 boxes ５０ Humidifying-water bottle holder AS-410M Auto Slide Preparation System...

  • Page 34: Chapter 2 - Installation

    Chapter 2 -Installation Chapter 2 - Installation - AS-410M Auto Slide Preparation System...

  • Page 35: Unpacking And Parts Check

    With 100-slide dry chamber option: 20 pcs. 1 pc. 1 set (50 pc./cassette) With 400-slide dry chamber option: 80 pcs. Waste fluid tank Humidifying water bottle Application medium bottle 1 pc. 1 pc. 1 pc. AS-410M Auto Slide Preparation System...
  • Page 36 *With sample identification option *With sample identification option 1 copy For Thermal printer: 1pc For Dot impact printer:1pc Operation Manual Waste filter Prefilter 1 copy 10 pcs./ packet 1 pc. (Install in the Main Unit) AS-410M Auto Slide Preparation System...

  • Page 37: Installation And Requirements

    • Places where flammable gas may be present Prohibited matter Installation or relocation of this System involves very high risks. The customer must not relocate the System by themselves. When planning to relocate the System, please contact DNS. AS-410M Auto Slide Preparation System...
  • Page 38 * In this configuration, the System weighs approx. 800 kg. * Ensure that the System’s center of gravity in the depth direction will be positioned near the center of the installation space. ５０ｍｍ ２，０３０ｍｍ Center of gravity ４５０ｍｍ ４５０ｍｍ １,６００ｍｍ ４５０ｍｍ ４５０ｍｍ ２,１８０ｍｍ AS-410M Auto Slide Preparation System...
  • Page 39 * In this configuration, the System weighs approx. 900 kg. * Ensure that the System’s center of gravity in the depth direction will be positioned near the center of the installation space. ５０ｍｍ Center of ２,０３０ｍｍ gravity ４５０ｍｍ ４５０ｍｍ １,６００ｍｍ ４５０ｍｍ ４５０ｍｍ ２,４５０ｍｍ AS-410M Auto Slide Preparation System...
  • Page 40 * In this configuration, the System weighs approx. 950 kg. * Ensure that the System’s center of gravity in the depth direction will be positioned near the center of the installation space. ５０ｍｍ Center of ２,０３０ｍｍ gravity ４５０ｍｍ ４５０ｍｍ １,６００ｍｍ ４５０ｍｍ ４５０ｍｍ ２,５８０ｍｍ AS-410M Auto Slide Preparation System...
  • Page 41 * Ensure that the System’s center of gravity in the depth direction will be positioned near the center of the installation space. ５０ｍｍ Center of 2,030 mm gravity ４５０ｍｍ ４５０ｍｍ 1,600 mm ４５０ｍｍ ４５０ｍｍ ２,８５０ｍｍ AS-410M Auto Slide Preparation System...

  • Page 42: Power Requirements And Power Cable Connection

    Power cord connector for Cooling unit (CNAC2) with current rating of 15 A ① Check the power cord plugs for correct orientation and connect them to the corresponding connectors on the back of the Main Unit. AS-410M Auto Slide Preparation System...
  • Page 43 ！ Warning ● Connect the power plugs securely into the outlets. Improperly connected plugs may heat up and start a fire. ● This System must be connected to the ground. For grounding, refer to 2-4 “Grounding.” AS-410M Auto Slide Preparation System...

  • Page 44: Grounding

    Standalone compressor (Example) ！ Warning ● Supply this System with compressed air with a pressure of 0.7 MPa. Never supply compressed air outside the specified pressure. ● Do not supply any gas other than compressed air. AS-410M Auto Slide Preparation System...

  • Page 45: Connection Of The Main Unit To The Pc For Auto Trimming

    USB2 LK-400 PC expansion LAN COM1 COM2 Auto Trimming and Blade Mark Check PC ※ Port layout maybe different depending on the model. ！ Caution ● Do not use any other cable than packaged accessory. AS-410M Auto Slide Preparation System...

  • Page 46: Chapter 3 - Preoperational Setup

    Chapter 3 -Preoperational Setup- Chapter 3 - Preoperational Setup - AS-410M Auto Slide Preparation System...

  • Page 47: Main Power Circuit Breakers

    This System has the power switch on the front. Press the power switch to illuminate the power switch lamp and activate the operation panel. Press the power switch again to turn OFF the power and the power switch lamp. Power switch AS-410M Auto Slide Preparation System...

  • Page 48: Internal Light Switch

    Internal light switch 3-4 Setting samples in place 3-4-1 Supported sizes of samples and cassettes This System supports the following sizes of paraffin-embedded tissue sample and cassettes. ● Supported sample size range ● Supported cassette sizes AS-410M Auto Slide Preparation System...

  • Page 49: Setting Samples In Place

    (tilt stage) to fail to grip samples properly, resulting in an error. Check the block top surface for any paraffin shavings, etc. Check the around and bottom of the cassette for any excess paraffin. AS-410M Auto Slide Preparation System...
  • Page 50 “1,” printed on the lower right corner of the sample tray, up to the diagonally opposite “24.” After setting the samples on the tray, open the left door (L) of the Main Unit to set the 24-sample tray inside. Left door (L) 24-sample tray *Detachable Sample position Nos. AS-410M Auto Slide Preparation System...
  • Page 51 No. 1 on the near right corner of the top tray and ends with sample No. 96 set on the far-left corner of the bottom tray. 24-sample trays Sample position No. 24 *Detachable 1 to 24 No. 1 25 to 48 No. 2 No. 49 49 to 72 73 to 96 No. 73 Sample position Nos. AS-410M Auto Slide Preparation System...
  • Page 52 No. 1 1 to 24 25 to 48 No. 25 No. 49 49 to 72 73 to 96 No. 73 ④ Then, push the sample tray set unit back in position and close the door. AS-410M Auto Slide Preparation System...

  • Page 53: Setting Slides In Place

    Hydrophilic slides are recommended for use Depending on the type of the surface coating on slides, sections may peel off during staining process. For use of slides other than the recommended products, contact DNS for consultation. <Recommended products> • Matsunami Glass Ind., Ltd.: Uncoated, MAS-GP Type A-coated, and FRONTIER-coated •...
  • Page 54 ② Check slides for correct orientation and set them in the slide cassette, so that the frosted end of each slide will face toward the transparent resin side of the cassette and the mounting side of the slide should face up in the cassette. Frosted side Sample mount side （face up） transparent resin AS-410M Auto Slide Preparation System...
  • Page 55 Each slide cassette bears a number label (①, ②, ③, or ④) on the top. Set slide cassettes ① to ④ in place from the front side of the slide cassette racks of the System. Right door (R) “FRONT” label Holes Pins Front side of System AS-410M Auto Slide Preparation System...
  • Page 56 ※ Please refer to the “4-4-2-2 Slide preparation condition settings (EXPERT MODE) for details. AS-410M Auto Slide Preparation System...

  • Page 57: Setting The Humidifying-Water Bottle In Place

    ② Insert the humidifying- water bottle fully into the humidifying-water bottle holder. < Standard System with 100-slide dry chamber option unit (DU-100) > Open the door to the humidifying-water bottle holder and insert the humidifying-water bottle. AS-410M Auto Slide Preparation System...
  • Page 58 Plug the System tube joint into the humidifying-water bottle joint. Humidifying-water bottle joint System tube joint ② When removing the humidifying-water bottle, hold the joint hose by hand and press the unlock button on the joint to disconnect the System tube. Unlock button AS-410M Auto Slide Preparation System...

  • Page 59: Setting The Application Medium Bottle In Place

    * The cap has a joint and a filter. Joint Open Close Filter ② Open the right door of the Main Unit and set the application medium bottle on the bottle holder. Right door (R) Application medium bottle holder AS-410M Auto Slide Preparation System...
  • Page 60 ④ Hold the system tube joint gently by hand when taking the application medium bottle out; Push the joint release button to release the bottle. Hold the system tube joint part by hand. Push the joint release button. AS-410M Auto Slide Preparation System...

  • Page 61: Setting The Waste Fluid Tank In Place

    Make sure to check the laying pipe properly before starting the operation. Wipe with a cloth in case of the water leakage. Replace the bottle when damaged. AS-410M Auto Slide Preparation System...

  • Page 62: Setting The Slide Collection Box In Place

    Storage space 1 knob up stopper to the front Storage space 5 Slide tray ② Set each tray as the Storage space 96 edge with half-circle cut Storage space 100 outs come to the front. AS-410M Auto Slide Preparation System...
  • Page 63 FRONT label on the top of the box will come to the front side. FRONT label Set stoppers in place with their protrusions upside to prevent slides from dropping. Setting position of the slide collection box Front side of the System AS-410M Auto Slide Preparation System...
  • Page 64 Install each slide collection box ① (upper front shelf), ② (upper back shelf) ③ (lower front shelf), and ④ (lower back shelf), so that their FRONT labels will come to the front side. Number label FRONT label １ ２ ４ ３ AS-410M Auto Slide Preparation System...

  • Page 65: Setting The Used-Blade Collection Box In Place

    Used-Blade Collection Box Holding Frame in the Main Unit. Left door (L) Used-blade collection box Used-Blade Collection Box Holding Frame ！ Warning When disposing of used blades, use care to avoid cuts on the hands or fingers. AS-410M Auto Slide Preparation System...

  • Page 66: Setting The Dedicated Microtome Blades (Cassette) Replacement In Place

    ・Use dedicated-replacement microtome blades only. Failure to comply may lead to system breakdown or reduced performance. Never attempt to disassemble the replacement microtome blade cassette. AS-410M Auto Slide Preparation System...

  • Page 67: Setting The Carrier Tape Roll Replacement In Place

    Left door Right door (R) Fixing knob Carrier tape take-up reel Carrier tape feeder Guide disk ① Select Slide Preparation from the main menu and choose the tape CHANGE button. ② Touch UNLOCK DOORS button. AS-410M Auto Slide Preparation System...
  • Page 68 In the case of cellophane tape hanging over or not reaching the edges of the carrier tape, the carrier tape may fail to be fed normally, leading to such a trouble as failure of sliced sections to adhere properly to the carrier tape. AS-410M Auto Slide Preparation System...
  • Page 69 Tape and no derailment from the roller, then press OK. ⑧ Confirm enough tape is remaining. ！ Note For Carrier tape replacement, the system measures the tape remaining at initializing, therefore it can be performed when the power is off. AS-410M Auto Slide Preparation System...

  • Page 70: Routing Of The Carrier Tape

    Beware, in the case of operating with an incorrect carrier tape routing or a disengaged roller routing on the groove may cut the carrier tape during the slide preparation process. The process of the section mounting (putting) on the carrier tape may also cause a failure. AS-410M Auto Slide Preparation System...

  • Page 71: Setting The Ink Ribbon In Place

    ④ Remove the used and reeled ink ribbon and the ink ribbon core from the rollers respectively. Replace the new ink ribbon Ink ribbon cassette parts and the core to the roller AS-410M Auto Slide Preparation System...
  • Page 72 ⑧ Turn the roller to wind the ink ribbon, and remove the slack of the ribbon. Pinch the pins and turn the roller to remove the slack out the ribbon Installation of ink ribbon cassette AS-410M Auto Slide Preparation System...

  • Page 73: Setting The Suction Filter Of Paraffin Waste In Place

    ⑥ Put the pre-filter into the filter ⑦ Secure the filter holder with holder and close it. Insert the fixation screw. the suction tube into the filter holder. ※Please be careful not to spill paraffin debris when removing the filter. AS-410M Auto Slide Preparation System...

  • Page 74: Adjusting The Temperature Controllers

    (SV). The extension device and the dry chamber temperatures are factory-set to 45°C and 40°C, respectively. Follow the steps below to change the temperature settings: Present temperature Specified temperature Temperature controller Temperature controller (Dry Chamber) (Extension Device) AS-410M Auto Slide Preparation System...
  • Page 75 Extension Device: 55°C; and Dry Chamber: 50°C Temperature settings exceeding these values are NOT available. ③ After making a change to the temperature setting, press MODE button again to return to the normal display mode. MODE button AS-410M Auto Slide Preparation System...

  • Page 76: Checking The Temperature Setting Of The Colling Unit ( Built In The Main Unit )

    Be careful of your head or body with the cooler door while checking the cooler temperature. Ensure the cooler door is always closed except for checking the cooler temperature. AS-410M Auto Slide Preparation System...

  • Page 77: Setting Change Of The Cooler Temperature

    ⑧ Then, again push SET button to display ; the setting temperature is completed. ⑨After the completion of the temperature setting, push SET button and hold 5 seconds to display The screen indication will switch to the current cooling temperature. AS-410M Auto Slide Preparation System...

  • Page 78: Starting And Exiting The Auto Surface Detection Application

    3-17 Starting and exiting the Auto Surface Detection application To use the Auto Surface Detection function with the System AS-410M, the parameter setting of the Auto Surface Detection application must be setup before run. Please refer to 4-20 for parameter setting. All of the sample blocks will be processed under the same setting conditions of Auto Surface Detection application.

  • Page 79: Start-Up Of The Auto Trimming (Auto Surface Detection) Application

    ① Turn ON the power of the PC. ② The application starts; the surface detection will prepare to start. 3-17-2 Monitoring the Auto Trimming ① Index screen displays after the auto trimming completes. AS-410M Auto Slide Preparation System...

  • Page 80: Exiting The Auto Trimming (Auto Surface Detection) Application

    Follow the steps below to exit the Auto Surface Detection application: ① Click the ABORT button on the application screen. ② Click the EXIT button on the screen to exit the application. ③ Turn OFF the power of the PC. AS-410M Auto Slide Preparation System...

  • Page 81: Start-Up And Termination Of The Blade Mark Check Application

    Displays to the screen the result of the performed examination of the sections done by the main body device with Pass/Fail in color. Green background: Blade Mark Check OK Red background: Blade Mark Check NG Time and Date Result Slide ID AS-410M Auto Slide Preparation System...

  • Page 82: Termination Of The Blade Mark Check Application

    ① Click Exit(X) button at upper right on the Blade March Check application screen. ② Click Yes button on the Blade Mark Check application screen. The system completes the auto trimming application. ③ Shut down the OS of the PC to terminate. AS-410M Auto Slide Preparation System...

  • Page 83: Start-Up And Termination Of The Expansion Package Application

    The log-in screen will be displayed after the LK-400 application starts. Enter the pre-registered ID and password to operate each mode. Make sure to check that the LAN cable of the AS-410M and LK-400 PC is connected properly. Enter the administer-authorized User ID and Password to register or change a user registration or dye information;...
  • Page 84 After logging in successfully, the Main Menu screen will be displayed. For sectioning with the on-line mode, select the auto sectioning mode or manual input mode. Main Menu screen Auto sectioning mode screen (before starting) AS-410M Auto Slide Preparation System...

  • Page 85: Termination Of The Expansion Package Application

    To exit the application, press the Back button to return to the main menu, then click the Exit button, and the Yes button to terminate the application. Please shut down the OS of the personal computer. Auto sectioning mode screen (after sectioning) Main menu Exit screen Application termination screen AS-410M Auto Slide Preparation System...

  • Page 86: Safety Interlock (Door Lock)

    < System with 100-slide dry chamber unit option (DU-100) > The doors are locked upon system power-on. < System with 400-slide dry chamber option unit (DU-400) and 96-sample feeding unit option (BS-96) > The doors are locked upon system power-on. AS-410M Auto Slide Preparation System...

  • Page 87: Compressor

    The compressor will turn ON when turning the knob to the “|”side. ＜Turning OFF the compressor＞ Make sure the compressor is OFF when the AS-410M is not in use. The compressor will turn OFF when turning the knob to the “0” side.
  • Page 88 Chapter 3 -Preoperational Setup- AS-410M Auto Slide Preparation System...

  • Page 89: Chapter 4 - Operational Procedures

    Chapter 4 - Operational Procedures - Chapter 4 - Operational Procedures - AS-410M Auto Slide Preparation System...
  • Page 90 Chapter 4 -Operational Procedures- The AS-410M Auto Slide Preparation System is used for tissue slide preparation consisting of the following four steps: (1) rough-cutting (trimming: exposing the surface of a sample tissue embedded in a sample block), (2) sectioning of sample blocks, (3) transfer of a section onto a slide (attachment of tissue sections to each slide), and (4) stretching (extension) and drying of sections on slides.
  • Page 91 DU-400) BS96 (96-block feeding), SS (400-slide feeding), DU-400 (400-slide dry chamber) are option units. Printing and sectioning condition data corresponding to each sample block needs to be saved in an external PC. AS-410M Auto Slide Preparation System...

  • Page 92: Section Slide Preparation Work Flow

    Print, front blade change, auto trimming, need check or no check Execution of section slide Section slide preparation operation preparation (See 4-6) Operation during section slide preparation (See 4-7) Shutdown operation Shutdown operation (See 4-11) AS-410M Auto Slide Preparation System...

  • Page 93: Operational Parameters

    Chapter 4 - Operational Procedures - 4-2 Operational parameters The AS-410M uses [Operational Parameters] that must be selected for each run. Table 4-2 shows the outline of the [Operational Parameters] available. Table 4-2: [Operational Parameters] used for section slide preparation...
  • Page 94 ● When the cut angle is set to 60°, the cutting edge width available per block increases, ensuring a longer blade life and stabilizing the quality of slices. ● When cutting tissue slices measuring 10 mm or more, the User should set the cut angle to 60°. AS-410M Auto Slide Preparation System...

  • Page 95: Selecting The Sectioning Parameters File

    Chapter 4 - Operational Procedures - 4-3 Selecting the sectioning parameters file To operate the AS-410M, select [PARAMETER SETTING] for which the basic system operating conditions are predetermined. Select [PARAMETER SETTING] from the preexisting files. ！ Note ● Before starting section slide preparation, a parameter file must be selected. A...

  • Page 96: Editing The Sectioning Parameters

    1 to 400 be prepared per knife position. Small sized sample Select “ON” to perform sectioning based on Block Size (block) the block height measurement only. Default Current data file is updated in File No. 10 Setting AS-410M Auto Slide Preparation System...

  • Page 97: Mount Setting

    For the second section mounting, specify here the amount of -10.0 60.0 (descent) descent range by which the rollers should lower the tape held in (mm) between until the first section is transferred from the tape to a slide. AS-410M Auto Slide Preparation System...

  • Page 98: Cut Force Setting

    Decrease the tape speed deceleration rate according to the Loaded addition tape speed 0 to 99.9 (%) added percentage. Sets humidification time when the set cut force is Load humidification time 0 to 60.0 (sec) applied to the block holder. AS-410M Auto Slide Preparation System...
  • Page 99 The measured cut force is not referred to the sectioning operation. Cut Force Monitor （measured result displayed） ＜Cut Force monitor＞ The monitor displays the measured result of cut force. CUT FORCE SETTING screen ( Cut Speed & Humidify Time Offset functions disabled ) AS-410M Auto Slide Preparation System...

  • Page 100: Saving

    SAVE SECTIONING PARAMETER Screen (Save prompt) ④ Touch the file name field to open the on-screen alphanumeric keyboard and enter a user- defined file name. SAVE SECTIONING PARAMETER screen (Rename file prompt) ⑤ Touch SAVE to save the data. AS-410M Auto Slide Preparation System...

  • Page 101: Section Slide Preparation

    Touch REGISTER NAME to allow entry of up to 4 user-definable slide names (each 5 alphabetic character-long). Touch the buttons adjacent to the slide cassettes to view and set the registered slide names one by one. SLIDE GLASS SELECTION screen REGISTER NAME screen AS-410M Auto Slide Preparation System...
  • Page 102 Change tape Touch CHANGE under Tape, and the “change carrier tape” prompt will appear. Then turn OFF the power before replacing the tape. Touch CANCEL to cancel the replacement. Change carrier tape prompt AS-410M Auto Slide Preparation System...
  • Page 103 Unlock doors mounting unit, and the dry chamber. Temperature The current temperature inside the system is indicated. If the temperature is too high, sectioning will not be conducted until the temperature drops. Temperature inside the system. AS-410M Auto Slide Preparation System...

  • Page 104: Selecting The Operation Mode

    < INTERVAL mode > MAIN screen (expanded) Same setting is applied for all section preparation samples of section thickness, number of section making, slide type, number of layers, and space between layers. AS-410M Auto Slide Preparation System...
  • Page 105 < System with BS-24 > Touch the position No. buttons of the samples to be sliced. Touch ALL to select all samples. Touch RESET to deselect all samples. Slide preparation condition settings screen in STANDARD mode (Sys. with BS-24) AS-410M Auto Slide Preparation System...
  • Page 106 When the stretching time setting is updated here, the sectioning parameter values will also be updated. ⑦ Touch NEXT. AS-410M Auto Slide Preparation System...
  • Page 107 Repeat the same for Tray Nos. 2 to 4. Touch ALL to select all samples. Touch RESET to deselect all samples. Slide preparation condition settings screen in STANDARD mode (Sys. with BS-96) Tray 1 Tray 2 Tray 3 Tray 4 AS-410M Auto Slide Preparation System...
  • Page 108 For example, when cutting 3 μm-, 5 μm-, and 7 μm-sections, set 3 μm as the first condition (1st.), 5 μm as the second condition (2nd.), and 7 μm as the third condition (3rd.). AS-410M Auto Slide Preparation System...
  • Page 109 For the method of specifying stain types and sectioning thicknesses, refer to 4-15 “Staining data.” Slide preparation condition settings screen (EXPERT mode) with staining selection enabled 4-4-2-3 Slide preparation condition settings (INTERVAL Mode) AS-410M Auto Slide Preparation System...
  • Page 110 In either case, the blade change is set in the slide section parameter of each block. ④ Touch the NEXT button to set the position of the block, the numbers, etc. of the slide section condition. Refer to 4-4-2-1 “Slide preparation condition settings (STANDARD Mode)” for setting the section condition. AS-410M Auto Slide Preparation System...
  • Page 111 External PC Connection Expansion Package Option. Follow these steps for operation. ① Make sure the external PC installed with LK-400 is properly connected to AS-410M by the LAN cable (Cross). Main screen ② Turn ON the external PC, and start the LK-400 application.
  • Page 112 If the next two block numbers are empty (without two blocks), the block(s) after the two skipped spaces will not be sliced. 2 empty blocks 3 blocks set Only block No. 1 to 3 will be sliced. AS-410M Auto Slide Preparation System...
  • Page 113 If the auto-start is not checked in the AUTO mode, click the START button on the LK-400 screen and the system will start sectioning automatically. Touch the CANCEL button to cancel the operation. The screen will Preparation confirmation return to the Main screen. screen AS-410M Auto Slide Preparation System...
  • Page 114 Touch the MAIN button to return to the Main screen. Screen of slide preparation operation completion AS-410M Auto Slide Preparation System...

  • Page 115: Preoperational Self-Diagnosis

    Alternatively, touch MAIN to recheck the dry chamber or slide feeding unit. ＜Slide collection box full and slide shortage warning messages＞ No room left in the slide collection box AS-410M Auto Slide Preparation System...
  • Page 116 ● In a System with the FS option (sample identification function), an error will occur if a barcode-matching fails due to slides being incorrectly set with its frosted end upside down. Be sure to set the slides with their frosted parts upside. AS-410M Auto Slide Preparation System...

  • Page 117: Starting Slide Preparation

    < Section inspection (available only in System with the blade mark check function) > Select ON to use the section inspection; select OFF when it is not used. ③ After ensuring that everything is OK, touch START to start tissue slide making. AS-410M Auto Slide Preparation System...

  • Page 118: Screens And Operations During The Slide Preparation

    Slicing angle (pull-angle setting) Estimated end time for sectioning operation Slide preparation in progress screen (Standard mode; auto trimming function OFF) Process currently in progress Title and the section parameter number AS-410M Auto Slide Preparation System...
  • Page 119 The number of samples in the knife frame is the number of samples to be sliced per knife. When rough cutting and surface detection is not selected, the Sample/Knife setting value will appear. When rough-cutting or surface detection is selected, the Samples/Knife (R) setting value will appear. AS-410M Auto Slide Preparation System...
  • Page 120 In case of this situation, touch UNLOCK DOORS button, open the door, set the slide glass collection box again, and touch RESTART button. Slide glass take-out stopping screen in case of storage space shortage AS-410M Auto Slide Preparation System...
  • Page 121 If the remaining amount of ink ribbon is insufficient during automatic operation, the system goes into a temporary suspension state and the on-halt screen appears. Select CHANGE INK RIBBON, unlock the door, and replace the ink ribbon. Touch RESTART. The on-halt screen for ink ribbon replacement AS-410M Auto Slide Preparation System...
  • Page 122 Such caution screens do not appear during the serial sectioning operation. To cancel slide preparation, touch CANCEL. Then AS-410M brings the sample block on the sample holder back to the sample tray, returns the slide collection box to the rack, and stops the operation.

  • Page 123: Checks During The Slide Preparation

    Chapter 4 - Operational Procedures - 4-7-2 Checks during the slide preparation The AS-410M automatically stops running when an error message occurs due to any mechanical trouble detected by a sensor, a solenoid switch, etc. For corrective action, refer to Chapter 5 “Errors and Troubleshooting”...
  • Page 124 The sections attached to the tape are in the same shape as the sample block and are free from jagged edges or partial losses. c. The sections are free from wrinkles or flaws. Sectioning Unit Slice/section attached to tape Carrier tape Guide bar Microtome blade AS-410M Auto Slide Preparation System...
  • Page 125 ⑤ Guide Bar positioning If sliced sections fail to adhere on the carrier tape and the remedial actions ① to ④ above do not fix the problem, the guide bar positioning may be faulty. Contact us for advice. AS-410M Auto Slide Preparation System...
  • Page 126 ④ Lowering width, feeding speed, etc., of the carrier tape during section attachment The lowering width, feeding speed, etc., of the carrier tape are factory-set sectioning parameters. Contact us if the possible cause is the parameter settings of the carrier tape. AS-410M Auto Slide Preparation System...

  • Page 127: Emergency Stop

    Chapter 4 - Operational Procedures - 4-8 Emergency stop When it is necessary to shut down the AS-410M immediately, turn OFF the System power. ！ Warning ● Do not turn OFF the system power during operation except for cases of emergency.

  • Page 128: Shutdown Procedure

    Collect section slides and check section slide quality. Insufficient drying, (See 4-11-4.) Redo section slide preparation. Complete preparation Collect sample blocks Set the collection box back to the shelf Power ON MAIN screen Redo slide Continue drying preparation AS-410M Auto Slide Preparation System...

  • Page 129: Operation Completed Screen

    System can perform slicing. Do not use this System to slice such blocks. In the case of a very irregularly surfaced block, smooth its surface before reloading it into the System. AS-410M Auto Slide Preparation System...

  • Page 130: Switching The Modes Between The Normal Operating And The Overnight Operating

    MAIN screen when the main power is turned on at the next operation. Since the OFF TIMER is turned off once it is executed, when operating with the OFF TIMER, enable the function each time. AS-410M Auto Slide Preparation System...

  • Page 131: Saving And Deleting Operation Log Data

    ① To save log data, touch UNLOCK DOORS on the “Dry Chamber Info” screen to unlock and open the door of the touch panel. Plug the USB memory device to the USB port on the back of the touch panel. AS-410M Auto Slide Preparation System...
  • Page 132 When the file is saved normally, the “Save” prompt will close. Remove the USB memory device. To delete the log data from the System, touch and hold CLEAR (approx. 3 seconds). Slide Collect Log screen AS-410M Auto Slide Preparation System...

  • Page 133: Checking The Section Slide Quality And Collecting Section Slides

    ③ Number of slides from the same samples. ④ Slicing thickness (μm) ⑤ Month, date, hours and minutes at which slides were stored in the collection box ⑥ Slide collection box No. (System with DU-400) ⑤ ② ③ ④ ① AS-410M Auto Slide Preparation System...

  • Page 134: Maintenance Mode

    MANUAL OPERATION of the System individually. Password-protected to prevent the User from making changes to the settings. system breakdown caused by MAKER MAINTENANCE Maintenance by the manufacturer unauthorized changes to the settings invalidates manufacturer’s warranty. AS-410M Auto Slide Preparation System...

  • Page 135: Rough-Cut (Roughing) Mode

    User to check the exposed conditions of tissue samples block by block. Follow the steps below to perform a roughing using the AS-410M: 1. Use the sample transfer robot to transfer a sample block from a sample tray to the sample holder.

  • Page 136: Roughing Parameters

    3-4 and 3-8, respectively). Depending on the condition settings, the humidifying water bottle and the carrier tape must be set in place (refer to 3-6 and 3-12, respectively). In roughing operation, slides, slide collection boxes, and application medium are not necessary. AS-410M Auto Slide Preparation System...

  • Page 137: Outline Of Roughing Operation

    Specify “Slicing thickness” and “No. of cuts.” Select ON/OFF for “Humidifying” and “Tape.” (See 4-13-4.) Start roughing. (See 4-13-5) Roughing in progress Button operations during roughing (See 4-13-6) Pausing Enable attachment to carrier Step-by-step operation tape Operation completes Power OFF Shutdown procedure AS-410M Auto Slide Preparation System...

  • Page 138: Roughing Condition Settings

    Touch an appropriate numbered button or buttons to specify the position(s) of the sample(s) to be rough-cut. The selected buttons will turn bright. Roughing settings screen Roughing settings screen (System with BS-96) (System with BS-24) AS-410M Auto Slide Preparation System...
  • Page 139 1 to 96 samples Change sectioning blade shifts its position to continue roughing. The number of cutting positions of a microtome blade (available shifts) may vary depending on the pull angle setting. AS-410M Auto Slide Preparation System...

  • Page 140: Execution Of Roughing And Operation Buttons For Use During Roughing

    4-12-5 Execution of roughing and operation buttons for use during roughing Touch START on the “Roughing settings” screen. Then, the AS-410M will check the presence of a sample at the specified position and the “Start roughing” prompt will appear on the screen. Touch Start to start roughing.

  • Page 141: Roughing Completed Screen

    RESTART on the “Stopping” screen to resume the processing of the same sample. Blinks alternately in white and yellow. Roughing is stopping screen 4-12-7 Roughing completed screen When the roughing of all set sample blocks is complete, the “Roughing completed” screen appears. Roughing completed screen AS-410M Auto Slide Preparation System...

  • Page 142: Initialization

    ② Touch HOME POSITION on the “Maintenance mode” screen to open the “Initialize” prompt. MAIN screen Maintenance mode screen ③ Touch HOME POSITION to start initialization. Then, the “Initializing…” message screen will appear. Alternatively, touch BACK to cancel initialization and return to the “Maintenance mode” screen. Initialize prompt AS-410M Auto Slide Preparation System...

  • Page 143: Staining Data

    “MAIN” screen appears. “Initializing…” message screen 4-14 Staining Data The AS-410M allows the User to associate dye types with sectioning thicknesses on the “Parameter Setting” screen in the EXPERT mode. Use this screen to specify the sectioning thickness and the dye type.
  • Page 144 ON. Trim both sides No Side Trimming With Side Trimming ⑥ Touch BACK to return to the “MAINTENANCE mode” screen. Touch MAIN to return to the “MAIN” screen. AS-410M Auto Slide Preparation System...

  • Page 145: Print Settings

    Chapter 4 - Operational Procedures - 4-15 Print Settings Specify the print data items to be printed by the printer of the AS-410M. Table 4-12 “Printable Data” shows the details of the data printable by the System’s printer. Table 4-12: Printable Data...
  • Page 146 ID Nos. of Prints day and time names processed slides. entered. With dye selection enabled, the Print Settings screen line prints the names of selected Prints institution names (print data items) entered. dyes. AS-410M Auto Slide Preparation System...
  • Page 147 Enter the number of ― characters from the end characters from the end Upper line Add a line break to the Enter the characters which Lower line specified characters a line should be added Delete AS-410M Auto Slide Preparation System...
  • Page 148 Prints 2D code printed. Prints the facility name Online print setting screen （Print contents） ⑥ Touch BACK to return to the “Maintenance mode” screen. Touch MAIN to return to the “MAIN” screen. <Print content setting> AS-410M Auto Slide Preparation System...
  • Page 149 Screen to select the contents to be Print content setting screen printed ⑤ Check to confirm that the items to be printed has changed. Press back to go back to the print setting screen. Print content setting screen (end of changes) AS-410M Auto Slide Preparation System...
  • Page 150 Screen to select the contents to be Print content setting screen printed ④ Confirm that the items to be printed has changed. Then press back to go back the print setting screen. Print content setting screen (end of changes) AS-410M Auto Slide Preparation System...

  • Page 151: Error Log

    ④ When it is necessary to view an error message, touch the corresponding error code. A sub-screen will appear to show the relevant unit and part as well as the symptom. Error Log screen (overlaid with error message subscreen) AS-410M Auto Slide Preparation System...

  • Page 152: Saving The Error Log

    4-17-1 Time setting ① Touch MAINTENANCE on the “MAIN” screen. ② Touch TIME on the “Maintenance mode” screen. MAIN screen Maintenance mode screen AS-410M Auto Slide Preparation System...

  • Page 153: Language Setting

    Touch SET to update the present time based on the set time. 4-17-2 Language setting The language buttons can change the displayed language of the touch panel into Japanese and English. Language switching screen (English) Language switching screen (Japanese) Main Menu screen (English) AS-410M Auto Slide Preparation System...

  • Page 154: Manual Operation

    UPRIGHT/TILTED Printer unit LEFT/RIGHT (Y-axial); Put-on unit slide suction ON/OFF and stage PRINT UP/DOWN; and the slide-barcode reader and the sample-barcode reader scanning (reading) operation AUTO TRIMMING Diffused illumination and reflected illumination ON/OFF AS-410M Auto Slide Preparation System...

  • Page 155: Outline Of The Manual Operation Flow

    Slide transfer screen MAIN (4-19-7) BACK MOUNTING Mounting MAIN (peel and stick) screen BACK (4-19-8) DRY CHAMBER Dry chamber screen MAIN (4-19-9) BACK PRINT MAIN Print screen (4-19-10) BACK AUTO TRIMMING MAIN Auto Trimming screen (4-19-11) BACK AS-410M Auto Slide Preparation System...

  • Page 156: Sample Feeding

    SAMPLE 4: Moves the Sample Hand to sample-tray Line 4 Sample feeding operation screen (Chuck operations) SAMPLE 5: Moves the Sample Hand to sample-tray Line 5. SAMPLE 6: Moves the Sample Hand to sample-tray Line 6. AS-410M Auto Slide Preparation System...
  • Page 157 Sample feeding operation screen with Controls holder UP/DOWN Jog ③ CLAMP/UNCLAMP ON: Moves the Sample Holder Clamp downward to secure (fix) the Sample Holder. OFF: Moves the Sample Holder Clamp upward to release the Sample Holder. AS-410M Auto Slide Preparation System...
  • Page 158 The present position is displayed real-time. ② UP/DOWN UP: Moves the Sample Hand upward. DOWN: Moves the Sample Hand downward. Sample feeding operation screen (Sample Tray/Hand Operations) ③ OPEN/CLOSE OPEN: Opens the Sample Hand. CLOSE: Closes the Sample Hand. AS-410M Auto Slide Preparation System...
  • Page 159 DOWN: Moves the Sample Tray UP/DOWN to the Sample Tray Handover DOWN position. JOG: Displays the UP/DN Jog Controls on the screen. Touch and hold to move the Sample Tray upward. Touch and hold to move the Sample Tray downward. The present position is displayed real-time. AS-410M Auto Slide Preparation System...

  • Page 160: Sample Holder

    Manual operation screen Sample holder screen Sample holder screen (Sys with Trim Detection) When Auto Trimming is available, TRIM DETECT buttons appear in the “L/R (left/right)” and “U/D (up/down)” frames on the “Sample holder” screen. AS-410M Auto Slide Preparation System...
  • Page 161 Touch JOG to open the UP/DN Jog Controls on the screen. Touch and hold to move the Sample Holder upward. Touch and hold to move the Sample Holder downward. The present position is displayed real-time. AS-410M Auto Slide Preparation System...
  • Page 162 Sample Holder backward. Touch and hold to tilt the Sample Holder forward. Sample holder screen (JOG controls) Touch the X and Y values to open a numerical keypad and change the values to adjust the tilt speed. AS-410M Auto Slide Preparation System...

  • Page 163: Blade

    Item Descriptions of operation Blade Holder FWD/BWD (Y-axial) and UP/DOWN (Z-axial) Slicing Position FWD/BWD Blade Feed Air Cylinder FWD/BWD Blade Clamp LEFT/RIGHT (Y-axial) Blade Pressing CLAMP/UNCLAMP Suction Nozzle LEFT/RIGHT (Y-axial) Manual operation screen Blade screen AS-410M Auto Slide Preparation System...
  • Page 164 DOWN: Moves the blade holder to the DOWN position. ② PUSHER Blade screen （JOG controls ） PUSH: Moves the blade pusher air cylinder into the blade change position. PULL: Moves the blade puller air cylinder back into the AS-410M Auto Slide Preparation System...

  • Page 165: Carrier Tape

    Descriptions of operation Tape FWD/BWD and Take-up motor ON/OFF Tape Tension Indicates the tape tension. Tape Remaining Indicates and re-measures the remaining length of tape. Guide Bar LEFT/RIGHT and UP/DOWN Manual operation screen Tape screen AS-410M Auto Slide Preparation System...
  • Page 166 Carrier tape screen Touch and hold to shift the guide bar rightward. (JOG controls) SAVE: Registers the current position to the selected position (indicated on the Register button). AS-410M Auto Slide Preparation System...

  • Page 167: Slide Transfer

    Slide transfer screen (System with Sample identification function + 400-slide feeding unit) Operating Principle of Back Slide transfer unit Slide transfer lane Slide transfer arm Slide feed pusher Slide cassette Stretching heater unit Stretching arm Front AS-410M Auto Slide Preparation System...
  • Page 168 Available only in Systems with the 400-slide feeding unit (SS). (Stretching arm) Cassette 1: Move the slide cassette to the corresponding position. ① ② ③ ④ back front Slide transfer screen 400-slide feeding unit (Slide cassette) AS-410M Auto Slide Preparation System...

  • Page 169: Putting (Mounting) Operation

    STANDBY: Moves a fed slide to the standby position. Mounting screen (Dripping) PUSH: Moves a fed slide to the dripping position. Right Pinch Roller Left Pinch Roller Slide suction plate Operating principle of mounting unit Slide UP/DOWN air cylinder AS-410M Auto Slide Preparation System...
  • Page 170 ON (Red): Indicates that both slide suction and vacuum detection are ON. ON (Black): Indicates that slide suction is ON while vacuum detection is OFF. ⑥ TAPE SLANT DOWN: Moves the back tape inclination to DOWN position. UP: Moves the back tape inclination to UP position. AS-410M Auto Slide Preparation System...

  • Page 171: Dry Chamber

    (100-slide dry chamber unit) Slide collection box DU-400 Slide collection box shelf FWD/BWD, UP/DOWN, and TILTING (400-slide dry chamber) inside 400-slide dry chamber Manual operation screen Dry chamber screen (System with DU-100) Dry chamber screen (System with DU-400) AS-410M Auto Slide Preparation System...
  • Page 172 Touch JOG to display the UP/DN Jog controls on the screen. Touch and hold ↑ to move the slide collection box backward. Touch and hold ↓ to move the slide collection box forward. The present position is displayed real-time. Back Front Box TILT cylinder Moving directions of slide collection box AS-410M Auto Slide Preparation System...
  • Page 173 Stage 2: Moves the slide collection box Hand to the Stage 2 position. Stage 3: Moves the slide collection box Hand to the Stage 3 position. Stage 4: Moves the slide collection box Hand to the Stage 4 position. Dry chamber screen （FWD/BWD movement） AS-410M Auto Slide Preparation System...
  • Page 174 RETURN: Returns the retained box to the original position. BOX CHANGE: Retains the slide collection box bearing the number specified in the “Box change” field. Change a preexisting retained box if there are any. Operating principle of 400-slide collection box unit movement AS-410M Auto Slide Preparation System...
  • Page 175 Chapter 4 - Operational Procedures - ① Standby position ② Inserting position UP/DOWN Box TILT Cylinder Slide collection Slide collection box HAND ② ① ③ ④ Front Back AS-410M Auto Slide Preparation System...

  • Page 176: Printing

    ＜Print action＞ (LEFT/RIGHT and UP/DOWN) Test prints the specified line character and 2D code by press down : Returns the thermal head to the home position and rewind the ink ribbon. : Rewinds the ink ribbon. AS-410M Auto Slide Preparation System...
  • Page 177 Touch READ to perform the reading operation of the sample barcode reader and display the reading result (maximum of 3 characters). In case of reading failure, the following error message will be displayed. While reading Reading error AS-410M Auto Slide Preparation System...
  • Page 178 BARCODE PRINT: Moves the printer to QR code (barcode) printing position. Print screen < UP/DOWN > (LEFT/RIGHT and UP/DOWN) UP: Moves the printer to the UP position. DOWN: Moves the printer to the DOWN position. AS-410M Auto Slide Preparation System...
  • Page 179 ② Sample-barcode reader (displayed only when the reader option is enabled) Touch READ to run the Sample-barcode reader and view the scanning result (up to 32 characters). In the case of a scanning error, the following message will appear on the screen. Scanning error AS-410M Auto Slide Preparation System...

  • Page 180: Auto Trimming

    ：Turns ON the diffused illumination. ：Turns OFF the diffused illumination. < Reflected illumination > ：Turns ON the reflected illumination. ：Turns OFF the reflected illumination. Configuration of Auto Surface Detection System Diffused Illumination Front Back Camera Down Reflected illumination AS-410M Auto Slide Preparation System...

  • Page 181: Auto Trimming Setting

    Chapter 4 - Operational Procedures - 4-19 Auto trimming setting To operate the AS-410M with Auto trimming function (auto surface detection function), parameters for the auto trimming function must be set before runs. Selected samples are trimmed the under same conditions.

  • Page 182: Remote Monitoring Setting

    OFF: Select sample blocks for auto trimming and sectioning. Additional trimming (μm) Amount of additional trimming after checking the image on the Auto 0 to 999 Surface Detection Application. Blade Change Number of sectioning per blade edge. 4-20 Remote monitoring setting AS-410M Auto Slide Preparation System...
  • Page 183 Send a message at the time of an error SEND TRIGGER IN MAKING INTERVAL Send messages every set hour SAMPLE CHANGE Send messages at every completion of block sectioning. SLIDE CHANGE Send messages at every completion of slide generation. AS-410M Auto Slide Preparation System...

  • Page 184: Chapter 5 - Errors And Troubleshooting

    Chapter 5 - Errors and Troubleshooting - AS-410M Auto Slide Preparation System...
  • Page 185 This Chapter covers the troubleshooting steps for user-fixable errors and the steps for resuming operations after successful troubleshooting. ！ Warning A problem may exist in the System. Turn OFF the system power and remove the power cords from the outlets before inspecting the inside of the System. AS-410M Auto Slide Preparation System...

  • Page 186: Caution Errors (Warnings)

    A caution error can be fixed without turning OFF the system power. Just follow the warning message marked with . If, however, a system error message appears on the screen with an error code, turn OFF the system power first to check for any mechanical problems. AS-410M Auto Slide Preparation System...
  • Page 187 Touch OK on the “Check slide collection box status” screen to proceed to the next screen for entering the operating conditions. Work on and proceed to the “Slide preparation” screen. Then, start automatic operation. AS-410M Auto Slide Preparation System...
  • Page 188 Work on and proceed to the “Tissue slide making” screen. Then, start automatic operation. This caution message also appears in the case of improper slide cassette loading. In this case, turn OFF the System power to correctly set slide cassettes on the racks. AS-410M Auto Slide Preparation System...
  • Page 189 The current run can be continued without changing the tape roll but will stop when the carrier tape is used up. NO (BACK) Touch to return to Slide-Prep. Cond. Settings screen. Touch to continue the run. Carrier tape shortage screen AS-410M Auto Slide Preparation System...
  • Page 190 Touch MAIN indicated below the caution message to return to the “MAIN” screen. Then, touch SLIDE PREPARATION to open the “Preoperational setup” screen. Touch UNLOCK DOOR to open the door of the mounting unit and collect slides from the slide transfer unit. AS-410M Auto Slide Preparation System...
  • Page 191 After water is supplied, RESTART becomes enabled. Then, touch RESTART. To terminate operation, touch CANCEL. If the Humidifying water bottle is empty, refill the bottle with water. For details on the procedure, refer to 3-6 “Setting the humidifying-water bottle in place.” AS-410M Auto Slide Preparation System...

  • Page 192: Troubleshooting During Automatic Operation

    Collect samples and tissue sections (slides) carefully to avoid cuts on the hands or fingers with the slicing blade. Errors may recur. Before the slide preparation operation, we recommend using unimportant samples, such as non-tissue-containing sample blocks for test use. AS-410M Auto Slide Preparation System...
  • Page 193 (5-3 “Error Code List”) Power-ON, system check, and initialization. Initialization completes. Initialization fails to complete. Normal completion of the test run using dummy Contact us. samples (test run). Resume operation for the remaining samples (restart). AS-410M Auto Slide Preparation System...

  • Page 194: Operation Controls On The "Error" Screen

    ●The CLEAR button cannot clear some errors (air pressure error or communication error between PCBs) from the screen. In such cases, turn OFF the power. ●The MAIN button on Error screen-1 is enabled after clearing error codes. AS-410M Auto Slide Preparation System...

  • Page 195: Error Code List

    One of the doors opened when 0002 If with the BS-96, push the Sample Feeder / Part: Doors it needs to be locked. Sample Tray Set Unit firmly to the furthest back and close the door. Symptom: Door OPEN AS-410M Auto Slide Preparation System...
  • Page 196 Unit: Sample Feeding Unit Sample tray does not reach sample tray transfer arm. 1010 Part: Sample tray to the set position or store FEED/PULL position in BS-96 unit. Press the HOME POSITION button to initialize. Symptom: Positioning failure AS-410M Auto Slide Preparation System...
  • Page 197 Remove obstacles in the Sample tray <Error Message> FEED/PULL direction. Unit: Sample Feeding Unit Sample tray return motor 1030 Part: Sample tray FEED/PULL failed to complete its motion. Press the HOME POSITION button to initialize. Symptom: Stop position error AS-410M Auto Slide Preparation System...
  • Page 198 UP/DOWN (Z-axis) Part: Sectioning Clean up the sample holder or lower motor. UP/DOWN drive area with a brush etc. to remove Symptom: Limit paraffin shaves if dirty. Press the HOME POSITION button to initialize. AS-410M Auto Slide Preparation System...
  • Page 199 The blade pressing air cylinder failed confirm the normal functionality of the blade Unit: Sectioning Unit to complete operation within 5 change. 2019 Part: Blade Pressing seconds; or the two position detection sensors made ON Symptom: Operation simultaneously. error AS-410M Auto Slide Preparation System...
  • Page 200 30 seconds; or Remove the major unevenness of the 2023 Part: Height Measuring Unit the push-on distance of any of surface of the paraffin block. Symptom: Height the touch sensors is 2 mm or measurement error more. AS-410M Auto Slide Preparation System...

  • Page 201: Carrier Tape Puller Unit Errors

    Unit: Carrier Tape Puller Unit Tape length measurement Clean the tape feed roller if dirty 3009 Part: Tape Puller failed to complete within 60 with paraffin. Symptom: Tape Length Measurement seconds. Tighten the nut on the holding Error plate. AS-410M Auto Slide Preparation System...
  • Page 202 <Error Message> air cylinder failed to complete its Unit: Slide Transfer Unit 4013 motion within 5 seconds; or the Part: Transfer Arm UP/DOWN two position detection sensors Symptom: Operation Error made ON simultaneously. AS-410M Auto Slide Preparation System...
  • Page 203 60 Symptom: Initialization error seconds. <Error Message> The slide extrusion bar is not in Unit: Slide supply Unit 4027 the origin position when feeding Part: Slide extrusion bar the slide. Symptom: Origin return error AS-410M Auto Slide Preparation System...

  • Page 204: Dry Chamber Errors

    Unit: Dry Chamber 5012 chamber Z-axis motor (400- UP/DOWN drive direction. Part: Slide collection box UP/DOWN slide dry chamber). •Turn the System power ON and Symptom: Limit complete the initialization and set the slide collection box. AS-410M Auto Slide Preparation System...
  • Page 205 •Turn the System power ON and complete Symptom: Slide collection box presence/absence detection sensor the initialization and set the slide retention failure did not make ON. collection box. AS-410M Auto Slide Preparation System...

  • Page 206: Print Unit Errors

    Unit: Printer Unit 6008 operation motor failed to connector joints. Part: Printer LEFT/RIGHT operate. • Contact the customer service of DNS. Symptom: Operation error <Error Message> When the barcode read from • Check if the barcode printed on the Unit: Printer Unit...

  • Page 207: Trimming (Surface Detection) Unit Errors

    Size command response code error 7022 Size command response error 7023 Trim command response time out 7024 Trim command busy 7025 Trim command response code error 7026 Trim command response error 7027 Save command response time out AS-410M Auto Slide Preparation System...
  • Page 208 Thick command busy Symptom: 7057 Thick command response code error Communication error 7058 Thick command response error 7059 QC command response timeout 7060 QC command busy 7061 QC command response code error 7062 QC command response error AS-410M Auto Slide Preparation System...

  • Page 209: Extension Packet Errors

    Chapter 5 Errors and Troubleshooting 5-3-8 Extension Packet errors In case errors continue to occur after the AS-410M initialization and reboot of the LK-400 application, the file data may be broken or it may be a system failure. Retry the section making with a sample that has a performance record;...
  • Page 210 *1 A maximum of 96 for the System with BS-96 (96-block feeding); 24 for the System with BS-24 (24-block feeding). *2 A maximum of 400 for Systems with SS (400-slide feeding) and DU-400 (400-slide dry chamber) options; 100 for Systems other than these. AS-410M Auto Slide Preparation System...

  • Page 211: Chapter 6 - Routine And Daily Maintenance

    Chapter 6 Routine and Daily Maintenance Chapter 6 - Routine and Daily Maintenance AS-410M Auto Slide Preparation System...
  • Page 212 Chapter 6 Routine and Daily Maintenance This Chapter guides the User through the steps for performing routine maintenance and periodic inspection necessary to ensure a trouble-free long service life for AS-410M. ！ Warning ● Before system maintenance and inspection, be sure to turn OFF the system power and remove the power cords from the power outlets.

  • Page 213: Routine (Daily) Inspections

    After disposal of shavings, wipe the area with soft and dry cloth. Use a soft cloth soaked with paraffin remover, etc. to remove persistent grime. ！ Warning Before cleaning the inside of the System, empty the contents of the used- blade collection box. AS-410M Auto Slide Preparation System...

  • Page 214: Compressor Check

    Use a clean cloth soaked with water or 70-percent ethanol to clean the outside surface of the housing. Do not use organic solvents. ！ Caution Do not use thinner or any other organic solvents to clean the external surface of the housing. AS-410M Auto Slide Preparation System...

  • Page 215: Emptying And Cleaning The Waste Liquid Tank

    6-1-9 Error log check Error logs can be viewed and saved. Save the operation log data to a USB memory device as necessary. For details, refer to 4-17-2 “Saving the error log” in this Operation Manual. AS-410M Auto Slide Preparation System...

  • Page 216: Periodic (Monthly) Inspection

    Door of the cooling unit operation panel ！ Caution Make sure not to hit your head on the cooler door while checking the cooler temperature. Ensure that the cooler door is always closed except when checking the unit. AS-410M Auto Slide Preparation System...

  • Page 217: Safety Device Operation Check

    Regularly check the operation of the safety devices of the System. ！ Warning Check the operation of the safety devices approx. once a month to ensure a safe use of the AS-410M. Safety device Point to be checked The system power turns ON/OFF when the power switch is Power switch turned ON/OFF.

  • Page 218: Water Drainage Of The Humidifier

    The humidifier of the internal System has 2 liters of pure water fed from the humidifier bottle. Mold will buildup when water is left inside the humidifier. Ensure to drain the water of the humidifier once a month to avoid mold. Drainage lever Waste fluid tank Back of the Main Unit AS-410M Auto Slide Preparation System...
  • Page 219 Make sure to drain the water of the humidifier, and empty out the bottle when the System will not be used for more than a week. Mold will buildup in the humidifier and bottle if water is left inside and will cause System failure. AS-410M Auto Slide Preparation System...

  • Page 220: Compressor Check

    Unscrew the oil cap and replenish the oil (JUN-AIR SJ-27) to the upper limit line. ！ Warning When replenishing the oil, turn both power switches of the AS-410M and the compressor OFF; wait about one (1) hour. Oil cap Oil level window MAX.

  • Page 221: Maintenance Check

    6-3 Maintenance check 6-3-1 Timing of Maintenance check AS-410M is a precision instrument which requires periodic inspection and replacement of parts depending on the condition. Below are the main maintenance items and replacement parts in addition to routine inspection. Timing of inspection and parts replacement...
  • Page 222 Chapter 6 Routine and Daily Maintenance AS-410M Auto Slide Preparation System...
  • Page 223 ＭＥＭＯ AS-410M Auto Slide Preparation System...

  • Page 224: Contact Information For Inquiries On Operation, Maintenance, And Repairs

    FAX : +81-75-952-8778 http://www.dnseiki.co.jp/ E-mail: info@dnseiki.co.jp ● Authorized Representative MedNet EC-REP GmbH Borkstrasse 10, 48163 Muenster, Germany *Microsoft, Windows, Excel are trademarks or registered trademark of Microsoft Corporation in the United States and/or other countries. AS-410M Auto Slide Preparation System...

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