IBS MediaClave User Manual
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IBS MediaClave User Manual

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User Manual 3.4
Bedienungsanleitung 3.4
Mode d'emploi 3.4

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Summary of Contents for IBS MediaClave

  • Page 1 User Manual 3.4 Bedienungsanleitung 3.4 Mode d’emploi 3.4...
  • Page 2 This equipment has been Description Type, model tested and found to com- ply with the limits for a MEDIACLAVE 135020, 135025, 135030, 135035 Class A digital device, in accordance with the EC directives pursuant to both Part 15 of the FCC Rules and the...

  • Page 3: Table Of Contents

    3.2.1 Mode MEDIACLAVE ....................13 3.2.2 Mode AUTOCLAVE ....................15 3.2.3 Mode WATERBATH ....................17 3.3 Switching On and Off the MediaClave ................17 3.3.1 MediaClave start display ..................17 3.3.2 Soft key menu options explained ................18 3.3.3 LANGUAGE menu ....................19 3.3.4 PARAMETER menu ....................
  • Page 4 Table of contents 4 Instruction for use ....................25 4.1 Introduction ........................25 4.2 Safety precautions and specifications ................25 4.2.1 MEDIACLAVE and AUTOCLAVE sterilisation ............25 4.2.2 WATERBATH ......................25 4.3 Program operation ......................26 4.4 MEDIACLAVE program operation ................. 26 4.4.1 AUTOCLAVE program operation ................
  • Page 5 User Manual 3.4 7.4.2 MEDIACLAVE programs 1–13 (1–9 L of medium) ..........52 7.4.3 MEDIACLAVE program 14 (Without pressure test) ..........53 7.4.4 MEDIACLAVE program 15 (Blood Agar) ..............53 7.4.5 General default values ................... 54 7.5 Limitations and general notes ..................54 7.5.1 Overview ........................
  • Page 6 Table of contents INTEGRA Biosciences PAGE IV...

  • Page 7: Mediaclave Overview

    1.1.2 Intended use The MediaClave is a highly versatile piece of equipment. It operates as a stand-alone device used to pro- gram and control three integrated operation modes: MEDIACLAVE, AUTOCLAVE and WATERBATH. The MEDIACLAVE program is used to prepare culture media. Insertion of a stainless steel base plate and flexible PT100 temperature probe converts the MediaClave into the AUTOCLAVE.

  • Page 8: Basic Concepts

    The MediaClave offers many features to the user: • Automatic initialisation and self-test execution • Mode MEDIACLAVE – culture preparation of 1 to 9 L of agar containing medium or nutrient liquid • Mode AUTOCLAVE – autoclave for sterilisation of culture media in single containers. •...

  • Page 9: User Manual Symbols

    User Manual 3.4 The MediaClave User Manual is divided into 9 parts Chapter Name Description MediaClave overview General information Installation Installation, moving Operating controls MediaClave preparation Instructions for use MEDIACLAVE, AUTOCLAVE, WATERBATH operation Cleaning instructions Cleaning procedures Maintenance Error- and Troubleshooting, Calibration, Paper-handling...

  • Page 10: General Information

    The MediaClave User manual must be read in detail before operating the MediaClave • In the event of a situation arising where safe operation of the MediaClave cannot be guaranteed, the instrument must be switched off and locked until qualified personnel can repair it. Such a situation may include one of the following scenarios: •...

  • Page 11: Safety Regulations Concerning Service And Maintenance

    INTEGRA Biosciences AG. NOTE: MediaClave shall not be modified either as an equipment or with regard to the safety aspects. Any modification will result in exclusion of responsibility for consequential injury or damage on the part of INTEGRA Biosciences AG.
  • Page 12 MediaClave overview INTEGRA Biosciences Page 6...

  • Page 13: Installation

    Ensure all cables and tubes are positioned in a safe area to avoid people tripping over them. • Do not place anything on the MediaClave safety cover. At the end of each cycle, or if a cycle is inter- rupted and terminated, the safety cover opens automatically.

  • Page 14: Mediaclave Drawings

    MediaClave overview 2.3 MediaClave drawings 2.3.1 MediaClave vessel overview PT100 Temperature Probe (Product Sensor) Pressure Tube Grip Vessel Lid (Steam Ventilation Tube) Safety Valve Grip Vessel Lid Filling Port Dispense Port Safety Cover Lock Figure 2.1: Vessel lid INTEGRA Biosciences...

  • Page 15: Mediaclave Rear Panel

    Cooling Water Outlet Host RS232 External Printer Cooling Water Inlet Vessel Cover and External Alarm RS232 Power Supply Flow Water Outlet Cold Water Drain Figure 2.2: MediaClave rear panel Figure 2.3: Waste tube of flow water outlet INTEGRA Biosciences Page 9...

  • Page 16: Electricity And Water

    If condensation is visible on the MediaClave, do not switch on or operate the instrument for at least two hours. WARNING: If the MediaClave is to be operated with 400 V, 3 phases, the voltage network must be iso- lated with a circuit breaker power switch or by a suitable isolation device. 2.4.2 Water supply Three water connectors are situated on the MediaClave‘s rear panel.

  • Page 17: Filter And Tubing Installation

    : Wetness or wearing out of the filter may provoke a pressure test failure! 2.6 Moving the MediaClave 2.6.1 Moving procedure ATTENTION: Before moving the MediaClave, it must be cooled down, emptied and disconnected from the mains NOTE: Two persons are required to lift the MediaClave. •...
  • Page 18 MediaClave overview INTEGRA Biosciences Page 12...

  • Page 19: Operating Controls

    User Manual 3.4 Operating controls 3.1 Introduction This chapter gives needed information to prepare the MediaClave for every operation mode. 3.2 Preparation for different operational modes 3.2.1 Mode MEDIACLAVE 3.2.1.1 Installing the Rigid Temperature Sensor Unplug the flexible temperature sensor from the rear panel if installed...
  • Page 20 3.2.1.5 Filling the vessel In mode MEDIACLAVE, the heating water (coupling medium) filling level depends on the amounts of prod- uct. Fill the vessel with distilled or deionised water. Ensure that the water does not contain chalk or min- erals. The water level (with the cuvette inserted) must be approximately 7 cm below the upper edge of the vessel (approx.

  • Page 21: Mode Autoclave

    User Manual 3.4 3.2.2 Mode AUTOCLAVE NOTE: The vessel lid must be closed at all times during operation of the AUTOCLAVE programs! 3.2.2.1 Installing the flexible temperature sensor Unplug the rigid temperature sensor form the rear panel if installed Using an Allen tool, unscrew the rigid temperature sensor from the vessel lid Insert the flexible temperature sensor, and tighten with the Allen tool Plug in to the rear panel ATTENTION:...
  • Page 22 MediaClave overview 3.2.2.4 Specific configuration in program mode AUTOCLAVE NOTE : The program mode AUTOCLAVE is suitable to sterilise small amounts of medium in single con- tainers such as e.g. “Erlenmeyer flasks” or test-tubes. ATTENTION : These containers must be placed as shown in the figure below to ensure an error-free operation.

  • Page 23: Mode Waterbath

    3.3 Switching On and Off the MediaClave The MediaClave is switched on and off with the green power switch located on the right-hand side panel of the instrument. After switching on the instrument, it is automatically initialised and a self-test is per- formed.

  • Page 24: Soft Key Menu Options Explained

    MAIN menu options In the Main menu, programs can be directly started or modified in the PROGRAM menu. • Choose one of the three main operation modes or programs (MEDIACLAVE, AUTOCLAVE or WA- TER BATH). The MAIN menu appears •...

  • Page 25: Language Menu

    User Manual 3.4 3.3.2.4 EDIT menu options In the EDIT menu, the current program can be modified or adapted to suit specific requirements. • Press EDIT in the PROGRAM menu. The EDIT menu appears • Press CONTINUE to modify program parameters using the - and + keys •...

  • Page 26: Parameter Menu

    MediaClave overview 3.3.4 PARAMETER menu Instrument adjustments can be viewed or modified in the PARAMETER menu. NOTE: All instrument data is stored in NVRAM (Non-Volatile Random Access Memory). NVRAM retains all memory even when the power to the instrument is switched off. A battery automatically takes over and all modifications and instrument data are saved.
  • Page 27 Press SAVE to return to the PARAMETER ENTRY menu COMMUNICATION NOTE: A MediaClave device address can be selected from positions 1 to 16. From the PARAMETER ENTRY menu, press CONTINUE to scroll to and select COMMUNICATION. Press EDIT The SET HOST ADDRESS menu displays two options: •...
  • Page 28 The VENTILATION PARAMETER menu displays two options: • MEDIACLAVE (Default: 100°C) • AUTOCLAVE (Default: 105°C) Choose either MEDIACLAVE or AUTOCLAVE Press – or + to adjust the ventilation end temperature Press SAVE to return to the PARAMETER ENTRY menu COOLING NOTE: The minimum heating water (coupling medium) temperature can be adjusted between 5°C and...
  • Page 29 User Manual 3.4 TIME NOTE: The system clock is a 24-hour clock. The time style used is hh:mm. From the PARAMETER ENTRY menu, press CONTINUE to scroll to and select TIME Press EDIT The SET TIME menu displays two options: •...
  • Page 30 MediaClave overview INTEGRA Biosciences Page 24...

  • Page 31: Instruction For Use

    Instructions for use 4.1 Introduction The MediaClave is a device used for the production of culture media containing agar or culture nutrient fluids. Additionally, the MediaClave can be used as an autoclave or a water bath. This section of the manual is divided into three main parts: •...

  • Page 32: Program Operation

    MediaClave overview 4.3 Program operation 4.3.1 MEDIACLAVE program operation 4.3.1.1 Overview The flow of the MEDIACLAVE sterilisation cycle is as follows: → → → → System check Pressure test (excluding MEDIACLAVE program 14) Heating Sterilisation → → → → →...
  • Page 33 PROGRAM 15 (Chocolate Agar) is used for the production of chocolate agar. It runs in two cycles (sterilisation and boiling). ating Figure 4.1: The MEDIACLAVE PROGRAM [#] Screen Figure 4.1 illustrates the progress of the sterilisation cycle as displayed on the MEDIACLAVE PROGRAM [#] screen. 4.3.1.8 Selecting programs Press MEDIACLAVE on the start display (see section 3.4.1).
  • Page 34 MediaClave overview 4.3.1.9 Modifying programs ATTENTION: Read section 7.4 before modifying programs. Program 1 to 14 Follow steps 1–4 in section 4.3.1.8 Press EDIT to branch into the EDIT menu where the program can be modified (see section 3.4.2.4) Press CONTINUE to scroll to and select the sterilisation temperature Press –...
  • Page 35 MAIN menu appears. Open the safety cover and vessel lid (see section 3.2) Press MEDIACLAVE on the start display. The MEDIACLAVE PROGRAM [#] screen appears Press START Fill the cuvette with the medium to be prepared and enter the stirrer bar Install the cuvette in the vessel (see section 3.2)
  • Page 36 MediaClave overview 4.3.1.11 Flow of the MEDIACLAVE sterilisation cycle ATTENTION: Press STEP to interrupt the cycle and proceed to the next cycle step. The program cannot be interrupted during cooling. NOTE: A progress printout is printed every x seconds / minutes during the sterilisation cycle (x refers to the selected print cycle, see section 3.4.4.2).

  • Page 37: Autoclave Program Operation

    User Manual 3.4 The safety cover opens automatically NOTE: The actual and target dispensing times are displayed in the upper right of the screen. 11. Dispense the product (see section 4.3.1.7) 12. When the cuvette is empty, press END. The cycle ends 4.3.2 AUTOCLAVE program operation ATTENTION:...
  • Page 38 MediaClave overview Figure 4.2 illustrates the AUTOCLAVE cycle progress as displayed on the AUTOCLAVE PROG [#] ATTENTION: All AUTOCLAVE programs are similar. Unlike the MediaClave, there are no programs with special functions. Figure 4.2: The AUTOCLAVE PROG [#] Screen 4.3.2.7 Selecting programs Press AUTOCLAVE on the start display (see section 3.4.1).
  • Page 39 User Manual 3.4 Press CANCEL to reject all modifications and return to the PROGRAM menu 11. Press START to start the selected program (see section 4.3.2.9) 4.3.2.9 Starting and running programs ATTENTION: Ensure there is sufficient heating water in the vessel. If there is insufficient heating water in the vessel, the following alarmed message appears –...

  • Page 40: Waterbath Program Operation

    MediaClave overview • HEATING UP NOTE: The actual and target temperatures are displayed in the upper right of the screen. The target temperature must be reached in order for the cycle to progress. • STERILISING NOTE: The actual sterilisation temperature and countdown timer are displayed in the upper right of the screen.
  • Page 41 The vessel lid must remain open throughout the WATER BATH cycle. If the vessel lid is closed, the cycle is terminated and cooled to the heating safety temperature of 80°C ATTENTION: All 15 WATER BATH programs are similar. Unlike the MediaClave, there are no programs with special functions. 4.3.3.4 Selecting Programs Press WATERBATH on the Start Display (see section 3.4.1).
  • Page 42 MediaClave overview 4.3.3.6 Starting and running programs ATTENTION: Ensure there is sufficient heating water in the vessel. ATTENTION: Pressing END results in the following alarmed screen message – CYCLE IS BEING CON- CLUDED. The MAIN menu appears. ATTENTION: Install the cuvette (see section 3.2), and fill with approximately 2 L of water.

  • Page 43: Cleaning Instructions

    Paper towels 5.2.1.2 Cleaning procedure WARNING : Before starting the cleaning procedure, ensure the MediaClave is switched off and discon- nected from the mains. Clean and empty the vessel Switch off the MediaClave and disconnect it from the power source Visually check the instrument for obvious spills, paying particular attention to the following areas: •...

  • Page 44: Removing Residual Dirt

    Moisten a cleaning cloth with the liquid dishwasher detergent ATTENTION: Do not soak the cloth in the solution. Do not let the solution drip inside the MediaClave. Use the moistened cloth to clean all affected areas including vessel, cuvette and temperature sensor...

  • Page 45: Mediaclave Cleaning Menu

    5.3.2 Overview The CLEANING menu is illustrated in figure 2.8. In the CLEANING menu, the MediaClave can be emptied or cleaned by pressing the EMPTY or CLEAN soft keys. The MAIN MENU soft key is used to return to the Start Display.

  • Page 46: Clean Menu

    MediaClave overview 5.3.3.2 Emptying the MediaClave NOTE: The emptying duration depends on the volume of the vessel content. Open the safety cover and vessel lid Press EMPTY on the CLEANING menu. The EMPTY menu appears and certain messages progres- sively appear: •...
  • Page 47 User Manual 3.4 NOTE: If there is insufficient water in the vessel, the following alarmed message appears – TOO LITTLE HEATING WATER IN VESSEL. Press END to confirm this error. • VESSEL IS BEING HEATED NOTE: The actual and target temperatures are displayed in the upper right of the screen. The pre- programmed target temperature (see Section 3.4.4.2) must be achieved in order for the cleaning cy- cle to progress.
  • Page 48 MediaClave overview INTEGRA Biosciences Page 42...

  • Page 49: Maintenance

    NOTE: The appearance of a red strip at the edge of the paper indicates the need of a paper-replacement. Ensure that the MediaClave is switched on Remove the printer protection plexiglass and the magnetic front cover Press the form feed button to expel the last piece of paper...
  • Page 50 MediaClave overview Figure 6.1: The MediaClave printer Figure 6.2: Inserting the Printer paper roll INTEGRA Biosciences Page 44...

  • Page 51: Temperature Calibration

    • Connect the double Pt100 sensor to the temperature sensor inlet. There are two connector cables. One is connected to the MediaClave rear panel, the other one to the external instrument. • Compare the true temperature reading with the temperature reading on the display. If the displayed reading differs from the true reading more than defined by internal company directives, the tempera- ture measurement must be adjusted.

  • Page 52: Troubleshooting + Error Handling

    6.4.1 Possible solutions for problems Tables 6.1 and 6.2 can help to remedy certain problems that may be encountered when operating the MediaClave. These tables contain the symptom, possible cause and corrective action related to the par- ticular problem. Symptom...

  • Page 53: Error Handling

    The MediaClave software contains error-handling procedures that enable certain problems to be rectified without service intervention. If the MediaClave encounters a problem, it tries to rectify the problem by is- suing instructions to the user via the GUI. If the on-screen instructions are followed, the error can usually be rectified without service intervention.
  • Page 54 Displayed Text Description/Action WARNING: Description: Before starting operation, the PLEASE CLOSE COVER! MediaClave verifies the sealing of the safety cover. If the cover is opened, the user is informed. Reaction: Close the cover. WARNING! Description: In mode Water bath, the Media- PLEASE OPEN BOILER! Clave verifies the sealing of the vessel.

  • Page 55: Frequently Asked Questions

    The agar must be discarded. Continuing the program causes resumption at the beginning of the interrupted cycle. The MediaClave will take 30 seconds to restart. What causes an excess of agar to remain at the The length of the internal tube connected to the bottom of the cuvette at the end of a process dispensing port may be too short.
  • Page 56 MediaClave overview INTEGRA Biosciences Page 50...

  • Page 57: Accessories And Technical Specifications

    User Manual 3.4 Accessories and Technical specifications 7.1 Introduction The Technical Reference information provides additional information to the MediaClave • MediaClave accessories • Country versions • Software default values • Limitations and general notes • Port connector definitions • Progress printouts •...

  • Page 58: Country Versions

    7.4 Software default values 7.4.1 Overview This section informs about the different default program settings which comes with a new MediaClave. Furthermore, the default general values, which can be changed in the PARAMETER Menu, are listed. 7.4.2 MEDIACLAVE programs 1–13 (1–9 L of medium) PROGRAMS 1–13...

  • Page 59: Mediaclave Program 14 (Without Pressure Test)

    User Manual 3.4 7.4.3 MEDIACLAVE program 14 (Without pressure test) NOTE: This program is capable of preparing 1–9 L of liquid. This program performs steam disinfection but no sterilisation. PROGRAMS 14 Range Default Sterilisation temperature: 70 - 105°C 105°C Sterilisation time: 1 - 99 min.

  • Page 60: General Default Values

    7.5 Limitations and general notes 7.5.1 Overview • When operating the MediaClave in the MEDIACLAVE program mode, always follow the recommen- dations of the medium suppliers. Using non-specified values may influence the quality of the pre- pared medium. • To operate the MediaClave in the AUTOCLAVE program mode, special equipment (AUTOCLAVE kit) must be ordered (see section 7.2.1).

  • Page 61: Limit Values Within Mode Mediaclave

    WARNING: Modification of the pre-installed programs (see section 7.4) may influence the efficiency of the sterilisation/steam disinfection. Perform validation of altered programs before using as standard. 7.5.2 Limit values within mode MEDIACLAVE Program number Step in cycle Range Programs 1–13 Sterilisation temperature: 70–122°C...

  • Page 62: Input / Output Connectors

    7.6.1 Overview The MediaClave has three external ports which use 9-pin D-sub miniature connectors. The first serial RS232 port supports devices such as an external PC or the MEDIAJET. The second serial interface is intended for an external printer. (supported from software version 1.9.1) The third port provides status in- formation (safety cover lock, alarms).

  • Page 63: Second Serial Port Rs232

    The interface for an external printer is active, if additionally to the software version 1.9.1 a spe- cial cable (Order number 135245) is installed in the MediaClave. MediaClave instruments which are purchased after January 2006 have this cable built in by default! The Epson TM-U220B is a matrix pro- tocol printer.

  • Page 64: Interface For External Safety Cover Lock Switch And Alarm

    The buzzer built-in voltage may amount to 24 V DC. The contact is in an open state of rest and is separated galvanically. (That is, there is no electronic contact between the MediaClave and external power supply. This is realised by relays or opto-electronic parts.) The forced opening cover only operates with a battery and a maximum 24 V DC voltage.

  • Page 65: Progress Printouts

    7.7 Progress printouts 7.7.1 Overview There are two kind of printouts, the test print for test of internal and external printer and the detailed pro- tocol of the single program processes (MEDIACLAVE, AUTOCLAVE, WATER BATH) 7.7.2 Test print Item printed...

  • Page 66: Progress Print

    The actual program phases are printed • Heating • Temperature held (WATERBATH) • Sterilisation • Cooling • Addition (MEDIACLAVE program 15 only) • Heating (MEDIACLAVE program 15 only) • Boiling (MEDIACLAVE program 15 only) • Cooling (MEDIACLAVE program 15 only) • Dispensing STEP Button:...

  • Page 67: Mediaclave Physical Properties

    Feed tube dimensions: Internal diameter: 6 mm Thickness: 2 mm Product live span: Max. 10’000 sterilization cycles or max. 10 years Table 7.17: MediaClave physical properties 7.8.2 Installation data Water tubing: 1.5 m, 3/4“ with standard thread Maximum pressure of cooling water...
  • Page 68 MediaClave overview INTEGRA Biosciences Page 62...

  • Page 69: Manufacturer And Customer Service

    User Manual 3.4 Manufacturer and Customer Service 8.1 Manufacturer: INTEGRA Biosciences AG Tardisstrasse 201 CH-7205 Zizers Switzerland www.integra-biosciences.com info@integra-biosciences.com 8.2 Customer Service: Please contact your local INTEGRA Biosciences AG distributor. You will find their name and address on our web-site www.integra-biosciences.com. INTEGRA Biosciences Page 63...
  • Page 70 MediaClave overview INTEGRA Biosciences Page 64...

  • Page 71: Glossary

    Error messages that are accompanied by sound. SAGES AUTOCLAVE Program suite used for operation of MediaClave as a bench-top auto- clave. The user has a choice of 15 possible programs. CLEANING Programs associated with the cleaning and emptying of the Media- Clave.
  • Page 72 External method for calibrating the manometer (performed by an IBS service technician). PRESSURE TEST Built in safety function at the begin of a MEDIACLAVE cycle. PRINT CYCLE Program parameter that determines the frequency of progress print- outs during a program cycle (10 seconds – 10 minutes range).
  • Page 73 COVER from moisture. START DISPLAY Screen that appears when the instrument is switched on. This allows the user to select the six main options used by MediaClave (MEDIA- CLAVE, AUTOCLAVE, WATERBATH, CLEANING, LANGUAGE and PARAMETER). STEP This program function enables the operator to interrupt a cycle phase manually and proceed to the next phase in the program cycle.
  • Page 74 MediaClave overview INTEGRA Biosciences Page 68...

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