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Summary of Contents for Munro AW500SG
- Page 1 ANAEROBIC WORKSTATION AW500SG SINGLE GAS VERSION MANUAL 44-45 Burnt Mill | Elizabeth Way | Harlow | Essex | CM20 2HU | United Kingdom Telephone: +44 (0) 20 8551 7000 | Website: www.munroinstruments.com...
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Page 2: Table Of Contents
CONTENTS GENERAL ARRANGEMENT DRAWING OF ANAEROBIC WORKSTATION GENERAL SPECIFICATION GAS SUPPLIES ELECTRICAL SUPPLES INSTALLATION OF GAS AND ELECTRIC DRAWING ASSEMBLY INSTRUCTIONS NO. 1. DRAWING ASSEMBLY INSTRUCTIONS NO. 2. DRAWING SPECIAL FEATURES CHECKING THE INCUBATOR ATMOSPHERE FOR OXYGEN 10. PREPARING THE ANAEROBIC INDICATOR SOLUTION 11. -
Page 3: General Arrangement Drawing Of Anaerobic Workstation
GENERAL ARRANGEMENT OF MUNRO AW500SG ANAEROBIC CABINET 50 1/2” (1283mm) 32” (813mm) 15” (381mm) Control panel Condensation Plate Cooling air inlet Clear acrylic window Anaerobic indicator bottle Manually sealed interlock doors Bubble bottle Flushing interlock 60 Petri dish capacity Single plate entry Bare hand operation gauntlets 27 ½”... -
Page 4: General Specification
GENERAL SPECIFICATION MODEL AW500SG LOCK CAPACITY 60 Petri Dishes MAIN CHAMBER INCUBATION CAPACITY 400 Petri Dishes DOOR SEALING Manual Mixed Gas (10% H2 + 10% CO2 + 80% N2) OXYGEN REMOVAL FROM AIRLOCK Positive Pressure flushing with catalyst FABRICATION MATERIAL... -
Page 5: Gas Supplies
GAS SUPPLIES This Anaerobic Cabinet requires one gas cylinders fitted with a two stage regulator. The output side of the regulator should have a range of 0 - 50 p.s.i. (3.3 bar). The gas cylinder should contain: 10% Hydrogen + 10% Carbon Dioxide + 80% Nitrogen CAUTION: GAS CONTAINING MORE THAN 10% HYDROGEN SHOULD NOT BE USED UNDER ANY CIRCUMSTANCES... -
Page 6: Installation Of Gas And Electric Drawing
INSTALLING THE GAS AND ELECTRICAL SUPPLY TO THE AW500SG WORKSTATION 2 stage pressure regulator Secondary gauge showing pipeline Primary gauge showing pressure set between 1.6 and 2.5 Bar pressure in bottle Low gas pressure sufficient to switch low gas pressure indicator indicator “OFF”... -
Page 7: Assembly Instructions No. 1. Drawing
AW500SG ASSEMBLY INSTRUCTIONS NO. 1 DRAWING Hang indicator pump here and plug into adjacent socket Clip anaerobic indicator bottle here Place conditioning sachet and palladium catalyst sachet in channel under shelf in order shown Catalyst Conditioner Place condensation collection bottle here inside incubator and... -
Page 8: Assembly Instructions No. 2. Drawing
AW500SG ASSEMBLY INSTRUCTIONS NO. 2 DRAWING Insert “Mains Failure” battery and battery Seal gauntlets with “bungs” holder here when not in use Dust both “Wrist Cuffs” with powder before inserting arms into gauntlets Arm Ports Powder Gauntlet hooks Gauntlet holder... -
Page 9: Special Features
THE MUNRO AW500SG ANAEROBIC WORKSTATION SPECIAL FEATURES Many special features have been designed into the MUNRO Anaerobic Workstation that cannot be found on any other cabinet. All these have been built in for safety, economy of operation and to make it easier and more comfortable for the operator to use. -
Page 10: Preparing The Anaerobic Indicator Solution
PREPARING THE ANEROBIC INDICATOR SOLUTION Make up the Anaerobic Indicator Solution from the two ingredients provided, sufficient to fill the bottle about 1/2 full using nine parts of solution "A" and one part of solution "B" Now place a cap lightly on the neck of the bottle and place it in a bath of water and bring it to the boil. The solution will change from being blue to colourless. -
Page 11: Commissioning The Cabinet
COMMISSIONING THE CABINET Check all the following points before starting to commission the workstation. Switch "OFF" the cabinet at the Mains ELCB switch Open the Mixed Gas Regulator, set the secondary gauge to 30 psi (2.0 Bar) at the inlet to the unit with the gas flowing Ensure that the Gas Trap Bubble Bottle is filled up to the water level line and the gauntlets and bungs are both fitted to the cabinet. -
Page 12: Checking The Gas Flow
CHECKING THE GAS FLOW To check the Gas Flow into the Workstation the following equipment is required: 1. Gas Flow Meter (0 – 30 Litres per minute) 2. Pressure Gauge (0 – 3.3 Bar) Connect up the equipment as shown and start the “Lock Flush” Inlet Pressure Switch Flow Meter Pressure Gauge... -
Page 13: Transferring Items Into The Incubator
TRANSFERRING ARTICLES IN AND OUT OF THE CABINET TRANSFERRING ITEMS INTO THE INCUBATOR The Workstation is supplied with ten stainless steel Petri Dish Holders, each capable of holding ten Petri Dishes. These are used for transferring dishes into and out of the cabinet and stacking them whilst they are being incubated. -
Page 14: Removal Of Condensation From Inside The Incubator
THE REMOVAL OF CONDENSATION FROM INSIDE THE INCUBATOR Water vapour accumulates inside the incubator atmosphere from two sources, firstly from the evaporation of the media in the Petri dishes and secondly by the hydrogen and the traces of oxygen being linked together by the palladium catalyst. -
Page 15: The Gas Circulation System
THE GAS CIRCULATION SYSTEM The fan units pick up the cooler incubator atmosphere which has been forced to the top of the incubator and first passes it over the warm heater. This warmer gas is now forced down the channel at the back of the cabinet then it passes under the bottom shelf of the incubator where it first meets the sachets of Conditioner and then the sachets of Catalyst. -
Page 16: Working Inside The Cabinet With Bare Hands
WORKING INSIDE THE CABINET WITH BARE HANDS Although the MUNRO cabinet can be used with long sleeved gloves it is much easier to work with bare hands. This bare hand facility is achieved in a very simple but effective way. The Bare Hand Gauntlet Assembly consists of four components as shown in diagram 1. -
Page 17: General Maintenance
GENERAL MAINTENANCE The MUNRO Workstation, unlike other units, uses the energy contained in the gas bottles to do most of the work required. This means that there are no evacuation or circulation pumps to maintain. Many of the components used in the construction of the unit are available "off-the-shelf" in almost every part of the world. -
Page 18: Changing The Catalyst And Conditioner
CHANGING THE CATALYST AND CONDITIONER The sachet of Catalyst and the sachet of Conditioner which are housed beneath the bottom shelf of the incubator will require replacing at a period in excess of one year. If for any reason the sachet of catalyst °... -
Page 19: Test Report
TEST RECORD SHEET MODEL AW500SG SERIAL NO 7403 CUSTOMER PUNMICRO FLUSH CYCLE 4 SECONDS ON 2 SECONDS OFF FLUSH DURATION 5 MINUTES MIXED GAS FALLING CUT OUT PRESSURE MIXED GAS RISING SWITCH ON PRESSURE MIXED GAS FLOW L / MIN... -
Page 20: Declaration Of Conformity
Declaration of Conformity Equipment Anaerobic Incubator type AW500SG Manufacturer MUNRO Ltd Address 44-45 Burnt Mill, Elizabeth Way, Harlow, Essex, CM20 2HU, UK Description of Equipment Incubator for the environmentally controlled incubation of anaerobic bacteria. I certify that the apparatus identified above conforms to the requirements of Council Directive...
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