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Cole Parmer Cryo-Blade CG-900 Operating Manual

Cryogenic grinder
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Operating Manual
CG-900 Cryo-Blade™
Cryogenic Grinder
coleparmer.com

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Summary of Contents for Cole Parmer Cryo-Blade CG-900

  • Page 1 Operating Manual CG-900 Cryo-Blade™ Cryogenic Grinder coleparmer.com...

  • Page 2: Table Of Contents

    Operating Manual Contents Introduction ..............................4 The Cryo-Blade ................................4 Accessories ................................5 Main Features ................................6 Precautions ..............................7 Installation ..............................8 Location ..................................8 Unpacking ................................10 Connection – Electrical ............................10 Connection – Exhaust ............................10 Connection – Liquid Nitrogen ..........................11 Leveling ..................................12 Setting Up ................................
  • Page 3 Operating Manual Special Parameters ............................. 28 Accessing the Special Parameters ........................28 Parameters Descriptions ............................ 28 10.0 Troubleshooting ............................29 11.0 Periodic Inspection .............................29 11.1 Warning ................................... 30 11.2 Inspection Schedule Table ..........................31 12.0 Service Operations ............................31 12.1 Warning ..................................31 12.2 Back Panel Removal .............................

  • Page 4: Introduction

    Operating Manual Introduction Thank you for choosing the CG-900 Cryo-Blade Cryogenic Grinder from Cole-Parmer. To ensure years of reliable, efficient, and safe use of this instrument, please read this manual thoroughly and keep it in a safe and handy place for future reference.

  • Page 5: Accessories

    Operating Manual The bowl of the Cryo-Blade is composed of an outer insulated shell and an inner stainless steel bowl. The inner bowl can be removed for ease of cleaning and handling. A new inner bowl (order separately) can then be loaded in the outer shell to be ready to use.

  • Page 6: Main Features

    Operating Manual Main Features Accuracy • Automated liquid nitrogen dispensing and grinding • Use a load cell to measure the sample weight to control the required amount of liquid nitrogen or to monitor the dry ice remaining • Temperature sensor (thermocouple type K) to monitor temperature of the bowl during cooling and grinding •...

  • Page 7: Precautions

    Operating Manual Precautions High voltage Disconnect power cord before attempting any cleaning, maintenance or repair operation. Be careful that no liquid infiltrates into the unit’s casing. Heavy instrument It is advisable that at least two people move this instrument to avoid injuries. Do not drop instrument.

  • Page 8: Installation

    Operating Manual 3.0 Installation Please read the following section for proper commissioning of your instrument. Do not hesitate to contact Cole-Parmer with any question you might have regarding this crucial step. IMPORTANT: The instrument can be carried by its bottom plate. It is advisable that two or more people carry this instrument to avoid injuries.
  • Page 9 Operating Manual Liquid nitrogen handling Liquid nitrogen cylinder should be kept in a well-ventilated area. Leak and safety valve will release nitrogen vapor and can be a serious asphyxiating hazard. When moving liquid nitrogen cylinders always use a specially designed cylinder cart and follow instructions of the cylinder manufacturer.

  • Page 10: Unpacking

    Operating Manual Unpacking The CG-900 Cryo-Blade mechanism and cabinet are shipped assembled, and carefully packed to avoid damage during shipping. Any visible damage to the shipping container should immediately be reported to the carrier. If there is no visible damage, remove all packing documents from the exterior of the box, and after completion of your inspection, file in your records.

  • Page 11: Connection - Liquid Nitrogen

    Operating Manual Vertical exhaust cap Exhaust collector Nitrogen input Hose anchor Back View Connection – Liquid Nitrogen The liquid nitrogen should be connected to the unit using a hose (not included), safety valve and fitting with the unit as shown on the pictures below. We recommend inserting the hose in the insulation tube included. This will minimize the condensation and water spillages during operation.

  • Page 12: Leveling

    Operating Manual Leveling The front foot of the unit is connected to a sensing device to monitor liquid nitrogen dispensed and measure sample weight. To have the best accuracy, it is important that the unit be reasonably leveled. If in doubt, place a bubble spirit level on top of the unit and adjust the instrument’s back feet to compensate for possible slant.

  • Page 13: Cryogenic Basis

    Operating Manual Cryogenic Basis When cooled to cryogenic temperatures, almost all materials freeze and become hard and brittle. This property can be used to break them into a fine homogeneous powder. The powder can then be analyzed with the method of your choice.
  • Page 14 Operating Manual Locking the bowl When inserting the bowl in the Cryo-Blade, make sure the bowl is pushed completely to the back. If incorrectly inserted, it will be impossible to lock the bowl. When the bowl is correctly inserted, the user can lock the bowl in place by moving the locking lever toward the back.

  • Page 15: Grinding Methods

    Operating Manual Inner bowl Insulated outer bowl Locking lever WARNING: Wear protective gloves to remove or when handling the inner bowl to prevent frostbite risk. Bowl and lid cleaning Even though the bowl is insulated, ice can form on or around its components due to the extremely cold temperature involved in the process.
  • Page 16 Operating Manual Preconditioning The first step executed by the unit is dispensing the liquid nitrogen to cool down the sample and the vessel used to grind it. If dry ice protocol is used, the dry ice is added by the user manually and the liquid nitrogen will not be used. The preconditioning step is completed when the preconditioning temperature and the quantity of liquid nitrogen are reached within the maximum allowed time.

  • Page 17: Liquid Nitrogen Dispensing

    Operating Manual Liquid Nitrogen Dispensing The Cryo-Blade automatically dispenses the liquid nitrogen used to cool the sample prior to grinding, reducing the risk generated by the manipulation of this substance. A cryogenic solenoid valve is used to control the dispensing of the liquid nitrogen. A temperature sensor (thermocouple type K) is located underneath the inner bowl and connected to the unit by inserting the bowl in the instrument to monitor the temperature inside the bowl.

  • Page 18: Safety Features

    Operating Manual Safety Features To run, the bowl needs to be inserted and locked in the unit using the locking lever. Unlocking the bowl while the unit is running will disengage the bowl from the unit and stop the grinding process. When the bowl is correctly locked in the unit, the ready status in the upper area of the main screen will show that the unit can be started (see picture below).
  • Page 19 Operating Manual This icon indicates the cooling step. When pressed (grayed), it deactivates the cryogenic steps (preconditioning, cooling and evaporation). When activated, the cooling duration can be adjusted using the decrease and increase buttons. This icon is used to select the mode of operation (liquid nitrogen or dry ice). In liquid nitrogen mode, it identifies the quantity of liquid nitrogen to dispense and maintain during the preconditioning and cooling steps.

  • Page 20: Loading A Protocol

    Operating Manual The Save button is used to log the current program and its parameters into memory. See page 25, “Managing Grinding Methods” for details. This button is used to access Global parameters. See page 26, “Global Parameters” for details. This zone is used to select the current active grinding method.

  • Page 21: During A Cycle

    Operating Manual During a Cycle While the instrument is running, the main screen will display additional information and buttons, as well as the number of the step being currently executed and a countdown showing the total time remaining to the grinding protocol.

  • Page 22: General Comminution Using Liquid Nitrogen

    Operating Manual This icon shows the temperature of the inner bowl. The temperature sensor is located underneath the bowl and allows the system to measure a temperature down to -160 °C. When the bowl is not inserted or the temperature is below -160 °C, the system indicates a temperature of -160 °C.

  • Page 23: General Comminution Using Dry Ice

    Operating Manual 9. The Cryo-Blade motor then starts and reaches the speed set for the grinding and maintains it for the set time. 10. Once the timer has elapsed, the motor slows to a stop, the screen will return to main screen to inform the user that the cycle has been completed.

  • Page 24: Programming The Cryo-Blade (Advanced)

    Operating Manual 10. The Cryo-Blade motor then starts and reaches the speed set for the grinding and maintains it for the set time. 11. Once the timer has elapsed, the motor slows to a stop, the screen will return to main screen to inform the user that the cycle has been completed.

  • Page 25: Managing Grinding Methods

    Operating Manual This icon indicates that you can now modify the grinding protocol parameters and manage the grinding methods (i.e. copy, delete and save). You are now in “advanced mode”. NOTE: It is not possible to modify the parameters in the preset program, and so even entering the correct password will not “unlock”...

  • Page 26: Preparing A Grinding Program

    Operating Manual Preparing a Grinding Program To build your first grinding protocol, you must first select the preset protocol template or any existing protocol (refer to “Loading Protocol” on page 20) that will be used as a starting point to design your own protocol. Copy it under your desired name, as described above.

  • Page 27: Options

    Operating Manual Options This section allows you to pick from a selection of available interface languages. The duration parameters are used to determine the relation between the sample mass and the duration to execute during the cooling and grinding step in automatic mode.

  • Page 28: Special Parameters

    Operating Manual This box shows the serial number of the Cryo-Blade unit. The number to the right of the “odometer” icon shows the total number of grinding cycles that the Cryo-Blade unit has performed. 9.0 Special Parameters This section presents an advanced interface window that can be used to adjust the special parameters on the Cryo-Blade.

  • Page 29: Troubleshooting

    Operating Manual 10.0 Grinding Troubleshooting This section presents the most common grinding-related problems. For specific assistance, please do not hesitate to contact us (see page 36,” Contacting Cole-Parmer”). Problem Possible Cause Possible Solution Make sure the power cord is plugged into outlet. No power at all Check outlet for power and correct voltage as Unit does not turn on...

  • Page 30: Warning

    Operating Manual 11.1 Warning IMPORTANT: Some of the procedures described in the following pages imply a risk of death by electrocution; those procedures shall be executed only by trained personnel. 11.2 Inspection Schedule Table Action Frequency Checkpoint Description (if problem found) Clean bowl and lid with soap and water and Clean bowl and lid —...

  • Page 31: Service Operations

    Operating Manual 12.0 Service Operations This section describes tasks that are performed on a regular basis and are performed to adjust or repair a malfunctioning system of the instrument. Some sections also describe initial operations, which need to be performed before first using the unit. Should you have any question, or need further assistance, please do not hesitate to contact us (see page 36, “Contacting Cole-Parmer”).

  • Page 32: Left Panel Removal

    Operating Manual 12.3 Left Panel Removal In order to gain access to the exhaust system, it is necessary to remove the left panel. IMPORTANT: Always disconnect the instrument from the power outlet when working with panels removed. 240 Volts inside! Risk of electrocution! Disconnect the instrument from the wall outlet.

  • Page 33: Right Panel Removal

    Operating Manual 12.4 Right Panel Removal In order to gain access to motor and belt, it is necessary to remove the right panel. IMPORTANT: Always disconnect the instrument from the power outlet when working with panels removed. 240 Volts inside! Risk of electrocution! Disconnect the instrument from the wall outlet.

  • Page 34: Firmware And Program Transfers

    Operating Manual 13.0 Firmware and Program Transfers 13.1 Preset Protocols This section lists the methods that are factory programmed into the CG-900 Cryo-Blade. It is not possible for the user to alter or delete them. Note that Cole-Parmer cannot be held responsible for any damage to unit and the bowl incurred by the use of those methods.

  • Page 35: Technical Specifications

    Operating Manual Restore programs, firmware or upgrade firmware If you want to restore a backup that you have made yourself, simply insert the USB drive that contains your backup files into the USB port of the unit. 2. If you have obtained a new firmware by email, extract the directory structure and files you have received in the root directory of a blank USB drive.

  • Page 36: Warranty

    Operating Manual 15.0 Warranty Cole-Parmer guarantees its products and new equipment against defects in materials or workmanship for three (3) years from the date of original shipment. Repairs, replacements, or parts are guaranteed for 30 days or for the remaining original warranty period (whichever is greater) for the item that was repaired or replaced. Items not produced by Cole-Parmer carry the manufacturer’s warranty only.

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