NuAire LabGard ES Operation And Maintenance Manual
  1. Manuals
  2. Brands
  3. NuAire Manuals
  4. Laboratory Equipment
  5. LabGard ES
  6. Operation and maintenance manual

NuAire LabGard ES Operation And Maintenance Manual

Energy saver
Hide thumbs
Table of Contents

Advertisement

Quick Links

LabGard® ES Energy Saver
Class II, Type A2 Animal Handling
Biosafety Cabinet
Models
NU-640-400E/500E/600E
Bench/Console
Operation and Maintenance Manual
November, 2021
Revision 4
NU-400E/500E/600E Series A
Manufactured By:
NuAire, Inc.
2100 Fernbrook Lane
Plymouth, MN 55447
Toll-Free: 1-800-328-3352
In Minnesota: (763)-553-1270
Fax: (763)-553-0459
OM0292

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the LabGard ES and is the answer not in the manual?

Questions and answers

Subscribe to Our Youtube Channel

Related Manuals for NuAire LabGard ES

Summary of Contents for NuAire LabGard ES

  • Page 1 Class II, Type A2 Animal Handling Biosafety Cabinet Models NU-640-400E/500E/600E Bench/Console Operation and Maintenance Manual November, 2021 Revision 4 NU-400E/500E/600E Series A Manufactured By: NuAire, Inc. 2100 Fernbrook Lane Plymouth, MN 55447 Toll-Free: 1-800-328-3352 In Minnesota: (763)-553-1270 Fax: (763)-553-0459 OM0292...
  • Page 2 Other reference and guideline materials are available through the following web sites; www.hc-sc.gc.ca www.cdc.gov/od/ohs/ www.absa.org www.cabs-acsb.ca www.ebsaweb.eu www.inspection.gc.ca www.who.int www.biosafety.be www.hse.gov.uk www.nsf.org www.cetainternational.org www.nuaire.com OM0292 Page 2 of 57 Rev 4 November2021...
  • Page 3 A copy of the original factory test report is also appended to this manual. In case this manual and/or test report is lost or misplaced, NuAire retains a copy in our files. A replacement copy can be obtained by calling or writing NuAire, Inc.

  • Page 4: Table Of Contents

    LabGard® ES Energy Saver Class II, Type A2 Animal Handling Biosafety Cabinet Models NU-400E/500E/600E Operation and Maintenance Manual Table of Contents Section No. 1 ............. General Information Section No. 2 ............. Models and Features Section No. 3 ............. Warranty Section No. 4 ............. Shipments Section No.
  • Page 5 Biosafety Cabinet Models NU-400E/500E/600E Manufactured by: NuAire, Inc. - Plymouth, Minnesota, U.S.A. General Information 1.1 Description The LabGard® ES Model NU-640 Animal Handling Biosafety Cabinet (AHBSC) is a bench/table top model, optionally available with a base support stand, for operation as a console model. The LabGard® ES model NU-640 utilizes an Energy Saver DC ECM motor optimally determined forward curved fan for each model size/width to maximize both energy efficiency and filter loading capacity.
  • Page 6 • Should you encounter problems that are not detailed adequately in the operating instructions, please contact your NuAire Representative of NuAire technical Services. 1.3 Explanation of Symbols Safety alert symbol indicates a potentially hazardous situation which, if not avoided, could result in death WARNING of serious injury.
  • Page 7 OM0292 Page 7 of 57 Rev 4 November2021...
  • Page 8 Models and Features The model NU-640, LabGard® ES, Class II, Type A2 Animal Handling Biosafety Cabinet is manufactured in three sizes: 4 ft. (1.2m) , 5 ft. (1.5m) , and 6 ft. (1.8m). OM0292 Page 8 of 57 Rev 4 November2021...
  • Page 9 OM0292 Page 9 of 57 Rev 4 November2021...
  • Page 10 OM0292 Page 10 of 57 Rev 4 November2021...
  • Page 11 OM0292 Page 11 of 57 Rev 4 November2021...
  • Page 12 However, damage can occur in any shipment and the following outlines the steps you should take on receipt of a NuAire LabGard® ES cabinet to be sure that if damage has occurred, the proper claims and actions are taken immediately.

  • Page 13: Location

    CDC/NIH and NSF, it is strongly recommended that the cabinet be exhausted to the outside. NuAire offers a canopy type of exhaust transition, which will capture the exhaust efflux from the cabinet.

  • Page 14: Set-Up Instructions

    5.2.1 Gas Service NuAire doesn't recommend the use of natural gas within the AHBSC, but if gas service is determined to be necessary for the application, appropriate safety measures must take place. All NuAire AHBSC's have precautionary warning labels that say the following:...
  • Page 15 OM0292 Page 15 of 57 Rev 4 November2021...
  • Page 16 5.2.2 Plumbing Services Service ball valves with the type of service specified by the removable button on the handle are located in the work zone. The service ball valves are not recommended for pressure over 75 p.s.i. (5.2 BAR). Reducing valves should be installed external to the cabinet if necessary.

  • Page 17: Testing Methods And Equipment

    5.3 Testing Methods and Equipment After installation and prior to use, NuAire recommends that the cabinet be tested or commissioned to factory standards. As part of testing, the certifier should go through the following initial checklist to assure all aspects of the AHBSC installation are complete and ready for testing.
  • Page 18 LabGard® ES Energy Saver Class II Type, A2 Animal Handling Biosafety Cabinet Models NU-400E/500E/600E Catalog Number Catalog Number NU-640-400 NU-640-500 NU-640-600 Nominal 4 foot (1.2m) Nominal 5 foot (1.5m) Nominal 6 foot (1.8m) Performance Specifications NSF/ANSI 49 NSF/ANSI 49 NSF/ANSI 49 1.

  • Page 19: Aeromax™ Control System

    Operating the NU-640 6.1 Aeromax™ Control System 6.1.1 Overview The Aeromax™ control system is designed to service the control requirements of the LabGard® ES NU-640 Biosafety Cabinet. The Aeromax™ control system consists of an electronic module that will perform the following functions: •...
  • Page 20 OM0292 Page 20 of 57 Rev 4 November2021...
  • Page 21 6.1.2 Front Panel The control system front panel contains the following functions described in detail (see Drawing BCD-16521). 6.1.2.1 Blower Keys The blower key controls the ON/OFF power to the blower. LED above key indicates: full green for blower on, blinking green for blower pending and full red for blower alarm.
  • Page 22 6.1.3 Aeromax™ Control System Power After the LabGard® ES NU-640 is plugged into the appropriate facility line power the control system will power up. The control panel will also indicate the power up status by blinking the red alarm LED. Pressing any key will acknowledge the power up status and turn off the blinking red alarm LED.
  • Page 23 6.1.8 Operator Accessible Functions 6.1.8.1 Access and Navigation To access the operator accessible functions, • Press and hold the key, then enter the 3 key sequence for the desired function, then release key and follow each instruction set.  Note: Pressing the key at any time will abort and exit the process without saving any changes made.
  • Page 24 6.1.8.3 Blower Password The blower on/off password allows the cabinet user to place a 3 key sequence requirement to turn the blower on or off. The 3 key sequence for the blower password will be a combination of the hidden and blower keys. •...

  • Page 25: Operating Guidelines

    6.2 Operating Guidelines The intent herein is to present general operational guidelines that will aid in the use of the Animal Handling Biosafety Cabinet (AHBSC) to control airborne contaminants of low to moderate risk as stated in Technical Report No. FPS 56500000001, prepared by Dow Chemical U.S.A.
  • Page 26 6.2.4 Utilize Unidirectional Air Flow The operator must keep two important facts in mind: (1) The air, as supplied to the work area through filters from the top, is contaminant free and (2) Airborne contamination generated in the work area is controlled by the unidirectional flow of parallel air streams in a top-to-bottom direction.

  • Page 27: Operating Sequence

    6.3 Operating Sequence 6.3.1 Start Up Turn on cabinet blower and lights and check air intake and exhaust portals of the cabinet to make sure they are unobstructed. The electronic airflow control system will automatically control airflows to specified setpoints. However, upon filter loading, the cabinet may be required to be re-balanced or filters replaced.

  • Page 28: Ergonomics

    6.3.6 Terminal Purging and Wipe Down Following completion of work, allow the cabinet to run for 2-3 minute period without personnel activity to purge the cabinet. A surface disinfection of the interior surfaces (see Cleaning Procedures section) should be repeated after removal of all materials, cultures, apparatus, etc.
  • Page 29 Further information is available at the following: http://www.parrinst.com/wp- content/uploads/downloads/2011/07/Parr_Stainless-Steels-Corrosion-Info.pdf NOTE: NuAire does not offer any product warranty with respect to cleaning material compatibility. USE AT YOUR OWN RISK! The information provided above is from raw material suppliers and known general source documents for use to develop application cleaning SOP’s.

  • Page 30: Decontamination

    6.6.3 Assembly Replace front grill (if removed). b. Replace the work tray and carefully tighten the thumbscrews. Replace perforated metal diffuser screen over the underside of the supply HEPA filter. d. Wipe down all exposed surfaces of the work area with 70% isopropyl alcohol. Prepare for aseptic operation.
  • Page 31 LABORATORY EXPOSURE TO DECONTAMINATION GAS. 9. Perform decontamination procedure. Please consult with NuAire, Inc. about any unique contamination problems. Normally, no preventive maintenance is required on the interior of the cabinet (i.e., the area behind the access panel containing the HEPA filters and motor (blower assembly). All required adjustments in order to maintain proper cabinet airflows are external to the cabinet interior.

  • Page 32: Hepa Filter/Motor Replacement

    7.3 HEPA Filter/Motor Replacement The HEPA Filters do not need replacement under normal usage and barring an accident (a puncture), until the efflux velocity cannot be maintained or the access inflow velocity cannot be maintained at 100 LFPM (.51 m/s) (min.). This may permit the average downflow velocity to be as low as 55 LFPM (.28 m/s) as long as no point falls below 20% of the average downflow velocity.
  • Page 33 Step 4: Filter Installation When installing new filters, USE ONLY NUAIRE SPECFIED FILTERS FOR REPLACEMENT. Description: Supply HEPA Filter Exhaust HEPA Filter Efficiency: 99.99% @ 0.3 Micron 99.99% @ 0.3 Micron 100 fpm @ .55  .05" w.g. per sq. ft.
  • Page 34 To install the supply filter, simply reverse the procedure outlines in Step 3a, above.  Note: Be sure to open the choke plate fully before inserting the filter into the tray. This will assist in adjusting the airflow. b. To install the exhaust filter, apply a thin layer of silicone grease to the top and bottom gaskets of the filter and carefully insert into the exhaust choke tray.

  • Page 35: Sliding Window Replacement And Manual Adjustment

    7.4 Sliding Window Replacement and Manual Adjustment The sliding window replacement is accomplished by removing the front decorative panel, control center, and window glide assemblies. The sliding window adjustment may be required due to everyday use over the life of the cabinet. Both window glides are adjustable by a set screw and tension screw method.
  • Page 36 • Select motor control function/type Press and hold key, then press light – light – light keys sequentially. LED indicators above both blower (red) and light keys will blink fast. Review motor control function/type and change it desired as described below. Press ↑...
  • Page 37 Since it has been NuAire's experience that the filters may not "load" evenly, choke adjustments may be necessary for proper cabinet airflow balance and performance.
  • Page 38 7.5.3.1 Downflow Calibration Step 1: Place a velometer in the cabinet workzone on the horizontal plane 4 inches (102mm) above the bottom edge of the viewing window. Spot check several points on the recommended downflow velocity test grid in table 7.0 Step 2: If necessary, enter active blower speed adjustment.
  • Page 39 7.5.3.2 Inflow Calibration Step 1: Measure the inflow velocity using the recommended procedure found in Table 7.0. If necessary, adjust the exhaust filter choke, located under the front decorative panel, to achieve the correct average inflow velocity within the stated range of 105 ± 5 LFPM (.53 ± .025 m/s). •...
  • Page 40 7.5.3.3 PresurFlow™ Alarm Set Points The PresurFlow™ alarm setpoints are based on the calibration setpoint. Once the calibration setpoint is entered, based on a nominal inflow velocity of 105fpm (.53mls) the associated pressure sensor value is entered as the nominal pressure value. High and low alarm setpoints are factory verified and set if needed at 125fpm (.63mls) and 85fpm (.43mls).
  • Page 41 7.5.3.4 PresurFlow Alarm Verification The PresurFlow Alarm setpoints are based on the calibration setpoint. Once the calibration setpoint is entered, the Alarm setpoint offset pressure values will align from the calibration pressure value. The high or low alarm setpoint can be verified by measuring inflow volume/velocity while adjusting blower up or down within the Alarm Verification menu.
  • Page 42 Table 7.0 Recommended Measurement Methods for Cabinet Downflow & Inflow A. Downflow Measurement Recommended Instruments: TSI 8355 Thermo anemometer b. Procedure: Supply filter efflux is measured on a grid, in a horizontal plane 4 inches (102mm) above the bottom edge of the window.
  • Page 43 Alternate Procedure: The alternative procedure to determine inflow velocity uses a thermo anemometer in a constricted window access opening of 3 inches (76mm) use cardboard to lower the window access opening to 3 inches (76 mm). Inflow air velocity is measured in the center of the constricted opening 1-1/2 inches (38mm) blow the top of the work access opening on the following specified grid.

  • Page 44: Hepa Filter Leak Test

    7.6 HEPA Filter Leak Test In order to check filter and filter seal integrity, the HEPA filter media and seals must be directly accessible by the measuring instrument. The challenge material (i.e. PAO) should be supplied in the rear center of the workzone over the intake slots. The upstream challenge port being common for both filters is located under the work surface with a red cap.

  • Page 45: Airflow Smoke Pattern Test

    7.7 Airflow Smoke Pattern Test The airflow smoke pattern test is performed using a smoke source (i.e. smoke tubes) in and around the cabinet workzone and access opening to determine a visual representation of the cabinet’s containment performance. To perform the test, the smoke source should be passed through the following areas: A smoke source shall be passed: 1.

  • Page 46: Cleanliness Classification Test For Pharmacy Application

    7.9 Cleanliness Classification Test for Pharmacy Application If this cabinet is going to be used within pharmacy, per USP797 , the cabinet must be tested to assure compliance to ISO 14644-1:2015, Cleanrooms and Associated Controlled Environments, Part 1: Classification of Air Cleanliness .

  • Page 47: Main Control Board Description And Replacement

    7.10 Main Control Board Description and Replacement To access the main control board for fuse or board replacement, remove screws at each upper side of the control center and allow the control center to rotate down, resting on the safety straps. Now the main control board is exposed for service.
  • Page 48 7.10.4 Aeromax™ Control System Reset The Aeromax™ control board has a reset function available for Qualified Service Personnel. Master Reset-Resets all calibration, cabinet size, motor type/function and option settings back to their default condition. It is recommended to perform a master reset upon installation of replacement control board or if there seems to be intermittent functional abnormalities.
  • Page 49 8.1 Error Message Troubleshooting Guide Error Message Error Description Correction Check light fuse on main control board. Check LED lamps. Cabinet LED lights Blue LED above light key indicates the lamp should Check voltage coming out of main control board to won’t Turn on be on.
  • Page 50 8.2 Option Parameters The option parameter menu allows A QUALIFIED TECHNICIAN to configure several different optional parameters per the menu as described below. 8.2.1 Sync Function with Active Blower To access the option parameter menu, perform the following: • Press and hold key, then press hidden - Blower - LED keys sequentially.
  • Page 51 Require Password – Normally it is not required to use a password (i.e. 3 key press sequence of the blower and hidden key). If the option is turned on, it would be required to use the correct password to turn on the blower. The default password once turned on is blower- hidden-blower keys in sequence.
  • Page 52 Remote Contacts The NU-640 has several contact closures for remote sensing of various functions. 9.1 Fan Relay The fan relay contacts are normally open and closed contact closure outputs that are activated whenever the blower key is pressed and the blower key LED indicator is on or blinking. Contact ratings are 250 VAC maximum at 2 Amps. 9.2 Alarm Relay The alarm relay contacts are normally open and closed contact closure outputs which are activated whenever an airflow alarm condition occurs.
  • Page 53 10.0 Electrical/Environmental Requirements 10.1 Electrical (Supply Voltage Fluctuations Not to Exceed +/- 10%) *NU-640-400 115 VAC, 60 Hz, 1 Phase, 14 Amps *NU-640-500 115 VAC, 60 Hz, 1 Phase, 16 Amps *NU-640-600 115 VAC, 60 Hz, 1 Phase, 16 Amps *UL/UL-C Listed 10.2 Operational Performance (for indoor use only) Environment Temperature Range:...
  • Page 54 11.0 Disposal and Recycle Cabinets that are no longer in use and are ready for disposal contain reusable materials. ALL components with the exception of the HEPA filters may be disposed and/or recycled after they are known to be properly disinfected. ...
  • Page 55 OM0292 Page 55 of 57 Rev 4 November2021...
  • Page 56 OM0292 Page 56 of 57 Rev 4 November2021...
  • Page 57 OM0292 Page 57 of 57 Rev 4 November2021...

This manual is also suitable for:

Nu-640-400eNu-640-500eNu-640-600e

Table of Contents