QUICKGEL CHAMBER ONE - Instrument Use and Function Section 1 - Instrument Use and Function ® The Helena QuickGel Chamber is intended for the electrophoresis of samples on Quick- Gel agarose gels. Once electrophoresis is complete, the QuickGel Chamber provides for the drying of the gel.
QUICKGEL CHAMBER TWO - Principles of Operation Section 2 - Principles of Operation The gel is placed in the electrophoresis chamber, sample is applied, and current is applied from an external electrophoresis power supply at the recommended voltage for the recommended time interval. The sample migrates across the gel, separating into frac- tions.
QUICKGEL CHAMBER THREE - Precautions and Limitations Section 3 - Precautions and Limitations and germicidal agent. Observe where the specimens are used inside the instrument 3.1. The entire operator’s manual should be and confine cleaning to that area. Wipe up read and understood before attempting in- the residue.
QUICKGEL CHAMBER FOUR - Hazards Section 4 - Hazards 4.1. If the instrument is used in a manner not specified by this manual, the protection pro- vided by equipment design may be impaired. 4.2. This device contains very high voltages which can be extremely dangerous.
QUICKGEL CHAMBER FIVE - Controls Section 5 - Controls Electrophoresis Supply Input Red and Black Leads and Ports Power Indicator Switch Light Figure 5-1. QuickGel Chamber Controls...
59° to 80°F (15° to 27°C). signs of damage. If damage is found, notify 2. Place the instrument on a level, flat surface the shipping carrier and Helena Laboratories. near an external electrophoresis power sup- 5. Inventory all items and if any parts are ply.
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QUICKGEL CHAMBER SIX - Installation Instructions 4. Plug the red and black leads into the like colored ports on the top of the instrument by simultaneously squeezing the black tab at the end of each lead and inserting the lead into the appropriate port.
QUICKGEL CHAMBER SEVEN - Operating Instructions Section 7 - Operating Instructions 9. To dry a gel using the QuickGel Chamber: a. Install the dryer lid (section 7.1.1). 7.1. Instrument Operation b. If this is the final dry cycle of the test, en- 1.
QUICKGEL CHAMBER SEVEN - Operating Instructions 7.3. Results Refer to the procedure supplied with the gels for a complete discussion of results and their interpretation. 7.4. Cleanup The sample cups and applicator blades should be handled and disposed of as bio- hazards.
QUICKGEL CHAMBER EIGHT - Test Functions and Quality Control Section 8 - Test Functions and Quality Control Refer to the procedures supplied with the gels. A control should be run on each electropho- resis gel. Control data should be compared to the assay ranges printed on the assay sheet provided with the control.
QUICKGEL CHAMBER NINE - Performance Specifications Section 9 - Performance Specifications Test Types: Serum Protein Split Beta, CK, IFE, Acid Hemoglobin, Alkaline Hemoglobin Input Power: 100-240 Vac Frequency Rating: 47-63 Hz Current Rating: 3 Amps Fuses, located internally. Do not attempt to replace fuses.
10.1.3. Clean Applicator, Applicator in this manual, call Helena Laboratories for Base, and Rigid Antisera assistance. Template WARNING: The QuickGel Chamber is factory After each test, clean each reusable piece lubricated.
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QUICKGEL CHAMBER TEN - Maintenance, Troubleshooting, Warranty 3. Remove all remaining adhesive from the sheet has been stretched, it may be difficult to surface of the chamber floor using an Adhe- smooth out the wrinkles. If this occurs, re- sive Remover Pad provided with the contact place the contact sheet.
QUICKGEL CHAMBER TEN - Maintenance, Troubleshooting, Warranty 10.2. Troubleshooting If the recommended solutions should fail to solve a problem, call Helena Laboratories for as- sistance. Table 10-2. Troubleshooting Problem Possible Cause Solution No power to instrument Power cord unplugged Plug cord into proper wall outlet.
QUICKGEL CHAMBER ELEVEN - Symbology Section 11 - Symbology NOTE: The following symbols may be used in this manual, or on the instrument, to provide information necessary to the user, if applicable. Caution, electric shock hazard, high voltages capable of causing personal injury - shut down the instrument and unplug the power cord before touching - do not operate with the cover(s) removed Caution, heat hazard - allow heated components to cool before handling...
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