Helena Laboratories ProtoFluor-Z 2005 Operator's Manual
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Helena Laboratories ProtoFluor-Z 2005 Operator's Manual

Hematofluorometer
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Helena Laboratories
Operator's Manual
ProtoFluor-Z
Hematofluorometer
Cat. No. 2005, 110 Vac
Cat. No. 2006, 220 Vac

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Summary of Contents for Helena Laboratories ProtoFluor-Z 2005

  • Page 1 Helena Laboratories Operator’s Manual ProtoFluor-Z Hematofluorometer Cat. No. 2005, 110 Vac Cat. No. 2006, 220 Vac...
  • Page 2 ProtoFluor-Z Hematofluorometer Operator’s Manual Cat. No. 2005, 100 Vac Cat. No. 2006, 220 Vac...

  • Page 3: Table Of Contents

    ProtoFluor-Z Hematofluorometer Operator’s Manual Contents ONE – Instrument Use and Function ................1 TWO – Principles of Operation..................2 THREE – Precautions and Limitations ................ 4 FOUR – Hazards ......................6 FIVE – Controls and Displays ..................7 5.1. Controls ......................7 5.2.
  • Page 4 List of Figures 1-1. ProtoFluor-Z ………………………………………………………… Block Diagram ……………………………………………………… Front Panel Controls and Displays ………………………………. Back Panel Controls ……………………………………………….. 10-1 ProtoFluor-Z Back ………………………………………………….. 10-1 List of Tables Inventory …………………………………………………………….. 10-1 Troubleshooting……………………………………………………… 10-2...

  • Page 5: One - Instrument Use And Function

    ProtoFluor-Z ONE – Instrument Use and Function ONE – Instrument Use and Function The ProtoFluor-Z (Fig. 1-1) is a front-face protoporphyrin (ZPP) and determined its fluorometer designed absorption and fluorescence can be measurement of zinc protoporphyrin detected easily in whole blood by use of (ZPP) in whole blood.

  • Page 6: Two - Principles Of Operation

    ProtoFluor-Z TWO – Principles of Operation TWO – Principles of Operation The functional units of the ProtoFluor-Z are shown in figure 2-1. All user input is through the external panel controls and the on/off switch. Light from a quartz-halogen lamp is collected and filtered by a lens/filter system to produce light at 415 nm.
  • Page 7 ProtoFluor-Z TWO – Principles of Operation Photocell Figure 2-1 Block Diagram Page 3...

  • Page 8: Three - Precautions And Limitations

    ProtoFluor-Z THREE – Precautions and Limitations THREE – Precautions and Limitations 3.1. The entire operator’s manual may be significantly decreased when run should be read and understood before without reagent and moderately aged attempting instrument operation. samples without reagent generate unpredictable results. 3.2.
  • Page 9 3.24. Requirements handling Preventative maintenance must occur at biohazards: Due to potential biohazard Helena Laboratories. risk from human based components blood, CSF [Cerebrospinal Fluid], urine, Results may be affected prior to plasma, blood cells, etc.), guidelines detection by calibration and optical pertaining to Universal Precautions shall checks.

  • Page 10: Four - Hazards

    Do not operate any instrument with the cover removed unless instructed to do so by a qualified service technician directly representing Helena Laboratories, its subsidiaries or its distributors. 4.2. Do not attempt to operate the instrument without plugging the power...

  • Page 11: Five - Controls And Displays

    ProtoFluor-Z FIVE – Controls and Displays FIVE – Controls and Displays ----: in umol/mol mode H-35: in ug/dL mode, 35 hematocrit 5.1. Controls H-42: in ug/dL mode, 42 hematocrit BLANK/SCALE DOWN Button: Press 5.2. Display BLANK once to blank cover slip. When holding the PRESS TO SCALE button display shows...
  • Page 12 ProtoFluor-Z FIVE – Controls and Displays Figure 5-2. Back Panel Controls Page 8...

  • Page 13: Six - Installation Instructions

    5. Inventory all items: If any parts are voltage and frequency. Because the missing, recheck the packing materials power cord is the mains disconnect before notifying Helena Laboratories. device, the wall outlet used should be easily accessible. This information is Table 6-1 Inventory located on the serial number plate on the back panel of the instrument.
  • Page 14 ProtoFluor-Z SIX – Installation Instructions The instrument’s circuitry contains filters to reduce the effect of line voltage fluctuations: however, they should still be avoided. If the operator experiences difficulty, it may be necessary to install an isolation transformer. Page 10...

  • Page 15: Seven - Operating Instructions

    ProtoFluor-Z SEVEN – Operating Instructions SEVEN – Operating Instructions results (Incorrectly low or high) are obtained with hemolyzed specimens. 7.1 Specimen Collection and Handling Samples stored longer than Specimen: Whole blood collected with recommended have falsely an anticoagulant such as heparin, EDTA, increased or decreased results.

  • Page 16: Preparation And Warm-Up

    ProtoFluor-Z SEVEN – Operating Instructions Storage and stability: Specimens should be analyzed soon after collection This 30 minute warm-up warms the and before hemolysis occurs. Storage electronics in the instrument, but the light at 4 C for up to one week may be bulb is on only when MEASURE is acceptable, but as anticoagulated pressed.

  • Page 17: Calibrate Instrument

    ProtoFluor-Z SEVEN – Operating Instructions a problem. Call Helena Electronics The coverslip should now measure 000 Customer Services Department +/-1 indicating that the unit is zeroed on assistance. the coverslip. Note: If 000 +/- 1 is not achieved, repeat The instrument requires 5 sec. to recover from a reading.

  • Page 18: Patient Sample Assay

    ProtoFluor-Z SEVEN – Operating Instructions 6. Set the instrument to the published and place one drop of ProtoFluor Low value of the Calibrator. Use the Calibrator on it and insert it into the appropriate units of measure. instrument. The Calibrator value is printed on the 11.

  • Page 19: Summary Instructions

    ProtoFluor-Z SEVEN – Operating Instructions 4. Withdraw the sample holder and put a clean, lint-free coverslip on the sample 7.6 Summary Instructions holder. Note: Before initial operation of the 5. Press BLANK. instrument, read the instructions in Sections 7.2 to 7.5 for a complete 6.
  • Page 20 ProtoFluor-Z SEVEN – Operating Instructions 5. Press MEASURE. The reading will be 4. Remove all reagents, calibrators, and 000 +-1 if the instrument has properly samples from the refrigerator and allow blanked the coverslip. them to equilibrate to room temperature (about 30-45 minutes).
  • Page 21 ProtoFluor-Z SEVEN – Operating Instructions 4. Set calibration to the published value The low calibrator should be within +/-3 continuously holding down (+/-6 for position 2 (umol/mol)) of the PRESS TO SCALE button and press published value. If the reading is not either SCALE UP or SCALE DOWN until within this range, press MEASURE 2 to 3 the published value appears on the LED.

  • Page 22: Procedural Notes

    ProtoFluor-Z SEVEN – Operating Instructions 5. Put a clean coverslip on the sample 7.7. Procedural Notes holder. Place the holder in the instrument and press BLANK. 1. Be very careful to avoid contamination of the sample holder and the inside of the 6.
  • Page 23 ProtoFluor-Z SEVEN – Operating Instructions Make only one reading from each 13. When results are reported as a ratio prepared slide. If a repeat reading is of ZPP/Heme, results are not affected by necessary, place a fresh sample on a hemoglobin concentration or hematocrit, clean coverslip and make a second eliminating the need to correct for...

  • Page 24: Reference Values And Interpretation Of Results

    Interpretation of Results (35 or 42) Diagnosing Iron Deficiency A reference range study conducted by Helena Laboratories, which included 150 men and women in good health, provided the following normal range for zinc protoporphyrin: 30-80 umol ZPP/ml Heme 15-36 ug ZPP/dL whole blood*...

  • Page 25: Eight - Test Functions And Quality Contro

    ProtoFluor-Z EIGHT – Test Functions and Quality Control EIGHT – Test Functions and Quality Control The instrument automatically performs a self-test any time the power is turned on. The display should read PFZ. Should an error message appear on the display, turn the instrument off and on again.

  • Page 26: Nine - Performance Specification

    ProtoFluor-Z NINE – Performance Specifications NINE – Performance Specification Measurement Range: 0 to 600 umol ZPP/mol Heme Input Power: 110 Vac, 50/60 Hz, 50 Watts 220 Vac, 50/60 HZ, 50 Watts Fuses (2): 3-A/250-V slow blow (110 V unit) 1.6-A/250-V slow blow (220 V unit) Dimensions: 7-1/2 in.

  • Page 27: Ten - Maintenance, Troubleshooting, Warranty

    10.1. Maintenance do so may lead to decreased results. Preventative maintenance must occur 10.1.1. Fuse Replacement at Helena Laboratories. A decrease in results may occur prior to detection 1. Turn off the power and unplug the by calibration and optical checks.

  • Page 28: Troubleshooting

    ProtoFluor-Z TEN – Maintenance, Troubleshooting, and Warranty 10.2 Troubleshooting If the recommended solutions should fail to solve a problem, call Helena Laboratories for assistance. Table 10-1. Troubleshooting Problem Possible Cause Solution No power to instrument Power cord unplugged Plug cord into proper...

  • Page 29: Warranty

    ProtoFluor-Z TEN – Maintenance, Troubleshooting, and Warranty Unable to calibrate Lamp insufficiently Additional lamp cycles warmed Patient samples run low Optics dirty or overdue Contact Helena for maintenance Laboratories for Assistance Air blank > 3 Optics dirty or overdue Contact Helena for maintenance Laboratories for Assistance...

  • Page 30: Eleven - Symbology

    ProtoFluor-Z ELEVEN – Symbology ELEVEN – Symbology NOTE: The following symbols may be used in this manual, or on the instrument, to provide information necessary to the user, if applicable. Page 26...
  • Page 31 Operator’s Manual Catalog No. 2005, 110 Vac Catalog No. 2006, 220 Vac For Additional Information or Assistance, call your local distributor, or call Helena Laboratories at 1-800-231-5663 toll free. Helena Laboratories P.O. Box 752 Beaumont, TX 77704-0752 July, 2021 D6500037K...

This manual is also suitable for:

Protofluor-z 2006

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