Sorvall Surespin 630 Manual
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Sorvall Surespin 630 Manual

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SORVALL
Surespin 630
™
ROTOR
MANUAL
PN 79369-3

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  • Page 1  SORVALL Surespin 630 ™ ROTOR MANUAL PN 79369-3...
  • Page 2 IMPORTANT NOTICE regarding use of SORVALL ® Surespin™ 630 Swinging-Bucket Ultracentrifuge Rotor and changes to the instructions in the Rotor Manual, PN 79369 The Surespin™ 630 Rotor you have been supplied is slightly different than as described in O-RING O-RING the rotor manual.
  • Page 4 SLC-4000 Table of Contents OPERATING INSTRUCTIONS  ™ SORVALL Surespin 630 Swinging Bucket Ultracentrifuge Rotor Asheville, North Carolina U.S.A. PN 79369-3 Issued October 2001...
  • Page 5  ® SORVALL Surespin 630 Titanium Swinging Bucket Ultracentrifuge Rotor Data herein has been verified and is believed adequate for the intended use of the rotor. Because failure to follow the recommendations set forth in this manual could produce personal injury or property damage, always follow the recommendations set forth herein.

  • Page 6: Important Safety Information

     Important Safety Information Surespin Important Safety Information Certain potentially dangerous conditions are inherent to the use of all rotors. To ensure safe operation of this rotor, anyone using it should be aware of all safe practices and take all precautions described below and throughout this manual.

  • Page 7: Table Of Contents

    Table of Contents ® SORVALL Centrifuges Table of Contents Page Important Safety Information ........iii Chapter 1.
  • Page 8  Table of Contents Surespin List of Illustrations Figure Page 2-1. Cross-Section Showing Rotor Radii ......2-4 2-2.

  • Page 10: Chapter 1. Description

    Bucket Ultracentrifuge Rotor. If you require additional information regarding operation or maintenance, please contact Kendro for assistance. In the United States, call Kendro toll-free 1-800-522-7746; outside the ® United States, contact the nearest Kendro office (see back cover) or your local representative for SORVALL ® products. SORVALL product information is available on our internet web site at http://www.kendro.com...

  • Page 11: Accessories

     Description SORVALL Centrifuges b. Rotor with 36 ml Buckets: • Relative Centrifugal Force (RCF) at maximum speed: - at r (7.70 cm) ....77 408...
  • Page 12  Description Surespin Table 1-1. Accessories Supplied Quantity Catalog Number Description 1 set 79364 Buckets, 17 ml 79365 Buckets, 36 ml 4 boxes of 50 03126* Tubes, Polyallomer (17 ml) 8 boxes of 25 03141* Tubes, Polyallomer (36 ml) 79370* O-ring (17 ml buckets) or 79374* O-ring (36 ml buckets)

  • Page 14: Chapter 2. Operation

     Surespin Operation Chapter 2: OPERATION  This chapter contains the information necessary to prepare the Surespin 630 Rotor for operation and includes important safety information. Prerun Safety Checks W A R N I N G To ensure safe performance of the rotor, before every run you should: Failure to properly maintain your rotor can cause rotor failure with...

  • Page 15: Bucket Use

    If you question whether or not a rotor or bucket set should be used, contact Kendro  Service or your local representative for SORVALL products. When the rotor is removed from service at end of life, the buckets and caps that have been used with that rotor body should be removed from service also.

  • Page 16: Critical Speed

    If samples are routinely processed around 4°C or below, the rotor can be stored in a refrigerator or a cold room. If this is not possible, C A U T I O N the rotor may be able to be precooled in a SORVALL ® Ultracentri- Do not precool the rotor at fuge.

  • Page 17: Relative Centrifugal Force (Rcf) Determination

     Operation SORVALL Centrifuges Relative Centrifugal Force (RCF) Determination RCF refers to the force during centrifugation that moves the par- ticulate outward from the center of rotation. This force is propor- tional to the radial distance and the square of the rotor speed. The RCF value is determined by the following formula: RCF = 11.17 (r)
  • Page 18  Surespin Operation  Table 2-1. Surespin 630 RCF Values and K Factors 36 ml Buckets 17 ml Buckets Speed Speed maximum average minimum maximum average minimum (rpm) Factor (rpm) Factor 16.60 cm 11.65 cm 6.70 cm 16.60 cm 12.15 cm 7.70 cm 5 000 4 636...

  • Page 19: Calculation Of Sedimentation Times In Aqueous

     Operation SORVALL Centrifuges Calculation of Sedimentation Times in Aqueous (Non-Gradient) Solutions The time required to sediment a particle in water at 20°C through the maximum rotor path length (that is, the distance between and r ) can be estimated using the equation:...

  • Page 20: Calculation Of Sedimentation Times In Gradient Solutions

     Surespin Operation Calculation of Sedimentation Time in Gradient Solutions The time required to sediment a particle through a density gradient can be calculated using the following formula: where: sedimentation time in hours the clearing factor for the rotor (the value of is dependent on the gradient being used, the temperature of the gradient, and the density of the particle being sedimented).

  • Page 21: Rotor Installation

    For other tubes that can sure to do a test run for the desired be used with the rotor, refer to the SORVALL ® Product Guide.

  • Page 22: Cap And Bucket Assembly

     Surespin Operation Assemble the tube and bucket as follows (see Figure 2-3): 1. Make sure there is a film of vacuum grease (Catalog No. 65937) on each bucket cap O-ring, as well as a thin film of lubricating grease (Catalog No. 61556) on the fine threads on each bucket cap.

  • Page 23: Installing Buckets And Performing A Run

     Operation SORVALL Centrifuges 5. Insert each numbered bucket cap into the bucket with the same number, so that the cap threads seat on the bucket threads. Then, rotate each cap clockwise until the cap seats firmly inside the bucket, typically about 3/4 of a turn. When tightened prop- erly, the cap handle should be either parallel or perpendicular (approximately) to the slots on the bucket (see Figure 2-4).

  • Page 24: Centrifuge/Rotor Log

     Surespin Operation 6. Record the run data in the Ultraspeed Centrifuge/Rotor Log Book supplied (see Centrifuge/Rotor Log, below). 7. To remove the bucket caps at the end of the run, gently turn each cap handle in the direction indicated by the arrows, coun- terclockwise.

  • Page 26: Chapter 3. Maintenance

    Most decontaminated first, then washed. laboratory chemicals can be removed using the SORVALL ® tor Cleaning Kit (Catalog No. 92362) C A U T I O N...

  • Page 27: Inspection

     Maintenance SORVALL Centrifuges 2. Decontamination Ethylene oxide, a 2% glutaraldehyde solution, or ultraviolet radiation are the recommended methods of sterilization; how-  ever, the Surespin 630 can be autoclaved at temperatures up to 121°C if all of the parts (including O-rings) are disassembled and separated beforehand.

  • Page 28: Storage

    Kendro representative or returning it to the Kendro facility. There must be no radioactivity detectable by survey equipment. The SORVALL ® Product Guide contains descriptions of com- monly used decontamination methods and a chart showing method compatibility with various materials.
  • Page 29  Maintenance SORVALL Centrifuges Do not leave a loaded rotor locked inside a centrifuge that requires servicing. If, with a loaded rotor installed in the cham- ber, a centrifuge malfunction makes it so that the chamber door will not open by normal means, follow the Emergency Sample Recovery procedure found in your centrifuge operating instruc- tions manual to gain access to the rotor.
  • Page 30  Maintenance Surespin If equipment is received at Kendro facilities without a valid RMA Number on the outside of the shipping container and a completed Equipment Return Decontamination Form on file, the equipment will be treated as a potential contamination hazard, and will not be serviced until decontamination certification has been completed.

  • Page 32: Appendix

    APPENDIX...

  • Page 33: Chemical Compatibility Chart

    Surespin™ 630 Appendix Chemical Compatibility Chart Page 1 of 4: Chemicals through CHEMICAL – – – – – – – – – – – Acetaldehyde Acetic Acid (5%) – Acetic Acid (60%) Acetic Acid (Glacial) – Acetone Acetonitrile – – –...
  • Page 34 Appendix SORVALL ® Centrifuges Chemical Compatibility Chart Page 2 of 4: Chemicals through CHEMICAL – – – – – – – Chromic Acid (50%) Citric Acid (10%) – – – – – – – Cresol Mixture Cyclohexane – Deoxycholate –...
  • Page 35 Surespin™ 630 Appendix Chemical Compatibility Chart Page 3 of 4: Chemicals through CHEMICAL – – – – – – – – – Isobutyl Alcohol Isopropyl Alcohol – – Kerosene Lactic Acid (20%) – – – – – – – – –...
  • Page 36 Appendix SORVALL ® Centrifuges Chemical Compatibility Chart Page 4 of 4: Chemicals through CHEMICAL – – – – Rubidium Chloride Sodium Borate – – – – Sodium Bromide Sodium Carbonate (2%) Sodium Chloride (10%) – – – – – –...
  • Page 37 This warranty is void if the rotor is (i) operated or maintained in a manner contrary to the instructions in the manual for the rotor or ultracentrifuge in use, or (ii) used in a SORVALL ® Ultracentrifuge that has been modified without the written permission of Kendro.
  • Page 38  Surespin Index Index Accessories, 1-2 Ordering Information, 1-2, See Back Cover Autoclaving, 3-2 Overspeed Decal Replacement, 3-2 Balancing, Rotor, 2-8, 2-9 Precool, Rotor, 2-3 Bucket Installation, 2-10 Prerun Safety Checks, 2-1 Bucket Loading, 2-8 Radii, Rotor, 2-4 Centrifuge/Rotor Log, 2-11 RCF Values, 2-5 Chemical Compatibility, 2-3, See Appendix Relative Centrifugal Force (RCF), 2-4...
  • Page 39 NOTES...
  • Page 40 DECONTAMINATION INFORMATION CERTIFICATE Complete and attach to equipment BEFORE servicing (instructions on reverse) PLEASE PRINT DECONTAMINATION CERTIFIED BY _____________________________________________________ TITLE/POSITION ___________________________________________________ PHONE ___________________________ FAX ____________________________ DEPARTMENT _________________________________________________ INSTITUTION _____________________________________________ ADDRESS _________________________________________________________________ CITY _____________________________________________ STATE ____________________________________ ZIP _________________________________ INSTRUMENT ____________________________________________________________________ SERIAL NUMBER _____________________________________ ROTOR _________________________________________________________________________ SERIAL NUMBER _____________________________________ PART ______________________________________________________________________________________...
  • Page 41 INSTRUCTIONS When an instrument that has been used with radioactive, patho- and certification has been completed. If an instrument is received at genic, or otherwise hazardous materials requires servicing by Kendro our Service facilities and, in our opinion, poses a radioactive or personnel either at the customer's laboratory or at Kendro facilities, biological hazard, the sender will be contacted for instructions as to the following procedure must be complied with to insure safety of...
  • Page 42 1 (203) 270-2115 Tel.: 1 (203) 270-2080 1 (203) 270-2110 Fax: 1 (203) 270-2210 e-mail: info@kendro.com 1 (203) 270-2166 e-mail: info@kendro.com ® Or contact a local representative for SORVALL brand products. Visit our web site at http://www.kendro.com or http://www.kendro.de Rev. 04/01...

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