Related Manuals for Macherey-Nagel NucleoMag 96 PCR
Summary of Contents for Macherey-Nagel NucleoMag 96 PCR
- Page 1 MACHEREY-NAGEL User manual PCR clean-up n NucleoMag 96 PCR ® June 2021 / Rev. 04 www.mn-net.com www.mn-net.com...
- Page 2 1, rue Gutenberg – BP135 · 67720 Hoerdt Cedex · France Tel.: +33 388 68 22 68 E-mail: sales-fr@mn-net.com MACHEREY-NAGEL SAS (Société par Actions Simplifi ée) au capital de 186600 € Siret 379 859 531 00020 · RCS Strasbourg B379859531 · N° intracommunautaire FR04 379 859 531 Switzerland MACHEREY-NAGEL AG Hirsackerstr.
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Page 3: Table Of Contents
2.6 Elution procedures 3 Storage conditions and preparation of working solutions 4 Safety instructions 5 Protocol for clean-up of 50 μL PCR reactions 6 Appendix 6.1 Troubleshooting 6.2 Ordering information 6.3 Product use restriction / warranty MACHEREY-NAGEL – 06/2021, Rev. 04... -
Page 4: Components
Please contact Technical Service regarding information about changes of the current user manual compared to previous or updated revisions. ¹ For preparation of working solutions and storage conditions see section 3. MACHEREY-NAGEL – 06/2021, Rev. 04... -
Page 5: Product Description
The kit can also be used with other common separators. Magnetic separator Separation plate or tube ® NucleoMag SEP (MN REF 744900) Square-well Block (MN REF 740481) Tecan Te-MagS™ 1.5 mL tubes without lid (Sarstedt) MACHEREY-NAGEL – 06/2021, Rev. 04... -
Page 6: Adjusting The Shaker Settings
Shake the storage bottle well or place it on a vortexer shortly. Premixing magnetic beads with the binding buffer allows easier homogenous distribution of the beads to the individual wells of the separation plate. During MACHEREY-NAGEL – 06/2021, Rev. 04... -
Page 7: Elution Procedures
For some separators, high elution volumes might be necessary to cover the whole pellet. Elution is possible at room temperature. Yield can be increased by 10–15 % if elution is performed at 55 °C. MACHEREY-NAGEL – 06/2021, Rev. 04... -
Page 8: Storage Conditions And Preparation Of Working Solutions
4 x 96 preps 24 x 96 preps 744100.1 744100.4 744100.24 Buffer MP3 25 mL 100 mL 2 x 200 mL (Concentrate) Add 100 mL ethanol Add 400 mL ethanol Add 800 mL ethanol to each vial MACHEREY-NAGEL – 06/2021, Rev. 04... -
Page 9: Safety Instructions
IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell. BEI VERSCHLUCKEN: Bei Unwohlsein GIFTINFORMATIONSZENTRUM/Arzt anrufen. P 330 Rinse mouth. Mund ausspülen. For further information please see Material Safety Data Sheets (www.mn-net.com). Weiterführende Informationen finden Sie in den Sicherheitsdatenblättern (www.mn-net.com). MACHEREY-NAGEL – 06/2021, Rev. 04... -
Page 10: Protocol For Clean-Up Of 50 Μl Pcr Reactions
2 min separation Wash with MP2 Remove Square-well Block ® from NucleoMag 300 μL MP2 Resuspend: Shake 5 min at RT (Optional: Mix by pipetting up and down) Remove supernatant after 2 min separation MACHEREY-NAGEL – 06/2021, Rev. 04... - Page 11 Remove Square-well Block ® from NucleoMag 25–100 μL MP4 (Optional: Elute at 55 °C) Shake 5 min at RT (Optional: Mix by pipetting up and down) Separate 2 min and transfer DNA into Elution Plate U-bottom MACHEREY-NAGEL – 06/2021, Rev. 04...
- Page 12 Separate the magnetic beads by placing the Square-well Block on the NucleoMag SEP magnetic separator. Wait at least 2 min until all the beads have been attracted to the magnet. Remove and discard supernatant by pipetting. MACHEREY-NAGEL – 06/2021, Rev. 04...
- Page 13 Elution Plate U-bottom. Note: Yield can be increased by 15–20 % by using pre-warmed elution buffer (55 °C) or by incubating the bead / elution buffer suspension at 55 °C for 5 min. MACHEREY-NAGEL – 06/2021, Rev. 04...
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Page 14: Appendix
DNA by ethanol and elute / redissolve in Elution Buffer MP 4 applications buffer or water. Eluted DNA contains residual primers / primer dimers • Minimize amount of primers in PCR reaction mixture. Do not use higher volumes of binding buffer than specified. MACHEREY-NAGEL – 06/2021, Rev. 04... -
Page 15: Ordering Information
1 x 96 preps 744100.4 4 x 96 preps 744100.24 24 x 96 preps ® NucleoMag 744900 Square-well Blocks 740481 740481.24 Elution Plate U-bottom 740486.24 Self-adhering PE Foil 740676 50 sheets Visit www.mn-net.com for more detailed product information. MACHEREY-NAGEL – 06/2021, Rev. 04... -
Page 16: Product Use Restriction / Warranty
Please contact us if you wish to get an extra copy. There is no warranty for and MACHEREY-NAGEL is not liable for damages or defects arising in shipping and handling (transport insurance for customers excluded), or out of accident or improper or abnormal use of this product;... - Page 17 FITNESS FOR A PARTICULAR PURPOSE OR USE, MERCHANTABILITY, CONDITION, OR ANY OTHER MATTER WITH RESPECT TO MACHEREY-NAGEL PRODUCTS. In no event shall MACHEREY-NAGEL be liable for claims for any other damages, whether direct, indirect, incidental, compensatory, foreseeable, consequential, or special (including...
- Page 19 Plasmid DNA Clean up Viral RNA and DNA Protein High throughput Accessories Auxiliary tools...
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