OLS CASY TT Operator's Manual
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OLS CASY TT Operator's Manual

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CASY TT
CASY TT
CELL COUNTER & ANALYZER | Operators Guide
For life science research only. | Not for use in diagnostic procedures.

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Questions and answers

Trunzer
May 26, 2025

Hallo, wie kann ich gemessene Daten löschen?

0
1 comments:
Mr. Anderson
May 26, 2025

To delete measured data (setups) on the OLS CASY TT:

1. Use Select Capillary to choose the capillary whose setups you want to delete.
2. Press NEXT to move the input marker between setups tagged with *.
3. To tag a setup for deletion, press 2. The * will change to D.
4. To cancel a deletion, press 1. The D changes back to *.
5. Repeat the process to select all setups you want to delete.
6. Press ENTER to confirm deletion. A warning will appear.
7. Press ENTER again to permanently delete the tagged setups, or CANCEL to abort.

This answer is automatically generated

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Summary of Contents for OLS CASY TT

  • Page 1 CASY TT CASY TT CELL COUNTER & ANALYZER | Operators Guide For life science research only. | Not for use in diagnostic procedures.

  • Page 3: Table Of Contents

    Pulse Area Analysis ..........................12 1.2.3 Measuring Range Dynamics .........................13 Viability Determination using Electrical Current Exclusion ............14 Aggregation Correction ........................15 CASY TT ..............................15 Device configuration Measuring Unit ............................18 Measuring Capillary and External Electrode .................19 Pressure System and Power Supply ....................20 Control Panel ............................20 Startup Choosing the Location .........................22...
  • Page 4 Data Output Using CASY excell ......................58 7.13.1 Macros not Activated ..........................58 7.13.2 Data is not Transferred ...........................58 7.13.3 Using a Computer with a Multi-Core-Processor ..................58 Appendix Data Output via the Serial Interface ....................59 Technical Data on CASY TT .........................61 CASY TT...

  • Page 5: Prologue

    & tricks trouble-shooting Every effort has been made to ensure that all the information contained in the CASY TT Operators Guide is correct at the time of printing. However, OLS OMNI Life Science GmbH & Co KG reserves the right to make any changes necessary without notice as part of ongoing product development.

  • Page 6: Declaration Of Conformity

    The CASY TT is intended for life science research and must be used exclusively by laboratory professionals who are trained in laboratory techniques and have studied the instructions for use of this instrument. The CASY TT is not in- tended for use in diagnostic procedures.

  • Page 7: Conventions Used In This Operator's Guide

    Conventions Used in this Operator’s Guide VII. Conventions Used in this Operator’s Guide Text Conventions To impart information that is consistent and memorable, the following text conventions are used in this Operator’s Guide: Numbered Listing Describes the steps in a procedure that must be performed in the order listed.

  • Page 8: Handling Requirements

    Any leaks in the liquid management system must be repaired immediately. Contact your distributor or authorized service technician. When the CASY TT fluid system contains CASY ton, always ensure that it does not dry out, to prevent malfunction or damage to the fluid system and valves caused by salt crystals.

  • Page 9: Disposal Of The Instrument

    The instrument should also be decontaminated prior to shipping it for outside service or repairs. Components of your CASY TT which are marked with the crossed-out wheeled bin symbol are covered by the European Directive 2002/96/EC (WEEE). These items must be disposed of via designated collection facilities appointed by government or local authorities.

  • Page 10: Introduction

    The calibration of the CASY Cell Counter and Analyzer is factory certified and non-changeable by the user, offering a very high level of standardization. This guarantees that the results can be compared from user-to-user, from lab-to-lab, from day to day and from instrument-to-instrument. CASY TT Operator‘s Guide...

  • Page 11: Measuring Principle

    Measuring Principle Digital Pulse Processing 1.2 Measuring Principle The technology of CASY Systems combines a proven particle measurement technique, referred to as the “Electrical Sensing Zone” technique (ISO 13319), with a modern method of signal evaluation, the “Pulse Area Analysis”. 1.2.1 Digital Pulse Processing Pulse Area Analysis was developed in 1987 on the basis of Digital Pulse Processing (DPP), a tech-...

  • Page 12: Pulse Area Analysis

    524,288 measuring channels finally a diameter-linear size distribution with a resolution of 400 display channels is calculated. Electrodes Signal Analysis 1 million measures/sec. Measuring pore Assigned in 512.00 channels ⇒ Signal analysis in area, height, width and course of time Figure 2: Pulse Area Analysis. CASY TT Operator‘s Guide...

  • Page 13: Measuring Range Dynamics

    (pore size 45 μm) and the 60 μm capillary (pore size 60 μm) are offered. A CASY TT can be equipped with either the 150 μm capillary only or with all three capillaries (150 μm, 60 μm and 45 μm). The 45 μm capillary detects objects starting from 0.7 μm and therefore is used, for example, to analyze bacteria or small fungi, whereas the 60 μm capillary detects objects...

  • Page 14: Viability Determination Using Electrical Current Exclusion

    For more information on using the normalization and evaluation cursors, see section Setting the Measuring Range and the Cursor Positions and as well as videos and guides on www.cell- counting.de. CASY TT Operator‘s Guide...

  • Page 15: Aggregation Correction

    For more information on using aggregation correction and determining the aggregation factor, please visit www.cellcounting.de. 1.4 CASY TT The CASY TT combines a high degree of ergonomics with functionality. At the push of a button, the electronics: Control the measuring stand...

  • Page 16: Device Configuration

    Figure 5). Measuring capillaries (see Figure 5); the 150 µm capillary is installed on delivery, depending on your CASY TT version the 60 µm and 45 µm capillaries are delivered in their storage cups included in the accessory box. Protection shield (delivered with cable in the accessory box) Control panel with graphic display and 13 control keys (see Figure 6).
  • Page 17 Measuring Unit) Base Sample platform Precision Measuring Capillary Storage Container Rear view: Voltage selector Parallel port (DB25) Power Switch Power input Serial port, Data Socket (DB9) RS232 (DB9) socket Figure 5: Schematic of CASY TT, frontal and rear view Device configuration...

  • Page 18: Measuring Unit

    (see Chapter 6.4). The required CASY ton is sucked in from the storage container and then emptied into the waste container. The entire volume of the internal fluid system is approx. 2 ml. CASY TT Operator‘s Guide...

  • Page 19: Measuring Capillary And External Electrode

    The measuring capillaries should be cleaned using only the CASY clean agent specified by OLS OMNI Life Science. Other agents may destroy the measuring capillaries. OLS OMNI Life Sci- ence is not liable for any damage caused by using alternative cleaning agents.

  • Page 20: Pressure System And Power Supply

    On the left are the keys used to position the evaluation and the normalization cursors. All keys have a clearly defined pressure point. CASY TT Operator‘s Guide...
  • Page 21 Shifts cursor to the left. R-CURSOR: Positions right cursor. Figure 6: CASY TT, Control Panel For adjusting the contrast of the CASY Control Panel display, you will find an adjustment wheel lo- cated on the right side of the CASY Control Panel.

  • Page 22: Startup

    3. STARTUP Choosing the Location CASY TT is a precision measuring instrument. To ensure fault-free measuring operation, you must choose the location according to the following criteria: Place the instrument on a solid, dry work table that is exposed to neither strong mechanical vibrations nor high-volume noises (e.g., ultrasonic baths).

  • Page 23: Switch-On/-Off

    Removing the plug from the control panel when the measuring unit is switched to ON may result in damage to the graphic display electronics. OLS OMNI Life Science GmbH & Co. KG accepts no liability for damage caused by severing a live connection between the measuring unit and the control panel.

  • Page 24: Preparing For Measurement

    Press the START key to initiate a measurement.  To guarantee that the CASY cup is fixed, you can rotate the sample platform one turn so that the recess is no longer pointing forwards. This secures the cup. CASY TT Operator‘s Guide...
  • Page 25 Preparing for Measurement For checking the background a setup for the appropriate capillary must be loaded or generated. The dilution factor must be set to 1 since only filtered CASY ton is used for measuring the background. In addition, the cursor positions should be set to the scale limits to detect all potentially contaminat- ing particles.

  • Page 26: Maintenance And Care

    CASY clean, is available from OLS The system should be cleaned using only the CASY clean agent specified by OLS OMNI Life Sci- ence Never use aggressive cleaning fluids on the CASY Instrument. OLS OMNI Life Science ac- cepts no liability for damage caused by using other cleaning agents! 4.1 Weekly Cleaning Routine...

  • Page 27: Cleaning The Cylinders And The Sampling Chamber

    Cleaning the Cylinders and the Sampling Chamber 4.2 Cleaning the Cylinders and the Sampling Chamber Clean the surface of the CASY Instrument regularly using a damp microfiber cloth. It is important to remove salt solution spillages in the instrument immediately. To do this, raise the sample platform and remove it by pulling forwards.

  • Page 28: Operation Of Casy Tt

    MAIN MENU. Displayed to its right is the serial number of your CASY TT, the date and time. The lower border, also separated by a horizontal line, contains instructions on how the screen and individual keys operate.

  • Page 29: Main Menu - Select Setup

    Press ENTER again to load the setup displayed. If you do not wish to load the setup displayed, you can return to Setup Selection by pressing CANCEL. The setup parameters cannot be changed at this point. To edit a loaded setup, use the Edit Setup menu item described below (see Chapter 5.4). Operation of CASY TT...

  • Page 30: Main Menu - Edit Setup

    MAIN MENU – Edit Setup 5.4 MAIN MENU – Edit Setup Edit Setup opens the setup screen. All the parameters provided by the CASY TT to define the mea- suring conditions, display the size distribution, calculate and output the measuring results, can be edited in this screen.
  • Page 31 Debris/ml. The procedure for setting the normalization cursor is the same as that described for the evaluation cursor. The nor- malization cursor can also be modified using the control panel arrow keys while visually monitoring the size distribution. Operation of CASY TT...
  • Page 32 %Via percentage calculation. P.Vol: This parameter defines the mean volume of individual cells in your samples for the manual aggregation correction (see Aggr.Correct) in terms of femtolitres. The value is entered in exponential format. CASY TT Operator‘s Guide...
  • Page 33 ENTER. At this point, you can press CANCEL to return to the setup screen. The changes you have made to the measuring parameters will then be reset. Operation of CASY TT...
  • Page 34 If you try to overwrite a logged setup, a warning message will appear. You will be reminded that pressing ENTER will permanently delete this setup’s existing settings. Pressing CANCEL enables you to return to the Saving Setup screen to select a free setup for saving. CASY TT Operator‘s Guide...

  • Page 35: Main Menu - Select Capillary

    5.6 MAIN MENU – Set Date and Time Your CASY TT has an integral Real-Time-Clock. The Set Date and Time function opens the DATE AND TIME screen, in which you can set date and time. You have the option of changing the date display from EU format “DD.MM.YYYY”...

  • Page 36: Main Menu - System Settings

    You can step to the settings of the 4 parameters by pressing MENU. The required parameters are selected from the predefined values as indicated in the table using the 1 and 2 keys. If not used is selected, the corresponding position in the upper section of the size distribution screen remains empty. CASY TT Operator‘s Guide...

  • Page 37: Main Menu - Service Menu

    This command verifies that the evaluation electronics function properly and all calculations produced by the evaluation software are correct. Use the MENU and ENTER keys to select and start the individual commands. Pressing CANCEL enables you to exit the Service Menu. Operation of CASY TT...

  • Page 38: Performing And Evaluating Measurements

    Fitting (see Chapter 2.2) and selecting (see Chapter 5.5) the required measuring capillary where necessary. Installing the protection shield (see Chapter 3.4 and the CASY TT Customer Training Guide) Selecting an appropriate setup (see Chapter 5.3) or manually inputting the para meters (see Chapter 5.4).

  • Page 39: Measuring Samples And Displaying The Measuring Results

    Instructions for setting up the major applications can be found in the CASY TT Customer Training Guide.The example above shows the size distribution for an eukaryotic cell line. To the left of the left-hand normalization cursor appearing as a dotted line (< 7.6 µm), you will see the Debris Peak.

  • Page 40: Numerical Measuring Results

    This example shows the format of the numerical display for settings %Calculation = %Via and Aggr.Correct = Manual. Details on possible settings can be found in Chapter 5.4 Depending on your settings, some of the parameters visible in this example may not appear or alternative parameters are shown. CASY TT Operator‘s Guide...
  • Page 41 Numerical Measuring Results Measuring parameters in the header of the numerical display SET: The setup used for the measurement in NAME-## format. If the current setup is not stored, NOT SAVED will appear here. TIME: The time at which the measurement was performed. DATE: The date on which the measurement was performed.

  • Page 42: Points To Be Observed When Performing Measurements

    CASY System by OLS OMNI Life Science GmbH & Co. KG. Using alternative measuring solutions, cups and cleaning agents may lead to incorrect measuring results and cause damage to the fluid system of the CASY Instrument. OLS OMNI Life Science GmbH & Co. KG accepts no liability for damage caused by using alternative consumables.
  • Page 43 Points to be Observed when Performing Measurements Sample concentration For correct measurement results, the concentration of the diluted cell suspension in the CASY cup should not exceed certain maximum values. The concentration limit depends on the size of the measuring capillary: 150 µm measuring capillary: <...
  • Page 44 During the CLEAN cycle, fluid is flushed under pressure from inside the measuring capillary through the measuring pore into the sample cup. To prevent your sample becoming diluted or contaminated, replace it with a CASY cup containing freshly filtered CASY ton before you run a CLEAN cycle. CASY TT Operator‘s Guide...
  • Page 45 Points to be Observed when Performing Measurements Air bubbles The CASY System precisely monitors the incidence of “critical” air bubbles in the rising fluid column within the vertical tube. Large air bubbles affect the accuracy of the sample volume measurement and may therefore result in the cell concentration being measured incorrectly.

  • Page 46: Setting The Measuring Range And The Cursor Positions

    CASY ton, if necessary, and run another CLEAN cycle. If the error message reappears even when the storage container is sufficiently full, the pressure system may be malfunctioning or the internal tube system is blocked. In this case, contact your OLS OMNI Life Science GmbH & Co. KG- instru- ment representative.
  • Page 47 Setting the Measuring Range and the Cursor Positions Measuring range Select the measuring range so that even the largest anticipated cell aggregates can be recorded within the displayed size distribution. For most eukaryotic cells, a measuring range of between 0 – 40 µm and 0 –...

  • Page 48: Manual Cursor Control Using Control Panel Keys

    To export size distributions and measuring results into MS-Excel (PC) through a fully automated process, you can use CASY excell which is delivered with each CASY TT System. If you have set the Print Mode parameter to Auto, the data are automatically outputted after each measurement via interfaces specified in the current parameters (see Chapter 5.4).
  • Page 49 Outputting the Results The Graphic parameter applies only to the printed output. Depending on whether the Graphic parameter is set to On or Off, only the numerical results or both the numerical results and the size distribution are output. The numerical output has the following format: CASY Date: 15.04.09...

  • Page 50: Troubleshooting

    OLS OMNI Life Science GmbH & Co. KG to clarify further procedure. The same applies to the errors listed above, if they cannot be cleared by the actions described.

  • Page 51: Background Too High

    CASY Instrument and check if the sample chamber was shielded during the measurement using the protection shield. In case the described steps are not successful, contact your OLS representative for maintenance service by an OLS service technician. ...

  • Page 52: Message "Concentration Too High

    Ensure that only freshly filtered CASY ton is used. Best use a dispenser equipped with an appropriate filter ( OLS Cat. No. 05 651 751 001). For the 150 μm capillary the recommended pore size is ≤ 0.8 μm.

  • Page 53: Message "Large Air Bubble Detected

    If not, unscrew and remove the capillary again and put it into a beaker with 10 ml of CASY clean over night. Repeat the steps mentioned above starting with the ultrasonification step in CASY clean. In case the described steps are not successful, contact your OLS representative for maintenance service by an OLS service technician. ...

  • Page 54: Error Message Pr-5 Or Pr-8

    Put the storage container with filtered CASY ton back into its position and put a CASY cup with freshly filtered CASY ton under the capillary. Run three consecutive cleaning cycles with CASY ton. In case the described steps are not successful, contact your OLS representative for maintenance service by an OLS service technician. ...

  • Page 55: Control Panel Display Dark Or Light

    If the error message CL-1 is displayed during self test, the instrument has lost its calibration par- tially or completely (wrong check.sum). In this case the calibration needs to be re-loaded: Contact your OLS representative.  7.10 Error Message HW-3a If the error message HW-3a is displayed during selftest, the real time clock of the system is not run- ning.

  • Page 56: Poor Result Reproducibility

    Note that the CASY Instrument can only measure the concentration as present in the CASY cup to be analyzed. The calculation of the cell concentration in the original suspension depends on the cor- rectness of the dilution factor.  CASY TT Operator‘s Guide...

  • Page 57: Amount Of Cells Higher Than The Count In The Hemocytometer

    Amount of Cells Higher than the Count in the Hemocytometer 7.12 Amount of Cells Higher than the Count in the Hemocytometer The amount of cells counted with the CASY Instrument when using aggregation correction can be higher compared to the count in the hemocytometer, even when no aggregates can be seen in the hemocytometer.

  • Page 58: Data Output Using Casy Excell

    Changes in the CPU affiliation are not set up permanently. After restarting CASY excell you will have to repeat this procedure. To store these settings permanently, you may use programs that allow you to permanently store CPU affiliation settings. CASY TT Operator‘s Guide...

  • Page 59: Appendix

    400 size channels and all the values calculated from the current cursor settings. To enable the CASY TT to output the measured data via the serial interface, the Interface parameter must be set to Ser or P + S in the current setup. If the Print Mode is set to Auto, the data are automatically exported after every measurement.
  • Page 60 437 Peak Diameter in µm ###.## within evaluation cursor range 438 Check Sum XXXXXXXX 4 bytes hexadecimal Depending on your setup settings, some data tagged with an asterisk (*) are output as not calculated. CASY TT Operator‘s Guide...

  • Page 61: Technical Data On Casy Tt

    Technical Data on CASY TT 8.2 Technical Data on CASY TT Function: Electronic Cell Counter + Analyzer System for determining cell counts and cell size distributions, aggregation and viability. Operating Environment: Indoor use only Operational altitude: Up to 2000 m above sea level...
  • Page 62 ASCII, fully-automatic export into MS-Excel using CASY excell Technical features: Permanent, highly stable calibration for biological media – certified by OLS OMNI Life Science GmbH & Co. KG, identical for all CASY Systems. Recording of measuring results, including date, time, setup name, device serial number and all device settings.
  • Page 64 OMNI Life Science GmbH & Co. KG Karl-Ferdinand-Braun-Straße 2 28359 Bremen T +49 421 276 16 9-0 F +49 421 276 16 9-19 info@ols-bio.de For life science research only. www.ols-bio.de Not for use in diagnostic procedures. www.cellcounting.de...

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