Table 4. HVS Sequence Programming Voltages for Caliper NS12 A Chip
Sample
Buffer Waste
Sample Waste
Buffer
Figure 32. Example: Simple Sequence Wizard Electrokinetic Pinched Injection on a Chip.
6.
Click on the Step B tab to view its settings.
7.
Change the Step B name to "Injection."
8.
Set the Switch Step setting to Only Manually.
9.
Set Channels A-D to regulate D.C. voltages according to Table 4. You can either click and drag the DC Voltage
slider to set the values, or enter the values directly in the DC Voltage box. These settings are shown in
33.
10. Press Apply to upload the settings to the HVS.
11. Choose File > Save As and name the file.
46
Reservoir
Load (V)
A
-600
B
-1500
C
402
D
-792
Step A – Loading
Inject (V)
61
-100
-11
-1500
Figure