MSD MESO SECTOR S 600 User Manual
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MSD MESO SECTOR S 600 User Manual

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USER MANUAL
®
MESO
SECTOR S 600
ww w. mes os c a le.com
®

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Summary of Contents for MSD MESO SECTOR S 600

  • Page 1 USER MANUAL ® MESO SECTOR S 600 ww w. mes os c a le.com ®...
  • Page 2 No part of this Manual may be duplicated, reproduced, stored in a retrieval system, translated, transcribed, or transmitted in any form or by any means without the express prior written permission of MSD. This Manual shall be returned to MSD within two (2) business days following any request by MSD.

  • Page 3: Table Of Contents

    Power Switch and Input/Output (I/O) Panel ..................27 3.5.10 Halt Button (on some configurations) ....................28 Microsoft Windows-Compatible Workstations ....................29 MSD DISCOVERY WORKBENCH Software ..................... 29 Operational Modes ............................30 Image Readout ............................. 30 3.10 MESO SECTOR Demonstration Plate ......................31 3.11...
  • Page 4 Run ................................40 5.3.1 Operations ............................. 41 Results ................................ 42 Using the SECTOR S 600 ............................44 Single Plate Run ............................44 Stack Run—Multiple Plates .......................... 44 6.2.1 Loading the Stack Tube ........................44 6.2.2 Unloading the Stack Tube ....................... 46 Robotics Integration .............................
  • Page 5 Figure 3:13 Loading a demonstration plate ........................32 Figure 5:1 Instrument Status window..........................36 Figure 5:2 MSD DISCOVERY WORKBENCH desktop icon ....................37 Figure 5:3 SECTOR S 600 icon ............................37 Figure 5:4: MSD DISCOVERY WORKBENCH splash screen ....................38 Figure 5:5 SECTOR S 600 Window: Setup, Plate Summary, and Operations areas ..............
  • Page 6 Introduction...

  • Page 7: Introduction

    ® sensitivity, speed, dynamic range, and convenience. MSD develops, manufactures, and markets detection instrument systems as well as a line of assay kits for use with these instruments, a ® proprietary line of reagents, and custom microplate printing and assay development services. MSD MULTI-ARRAY microplates are available in 96- and 384-well formats with standard or high-binding surfaces.

  • Page 8: Warnings And Caution Symbols

    Warnings and Caution Symbols WARNING General warnings advise operators of potential hazards and highlight the procedures or information necessary to avoid personal injury during use of the SECTOR S 600. Symbol Explanation Risk exists for a mechanical, chemical, or safety hazard Risk exists for an electrical hazard Risk of exposure to biohazards CAUTION...
  • Page 9 Safety Information...

  • Page 10: Safety Information

    WEEE Compliance 2.1.1 The MESO SECTOR S 600 was placed on the market after 2005 in compliance with European Union (EU) directive 2012/19/EU, the Waste Electrical and Electronic Equipment (WEEE) Directive. For all inquiries regarding recycling of shipping materials and instrument disposal, contact MSD Instrument Service.

  • Page 11: Hazardous Substances

    Methods for the Restriction of the Use of Hazardous Substances in Electrical and Electronic Products), MSD has designated an environmental protection use period of 50 years for the MESO SECTOR S 600 instrument when used under normal operating conditions. Table 2:1 indicates parts containing hazardous substances.
  • Page 12 Symbol/Label Description This symbol indicates a measurement or requirement in Volts. This symbol indicates a measurement or requirement in Amperes, often referred to as Amps. This symbol indicates electric current is in the form of alternating current (AC). This symbol indicates placing a toggle switch in this position will place the instrument in a powered on state.

  • Page 13: General Operation

    General Operation Once initialized, the SECTOR S 600 instrument enters standby mode and maintains the CCD camera at its set temperature. The instrument is ready to read plates at any time. The SECTOR S 600 is designed to run indefinitely in standby mode. WARNING: The instrument must be operated with all covers in place.

  • Page 14: Electrical

    WARNING: Although the SECTOR S 600 is shielded and grounded, laboratory personnel should never remove any instrument covers that would expose electrical circuits. Only authorized MSD Service personnel should perform repairs to the interior of the SECTOR S 600. strongly...

  • Page 15: Chemical And Biological

    Contact MSD Scientific Support to obtain safety data sheets (SDS) for MSD plates and reagents. Use personal protective equipment recommended by your facility when handling any of these reagents. WARNING: Samples, user reagents, or controls used in assays may be infectious or biohazardous.

  • Page 16: Mechanical

    The SECTOR S 600 instrument weighs 124 lbs (56 kg). Be careful to use proper technique when lifting it to minimize the risk of injury. Two or more people should lift from beneath the instrument. Please contact MSD Instrument Service before attempting to move the instrument.

  • Page 17: Esd Sensitivity

    Changes or modifications not expressly approved by MSD may void the warranty. The operator shall use any special accessories provided with the equipment such as the power supply or shielded cables that are necessary for compliance with FCC standards.

  • Page 18: Decontamination Prior To Shipping Or Servicing

    WARNING: Prior authorization must be obtained before the instrument and/or its accessories are shipped to MSD. Authorization is contingent upon completion of the MSD Instrument Decontamination Certification Form and issuance of a Returned Merchandise Authorization (RMA) number by MSD Instrument Service.
  • Page 19 System Description...

  • Page 20: System Description

    Plate Compatibility The SECTOR S 600 is compatible with 96- and 384-well single-spot and MULTI-SPOT SECTOR plates (4-, 7-, and 10-spot plates). MSD plates are designed to be read only once and may not be reused. System Components A standard SECTOR S 600 system consists of the following...

  • Page 21: The Sector S 600 Instrument

    One Stacker Cover Plate (1, not shown) • Instrument status LEDs • Power switch and I/O panel (not shown) • Motion Halt button (on some configurations)  Additional stack tubes, single plate adapters, and stacker cover plates may be purchased from MSD. System Description...

  • Page 22: Ccd Camera And Telecentric Lens

    MSD barcode label that uniquely identifies the plate and allows the SECTOR S 600 to detect the type of plate being run. If desired, the user may apply a custom barcode label either on the same side of the plate as the MSD barcode or on one of the short sides of the plate.

  • Page 23: Multi-Array Plate Input/Output Stacker

    Stack Tubes 3.5.5 Stack Tubes allow the semi-automated processing of multiple plates in sequence. MSD offers standard-capacity (standard) and high capacity (extended) stack tubes (Table 3:1) for the SECTOR S 600. When using stack tubes, ensure that the single plate adapter has...

  • Page 24: Figure 3:4 Input (Right) Stacker Interface Plate

    Figure 3:4 Input (right) stacker interface plate Figure 3:5 Fully loaded standard and high capacity stack tubes System Description...

  • Page 25: Single Plate Adapter

    Single Plate Adapter 3.5.6 The SECTOR S 600 has the ability to read single plates placed manually into a single plate adapter. When inserting the single plate adapter into the stacker, ensure that the adapter is fully seated on the stacker interface plate. Figure 3:6 (right side of image) shows a single plate properly loaded in the single plate adapter.

  • Page 26: Status Leds

    Status LEDs 3.5.8 The status LEDs built into the camera tower indicate the current operational mode of the SECTOR S 600 (Figure 3:7). • Steady blue: Instrument is in standby mode • Blinking blue: Instrument is currently reading a plate •...

  • Page 27: Power Switch And Input/Output (I/O) Panel

    Power Switch and Input/Output (I/O) Panel 3.5.9 The I/O panel of the SECTOR S 600, located on the rear right side of the SECTOR S 600, includes the instrument’s power switch, fuse drawer, and connectors for power, USB and robotic halt activation cables (Figure 3:8).

  • Page 28: Halt Button (On Some Configurations)

    The halt switch should never be obstructed or restricted from access. The halt function can also be accessed through the rear I/O panel to enable external safety devices to halt the motion of the SECTOR S 600. Please contact MSD Scientific Support for more information on this feature.

  • Page 29: Microsoft Windows-Compatible Workstations

    Microsoft Windows-Compatible Workstations Computer workstations are configured by MSD for use with the SECTOR S 600 and come preloaded with MSD DISCOVERY WORKBENCH application software (see Section 3.7 MSD DISCOVERY WORKBENCH Software). The workstation includes a personal computer, display, keyboard, and mouse. The instrument computer and its software are an integral part of the system, configured at the manufacturer, and should only be modified by an MSD Service Engineer.

  • Page 30: Operational Modes

    Operational Modes The SECTOR S 600 can be configured as a stand-alone workstation or integrated into robotic systems using the single plate adapter provided. The single plate adapter replaces the input stack tube and can serve as the load/unload location when using a robot for loading plates or when loading a single plate manually.

  • Page 31: Meso Sector Demonstration Plate

    It should be used to verify the function of the SECTOR S 600 without the need for liquid reagents. The demonstration plate consists of an electronic circuit board housed in a plastic carrier in the shape of a standard plate. The circuit board for the SECTOR S 600 has six separate sectors, simulating the structure of MSD MULTI-ARRAY plates (Figure 3:12) as read by the SECTOR S 600.

  • Page 32: Uninterruptible Power Supply

    To avoid interference from voltage transients, the computer and instrument should be powered by the same electrical circuit. This can be accomplished by plugging the computer and the instrument into the battery backup outlets on the uninterruptible power supply (UPS) supplied with the instrument. Please contact MSD Scientific Support if you have questions or need assistance.
  • Page 33 Installation...

  • Page 34: Installation

    4 Installation A qualified MSD Service Engineer must install and configure the SECTOR S 600 before use. Installation includes setup, connection of the instrument to its computer system, and verification that the instrument is functioning properly. Only the computer provided with the instrument and configured by an MSD Service Engineer should be used to operate the SECTOR S 600.
  • Page 35 Quick Start...

  • Page 36: Quick Start

    The SECTOR S 600 has a cooled CCD camera that needs to be brought to operating temperature before plates are read. To initiate the cooling process, the SECTOR S 600 must be turned on, the MSD DISCOVERY WORKBENCH software must be started, and the SECTOR S 600 Tools must be running (see steps 1–4 below).

  • Page 37: Start-Up

    Log on to the Windows operating system. To do this, enter Administrator as the user login name and MsdAdmin as the password. (This is the default login shipped with the system; you may change this login/password or add additional Windows user accounts.) If DISCOVERY WORKBENCH does not start automatically, double-click the MSD DISCOVERY WORKBENCH icon on the Windows desktop (Figure 5:2).

  • Page 38: Setup

    5:4) will display while the instrument is initializing. If the instrument has just been powered-up, the cooling process will take approximately 45 to 60 minutes. Figure 5:4: MSD DISCOVERY WORKBENCH splash screen Setup On the left side of the SECTOR S 600 window, users can view and edit the Setup selections for the demonstration plate read (Figure 5:5).

  • Page 39: Figure 5:5 Sector S 600 Window: Setup, Plate Summary, And Operations Areas

    Select the Read __ Plate(s) checkbox and enter 1 in the text field. Figure 5:5 SECTOR S 600 Window: Setup, Plate Summary, and Operations areas Place the single plate adapter on the input (right) stacker interface plate (Figure 5:6). Figure 5:6 Stacker cover over the output port with empty single plate adapter in the input port Quick Start...

  • Page 40: Run

    Figure 5:7 MESO SECTOR demonstration plate in the single plate adapter NOTE: If the SECTOR S 600 is unable to read the barcode on the demonstration plate (or if no barcode label is present), the plate will be skipped and ejected from the instrument. Please contact MSD Scientific Support for assistance.

  • Page 41: Operations

    Operations 5.3.1 The Pause, Stop, Eject, and Retract buttons operate the motion control system that moves plates through the instrument. • Pause. Pauses the plate read. Selecting Pause again resumes the read. • Stop. Stops the current run and ejects any plate inside the instrument. •...

  • Page 42: Results

    Results The software provides several options for viewing results. Select View  Layer Views from the menu or select a layer from the Layer menu on the toolbar. Refer to the DISCOVERY WORKBENCH User’s Guide for details on data viewing options. If you observe the data as it is acquired in Color Map view, bright wells should display numbers above 2000 and dark wells should display numbers below 200.
  • Page 43 Using the SECTOR S 600...

  • Page 44: Using The Sector S 600

    WORKBENCH User’s Guide for detailed instructions on running MULTI-ARRAY plates on the SECTOR S 600 system. NOTE: This section assumes that a qualified MSD Service Engineer has installed and configured the instrument, and that the user has run a demonstration plate as described in the previous section.

  • Page 45: Figure 6:1 Loading Multi-Array Plates Into Stack Tube

    NOTE: A label on the inside of the stack tube reminds users of the correct orientation for loading plates. The stack tube itself will only fit on the interface plate in one orientation. Slide the stack tube carefully over the top of the stack of plates such that the plates slide into the tube. Once the stack tube base is flush with the stable surface, all but the extra plate will be loaded into the stack tube (Figure 6:1).

  • Page 46: Unloading The Stack Tube

    The SECTOR S 600 instrument can be integrated with a robotics system for loading and unloading plates using the Remote Instrument Mode, which allows the instrument to be used as a component through its remote interface. A Remote Instrument Manual that provides descriptions of the commands recognized by the MSD software is available upon request. Contact Scientific Support for more information on robotics integration.
  • Page 47 Maintenance...

  • Page 48: Maintenance

    7 Maintenance This chapter contains basic maintenance instructions for the SECTOR S 600 and some components. A qualified MSD Service Engineer should perform all other maintenance procedures not described in this section. WARNING: Opening the instrument to perform maintenance incurs risk of mechanical and electrical harm.

  • Page 49: Adapter And Stack Tube Cleaning

    Instrument Decontamination Contact MSD Scientific Support for a detailed protocol for instrument decontamination. NOTE: The SECTOR S 600 must be decontaminated prior to shipping the instrument back to MSD. Contact MSD Scientific Support prior to shipping for instructions and requirements.
  • Page 50 Appendix...

  • Page 51: Appendix

    Disable the antivirus program temporarily to see if that is the issue. There are several the plate after clicking the methods: Incompatible anti-virus Run button. Exclude the file: C:\Program Files\MSD Discovery Workbench\lib\prefs.properties software has been installed on from scanning. the instrument computer. Disable real-time scanning.
  • Page 52 In the alphabetical list of services, right-click SQL Server (MESOSCALE2) and select Restart. Error 8906 Invalid Plate A discontinued plate or a plate Discontinued plates cannot be run. In addition, plates made for other MSD instruments may Type configuration exclusive to be incompatible with the SECTOR S 600 instruments.
  • Page 53 Turn off the SECTOR S 600. Communications failure computer. Wait 30 seconds. Turn on the SECTOR S 600. Restart the computer. Wait five minutes before starting the MSD DISCOVERY WORKBENCH software. If the problem is not resolved, contact Scientific Support. Appendix...

  • Page 54: Specifications

    3.5A–2.8A @ 100–240 V~ Class I, Installation (Overvoltage) Category II Device Fuses Electrical fuses are only accessible by MSD Service Engineers and are not user-serviceable. The information below is provided for reference. Main Fuses F1 and F2: T4.0 A, 250 V, 100–240 V~...

  • Page 55: Sector S 600 Physical Dimensions

    Plate Specifications 8.2.8 MSD MULTI-ARRAY and MULTI-SPOT plates are designed to comply with the mechanical dimensions of the proposed standards of the Society of Biomolecular Screening. Contact MSD Scientific Support for further details.
  • Page 56 Technical Support...

  • Page 57: Technical Support

    9 Technical Support MSD provides excellent and timely support for all authorized users of SECTOR S 600. We welcome and carefully consider all bug reports and suggestions for improvements to future versions. We will work with you to resolve any problems you may encounter.
  • Page 58 MESO SCALE DISCOVERY, MESO SCALE DIAGNOSTICS, MSD, MSD GOLD, DISCOVERY WORKBENCH, MULTI-ARRAY, MULTI-SPOT, QUICKPLEX, SECTOR, SECTOR PR, SECTOR HTS, SULFO-TAG, R-PLEX, S-PLEX, U-PLEX, V-PLEX, STREPTAVIDIN GOLD, MESO, www.mesoscale.com, SMALL SPOT (design), 96 WELL 1, 4, 7, 9, & 10-SPOT (designs), 384 WELL 1 & 4-SPOT (designs), MSD (design), R-PLEX (design), S-PLEX (design), U- PLEX (design), V-PLEX (design), It’s All About U, and SPOT THE DIFFERENCE are trademarks and/or service marks of Meso Scale Diagnostics, LLC.

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